Nova Pramestuti
Balai Penelitian dan Pengembangan Pengendalian Penyakit Bersumber Binatang Banjarnegara, Jl. Selamanik No. 16A, Kec. Banjarnegara, Jawa Tengah, Indonesia

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Leptospirosis: New Emerging Disease in Sukoharjo District Marbawati, Dewi; Pramestuti, Nova
KEMAS: Jurnal Kesehatan Masyarakat Vol 13, No 2 (2017)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v13i2.4849

Abstract

Leptospirosis in Sukoharjo was discovered in 2014. Examination with RDT (Rapid Diagnostic Test) leptotek supported by clinical symptoms showed 6 positive cases of Leptospira, and until March 2015 one person was found to be Leptospira positive. The aim of this study was to identify rats as the main reservoir of leptospirosis, calculate the catching rate of rats and to detect the presence of pathogenic Leptospira in rats. This study was a cross-sectional survey, and was conducted in Pabelan village Kartasura Sukoharjo on May 2015. Polymerase Chain Reaction (PCR) assay was conducted in Bacteriology Laboratory Balai Litbang P2B2 Banjarnegara to detect leptospira in the kidney of the rats. Data were analyzed descriptively. Results of rats and shrew catching obtained Rattus tanezumi, Rattus norvegicus and Suncus murinus. The species most commonly found was balanced between R. tanezumi and S.murinus (46%). The trap success rate inside and outside the house are 1.50% and 5%, respectively. Result of laboratory test showed from 13 rats kidneys, two kidneys were found to be Leptospira positive and was from R.tanezumi and R.norvegicus.
SPOT SURVEI LEPTOSPIROSIS DI KECAMATAN NGEMPLAK DAN NOGOSARI, KABUPATEN BOYOLALI, PROVINSI JAWA TENGAH Pramestuti, Nova; Ikawati, Bina; Widiastuti, Dyah
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 7 No 1 (2014): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.472 KB)

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ABSTRACTIn 2013 there were three leptospirosis cases reported in Boyolali. This study aimed to describe the epidemiology of leptospirosis including cases overview aboutperson, place and time, transmission history, the trap success in catching mice and rats, and species of Leptospira infected rodent in Ngemplak and Nogosari district, Boyolali. This study was a cross sectional study. Rodent traping conducted at three locations with leptospirosis cases in Ngemplak and Nogosari District, Boyolali, in April 2013. A total of 300 traps wereinstalled, 2 traps inside and 2 traps outside for each house during three days. The trapped mice and rats were identified, counted their population density and their kidneys were taken for Leptospira examainationusingPCR (Polymerase Chain Reaction) assay. Data were processed and analyzed descriptively, presented in a frequency distribution form. The result showed that transmission of leptospirosis may occured around the case? house, the river and rice fields. The highest rodentsuccess trapwas found in KismoyosoVillage (14 %). Rat species which were found consisted of R.tanezumi, and R. indica Bandicota argentiventer, there was no rat kidney samples infected with Leptospira bacteria according to PCR assay. Leptospirosis transmission in Boyolali should be noteddue to the high rat trap success and the location which was closed to the river . Key words: Survey,leptospirosis, rats Kesmasindo, Volume 7( 1 )Juli 2014, Hal 63-70
The Natural Rodenticidal Effect of Papain Papaya (Carica papaya L.) in Histopathological Changes of Albino Mice Organs Pramestuti, Nova; Sianturi, Corry Laura Junita; Lestari, Eva; Wijayanti, Siwi Pratama Mars
Kesmas Indonesia: Jurnal Ilmiah Kesehatan Masyarakat Vol 11 No 2 (2019): Jurnal Kesmas Indonesia
Publisher : Jurusan Kesehatan Masyarakat dan Fakultas Ilmu-Ilmu Kesehatan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (653.386 KB) | DOI: 10.20884/1.ki.2019.11.2.1636

Abstract

Reports on the rodenticide effect of papain papaya to control rats and mice is scarce. The aim of this study was to determine pathological changes in  albino mice internal organs (gaster, kidney, liver and intestine) associated with toxicity effect of papain papaya (Carica papaya L.). Seventy-eight adult BALB/c mice of both sexes were divided into 5 groups: 1 control group and 4 treatment groups that were given papain feeding block. Feed intake and mortalities have been monitored in previous study. At the end of the experimental period, tissues of mice were harvested for necropsy and histopathological examination. The abnormality of post mortem examination were hepatomegaly, gastro-intestinal bleeding. Dark discoloration was founded in liver and gaster. Histopathological changes founded in all treatment albino mice were necrosis in gaster, kidney, liver and intestine. Cloudy swelling occurred in liver and dilatation in kidney. Fatty degeneration occured in gaster, liver and intestine. In addition, the kidney and liver were congested. Feed intake of 16 to 32% papain natural rodenticide gave effect to the organs of studied mice, i.e gaster, kidney, liver and intestine.Reports on the rodenticide effect of papain papaya to control rats and mice is scarce. The aim of this study was to determine pathological changes in  albino mice internal organs (gaster, kidney, liver and intestine) associated with toxicity effect of papain papaya (Carica papaya L.). Seventy-eight adult BALB/c mice of both sexes were divided into 5 groups: 1 control group and 4 treatment groups that were given papain feeding block. Feed intake and mortalities have been monitored in previous study. At the end of the experimental period, tissues of mice were harvested for necropsy and histopathological examination. The abnormality of post mortem examination were hepatomegaly, gastro-intestinal bleeding. Dark discoloration was founded in liver and gaster. Histopathological changes founded in all treatment albino mice were necrosis in gaster, kidney, liver and intestine. Cloudy swelling occurred in liver and dilatation in kidney. Fatty degeneration occured in gaster, liver and intestine. In addition, the kidney and liver were congested. Feed intake of 16 to 32% papain natural rodenticide gave effect to the organs of studied mice, i.e gaster, kidney, liver and intestine.