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PENGARUH MEDIUM AIR CUCIAN BERAS TERHADAP KECEPATAN PERTUMBUHAN MISELIUM BIAKAN MURNI JAMUR TIRAM PUTIH Handiyanto, Sugeng; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Biakan murni Jamur tiram putih(Pleurotus ostreatus var. florida) merupakan pembibitan tahap pertama yang berperan sangat penting dalam budidaya jamur tiram. Pertumbuhan miselium biakan murni jamur tiram putih membutuhkan karbohidrat, protein, mineral dan vitamin. Air cucian beras berpotensi sebagai medium biakan jamur tiram putih karena mengandung karbohidrat, protein, mineral, dan vitamin yang dibutuh-kan dalam pertumbuhan jamur tiram putih. Penelitian ini bertujuan untuk: 1) menguji pengaruh medium air cucian beras dalam beberapa konsentrasi terhadap kecepatan pertumbuhan miselium jamur tiram putih; 2) menentukan konsentrasi medium air cucian beras terbaik yang menghasilkan kecepatan pertumbuhan miselium paling tinggi. Penelitian ini dilakukan di laboratorium Mikrobiologi, jurusan Biologi FMIPA Universitas Negeri Malang pada bulan Februari-April 2013. Jenis penelitian ini ialah penelitian eksperimental. Rancangan yang digunakan ialah Rancangan Acak Lengkap (RAL) dengan perlakuan macam konsentrasi air cucian beras 0% (sebagai kontrol negatif), 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, dan medium Potato Dekstrose Agar (sebagai kontrol positif) yang diulang sebanyak tiga kali. Pengukuran diameter koloni dilakukan pada hari ke-4, hari ke-7 dan hari ke-10 setelah inokulasi. Diameter koloni diukur berdasarkan rerata diameter koloni dengan arah horisontal dan vertikal. Kecepatan pertumbuhan miselium diperoleh dengan cara menghitung selisih diameter akhir dengan diameter awal kemudian dibagi rentang hari.Data kecepatan pertumbuhan miselium dianalisis dengan menggunakan Analisis Varians Tunggal 5% dan dilanjutkan dengan uji Beda Nyata Terkecil (BNT) 5%.Hasil penelitian ini menunjukkan bahwa: 1) terdapat perbedaan pengaruhmacam konsentrasi medium air cucian beras terhadap kecepatan pertumbuhan miselium jamur tiram putih; 2) konsentrasi medium air cucian beras terbaik ialah konsentrasi 90% yang menghasilkan kecepatan pertumbuhan miselium jamur tiram putih paling tinggi. Perlakuan kontrol positif dengan medium PDA menghasilkan diameter koloni lebih kecil dibandingkan diameter koloni pada perlakuan medium air cucian beras konsentrasi 90%. Kata Kunci:air cucian beras, kecepatan pertumbuhan, biakan murni, jamur tiram

Identifikasi Uji Kemampuan Hidrolisis Lemak Dan Penentuan Indeks Zona Bening Asam Laktat Pada Bakteri Dalam Wadi Makanan Traditional Kalimantan Tengah Rizky, Mirza Yanuar; Fitri, Rizka Diah; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
bionature Vol 18, No 2 (2017): Oktober
Publisher : Fakultas MIPA UNM

Abstract. Wadi is a sort of fermented fish product from Dayak tribe in Central Kalimantan. Wadi is made with the addition of lumu, sugar, and salt, and fermented for 7-10 x 24 hours. During the fermentation process, there are microflora from fish, and microflora from lumu that play a role in fermentation process. Some species of bacteria that play a role in the wadi fermentation process are lipolytic bacteria and Lactic Acid Bacteria (LAB). This research aims to: (1) identify the species of lipolytic bacteria and LAB that present in the wadi, (2) determine the lipid hydrolysis index and lactic acid clearance zone index of lipolytic bacteria and LAB that present in the wadi, and (3) determine the species of lipolytic bacteria and LAB which have highest ability to hydrolyze lipid and have the highest ability to produce lactic acid based on lipid hydrolysis index and lactic acid clearance zone index. The results showed that, (1) there are four species of lipolytic bacteria and LAB present in wadi: Lactobacillus coryniformis, Lactobacillus casei, Nitrococcus mobilis and Streptococcus lactis; (2) the four species of bacteria haveÂ different lipid hydrolysis index and lactic acid clearance zone index which are Lactobacillus coryniformis has a lipid hydrolysis index of 1.63 and lactic acid clearance zone index of 3.31; Lactobacillus casei has index of 1.94 and 3.96; Streptococcus lactis has index of 1.51 and 1.54 and Nitrococcus mobilis has index of 0.98 and 1.52; (3) Lactobacillus casei is a species of lipolytic bacteria and LAB which has the highest ability to hydrolyze lipid and to produce the highest lactic acid based on the lipid hydrolysis index and lactic acid clearance zone index, which are 1.94 and 3.96. Keywords: lipolitic bacteria, LAB, lipid hydrolysis index, lactic acid clearance zone, wadi.

