Agus Eko Prasetyo
Pusat Penelitian Kelapa Sawit, Medan

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EFFECT OF BAGWORM Pteroma pendula Joannis ATTACK ON THE DECREASE IN OIL PALM PRODUCTIVITY Priwiratama, Hari; Perdana Rozziansha, Tjut Ahmad; Susanto, Agus; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 19 No. 2 (2019): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.219101-108

Abstract

Outbreak of oil palm bagwormPteroma pendula has been reported within the last few years in several plantations in North Sumatra. A study was conductedto determine the impact of P. pendula attacks on oil palm productivity. Aerial photograph was taken in November 2016 andanalyzed to determine the level of damage on each palm canopy. The damage symptoms were classified onto healthy palms ascontrol plants (score 0), mild (score 1), moderate (score 2), severe (score 3), and very severe (score 4). The sample palms wererandomly selected and observed for the sex ratio, bunch number and bunch weight at 18 months after defoliation. The attackof P. pendula at the highest level (score 4) caused a significant effect on sex ratio and the number of bunches produced. Thefresh fruit bunches production was declined 21.02–36.35% on palms with moderate to very severe attack (score 2–4). Similarpalms also have a potential case of inflorescences abortion 18.41–32.54%. However, the average bunch weight was notinfluenced by P. pendula attack.
KARAKTERISASI FITOPLASMA PENYEBAB PENYAKIT LAYU KELAPA DI PULAU DERAWAN MENGGUNAKAN RFLP IN SILICO Mutaqin, Kikin Hamzah; ., Giyanto; Prasetyo, Agus Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.705 KB) | DOI: 10.23960/j.hptt.217105-110

Abstract

Characterization of phytoplasmas associated with coconut wilt disease in Derawan Island using in silico RFLP. Coconutwilt disease has been reported in Derawan Island that resulted in eradication up to 10% of the total cultivated palms. Theobjective of this study was to detect and characterize phytoplasmas associated with coconut wilt disease in Derawan islandusing nested PCR technique and in silico RFLP based on 16S rRNA gene sequences. Detection of phytoplasmas was performedusing nested PCR method, cloning of nPCR products, sequencing, and analysis of sequencing results using in silico RFLP.The results revealed that phytoplasmas could not be detected by PCR using P1/P7 primer pairs however it could be amplifiedby nested PCR using R16F2n/R16R2 primer pairs resulting amplicon at about 1.25 kb. In silico RFLP analysis indicated thatphytoplasmas associated with coconut wilt disease in Derawan Island belong to 16SrII (witches broom phytoplasma). PCRproduct of the nPCR need to be sequenced because the R16F2n/R16R2 primer will also amplify the other bacterial species, mainly from Gram positive bacteria.