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All Journal Zuriat JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA

Yuyu S Poerba

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Author-ID : 342938

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science

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Induksi dan Karakterisasi Pisang Mas Lumut Tetraploid Poerba, Yuyu s; ,, Witjaksono; Ahmad, F.; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Triploid banana breeding could be achieved by crossing tetraploid to diploid banana cultivars. The availaibility of tetraploidbananas is naturally very limited. One of methods to produce tetraploid bananas is inducing tetraploid plants usingchemicals that inhibit spindle fiber formation during mitosis such as oryzalin or colchicine. The present research is aimed toproduce and characterize tetraploid Pisang Mas Lumut (Musa acuminata, AAAA genome) induced by in-vitro oryzalintreatment from diploid Pisang Mas Lumut. Fifteen in vitro-cultured shoots were treated wih oryzalin at of 60ÃÂ¼M for 7days in a liquid MS basal medium with addition of 2 mg/l BA. After 6 sub-cultured, 54 shoots and 104 plantlets wereproduced. Forty seedlings were then survived and grew well and healthy. Ploidy identification of induced Pisang MasLumut was conducted using Flowcytometer at seedling stage and repeated at reproductive phases for 2 cycles. The resultsshowed that oryzalin treatments produced tetraploids at a frequency of 30%, diploid at 65% and mixoploid at 5%. Thetetraploid plants have longer and thicker pseudostems, produce fewer suckers, drooping and larger leaves, and broader fruitscompared to its diploid control at harvest time. The tetraploid plants have similar genetic properties with their diploidcontrols as shown by genetic identity of 0.90-0.97.Key words: Pisang Mas Lumut, Musa acuminata, AA, oryzalin, tetraploid

PENGARUH MUTAGEN ETIL METAN SULFONAT (EMS) TERHADAP PERTUMBUHAN KULTUR IN VITRO ILES-ILES {Amorphophallus muelleri Blume) Poerba, Yuyu S; Leksonowati, Aryani; Martanti, Diyah
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Amorphophallus muelleri Blume (Araceae) is one of 27 Amorphophallus species occur wild in Indonesia (Sumatera, Java, Floresand Timor). The species is valued for its glucoman content for use in food industry (heathy diet food), paper industry, pharmacyand cosmetics. The cultivation of A. muelleri is hampered by limited genetic quality of seed. The species is triploid (2n=3x=39),the seed is developed apomictically. and pollen production is low. The species is only propagated vegetatively. This may explainthat the species is difficult to breed conventionally and genetic variabillity in the exiting landaraces cultivars is rather limited.Induced mutation using ethyl methan sulfonate is one of techniques to increase genetic variation. The present research is aimed todetermine Lethal Dosage (LD) 50% and 75% of EMS and to study effects of EMS on growth of A, muelleri in vitro cultures for usein induced mutation program. Results of the experiment showed that LD-50 and LD-75 was observed at 0.875% EMS and 0.5%EMS. respectively. Number of shoot, and percentage of rooting culture were decreasing as EMS level concentration increases.

MIKROPROPAGASI TANAMAN ILES-ILES {Amorphophallus muelleri Blume) Imelda, Maria; Wulansari, Aida; Poerba, Yuyu S
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

In Indonesia, iles-iles (Amorphophallus muelleri Blume) has not yet been cultivated intensively; their cultivation area is still limited. This species contains high glucomannan, which is useful as food diet, paper pulp, textile, paint, film-negative, celluloid and cosmetic industry. The cultivation of A. muelleri is hampered by limited genetic quality of plant.The species is triploid (2n=3x=39), the seed is developed apomictically, and pollen production is low.This may explain that the species is difficult to breed conventionally and genetic variability in the existing landraces cultivars is rather limited. Genetic variability of this plant is therefore can be achieve by induced mutation through tissue cultures for use in breeding program to develop better cultivars.Developing an efficient and effective micropropagation of the species is therefore important for use in the genetic improvement program.In other hands, the prospect for development and export of iles-iles is high since the demand from Japan alone has not been fulfilled. Propagation of iles-iles is generally done by splitting tubers, bulbils or leaf cuttings, but this method can not yield planting materials in large quantities within a relatively short time. In this research, young shoots which had just appeared from tubers were used as a source of explants. Sterilization of the explants was carried out in 0.05 % HgCl, solution for 20 min, rinsed several times with sterile distilled water and then cultured on Murashige and Skoog (MS) medium containing 0.1-0.2 mg/1 Thidiazuron (TDZ), 0.5-1.0 mg/1 Benzylaminopurine (BAP) and 0.5-1.0 mg/1 Kinetin (KIN) singly or in combination. Acclimatization of plantlets was done on 3 kinds of media namely (A), soil + compost, (B) soil + compost.+ cocopeat, and (C) soil + cocopeat. The results showed that the best medium is MS containing 0.2 mg/1 TDZ and 0,5 mg/1 BAP for in vitro shootbuds induction and proliferation of iles-iles, while MS without plant growth regulators is suitable for shoot growth and root formation and soil + compost + cocopeat for acclimatization of plantlets.