Identification of Potential Bacteria on Several Lakes in East Java, Indonesia Based on 16S rRNA Sequence Analysis Achmad Rodiansyah; Ainul Fitria Mahmudah; Mastika Marisahani Ulfah; Uun Rohmawati; Dwi Listyorini; Eko Agus Suyono; Sitoresmi Prabaningtyas
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.136

Four bacterial isolates from Ranu Pani and Ranu Grati in east java had been revealed to be potentials to produce IAA (PIS isolate), phosphate solubilizer (GPS isolate), cellulose hydrolysis (PSS isolate) and, amylum hydrolysis (PAS), two dominant bacterial isolates from Rani Pani (PØD isolate) and Ranu Grati (GØD isolate) which were co-cultured with microalgae promoted microalgae growth, yet its taxonomical position has not been clearly known. The aim of this study was to identify those bacterial isolates using 16S rRNA barcode. This research conducted by gDNA isolation, the 16S rRNA sequence was amplified using 27F and 1492R primers. Reconstructed phylogenetic trees and genetic distance analysis showed that the isolate PIS and PSS identified as Bacillus cereus Group closely related to Bacillus paramycoides. PAS isolate identified as Bacillus subtilis Group closely related to Bacillus amyloliquefaciens, GPS isolate identified as novel species in genus Enterobacter, and two dominant isolates (PØD and GØD) identified as Enterobacter cloacae complex closely related to Enterobacter cloacae. The genomic approach and additional phenotypes-examination are required to clarify its taxonomical position.

Preliminary Study on Antibacterial Activity of Sawo Kecik (Manilkara kauki (L.) Dubard) Roots Extract Novida Pratiwi; Rini Retnosari; Sitoresmi Prabaningtyas
Jurnal Biodjati Vol 6, No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.7708

The roots of sawo kecik (Manilkara kauki (L.) Dubard) contain astringent that can be used to treat diarrhea for infants. However, the active antibacterial substance in sawo kecik roots has not been known. Therefore, the research to understand the antimicrobial activity of sawo kecik roots extract against Escherichia coli and Staphylococcus aureus was performed. Sawo kecik roots were macerated using methanol and chloroform. The result was then treated with E.coli and S.aureus with a concentration of 5 ppm and compared to negative control (solvent) and further observed and analyzed how reduced the bacterial growth with Two Ways ANOVA without interaction. The research results indicated that the sawo kecik roots methanol extract was effective to slow down the growth of S.aureus (0.160 ± 0.007) but ineffective against E.coli, whereas sawo kecik roots chloroform extract was not effective to reduce both bacteria. It can be concluded that further research is required to measure and verify the antibacterial activity of the extract using higher concentration samples and different research methodology.

PEMBERIAN PELATIHAN MEMBUAT BATIK JUMPUTAN KEPADA IBU PKK UNTUK UPAYA PELESTARIAN DAN MENINGKATKAN EKONOMI MASYARAKAT DI LOWOKWARU, MALANG Agung Witjoro; Murni Sapta Sari; Sri Rahayu Lestari; Mimien Henie Irawati; Susriati Mahanal; Fatchur Rohman; Sitoresmi Prabaningtyas; Siti Imroatul Maslikah
Jurnal KARINOV Vol 2, No 2 (2019): Mei
Publisher : Institute for Research and Community Service (LP2M), Universitas Negeri Malang

Batik merupakan warisan budaya khas Indonesia yang memiliki daya tarik tinggi di kawasan lokal maupun internasional. Batik jumputan adalah salah satu jenis batik yang mudah dibuat, memiliki banyak variasi teknik pembuatan, dan daya jualnya tinggi. Malang sebagai salah satu daerah tujuan wisata di Indonesia memiliki kesempatan besar untuk memanfaatkan batik jumputan menjadi komoditas di pusat oleh-oleh. Pemberdayaan masyarakat dalam pembuatan batik jumputan diharapkan dapat meningkatkan ekonomi masyarakat Malang, khusunya di daerah Lowokwaru. Upaya pelestarian melalui pelatihan perlu dilakukan untuk memberikan keterampilan membuat batik jumputan. Penelitian dan pengabdian masyarakat ini menggunakan metode deskriptif kualitatif. Teknik pengambilan data meliputi observasi, dokumentasi, dan survei. Partisipan dalam penelitian ini adalah ibu PKK di Lowokwaru, Malang. Analisis data dilakukan dengan mengelompokkan data hasil observasi dan kajian literatur ke dalam domain-domain hingga diperoleh suatu kesimpulan. Setelah melalui pelatihan, ibu PKK di Lowokwaru memiliki keterampilan untuk membuat batik jumputan dengan teknik ikat dan jahit untuk meningkatkan ekonomi masyarakat. Sehingga diharapkan dengan demikian batik jumputan dapat terus dilestarikan.

