Diding Heri Prasetyo
Laboratorium Biokimia Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Journal : Majalah Kedokteran Bandung

Efek Probiotik terhadap Mortalitas, Derajat Inflamasi Intestinal, dan Kadar IgA pada Mencit Model Sepsis Indrayanto, Yoseph; Prasetyo, Diding Heri
Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Sepsis mengubah mikrobiologi saluran pencernaan, menyebabkan hilangnya flora komensal dan pertumbuhan bakteri patogen yang berlebih. Pemberian probiotik penderita sepsis dapat mengembalikan keseimbangan mikrobiota dan memiliki efek positif pada fungsi imunitas tubuh serta struktur dan fungsi gastrointestinal. Penelitian ini bertujuan untuk menentukan efek pemberian probiotik terhadap mortalitas, inflamasi intestinal, dan kadar imunoglobulin A (IgA) serum pada mencit model sepsis. Penelitian ini merupakan penelitian eksperimental laboratoris, dengan sampel 36 ekor mencit Balb/C jantan dibagi menjadi kelompok: kontrol, sepsis, dan sepsis+probiotik. Penelitian dilakukan di laboratorium Histologi dan Biomedik Fakultas Kedokteran Universitas Sebelas Maret Surakarta,periode Januari–April 2012. Mencit Balb/C jantan diinjeksi cecal inoculum (200 mg/kgBB) secara intraperitoneal (i.p), untuk kelompok sepsis maupun sepsis dengan probiotik. Kelompok mencit kontrol tidak diinokulasi selama penelitian. Inflamasi intestinal ditentukan dengan pengecatan hematoksilin eosin (HE) pada hari ke-8. Mortalitas dinilai sampai hari ke-8. Kadar IgA serum ditentukan menggunakan enzyme-linked immunosorbent assay. Twotailed Fisher Exact Test untuk uji mortalitas, Kruskal-Wallis untuk uji inflamasi intestinal, sedangkan analysis of variance untuk uji kadar IgA serum. Persentase kematian kelompok probiotik tidak berbeda bermakna (10% vs 40%, p=0,065) dibandingkan dengan kelompok sepsis. Probiotik bermakna menurunkan derajat inflamasi intestinal dibandingkan dengan kelompok sepsis (p<0,001). Kadar IgA serum kelompok mencit kontrol 35,82±4,55 ng/mL. Probiotik secara bermakna meningkatkan kadar IgA serum (65,07±34,97 ng/mL vs 6,20±5,80 ng/mL, p<0,001) dibandingkan dengan kelompok mencit sepsis. Simpulan, pemberian probiotik menurunkan derajat inflamasiintestinal dan mortalitas, serta meningkatkan kadar IgA serum pada mencit model sepsis. [MKB. 2013;45(1):10–5]Kata kunci: IgA, inflamasi, probiotik, sepsis Probiotic Effects of Mortality, Degree of Intestinal Inflammation, and IgA Levels in Sepsis Mice ModelSepsis results in changes to the microbiology of the gastrointestinal tract, leading to a loss of commensal flora and an overgrowth of potentially pathogenic bacteria. Administering probiotics to sepsis patients may restore balance to the microbiota and have positive effects on immune function and gastrointestinal structure and function. This study was aimed to evaluate the effect of probiotic on mortality, intestinal inflammation and immunoglobulin A (IgA) serum level in mice model of sepsis. This study was an experimental research laboratory, with 36 male Balb/C mice were divided into: control, sepsis, and sepsis+probiotic groups. The study was conducted at Histology and Biomedical Laboratory, School of Medicine, Sebelas Maret University, Surakarta, in January to April 2012. Sepsis was induced in the male Balb/C mice using an intraperitoneally (i.p) injection of cecal inoculum (200 mg/ kgBW), for sepsis mice model and sepsis with probiotic. Control mice were not inoculation during the study. Detection of intestinal inflammation with hematoxylin and eosin (HE) staining on 8th day. Mortality perceived until 8th day. IgA serum level measurement with enzyme-linked immunosorbent assay. Two-tailed Fisher exact test for the analysis of mortality, Kruskal-Wallis test for the intestinal inflammation, one way analysis