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All Journal EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Zulvia Intan Sari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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ISOLASI DAN KARAKTERISASI Beauveria bassiana SEBAGAI FUNGI ANTI HAMA Eka Sari; Zulvia Intan Sari; Anggi Nico Flatian; Eman Sulaeman
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 3 No 1 (2018): Ekotonia: Journal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.902 KB) | DOI: 10.33019/ekotonia.v3i1.755

Abstract

Development of biological potential could to be a solution of pest problems and environmental damage by pesticides. One of the organisms that are currently often be used for biopesticides is the entomopathogenic fungi such as Beauveria brassiana. This study aim to isolate, characterize of pest-resistant fungi and apply it to some agricultural insect pests in vitro. Samples which used were planthoppers, aphids, grasshoppers and isolated fungi Beauveria bassiana from Biogen Laboratory. The research was conducted in Soil and Environmental Biotechnology Laboratory, Dramaga, Bogor. Isolation method with Insect Bait Methode. Isolation and characterization of pest-resistant fungi that are planthoppers and aphids show the result that the great possibilities are the Beauveria bassiana fungus, that is clearly visible from the obtained physical characteristics, the white and sealed hyphae and conidia round oval. In addition, the fungi which used is a pathogenic fungi on the pest of aphids, planthoppers, and grasshoppers.
ISOLASI DAN KARAKTERISASI Rhizobium DARI Glycine max L. DAN Mimosa pudica Linn. Eka Sari; Anggi Nico Flatian; Zulvia Intan Sari; Eman Sulaeman
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 3 No 2 (2018): Ekotonia: Journal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.108 KB) | DOI: 10.33019/ekotonia.v3i2.760

Abstract

N availability in Indonesia is still relatively low. Various types of fertilization techniques are developed in order to reduce N losses, but the efficiency of N fertilizer use is not optimal. Therefore, biological N-belay technology is needed through Rhizobium inoculation to improve N fertilization in legume plants. This study aims to isolate and characterize Rhizobium bacteria from nodules formed in the roots of legume plants. The collection of Glycine max L. and Mimosa pudica Linn. root nodules is ashamed to be chosen from the pink root nodules. Rhizobium isolation using selective media of Yeast Extract Manitol Agar + congo red using the spread plate method. Characterization carried out, in the form of macroscopic observations, Gram staining, biochemical physiological tests, manufacturing growth curves and pathogenicity tests. The results of isolation from both plants obtained five types of Rhizobium isolates. The results of the characterization of isolates in both plants also showed the same thing, namely: bacteria have Echinulate shape when grown on sloping media, and when grown in cup media (large size, milky white, opaque (not penetrated by light, circular shape, convex elevation (convex), the surface is smooth shiny, the entire margin), including Gram negative bacteria, rod shaped, facultative anaerobic, motile, can degrade hydrogen peroxide, show positive oxidase, can ferment glucose and sucrose, grow well at pH 5 - pH 7, and not pathogenic to plants.
Co-Authors Anggi Nico Flatian EKA SARI Eman Sulaeman