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PENYIMPANAN SERBUKSARI PISANG LIAR Musa acuminata Colla UNTUK MENDUKUNG PROGRAM PEMULIAAN PISANG BUDIDAYA [Pollen Storage of Wild Varieties of Musa acuminata Colla Ensuring Pollen Source for Cultivated Banana Breeding Program] Rachman, Erlin; Poerba, Yuyu Suryasari; Ahmad, Fajaruddin
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Research on pollen storage of four varieties and one subspecies of wild banana, Musa acuminata Colla, was carried out to support genetic improvement of banana cultivars through hybridization. The four wild varieties, breviformis, zebrina, malaccensis, bantamensis and a subspecies banksii cultivated at Cibinong Science Center (CSC-LIPI), Cibinong. Anthers were collected, put on bread paper,dried on hot plate at 40ÂºC for about two hours, open dried at room temperature in the laboratory (about 26ÂºC) for overnight, prepared for storage in two ways: -using and, -without silica gel, then stored in three storage temperatures, -5ÂºC, 5ÂºC and room temperature. Pollen germination was observed one day before storage, one week, four or five weeks and 12 weeks of storage. At the beginning, M. acuminata ssp banksii had highest pollen germination percentage, followed respectively by varieties bantamensis, breviformis, malaccensis and zebrina. The pollen germination ranks for the five banana varieties were remaining constant up to 12 weeks of storage though pollen germination dynamics occurred week by week. Silica gel may be recommended to be used until 5 weeks of storage. Storage temperature of -5ÂºC and 5ÂºC may be recommended as better storage temperature compared to room temperature. No special pattern shown by pollen tube vigor, but subspecies banksii pollen stored without silica gel, at 5ÂºC and room temperature tends to form better pollen tube vigor than stored at -5ÂºC.

TOLERANSI BEBERAPA GENOTIPE Vigna umbellata (THUMB.) TERHADAP SUHU TINGGI PADA BERBAGAI TAHAP PERTUMBUHAN [Heat Tolerance of Some Vigna umbellata (Thumb.) genotypes at different Growth Phases] Poerba, Yuyu Suryasari; Syarif, Fauzia
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Degree of electrolyte leakage (EL) from leaf tissue after exposure to high temperature has been used as an indicator of heat tolerance. Inthe present study, EL was measured in an attempt to estimate heat tolerance of Viana umbellata (Thumb.) genotypes at three differentgrowth phases. The degree of heat injury is significantly different among the three growth phases and among the genotypes as well as their interaction. However, the degree of heat injury tends to increase over the plant growth. The 18 genotypes of V. umbellata shows moderate genetic variability as shown by its value of genetic variance (46,25 ÃÂ±16,35), phenotypic variance (85,37 ÃÂ±27,60), and coefficientof genetic variation (26,36%).

KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN [Characterization of tetraploid Pisang Rejang induced by oryzalin] Poerba, Yuyu Suryasari; Handayani, T; Witjaksono, Witjaksono
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar. Â The Â triploid cultivar can be produced by crossing tetraploid with diploid cultivar. Â However, tetraploid banana cultivar is rarely existed naturally. Â Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed. Â Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer. Â Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 â 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit. Â The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.

ANALISA KESTABILAN GENETIK PISANG KEPOK âUNTI SAYANGâ HASIL MIKROPROGASI DENGAN MARKA RAPD DAN ISSR [Genetic Stability Analyses of Micropropagated Pisang Kepok âUnti Sayangâ by RAPD and ISSR Markers] Poerba, Yuyu Suryasari; Imelda, Maria; Martanti, Diyah
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) markers were used to evaluate the genetic stability of micropropagated plants of âPisang Kepok Unti Sayangâ at various stage of in vitro sub-cultures and in vivo plant material.All RAPD and ISSR profiles from micropropagated plants were monomorphic and similar to those of field grown control plants until stage tenth of sub cultures (V1S10). No variation was detected within the micropropagated plants, except for C12 (V1S44),G7 (V1S48 ), I11 and I12 (V1S10).RAPD and ISSR marker were both could be used to test the genetic stability of micropropagated bananas using the developed protocol.