of variance for IgA serum level. Probiotic showed no significantly mortality (10% vs 40%, p=0.065) compared to sepsis group. Probiotic showed significantly decreased intestinal inflammation compared to sepsis group (p<0.001). Control mice showed levels of IgA 35.82±4.55 ng/mL. Probiotic showed significantly increased IgA levels (65.07±34.97 ng/mL vs 6.20±5.80 ng/mL, p<0.001) compared to sepsis group. In conclusions, probiotic decrease intestinal inflammation and mortality, on the other hand increase IgA level in mice model of sepsis. [MKB. 2013;45(1):10–5]Key words: IgA, inflammation, probiotic, sepsis DOI: http://dx.doi.org/10.15395/mkb.v45n1.95
Efek Probiotik pada Kadar IgA Mencit Model Sepsis Prasetyo, Diding Heri; Purwanto, Bambang
Majalah Kedokteran Bandung Vol 42, No 4
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Probiotik berfungsi sebagai imunomodulator dan imunonutrisi pada penderita penyakit kritis seperti sepsis, tetapi mekanismenya belum diketahui secara pasti. Penelitian ini bertujuan untuk menentukan efek pemberian probiotik pada kadar IgA mencit model sepsis di Rumah Sakit dr. Moewardi/Fakultas Kedokteran Universitas Sebelas Maret Surakarta periode Juli 2009–2010. Hewan uji berupa 18 ekor mencit Balb/C jantan yang dibagi menjadi tiga kelompok. Kelompok I tanpa diberi perlakuan (kontrol negatif), kelompok II adalah mencit model sepsis yang diinokulasi dengan lipopolisakarida (LPS)-E.coli (0,4 mg/mencit/i.p.), dan kelompok III adalah mencit model sepsis yang diinokulasi dengan LPS-E.coli serta diberi probiotik. Kadar IgA serum diperiksa dengan menggunakan ELISA. Data dianalisis secara statistik dengan one way ANOVA. Kadar IgA serum pada kelompok mencit normal 35,82±4,55 ng/mL. Pemaparan LPS-E.coli menurunkan kadar IgA serum menjadi 6,20±5,80 ng/mL. Pemberian probiotik pada mencit model sepsis, mampu meningkatkan kadar IgA serum menjadi 65,07±34,97 ng/mL. Probiotik secara bermakna meningkatkan kadar IgA serum dibandingkan dengan kelompok mencit sepsis (65,07±34,97 ng/mL vs 6,20±5,80 ng/mL, p=0,000). Simpulan, pemberian probiotik meningkatkan kadar IgA serum pada mencit model sepsis. [MKB. 2010;42(4):175–80].Kata kunci: IgA, probiotik, sepsisThe Effect of Probiotic on IgA Level in Mice Model of SepsisProbiotic is useful as immunomodulator and imunonutrition in patients with critical illness such as sepsis, but the mechanism is not entirely clear. This study was aimed to evaluate the effect of probiotic on serum IgA level in mice model of sepsis in dr. Moewardi Hospital/Faculty of Medicine, Universitas Sebelas Maret Surakarta period July 2009–2010. Eighteen males Balb/C mice were used and divided into three groups. Group I without treatment (negative control), group II which was mice model of sepsis inoculated with lipopolysaccharide (LPS)-E.coli (0.4 mg/mouse/ip), and group III was mice model of sepsis inoculated with E. coli LPS-and given probiotic. Serum IgA level was examined by ELISA. Data were analyzed statistically with one-way ANOVA. Serum IgA levels in normal mice group was 35.82±4.55 ng/mL. Exposing LPS-E. coli reduced the levels of serum IgA to 6.20±5.80 ng/mL. Administration of probiotics increased the serum IgA level to 65.07±34.97 ng/mL in mouse models of sepsis. Probiotics significantly increased serum IgA levels compared to mice model of sepsis group (65.07 ± 34.97 ng / mL vs. 6.20 ± 5.80 ng / mL, p = 0.000). In conclusion, administration of probiotics can increase the levels of serum IgA in mice model of sepsis. [MKB. 2010;42(4):175–80]. DOI: http://dx.doi.org/10.15395/mkb.v42n4.33
Ekstrak Etanol Propolis Isolat Menurunkan Derajat Inflamasi dan Kadar Malondialdehid pada Serum Tikus Model Sepsis Prasetyo, Diding Heri; Suparyanti, Endang Listyaningsih; Guntur H., A.
