Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Bioma : Berkala Ilmiah Biologi

Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L). Wandita, Ryan Hilda; Pujiyanto, Sri; Suprihadi, Agung; Hastuti, Ratih Dewi
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.607 KB) | DOI: 10.14710/bioma.20.1.9-16

Abstract

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN
Produksi Miselium Jamur Ling ZHI (Ganoderma lucidum) Dalam Medium Air Kelapa Tua Dan Tauge Extract Broth Dengan Metode Kultur Terendam Teragitasi Sulistyaningtyas, Ayu Rahmawati; Suprihadi, Agung
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.739 KB) | DOI: 10.14710/bioma.19.1.58-61

Abstract

Jamur ling zhi (Ganoderma lucidum) sudah mulai dibudidayakan secara intensif di Indonesia. Masalah yang sering dihadapi dalam budidaya jamur ling zhi adalah kesulitan dalam penyediaan bibit. Proses penyediaan bibit jamur biasanya dilakukan secara konvensional menggunakan media agar. Metode konvensional akan membutuhkan biaya dan waktu produksi yang banyak sehingga diperlukan metode pembibitan baru yaitu kultur terendam teragitasi. Kultur terendam miselium dari kultur murni langsung dapat ditanam pada media tanam jamur. Penelitian ini bertujuan untuk mengetahui perbandingan produksi miselium jamur ling zhi dalam medium air kelapa tua dan tauge extract broth dengan metode terendam teragitasi. Parameter  utama penelitian meliputi berat kering miselium, sedangkan parameter pendukung meliputi suhu inkubasi, pH medium awal dan akhir inkubasi. Penelitian dilakukan dengan rancangan acak lengkap faktorial (RAL) dengan faktor medium. Hasil penelitian menunjukkan bahwa produksi miselium jamur ling zhi (G. Lucidum) dalam medium air kelapa tua lebih tinggi dibandingkan medium tauge extact broth. Kata kunci: Ganoderma lucidum, Medium, Air Kelapa Tua, Tauge Extract Broth, Kultur Terendam Teragitasi
Diversitas Kapang Serasah Daun Talok (Muntingia calabura L.) Di Kawasan Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madur Miranti, Arum Krisna; Rukmi, MG Isworo; Suprihadi, Agung
Bioma : Berkala Ilmiah Biologi Vol. 16, No.2, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.403 KB) | DOI: 10.14710/bioma.16.2.58-64

Abstract

Leaf litter is one kind of substrate which is good for mold’s growth because of its organic matters content particularly cellulose. Research on mold diversity on Talok (Muntingia calabura L.) leaf litter which grow abundance at Sukolilo Barat Village, Labang Subdistrict, Bangkalan, Madura was conducted in order to determined the diversity and to isolate xerophilic mold as well as to examined the enzymatic activity of the isolates. The isolation has been done by direct and indirect method on DG18 agar, MEA, and OA from three samples which is taken purposively. The enzyme activities observed were cellulolytic, amylolitic and proteolytic at 310C temperature. The results showed that 24 isolates found were come from 3 genus i.e. Aspergillus, Curvularia and Fusarium. The Aspergillus was the largest number found. The Shannon-Wiener Index of Diversity showed that the diversity of three leaf litter samples categorized medium. The highest cellulolytic, amylolitic and proteolytic activity at 310C incubation were showed by A. tamarii (ISM 1), A.aculeatus (ISM 10) , A. terreus sp. 3 (ISM 17) respectively. Keywords : Diversity, mold, talok litter leaf, Madura
Isolasi dan Karakterisasi Plant Growth Promoting Rhizobacteria dari Perakaran Kelapa Sawit pada Lahan Gambut Ariyani, Mei Dwi; Dewi, Tirta Kumala; Pujiyanto, Sri; Suprihadi, Agung
Bioma : Berkala Ilmiah Biologi Vol. 23, No 2, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.2.159-171

Abstract

Peatlands have characteristics of low pH and lack nutrients. Oil palm is the main plant commodity in peatland management. Oil palm roots have been known to be a nutrition source for the growth of soil microbes, especially bacteria around their roots or PGPR. PGPR are a group of bacteria that play an important role in supporting plant growth and health. The purpose of this research was to obtain PGPR potential from oil palm roots which can be used as candidates for biofertilizer agents. In this study, the isolation and selection of PGPR isolate from oil palm roots on oil palm plantations in Central Kalimantan were carried out based on their plant growth-promoting traits, including the activity of producing Indole Acetic Acid (IAA), phosphate solubilizing, N-fixing, K solubilizing, siderophore production, ACC deaminase activity, proteolytic activity, cellulolytic activity, and ligninolytic activity. A total of 17 isolates were selected to be tested for their multiple activities ability. The final results of the PGPR characterization showed that of the seventeen isolates, all isolates had PGPR activity at least three different abilities. From the seventeen isolates, it was found that the SW 5.5 PK 3A isolate had the highest IAA production activity (58,50 ppm), SW 4.10 PK 1A isolate had the highest K solubilizing index (3,16), SW 8.5 PK 1A isolate had both the highest P solubilizing index (3,73) and the highest siderophore zone index (5,20), SW 4.11 PK isolate had the highest proteolytic index (4,80), and SW 4.10 PK 1A.P isolates had the highest cellulolytic index (5,11).