Garuda - Garba Rujukan Digital

ANALISIS PRODUK DARAH: KONTAMINASI BAKTERI PADA THROMBOCYTE CONCENTRATE Btari Christiyani Kusumaningrum, Serafica; Sepvianti, Wiwit
MEDIA ILMU KESEHATAN Vol 7 No 3 (2018): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Background: Bacterial contamination in thrombocyte concentrate (TC) blood product becomes a serious problem since it can cause clinical risks from mild symptom to death. TC storage condition at 22-25oC with agitation is good condition for bacterial growth. Source of bacterial contamination can come from donor bacteremia, not aseptically from taking and processing blood product. In a previous study, showed there were positive gram bacteria as bacterial contamination in TC. Objective: This objective aims to determine bacterial contaminants in TC blood products. Methods: Total of 3 TC bag samples were collected from UDD PMI Kabupaten Sleman. Each sample was inoculated to nutrient agar media and it incubated for 48 jam at 37oC. Observation of bacterial colony growth is carried out after incubation time is over. Results: There was no bacterial growth in the media so that there was no bacterial contamination in TC. It showed that the procedures, processing, and storage of TC products in PMI Kabupaten Sleman were aseptic. Besides that, TC donors also did not suffer from bacteremia so there was bacterial contamination in TC. Conclusion: In this study, bacterial contamination in blood product TC was not found since there was no bacterial growth in media. Keywords: Bacterial contamination, blood products, blood bank, thrombocyte concentrate.

GAMBARAN JUMLAH ERITROSIT PADA WHOLE BLOOD SELAMA 30 HARI PENYIMPANAN DI PMI KABUPATEN SLEMAN YOGYAKARTA Andriyani, Yuni; Btari C.K, Serafica; Sepvianti, Wiwit
Prosiding Conference on Research and Community Services Vol 1, No 1 (2019): Prosiding Conference on Research and Community Services)
Publisher : STKIP PGRI Jombang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Transfusi darah adalah proses pemberian darah atau komponen darah dari satu individu (donor) ke individu lainnya (resipien). Whole Blood (WB) adalah salah satu produkdarah yang dapat ditransfusikan dengan komponen yang masihlengkap mengandung eritrosit, leukosit, trombosit dan plasma. Indikasi pemberian WB yaitu untuk meningkatkan jumlah sel darah merah dan volume plasma dalam waktu yang bersamaan.WB dengan anti koagulan CPDA-1 (Citrate Phosphate Dextrose Adenine-1) disimpan pada suhu 2-6 C dengan lama penyimpanan sampai 35 hari. Masa penyimpanan WB akan menimbulkan efek berupa perubahan integritas membran eritrosit. Tujuan dari penelitian iniuntuk mengetahui jumlah eritrosit pada WB selama 30 hari penyimpanan. Jenis penelitian ini adalah observasional deskriptif dengan desain Cross Sectional. Jumlah sampel yang diteliti adalah 1 kantong darah WB dengan golongan darah O. Berdasarkan hasil pemeriksaan jumlah eritrosit pada WB menggunakan Hematology Analyzer Sysmex XS-800i selama 30 hari penyimpanan menunjukan penurunan jumlah eritrosit pada hari ke-0 yaitu 5,02 x 106/ L, hari ke-5 yaitu 5,00 x 106/ L, hari ke-10 yaitu 4,95 x 106/ L hari ke-15 yaitu 4,94 x 106/ L, hari ke-20 yaitu 4,91 x 106/ L, hari ke-25 yaitu 4,98 x 106/ L, dan hari ke-30 yaitu 4,73 x 106/ L. Pada Penelitian ini terjadi penurunan jumlah eritrosit sebesar 5,7 %. Dimana persen penurunan tersebut masih dalam batas normal yang artinya kualitas WB masih baik..

Identifikasi Bakteri Kontaminan Pada Produk Darah Thrombocyte Concentrate Kusumaningrum, Serafica Btari Christiyani; Sepvianti, Wiwit
Syifa'Medika Vol 10, No 2 (2020): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v10i2.2185