Majalah Kedokteran Bandung Vol 45, No 3 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Peran propolis lebah sebagai terapi adjuvan pada pengelolaan sepsis telah dievaluasi. Penelitian ini bertujuan untuk mempelajari efek perlindungan propolis pada sepsis akibat inokulasi cecal, derajat inflamasi intestinal, dan kadar malondialdehid (MDA) serum. Penelitian dilakukan di Laboratorium Histologi dan Biomedik, Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, selama periode April–September 2011. Empat puluh tikus Rattus norvegicus L jantan dibagi menjadi lima kelompok: kontrol, sepsis, sepsis+antibiotik, sepsis+propolis, dan sepsis+antibiotik+propolis. Pada hari kedelapan, semua hewan coba dikorbankan untuk diukur konsentrasi MDA serum dan perubahan histopatologi di intestinal. Uji konsentrasi MDA serum menggunakan analysis of variance (ANOVA), sedangkan derajat inflamasi intestinal dengan Uji Kruskall-Wallis, dan untuk menentukan perbedaan kemaknaan digunakan p<0,05. Kadar MDA serum masing-masing kelompok: kontrol 0,27±0,07 μmol/L; sepsis 0,39±0,05 μmol/L; sepsis+antibiotik 0,15±0,03 μmol/L; sepsis+ propolis 0,09±0,05 μmol/L; dan sepsis+antibiotik+propolis 0,21±0,11 μmol/L. Derajat inflamasi intestinal menunjukkan kelompok kontrol derajat 0–3; sepsis derajat 3–4; sepsis+antibiotik derajat 1–3; sepsis+propolis 1–3; dan sepsis+antibiotik+propolis derajat 0–2. Simpulan, ekstrak etanol propolis menurunkan derajat inflamasi intestinal dan kadar MDA serum pada hewan coba model sepsis. [MKB. 2013;45(3):161–6] Ethanol extract of Propolis Reduces the Level of Inflammation and Serum Malondialdehyde in Sepsis Rats ModelThe role of bee propolis as a adjuvant therapy in the management of sepsis was evaluated. The aim of the study was to investigate the protective effect of propolis against cecal inoculum induced sepsis, the level of intestinal inflammation, and serum malondialdehyde (MDA) concentrations. The study was conducted at School of Medicine, Sebelas Maret University, Surakarta, in April to September 2011. Forty male Rattus norvegicus L rats were divided into five groups: control, sepsis, sepsis+antibiotic, sepsis+propolis and propolis+antibiotic+sepsis groups. On the eighth day, all experimental animals were sacrificed. Serum concentrations of MDA were evaluated. In addition, the histopathological changes in intestinal were assessed. Kruskall-Wallis test with Mann-Whitney analysis were used to determine significant differences. Results were expressed as mean±standard error of the mean, and value of p<0.05 was considered statistically significant. Malondialdehyde serum means levels were control group 0.27±0.07 μmol/L, sepsis 0.39±0.05 μmol/L, sepsis+antibiotic 0.15±0.03 μmol/L, sepsis+ propolis 0.09±0.05 μmol/L, and sepsis+antibiotic+propolis 0.21±0.11 μmol/L respectively. The levels of intestinal inflammation were control groups 0 to 3, sepsis 3 to 4, sepsis+antibiotic 1 to 3, sepsis+propolis 1 to 3, and sepsis+antibiotic+propolis 0 to 2, respectively. In conclusions, ethanol extract of propolis reduces the levels of intestinal inflammation and serum MDA in sepsis animal models. [MKB. 2013;45(3):161–6] DOI: http://dx.doi.org/10.15395/mkb.v45n3.146
Efek Kortikosteroid Dosis Rendah terhadap Kadar Soluble Receptor for Advanced Glycation End Products Mencit Balb/C Model Sepsis Prasetyo, Diding Heri; Subandono, Jarot; Aisyah, Siti