KERAGAMAN FENOTIPE RAPD Santalum album L. DIPULAU TIMOR BAGIAN TIMUR Poerba, Yuyu S; Wawo, Albert H; Yulita, KS
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Santalum album L. (sandalwood/cendana) is known as one of medicinal and aromatic tree species in Indonesia. The species is valued for its quality light wood timber and for its medicinal properties.The species has been overexploited and is considered as vulnerable plant species.The present study aimed to assess genetic diversity and to estimate genetic relationship among 58 accessions of plant germplasm collection using Random Amplified Polymorphic DNA (RAPD).Two RAPD primers generated 34 scorable bands with 97.06% of them were polymorphic. Clustering analysis was performed based on RAPD profiles using the UPGMA method.The range of genetic dissimilarity value among species was from 6% to 91%, while the range of genetic distance between populations was from 1.89% and 26.88%.These values showed that 5.album from Eastern part of Timor was genetically diverse populations.Within the 12 populations, there were 9 banding patterns recorded from primer OPA 16 and 12 banding patterns from primer OPB 12, suggesting that OPB 12 was more sensitive than that of OPA 16 to show variation within the sample used.

INDUKSI MUTASI KULTUR IN VITRO Amorphophallus muelleri Blume DENGAN IRRADIASI GAMMA Poerba, Yuyu S; IMELDA, MARIA; WULANSARI, AIDA; MARTANTI, DIYAH
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Amorphophallus muelleri Blume (Araceae) is valued for its glucoman content for use infood industry (heathy diet food), paper industry, pharmacy and cosmetics. The cultivationof A. muelleri is hampered by limited genetic quality of seed. The species is triploid(2n=3x=39), the seed is developed apomictically, and pollen production is low. Thespecies is only propagated vegetatively. This may explain that the species is difficultto breed conventionally and genetic variability in the existing landraces cultivars israther limited. Conservation of this species, therefore, is important for availability of thespecies in the future use of this plant. The objective of present research is to increasegenetic variation by induce mutation using gamma-rays irradiation of shoot culturesof A. muelleri and to identify DNA polymorphism induced by gamma irradiation usingrandom amplified polymorphic DNA (RAPD), so the mutants produced can be used forbreeding purposes and for conservation program. Results of the experiment showedthat gamma irradiation less than 5 gray was effective to induce mutation of A. muelleri.Four RAPD primers generated 35 scorable bands with 100% polymorphic bands. Sizeof the bands varied from 350bp to 2.0kbp. Clustering analysis was performed based onRAPD profiles using the UPGMA method. The range of genetic distance among individualgenotypes was from from 0.00 to 0.72, while genetic variance of the population was0.21 + 0.13. The eighteen genotypes were proof to be mutants. The mutants producedin this experiment could be used as new germplasms for breeding purposes as well asfor use in conservation strategy

INDUKSI DAN KARAKTERISASI PISANG MAS LUMUT TETRAPLOID Poerba, Yuyu s; ,, Witjaksono; Ahmad, F.; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2082

Triploid banana breeding could be achieved by crossing tetraploid to diploid banana cultivars. The availaibility of tetraploidbananas is naturally very limited. One of methods to produce tetraploid bananas is inducing tetraploid plants usingchemicals that inhibit spindle fiber formation during mitosis such as oryzalin or colchicine. The present research is aimed toproduce and characterize tetraploid Pisang Mas Lumut (Musa acuminata, AAAA genome) induced by in-vitro oryzalintreatment from diploid Pisang Mas Lumut. Fifteen in vitro-cultured shoots were treated wih oryzalin at of 60Î¼M for 7days in a liquid MS basal medium with addition of 2 mg/l BA. After 6 sub-cultured, 54 shoots and 104 plantlets wereproduced. Forty seedlings were then survived and grew well and healthy. Ploidy identification of induced Pisang MasLumut was conducted using Flowcytometer at seedling stage and repeated at reproductive phases for 2 cycles. The resultsshowed that oryzalin treatments produced tetraploids at a frequency of 30%, diploid at 65% and mixoploid at 5%. Thetetraploid plants have longer and thicker pseudostems, produce fewer suckers, drooping and larger leaves, and broader fruitscompared to its diploid control at harvest time. The tetraploid plants have similar genetic properties with their diploidcontrols as shown by genetic identity of 0.90-0.97.Key words: Pisang Mas Lumut, Musa acuminata, AA, oryzalin, tetraploid

Reproduction of corn lesion nematode on maize and white clover callus tissue cultures Yuyu S Poerba
Zuriat Vol 2, No 2 (1991)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v2i2.6731

Co-Authors ,, Witjaksono ,, Witjaksono Diyah Martanti, Diyah F. AHMAD IMELDA, MARIA Imelda, Maria Leksonowati, Aryani Tri Handayani Wawo, Albert H Wulansari, Aida Wulansari, Aida Yulita, KS

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