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All Journal Indonesian Journal of Chemical Science Animal Production : Indonesian Journal of Animal Production
Edy Pramono
Civil Department
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Veterinary

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KAJIAN KESAHIHAN PERSAMAAN ESPENSON (1995) UNTUK REAKSI ENZIMATIS DAN YANG MIRIP Patiha, Patiha; Mudjijono, Mudjijono; Hidayat, Yuniawan; Pramono, Edy
Indonesian Journal of Chemical Science Vol 3 No 2 (2014)
Publisher : Indonesian Journal of Chemical Science

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Telah dilakukan kajian tentang kesahihan persamaan Espenson (1995). Pertama secara teoritis atas paradigma yang digunakan dan kemudian dari hasilpenerapannya pada penentuan tetapan Michaelis-Menten dari reaksi brominasi aseton.  Dikatakan sahih jika, secara teoritis konsisten dengan konsep dasar kinetika kimia dan reaksi enzimatisdan memberikan harga tetapan Michaelis-Menten yang pasti. Ditemukan bahwa persamaan tersebut tidak/kurang sahih.
The Effect of Level Testosteron e Addition in Diluents and Level Dilution on Speed Movement and Abnormality of Kedu Chicken Sperm Susanto, Agus; Wahyuningsih, Rachmawati; Pramono, Edy
ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The research entitled “The Effect of Level Testosterone Addition in Diluents and Level Dilution on Speed Movement and Abnormality of Kedu Chicken Sperm” was conducted in Laboratory of Physiology and Reproduction, Faculty of Animal Science, UNSOED, started at August 15th to September 15th , 2001. The aims of  this research were to obtain influences of level testosterone and dilution on speed movement and secondary abnormality kedu chicken sperm and obtain interaction between the treatments. The tapped sperm from nine kedu chickens were used in this research. This Experiment was performed 4 x 3 Factorials with Randomized  Completely Block Design (RCBD) as the basic design. The treatment combinations were level testosterone 0, 300, 600 and 900 μg (t0, t1, t2 and t3) and level of dilution 4, 6 and 8 time (in a row p1, p2 and p3). The tapping period was replicated four times as a group (replicated) with two days interval. The result of this research showed that the interaction between level of testosterone addition and level of dilution gave a non-significant effect to speed movement sperm but significant to abnormality of kedu chicken sperm. The group gave a significant influence (P<0.05) to speed movement sperm and non significant to sperm abnormality. The interaction of level testosterone addition and level dilution of kedu chicken semen (T x P) has a quadratic regression to sperm abnormality with regression comparison is Y = 24.418 – 0.014 X +1.187E – 05 X², with peak point is (543.76: 20.23) of correlation coefficient (r) 0.55 and determination coefficient (R²) as 30.34%. The addition of 600 μg testosterone level with 6 level dilutions was the best to defend sperm abnormality. (Animal Production 4(2): 60-70 (2002) Key Words : Spermatozoa, Kedu Chicken, Testosterone, Dilution, Speed Movement and Abnormality
The effect of Physiological NaCl Dilution levels and Storage Duration to Sperm Motility and Abnormality in Muscovy Duck Fauzi, MA; WS, Rachmawati; Pramono, Edy
ANIMAL PRODUCTION Vol 3, No 2 (2001): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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The aim of this research are to know the effects of physiological NaCl dilution levels and storage duration on sperm motility and abnormality of  Muscovy duck. Materials which used in this research were semen that collected of 1  - 1,5 years of four Muscovy duck. Semen were treated by physiological NaCl dilution levels nol , three, six and nine and storage duration ( t0 = 0 minute t1 =45minutes and t2=90 minute). Replication was three tapping periode . The method of this research was laboratory experimental with Randomized Complete Blok Design (RBD) in Split Plot Design (SPD)  pattern ,as main plot is Physiological NaCl dilation levels and as sub plot is storage duration. Result of the experiment showed that physiological NaCl dilution levels was very significantly influences on sperm motility (p<0,01) and significantly influences and sperm abnormality (p<0,05) . The storage duration was very significantly influences and sperm motility and abnormality (p<0,01) .Orthogonal duration polynomial assayed showed that the effect of physiological NaCl dilution levels to sperm motility  had a linier regression line with the a equation as Y =69.94 - 2.65 X,  r=0,49 R2=0.24 with Y presented to sperm motility and X represented to physiological NaCl dilution levels  sperm and effect of storage duration to sperm motility had a linier regression line with the equation as Y =74.24-0.35 X, r =0.73 R2=0.53 with Y represented to sperm motility and X represented to storage duration . The effect of physiological NaCl dilution levels to sperm abnormality had a linier regression line with the equation as Y =5.61+0.34X, r=0.52 R2=0.27 with Y represented to sperm to abnormality and X represented to regression line with the equation as Y =6.74+0.01X, r=0.24R2=0.060 with Y represented to sperm abnormality and X represented to storage duration. It is can be concluded that a higher physiological NaCl dilation levels and storage duration caused sperm to motility decreased and sperm abnormality increased. (Animal Production 3(2): 45-52 (2001)Key Word: sperm Muscovy duck.
Co-Authors Agus Susanto MA Fauzi, MA Mudjijono Mudjijono, Mudjijono Patiha Patiha, Patiha Rachmawati Wahyuningsih, Rachmawati Rachmawati WS, Rachmawati Yuniawan Hidayat