Majalah Kedokteran Bandung Vol 47, No 1 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Penggunaan kortikosteroid dosis rendah pada penatalaksanaan sepsis tahap awal masih diperdebatkan. Soluble receptor for advanced glycation end products (sRAGE) merupakan biomarker keparahan dan keluaran yang buruk pada sepsis. Penelitian ini bertujuan untuk menganalisis efek penggunaan kortikosteroid dosis rendah terhadap kadar sRAGE serum pada mencit Balb/C model sepsis tahap awal. Penelitian ini merupakan penelitian eksperimental laboratoris dengan sampel 30 ekor mencit Balb/C jantan dibagi menjadi kelompok kontrol, sepsis, dan sepsis+kortikosteroid dosis rendah. Penelitian dilakukan di laboratorium Histologi dan Biomedik Fakultas Kedokteran Universitas Sebelas Maret Surakarta periode Juni-Desember 2013. Untuk membuat model sepsis, hewan coba diinokulasi lipopolisakarisa/LPS (E. coli) dosis 0,1 mg/mencit secara intraperitoneal (i.p.). Kelompok mencit kontrol tidak diinokulasi selama penelitian. Kortikosteroid dosis rendah yang digunakan adalah metil prednisolon dosis 0,05 mg/mencit/hari secara i.p. Mencit kontrol tidak diinokulasi selama penelitian. Kadar sRAGE kelompok kontrol 54,29±16,28 pg/mL, sepsis 78,12±13,38 pg/mL, dan kortikosteroid dosis rendah 63,39±11,07 pg/mL. Kortikosteroid dosis rendah secara bermakna menurunkan kadar sRAGE (p=0,044) dibanding dengan kelompok sepsis. Simpulan, penggunaan kortikosteroid dosis rendah menurunkan kadar sRAGE pada hewan coba model sepsis tahap awal. [MKB. 2015;47(1):10–5]Kata kunci: Kortikosteroid dosis rendah, sepsis, sRAGEEffects of Low-Dose Corticosteroid on Soluble Receptor for Advanced Glycation End Products  in Balb/C Mice Sepsis Model The use of low-dose corticosteroids in the management of early sepsis is still under debate. Soluble receptor for advanced glycation end products (sRAGE) is a biomarker of severity and poor outcome of sepsis. This study aimed to analyze the effects of the use of low-dose corticosteroids on sRAGE serum levels in Balb/C mice model of early sepsis. This study was an experimental research laboratory study with 30 male Balb/C mice which divided into control, sepsis and sepsis+low-dose corticosteroids groups. The study was conducted at Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, from June to December 2013. Sepsis was induced in the male Balb/C mice by inoculation with an intraperitoneally (i.p.) injection of lipopoly-saccharide/LPS (E. coli) with a dose of 0.1 mg/mice/i.p.for sepsis mice model. Control mice were not inoculated during the study. Low-dose corticosteroids used was methyl prednisolone at a dose of 0.05 mg/mice/day/i.p. Levels of sRAGE  54.29±16.28 pg/mL in control group, 78.12±13.38 pg/mL in sepsis group, and 63.39±11.07 pg/mL in low-dose corticosteroids group. Low-dose corticosteroids significantly decreased sRAGE level (p=0.044) compared to the sepsis group. In conclusion, the use of low-dose corticosteroids reduces levels of sRAGE in early sepsis.   [MKB. 2015;47(1):10–5]Key words: Low-dose corticosteroids, sepsis, sRAGE DOI: 10.15395/mkb.v47n1.392   
PENURUNAN KADAR PRO-ADRENOMEDULLIN MENCIT BALB/C MODEL SEPSIS DENGAN KORTIKOSTEROID DOSIS RENDAH Hermawan, A. Guntur; Nurwati, Ida; Prasetyo, Diding Heri; Indrayanto, Yoseph