Isolation and characterization of α ‐amylase encoding gene in Bacillus amyloliquefaciens PAS Achmad Rodiansyah; Sitoresmi Prabaningtyas; Mastika Marisahani Ulfah; Ainul Fitria Mahmuda; Uun Rohmawati
Indonesian Journal of Biotechnology Vol 26, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.61425

Amylolytic bacteria are a source of amylase, which is an essential enzyme to support microalgae growth in the bioreactor for microalgae culture. In a previous study, the highest bacterial isolate to hydrolyze amylum (namely PAS) was successfully isolated from Ranu Pani, Indonesia, and it was identified as Bacillus amyloliquefaciens. That bacterial isolate (B. amyloliquefaciens PAS) also has been proven to accelerate Chlorella vulgaris growth in the mini bioreactor. This study aims to detect, isolate, and characterize the PAS’s α‐amylase encoding gene. This study was conducted with DNA extraction, amplification of α‐amylase gene with polymerase chain reaction (PCR) method with the specific primers, DNA sequencing, phylogenetic tree construction, and protein modeling. The result showed that α‐amylase was successfully detected in PAS bacterial isolate. The α‐amylase DNA fragment was obtained 1,468 bp and that translated sequence has an identity of about 98.3% compared to the B. amylolyquefaciens α‐amylase 3BH4 in the Protein Data Bank (PDB). The predicted 3D protein model of the PAS’s α‐amylase encoding gene has amino acid variations that predicted affect the protein’s structure in the small region. This research will be useful for further research to produce recombinant α‐amylase.

Utilization of a submersible pump as a plankton sampler for low-cost ecology practicum Dhiyauddin Aridhowi; Sitoresmi Prabaningtyas; Rina Tri Turani Saptawati; Diah Ayu Eka Fitriana; Yuslinda Annisa
Jurnal Teknologi dan Manajemen Pengelolaan Laboratorium Vol 4 No 1 (2021): Jurnal Teknologi Dan Manajemen Pengelolaan Laboratorium (Temapela)
Publisher : Labor Dasar dan Sentral Universitas Andalas

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The Van Dorn Bottle-water sampler which was operated during the plankton sampling practicum at the Department of Biology, FMIPA, State University of Malang experienced various obstacles, such as the tool being too heavy to use, difficulty opening and pouring water into the bottle, and the water sample bottle cap prone to falling and lost. These constraints affect the sampling of plankton that is less than optimal. Modification and use of the submersible pump is expected to replace the Van Dorn Bottle-water sampler to maximize plankton sampling. The submersible water collection pump is designed using five basic components, namely: a submersible pump, elastic-plastic hose, flow meter, timer, and battery power source, and can be used for various types of waters. Submersible pump trials were carried out at Ranu Grati lake, Pasuruan, East Java. The test results can be seen that the operation of the water pump is 5.9 kg lighter, can take more samples than the van dorn bottle-water sampler by 19.6 times and can take samples 35 seconds faster in each replication. With these advantages, the submersible pump can be used in plankton sampling practicum activities.

PENGARUH MEDIUM AIR CUCIAN BERAS TERHADAP KECEPATAN PERTUMBUHAN MISELIUM BIAKAN MURNI JAMUR TIRAM PUTIH Sugeng Handiyanto; Utami Sri Hastuti; Sitoresmi Prabaningtyas
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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Biakan murni Jamur tiram putih(Pleurotus ostreatus var. florida) merupakan pembibitan tahap pertama yang berperan sangat penting dalam budidaya jamur tiram. Pertumbuhan miselium biakan murni jamur tiram putih membutuhkan karbohidrat, protein, mineral dan vitamin. Air cucian beras berpotensi sebagai medium biakan jamur tiram putih karena mengandung karbohidrat, protein, mineral, dan vitamin yang dibutuh-kan dalam pertumbuhan jamur tiram putih. Penelitian ini bertujuan untuk: 1) menguji pengaruh medium air cucian beras dalam beberapa konsentrasi terhadap kecepatan pertumbuhan miselium jamur tiram putih; 2) menentukan konsentrasi medium air cucian beras terbaik yang menghasilkan kecepatan pertumbuhan miselium paling tinggi. Penelitian ini dilakukan di laboratorium Mikrobiologi, jurusan Biologi FMIPA Universitas Negeri Malang pada bulan Februari-April 2013. Jenis penelitian ini ialah penelitian eksperimental. Rancangan yang digunakan ialah Rancangan Acak Lengkap (RAL) dengan perlakuan macam konsentrasi air cucian beras 0% (sebagai kontrol negatif), 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, dan medium Potato Dekstrose Agar (sebagai kontrol positif) yang diulang sebanyak tiga kali. Pengukuran diameter koloni dilakukan pada hari ke-4, hari ke-7 dan hari ke-10 setelah inokulasi. Diameter koloni diukur berdasarkan rerata diameter koloni dengan arah horisontal dan vertikal. Kecepatan pertumbuhan miselium diperoleh dengan cara menghitung selisih diameter akhir dengan diameter awal kemudian dibagi rentang hari.Data kecepatan pertumbuhan miselium dianalisis dengan menggunakan Analisis Varians Tunggal 5% dan dilanjutkan dengan uji Beda Nyata Terkecil (BNT) 5%.Hasil penelitian ini menunjukkan bahwa: 1) terdapat perbedaan pengaruhmacam konsentrasi medium air cucian beras terhadap kecepatan pertumbuhan miselium jamur tiram putih; 2) konsentrasi medium air cucian beras terbaik ialah konsentrasi 90% yang menghasilkan kecepatan pertumbuhan miselium jamur tiram putih paling tinggi. Perlakuan kontrol positif dengan medium PDA menghasilkan diameter koloni lebih kecil dibandingkan diameter koloni pada perlakuan medium air cucian beras konsentrasi 90%. Kata Kunci:air cucian beras, kecepatan pertumbuhan, biakan murni, jamur tiram