Saat ini kontaminasi bakteri pada produk darah masih menjadi permasalahan serius karena memiliki resiko transfusi yang fatal. Kasus kontaminasi bakteri pada TC masih banyak terjadi karena kondisi penyimpanan TC yang sesuai dengan pertumbuhan bakteri. Sumber kontaminasi bakteri diperoleh dari proses pengambilan dan pengolahan darah yang kurang aseptis. Penelitian ini bertujuan untuk mengidentifikasi bakteri kontaminan pada TC. Sampel yang digunakan merupakan kultur TC yang telah dinyatakan positif mengandung bakteri menggunakan Bact/Alert dan diperoleh dari UTD PMI Kota Yogyakarta. Sampel disubkultur pada media blood agar dan diinkubasi selama 48 jam pada suhu 37oC. Purifikasi dilakukan pada isolat bakteri yang diperoleh. Hasil identifikasi ditentukan berdasarkan bentuk sel, bentuk koloni dan sifat biokimia isolat bakteri. Berdarkan hasil yang diperoleh terdapat dua isolat bakteri (TC1 dan TC2) yang berhasil diisolasi dan dilakukan karakterisasi berdasarkan bentuk sel, sifat biokimia dan bentuk pertumbuhan. Hasil identifikasi menunjukkan bahwa isolat TC1 merupakan Staphylococcus epidermidis dan TC2 merupakan Bacillus sp. Pada penelitian ini didapatkan isolat bakteri Staphylococcus epidermidis dan Bacillus sp. yang merupakan bakteri kontaminan produk drahthrombocyte concentrate.

Sintesis Dan Uji Aktivitas Antibakteri Senyawa Kalkon (E)-3-(4-(Dimethylamino)Phenyl)-1-Phenylprop-2-En-1-One Terhadap Bakteri Kontaminan Produk Darah Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Journal of Health (JoH) Vol 8 No 2 (2021): Journal of Health - Juli 2021
Publisher : LPPM STIKES Guna Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30590/joh.v8n2.p75-84.2021

Chalcone compounds and their derivatives are known to have various pharmacological activities, one of which is antibacterial activity. This activity is strongly influenced by the type of bound substituent and the location of the substituent on the structure of the chalcone compound. The purpose of this study was to synthesize chalcone compounds with the characteristics of the dimethylamino substituent on ring B at the para ((E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one) position and to test the activity of an antibacterial compound of chalcone against bacterial isolates of blood product contaminants. Antibacterial activity testing was focused on bacterial isolates derived from blood products, because the use of blood product bacterial isolates for antibacterial testing was still not widely used, generally the test bacteria used were ATCC bacterial isolates. The antibacterial activity test method used was the paper disc diffusion method, the antibacterial activity of chalcone compounds was known through the formation of a clear zone around the disc paper called the inhibition zone for bacterial growth. The chalcone compound (E)-3-(4-(dimethylamino)phenyl)-1-phenylprop-2-en-1-one was successfully synthesized through a condensation reaction, which obtained a yellow crystalline solid with a yield of 81.67%. The results of the activity test of chalcone compounds on several blood product contaminants showed that this compound had good enough activity in inhibiting the growth of Staphylococcus aureus bacteria with an inhibition zone of 8.9 mm at a concentration of 25 ppm.

Pengaruh Frekuensi Mengkonsumsi Kopi Dengan Kadar Hemoglobin Calon Pendonor Laki-Laki Di PMI Kabupaten Sleman Relita Pebrina; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; RIfail
Jurnal Ilmu Kesehatan MAKIA Vol 11 No 2 (2021): Jurnal Ilmu Kesehatan MAKIA
Publisher : LPPM STIKes ICsada Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37413/jmakia.v11i2.173

ABSTRAK Donor darah adalah proses pengambilan darah dari seseorang secara sukarela untuk disimpan di bank darah yang kemudian digunakan untuk keperluan transfusi darah. Sebelum pelaksanaan pengambilan darah (aftap), calon donor terlebih dahulu diseleksi untuk mengetahui apakah memenuhi syarat/ kriteria yang ditentukan. Seleksi donor diantaranya adalah pemeriksaan kadar hemoglobin (Hb). Kadar Hb setiap orang berbeda-beda, hal ini dapat dipengaruhi oleh beberapa faktor salah satunya minuman yang populer saat ini adalah kopi. Tujuan penelitian ini adalah mengetahui pengaruh frekuensi minum kopi dengan kadar hemoglobin calon pendonor laki-laki. Jenis penelitian ini bersifat deskriptif korelational dengan pendekatan cross sectional. Sampel penelitian berjumlah 50 responden ditentukan dengan concecutive sampling. Hasil yang didapat dalam penelitian ini yaitu frekunesi mengkonsumsi kopi responden 4 kali dalam seminggu memiliki kadar Hb normal 40,0% lebih banyak dari kadar Hb rendah 2,0% dan kadar Hb tinggi 0,0%, sedangkan responden dengan perilaku >4 kali minum kopi dalam seminggu memiliki kadar Hb normal 30,0% lebih banyak dari kadar Hb rendah 4,0% dan kadar Hb tinggi 0,0%. Responden dengan perilaku 6 kali minum kopi dalam seminggu memiliki kadar Hb normal 20,0% lebih banyak dari kadar Hb rendah 4,0% dan kadar Hb tinggi 0,0%. Kesimpulan penelitian ini adalah terdapat pengaruh frekuensi konsumsi kopi dengan kadar hemoglobin calon pendonor laki-laki dengan nilai signifikasi 0.000.