ANALISA GENETIK PISANG HIBRID DIPLOID BERDASARKAN MARKA RAPD (Random Amplified Polymorphic DNA) [Genetic Analysis of Diploid Banana Hybrids Based on RAPD Markers] Martanti, Diyah; Poerba, Yuyu Suryasari; Herlina, Herlina
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Banana breeding by crosses is one way to get new cultivars with a better quality. This study aims to verify molecular characteristic of diploid hibrid bananas i.e MDRK, SNMT and MDMT using Random Amplified Polymorphic DNA (RAPD) markers. Ten RAPD primers have been selected to detect the differences in molecular pattern from all three diploid hybrid bananas. The results showed that the hybrid combination of MDRK, SNMT and MDMT produced bands that shared some similarities with each parents by 43.83%, 51.25% and 47.21% respectively as the number of bands from type I, III and IV markers. Type III marker is the important marker for identification of male parents. Type VII marker (non-parent bands) was detected from hybrid MDRK, SNMT and MDMT by 18 (20.22%), 6 (7.5%) and 11 (12.64%) respectively. Marker of type VII can be used to identified hybrid because they are present only in offspring. The highest polymorphic bands was obtained by SNMT (83.9%) and the lowest obtained was performed by MDMT (71.25%). From this result, RAPD markers can be used as a tool to detect the differences of molecular pattern of diploid hybrid bananas.

ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers] Poerba, Yuyu Suryasari; Ahmad, Fajarudin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.

STUDI KERAGAMAN GENETIK PULAI [Alstonia scholaris (L.) R.Br.] BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA Poerba, Yuyu Suryasari
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Alstonia scholaris (L.) R.Br. is a popular timber and medicinal tree species in Indonesia.The species is valued for its quality light wood timber and for its medicinal properties.Information on its existing genetic potential is currently lacking. The present study was carried out to optimize PCR and to screen primers among accessions collected from different part of region in Indonesia using random amplified polymorphic DNA (RAPD) markers in order to suggest appropriate primer and PCR conditions used in A.scholaris. Results showed that 26 primers generated 575 scorable bands of which 524 (92 %) were polymorphic.Ã Fourteen highly polymorphic primers (100% polymorphic) are recorded from 48 primer used, i.e.OPA-2, OPA-03, OPA-05, OPA-06, OPA-10,OPA-12, OPA-15, OPA-18, OPA-19, OPC-03, OPC-10, OPC-12, OPC-17, and OPN-14. Based on the RAPD markers, a dendrogram was constructed using the UPGMA method.The range of genetic distance was from 0.18-0.45.The molecular dara grouped the genotypes into three main clusters.

PENAMPILAN GENOTIPE SOM JAWA {Talinumpaniculatum Jacq. (Gaertn.)} PADA GERERASI M2 Poerba, Yuyu Suryasari
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Javasom [Talinum paniculatum Jacq. (Gaertn.] is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetable and as a substitute for purslane (Portulaca olaraeae L.).An effort to improve genetic quality of the plant was made through induced mutation with ethyl methane sulphonate (EMS).A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%, 1.2%, 1.5% and 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. A population of the first and second generation after mutagen treatment (M, and M2,respectively) was established and observed at vegetative and generative stages. At M, generation, plant growth was suppressed and plant yield was decreased.Chimeras and chlorophyll mutation were observed in every EMS-treatment level, which was indicated that mutation was induced in the plant. Lethal-dosage (LD-50) of EMS was at 1.2%-1.5%. M, population were recovered and showed variation in all parameters observed. High genetic variation coefficients were found in most of plant characters observed.All parameters have a medium to high heritability, which indicated that all parameter observed were relatively easily inherited.However, all genotypes are subjected to be evaluated in the next generation in their performance stability.

Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR Poerba, Yuyu Suryasari; Ahmad, Fajarudin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection.

Analisis Keragaman Genetik Dyera costulata (Miq) Hook.f. Berdasarkan Marka â€œRandom Amplified Polymorphic DNAâ€ Poerba, Yuyu Suryasari; Widjaya, Elizabeth A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Dyera costulata (Miq.) Hook.f (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia that occurs in Thailand, the Malay Peninsula and on the islands of Sumatera and Borneo. Its economic value was in its latex, used as gum chile in the manifacture of chewing gum. Nowadays the timber of this species is largely utilized for the manufacture of pencils and picture frames. The information on genetic diversity of the species is very limited. Hence studies were initiated and genetic diversity were estimated using Random Amplified Polymorphic (RAPD) markers in 47 accessions of Dyera costulata procured from different geographical regions of Jambi. Four selected Operon primers (10 mer) generated a total of 90 consistent amplification products ranging from 150 bp to 2.8 Kb. The cluster analysis showed that the 47 individuals were separated into one main cluster and one individual. The range of genetic dissimilarity value among samples was from 0.06 to 0.71, while genetic distance among populations was from 0.17 to 0.42. These values showed that those 47 accessions of D. costulata from Jambi was genetically originated from diverse population.

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