Majalah Kedokteran Bandung Vol 46, No 2 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Penggunaan kortikosteroid dosis rendah pada penatalaksanaan sepsis tahap awal masih diperdebatkan. Pemberian kortikosteroid dosis rendah pada sepsis dapat mengurangi respons inflamasi sitemik, menghambat produksi sitokin proinflamasi dan mediator inflamasi, serta menurunkan adhesi leukosit ke endotel. Pro-adrenomedullin (pro-ADM) merupakan biomarker keparahan sepsis. Penelitian ini bertujuan untuk menganalisis efek penggunaan kortikosteroid dosis rendah pada kadar pro-ADM serum pada mencit Balb/C model sepsis tahap awal. Penelitian ini merupakan penelitian eksperimental laboratoris, dengan sampel 30 ekor mencit Balb/C jantan yang dibagi menjadi kelompok kontrol, sepsis, dan sepsis+kortikosteroid dosis rendah. Penelitian dilakukan di Laboratorium Histologi dan Biomedik, Fakultas Kedokteran, Universitas Sebelas Maret Surakarta, selama periode April?September 2012. Untuk membuat model sepsis, hewan coba diinokulasi lipopolisakarisa/LPS (E. coli) dosis 0,1 mg/mencit secara intraperitoneal (i.p). Kelompok tikus kontrol tidak diinokulasi selama penelitian. Kortikosteroid dosis rendah yang digunakan adalah metil prednisolon dosis 0,05 mg/mencit/12 jam secara i.p. Mencit kontrol tidak diinokulasi selama penelitian. Kadar pro-ADM serum ditentukan menggunakan enzyme-linked immunosorbent assay (ELISA). Uji kadar pro-ADM serum menggunakan analysis of variance (ANOVA) dan untuk menentukan perbedaan kemaknaan digunakan p<0,05. Kortikosteroid dosis rendah secara bermakna menurunkan kadar pro-ADM dibandingkan dengan kelompok sepsis (33,0±2,9 pg/mL vs 48,5±6,1 pg/mL; p<0,001). Simpulan, penggunaan kortikosteroid dosis rendah menurunkan kadar pro-ADM pada hewan coba model sepsis tahap awal. [MKB. 2014;46(2):68?72]Kata kunci: Kortikosteroid dosis rendah, pro-adrenomedullin, sepsisPro-adrenomedullin Level Reduction in Balb/C Sepsis Model Mice through the Use of Low-Dose CorticosteroidThe use of low-dose corticosteroids in the management of early stage of sepsis is still debated. Low-dose corticosteroids in sepsis can reduce the systemic inflammatory response, inhibit the production of pro-inflammatory cytokine, inflammatory mediators, and decrease adhesion of leukocytes to the endothelium. Pro-adrenomedullin (pro-ADM) is a biomarker of sepsis severity. This study aimed to analyze the effects of the use of low-dose corticosteroids on the levels of pro-ADM in the serum in Balb/C mice model of sepsis at the early stage. This study was an experimental research laboratory, with 30 male Balb/C mice which were divided into control, sepsis, and sepsis+low-dose corticosteroids groups. The study was conducted at the Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, during the period of April to September 2012. Sepsis was induced in the male Balb/C mice by inoculation through an intraperitoneally (i.p) injection of lipopolysaccharide/LPS (E. coli) with 0.1 mg/mice/i.p. dose for sepsis mice model. Control mice were not inoculated during the study. Low-dose corticosteroids used was methyl prednisolone at a dose of 0.05 mg/mice/12 hours/i.p. Pro-ADM serum level was measured with enzyme-linked immunosorbent assay (ELISA). One way analysis of variance (ANOVA) for pro-ADM serum level and p<0.05 were used to determine the significant differences. Low-dose corticosteroids significantly decreased pro-ADM level (33.0±2.9 pg/mL vs 48.5±6.1 pg/mL, p<0.001) compared to the sepsis group. In conclusion, the use of low-dose corticosteroids reduce levels of pro-ADM in the early stages of sepsis. [MKB. 2014;46(2):68?72]Key words: Low-dose corticosteroids, pro-adrenomedullin, sepsis DOI: 10.15395/mkb.v46n2.276