ANTIBACTERIAL ASSAY OF 2’-HYDROXY-4’,6’,4-TRIMETHOXYCHALCONE AND 4-METHOXYCHALCONE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Jurnal Riset Kesehatan Vol 8, No 2 (2019): NOVEMBER 2019
Publisher : Poltekkes Kemenkes Semarang

Blood transfusion is currently one of the most important health care treatments. Unfortunately, the prevalence of transfusion reactions caused by the use of blood products is still quite high although blood products have been tested in terms of infectious diseases using 4 parameters, namely hepatitis B, hepatitis C, HIV and syphilis. The results of the analysis of blood products produced by UTD (Blood Transfusion Unit) Surabaya showed bacterial contamination of blood products, starting from normal skin flora to pathogens. Bacterial contamination is also believed to be one of the causes of transfusion reactions. Therefore, it is necessary to make efforts to prevent bacterial contamination of blood products. One of the efforts is to find chemical compounds that have good antibacterial activity. In this study, the antibacterial activity of 2ˈ-hydroxy-4ˈ,6ˈ,4-trimethoxychalcone and 4-methoxychalcone was tested against gram positive and gram negative bacteria. The test method was agar diffusion at various concentrations of chalcone, namely 0.625; 1.25; 2.5 and 5.0%. The results showed that both chalcones had good antibacterial activity against gram-positive and gram-negative bacteria.

Aktivitas Antibakteri Senyawa 2’-hidroksi-4’,6’ dimetoksikalkon Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Journal of Health (JoH) Vol 6 No 1 (2019): Journal of Health - Januari 2019
Publisher : LPPM STIKES Guna Bangsa

Kalkon merupakan senyawa flavanoid rantai terbuka yang memiliki beragam aktivitas farmakologis diantaranya sebagai antioksidan, antibakteri, antiinflamatori dan antikanker. Senyawa 2’-hidroksi-4’,6’-dimetoksikalkon telah dilaporkan memiliki aktivitas antikanker yang sangat baik dan bersifat selektif, yaitu hanya aktif mematikan sel kanker namun tidak mematikan sel normal. Aktivitas senyawa 2’-hidroksi-4’,6’-dimetoksikalkon yang baik terhadap sel kanker diharapkan juga bekerja pada bakteri. Penggunaan alkohol sebagai desinfektan pada proses aftap memberi resiko lisis darah, sehingga diperlukan senyawa kimia yang efektif sebagai antibakteri namun minim resiko melisiskan darah. Aktivitas antibakteri senyawa kalkon diuji menggunakan metode difusi agar dengan sumuran, zona bening yang terbentuk disekitar sumuran menunjukan aktivitas senyawa kalkon dalam menghambat pertumbuhan bakteri. Pada penelitian ini seluruh larutan kalkon dalam variasi konsentrasi 2; 4; 8; 10; dan 20 % tidak menunjukkan aktivitas penghambatan pertumbuhan bakteri, sedangkan pada sumuran yang diisi padatan kristal kalkon menunjukkan zona bening sekitar 1 mm sehingga dapat disimpulkan bahwa senyawa 2’-hidroksi-4’,6’-dimetoksikalkon tidak memiliki aktivitas sebagai antibakteri.