Improving Community Skills in Making Batik Jumputan Through Training PKK Mothers at Sumbergempol, Kabupaten Tulungagung Siti Imroatul Maslikah; Murni Saptasari; Sitoresmi Prabaningtyas; Srirahayu Lestari; Agung Witjoro; Mimien Henie Irawati; Susriyati Mahanal; Dyah Ayu Eka Fitriana; Muhammad Athoillah; Yuslinda Anisa; Siti Nur Arifah; Alif Rofiqotun Nurul Alimah
Berdikari: Jurnal Pengabdian Masyarakat Indonesia Vol. 2 No. 1 (2019): Berdikari: jurnal Pengabdian Masyarakat Indonesia
Publisher : Future Science

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The purpose of this study is to improve the community’ skills of making batik jumputan through training of 21 PKK mothers at Sumbergempol District, Tulungagung Regency. This type of research is descriptive qualitative. Participants involved in this study were selected randomly (random sampling technique). Data collection techniques in the form of skills test making batik jumputan (paper based-test) conducted before and after training. Data analysis was performed using the calculation and categorization of N-Gain. The results showed that the training in making batik jumputan could improve the skills of PKK mothers at Sumbergempol District, Tulungagung Regency in the moderate (62%) and high (38%) categories.

Identifikasi Jenis Kapang Kontaminan Kedelai (Glycine max (L.) Merr.) di Pasar Tradisional Kota Malang Fitria Maulita; Utami Sri Hastuti; Sitoresmi Prabaningtyas
Jurnal Mikologi Indonesia Vol 5, No 1 (2021): Juni 2021
Publisher : Perhimpunan Mikologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46638/jmi.v5i1.139

Biji kedelai dapat mengalami kerusakan, antara lain: karena pemanenan yang kurang cermat, serangan serangga hama di lahan pertanian, gigitan serangga di tempat penyimpanan biji sebelum dijual, serta terkontaminasi oleh kapang penghasil mikotoksin di pasar. Fokus dari penelitian ini ialah: 1) mengisolasi dan mengidentifikasi semua jenis kapang kontaminan pada biji kedelai yang dijual di beberapa pasar di Kota Malang, 2) menentukan jenis kapang kontaminan yang paling dominan dalam biji kedelai yang diteliti, dan 3) mengkaji dampak cemaran kapang kontaminan terhadap penurunan kualitas biji kedelai berdasarkan kajian pustaka. Data diperoleh melalui metode observasi, deskripsi, dan identifikasi semua jenis kapang kontaminan yang ditemukan pada biji kedelai yang diteliti. Selanjutnya dilakukan identifikasi dengan deskripsi morfologi koloni dan mikroskopis kapang. Hasil yang diperoleh yaitu tujuh isolat jenis kapang kontaminan, yakni Scopulariopsis brevicaulis, Penicillium frequentans, Aspergillus tamarii, mycelia sterilia I, Aspergillus oryzae, Aspergillus ochraceus, dan mycelia sterilia II; 2) jenis kapang kontaminan yang paling dominan yaitu Aspergillus ochraceus; 3) cemaran kapang berdampak terhadap penurunan kualitas biji kedelai, yaitu: tekstur biji berserbuk, hancur, keriput, berbau apak, dan berpotensi tercemar oleh mikotoksin yang dihasilkan kapang kontaminan.

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