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Uji Lapang Pakan Bervaksin Aeromonas hydrophila pada Lele Dumbo di Daerah Banyumas Dini Siswani Mulia; Irma Tri Susanti; Heri Maryanto; Cahyono Purbomartono
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.28 KB)

Abstract

The main problem in the cultivation of African catfish is a disease MAS (Motile Aeromonas Septicemia) Aeromonas hydrophila bacteria attack. In addition, other problems that also resulted in the successful cultivation of this fish is the high price of fish feed and the cost of production for the procurement of the feed. Needs to be studied the use of feed raw materials that utilize industrial waste as well as the use of vaccines in controlling disease MAS. This study aims to apply feed made from 3 waste, namely chicken feathers, pulp, and trash fish, then add the vaccine (vaccine feed) and tested in the field. Field test conducted in the village of Bangsa, District of Kebasen, Banyumas. This study uses a completely randomized design (CRD) with 3 treatments and 5 replications. The treatment consisted of P1 = vaccine feed 10 days; P2 = vaccine feed 15 days, and P3 = no vaccine feed (controls). Feed was given as much as 5% of the weight of fish / day. Research using plastic tarp length x width x height = 80 x 60 x 60 cm. African catfish which used a length of 10-13 cm with a weight of 7.3 to 21.1 g. Fish reared for 8 weeks. Parameters measured were immune response such as antibody titer, weight gain and length, specific growth rate (SGR), as well as survival. Parameters measured were supporting water quality parameters include water temperature, pH, and dissolved oxygen. Data were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT test) with a test level of 5%. The results showed that the vaccine could increase the production of antibody titers (P <0.05) compared to controls. The use of artificial feed of chicken feathers, pulp, and trash fish are added the vaccine (vaccine feed) can be applied in the field.Keywords: Aeromonas hydrophila, Banyumas, field test, vaccine feed
IMUNOGENISITAS HEAT KILLED Aeromonas hydrophila Strain GB-01, GPd-02, dan GPl-05 SEBAGAI KANDIDAT VAKSIN Dini Siswani Mulia; Cintya Windarti; Heri Maryanto
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 17, No 2 (2016): Techno Volume 17 No 2, Oktober 2016
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/techno.v17i2.1176

Abstract

Abstrak. Vaksinasi Aeromonas hydrophila merupakan langkah konkrit untuk mengendalikan penyakit Motile Aeromonas Septicemia (MAS) yang disebabkan oleh bakteri A.hydrophila. Namun, diperlukan strain bakteri A. hydrophila yang memiliki imunogenisitas tinggi. Pembuatan vaksin dengan metode heat killed merupakan salah satu cara pembuatan vaksin inaktif A. hydrophila selain vaksin inaktif dengan formalin (whole cell). Penelitian bertujuan untuk mengkaji potensi imunogenisitas heat killed A.hydrophila strain GB-01, GPd-02, dan GPl-05 sebagai kandidat vaksin. Penelitian menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL), 3 perlakuan (strain GB-01, GPd-02, dan GPl-05). dan 1 kontrol dengan 4 kali ulangan. Vaksin dibuat dengan memanaskan bakteri pada suhu 100ºC selama 2 jam. Ikan uji adalah lele dumbo berumur sekitar 2 bulan dengan ukuran panjang 9-14 cm. Parameter penelitian meliputi titer antibodi, uji reaksi silang, dan parameter kualitas air, yaitu suhu air, pH, dan kadar oksigen terlarut. Data titer antibodi dianalisis dengan Analysis of Variance (Anova) dan Duncan’s Multiple Range Test (DMRT) pada taraf uji 5 %, sedangkan data hasil reaksi silang dan parameter kualitas air diamati secara deskriptif. Hasil penelitian menunjukkan bahwa strain GB-01, GPd-02, dan GPl-05 dapat meningkatkan titer antibodi dibandingkan kontrol dan bereaksi positif pada uji reaksi silang, sehingga disimpulkan memiliki imunogenisitas yang tinggi dan direkomendasikan menjadi kandidat vaksin. Kata Kunci : Aeromonas hydrophila, heat killed, imunogenisitas, kandidat vaksin, strain GB-01, GPd-02, dan GPl-05