Gambaran Kadar Hemoglobin pada Sediaan Produk Darah Packed Red Cells (PRC) selama Masa Simpan 20 hari Wiwit Sepvianti; Meyta Wulandari; Serafica Btari Christiyani Kusumaningrum; Sunartono Sunartono; Taib Djafar
Journal of Health (JoH) Vol 6 No 2 (2019): Journal of Health - July 2019
Publisher : LPPM STIKES Guna Bangsa

Packed Red cells (PRC) merupakan sediaan produk darah yang tercacat paling banyak digunakan pada transfusi darah. Indikasi penggunaan PRC adalah untuk menaikan kadar hemoglobin pada pasein. 1 unit PRC dapat menaikkan kadar hematokrit 3-5 %. Peran vital pemberian PRC dalam menaikkan kadar hemoglobin tidak terlepas dari fakta bahwa PRC terus mengalami penurunan kualitas selama masa penyimpanan. Hal ini disebabkan sel-sel darah mengalami lisis, sehingga berpengaruh secara langsung pada morfologi sel, kadar hemoglobin, pH darah, dan kadar ion-ion darah. Pada penelitian ini dilakukan pengamatan kadar Hemoglobin pada sediaan PRC selama masa simpan 20 hari menggunakan metode Rapid test. Sampel penelitian sejumlah 3 kantong darah PRC bergolongan darah O rhesus positif. Hasil pengamatan kadar Hemoglobin pada sampel PRC menunjukkan terjadi penurunan kadar PRC pada masa simpan 20 hari yang berkisar antara 7,8- 11,2%.

ANALISIS PRODUK DARAH: KONTAMINASI BAKTERI PADA THROMBOCYTE CONCENTRATE Serafica Btari Christiyani Kusumaningrum; Wiwit Sepvianti
MEDIA ILMU KESEHATAN Vol 7 No 3 (2018): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v7i3.247

Background: Bacterial contamination in thrombocyte concentrate (TC) blood product becomes a serious problem since it can cause clinical risks from mild symptom to death. TC storage condition at 22-25oC with agitation is good condition for bacterial growth. Source of bacterial contamination can come from donor bacteremia, not aseptically from taking and processing blood product. In a previous study, showed there were positive gram bacteria as bacterial contamination in TC. Objective: This objective aims to determine bacterial contaminants in TC blood products. Methods: Total of 3 TC bag samples were collected from UDD PMI Kabupaten Sleman. Each sample was inoculated to nutrient agar media and it incubated for 48 jam at 37oC. Observation of bacterial colony growth is carried out after incubation time is over. Results: There was no bacterial growth in the media so that there was no bacterial contamination in TC. It showed that the procedures, processing, and storage of TC products in PMI Kabupaten Sleman were aseptic. Besides that, TC donors also did not suffer from bacteremia so there was bacterial contamination in TC. Conclusion: In this study, bacterial contamination in blood product TC was not found since there was no bacterial growth in media. Keywords: Bacterial contamination, blood products, blood bank, thrombocyte concentrate.

Analog Kalkon (E)-1,3-bis(4-hydroxyphenyl)prop-2-en-1-one: Sintesis dan Uji Aktivitas Antimicrobia terhadap Bakteri Kontaminan Produk Darah Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Jurnal Kesehatan Saelmakers PERDANA (JKSP) Vol. 4 No. 2 (2021): Jurnal Kesehatan Saelmakers PERDANA
Publisher : Fakultas Ilmu Kesehatan Universitas Katolik Musi Charitas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32524/jksp.v4i2.287

Bacterial infection remains a leading cause of death in the world. It caused by incorrect antimicrobial used in the hospital, bacterial resistance caused by prolonged use of antimicrobial, minimum controlling of antimicrobial utilization, and late development of new antimicrobial drugs. Chalcone compounds with particular structure are believed to have the capability as an antimicrobial agent. The objective of this study is to synthesize and examine the activity of chalcone analogue compound with two hydroxyl substituents groups in ring A and B as a characteristics. Chalcone analogue (E)-1,3-bis(4-hydroxyphenyl)prop-2-en-1-one was successfully synthesized by Claisen-Schmidt condensation method and produced a pale-yellow crystalline powder with 89.40% yield as reaction powder. The elucidation of reaction product structure using FTIR and GC-MS. Furthermore, the GC-MS test was obtained one-peak chromatogram with 100% of relative purity and m/z 240 as molecular ions detected according to the target compound of (E)-1,3-bis(4-hydroxyphenyl)-prop-2-en-1-one molecular weight. Antimicrobial activity examination was performed by paper disk diffusion method and the result showed that chalcone analog compound (E)-1,3-bis(4-hydroxyphenyl)prop-2-en-1-one has medium inhibition activity to Staphylococcus aureus as positive gram bacteria, with the clear zone diameter was 9,2 mm in the 25,00 ppm of concentration. This compound were indicated to work selectively into Staphylococcus aureus, because it was not actively inhibit the growth of another bacterial test such as Bacillus sp., Escherichia coli and Acinetobacter

Title

Found 25 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 25 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 25 Documents
Search