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Produksi Miselium Jamur Ling ZHI (Ganoderma lucidum) Dalam Medium Air Kelapa Tua Dan Tauge Extract Broth Dengan Metode Kultur Terendam Teragitasi Sulistyaningtyas, Ayu Rahmawati; Suprihadi, Agung
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.739 KB) | DOI: 10.14710/bioma.19.1.58-61

Abstract

Jamur ling zhi (Ganoderma lucidum) sudah mulai dibudidayakan secara intensif di Indonesia. Masalah yang sering dihadapi dalam budidaya jamur ling zhi adalah kesulitan dalam penyediaan bibit. Proses penyediaan bibit jamur biasanya dilakukan secara konvensional menggunakan media agar. Metode konvensional akan membutuhkan biaya dan waktu produksi yang banyak sehingga diperlukan metode pembibitan baru yaitu kultur terendam teragitasi. Kultur terendam miselium dari kultur murni langsung dapat ditanam pada media tanam jamur. Penelitian ini bertujuan untuk mengetahui perbandingan produksi miselium jamur ling zhi dalam medium air kelapa tua dan tauge extract broth dengan metode terendam teragitasi. Parameter  utama penelitian meliputi berat kering miselium, sedangkan parameter pendukung meliputi suhu inkubasi, pH medium awal dan akhir inkubasi. Penelitian dilakukan dengan rancangan acak lengkap faktorial (RAL) dengan faktor medium. Hasil penelitian menunjukkan bahwa produksi miselium jamur ling zhi (G. Lucidum) dalam medium air kelapa tua lebih tinggi dibandingkan medium tauge extact broth. Kata kunci: Ganoderma lucidum, Medium, Air Kelapa Tua, Tauge Extract Broth, Kultur Terendam Teragitasi
Diversitas Kapang Serasah Daun Talok (Muntingia calabura L.) Di Kawasan Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madur Miranti, Arum Krisna; Rukmi, MG Isworo; Suprihadi, Agung
Bioma : Berkala Ilmiah Biologi Vol. 16, No.2, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.403 KB) | DOI: 10.14710/bioma.16.2.58-64

Abstract

Leaf litter is one kind of substrate which is good for mold’s growth because of its organic matters content particularly cellulose. Research on mold diversity on Talok (Muntingia calabura L.) leaf litter which grow abundance at Sukolilo Barat Village, Labang Subdistrict, Bangkalan, Madura was conducted in order to determined the diversity and to isolate xerophilic mold as well as to examined the enzymatic activity of the isolates. The isolation has been done by direct and indirect method on DG18 agar, MEA, and OA from three samples which is taken purposively. The enzyme activities observed were cellulolytic, amylolitic and proteolytic at 310C temperature. The results showed that 24 isolates found were come from 3 genus i.e. Aspergillus, Curvularia and Fusarium. The Aspergillus was the largest number found. The Shannon-Wiener Index of Diversity showed that the diversity of three leaf litter samples categorized medium. The highest cellulolytic, amylolitic and proteolytic activity at 310C incubation were showed by A. tamarii (ISM 1), A.aculeatus (ISM 10) , A. terreus sp. 3 (ISM 17) respectively. Keywords : Diversity, mold, talok litter leaf, Madura
Isolasi dan Karakterisasi Bakteri Endofit Pelarut Fosfat dan Penghasil Hidrogen Cyanide (HCN) dari Tanaman Bawang Merah (Allium cepa L). Wandita, Ryan Hilda; Pujiyanto, Sri; Suprihadi, Agung; Hastuti, Ratih Dewi
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.607 KB) | DOI: 10.14710/bioma.20.1.9-16

Abstract

Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN
MIX CULTURE INOCULANT PRODUCTION OF PHOSPHATE SOLUBILYZING AND INDOLE ACETIC ACID (IAA) PRODUCER RHIZOBACTERIA WITH AMBARAWA PEAT SOIL RAWAPENING AS CARRIER Raharjo, Budi; Suprihadi, Agung
JURNAL SAINS DAN MATEMATIKA Volume 18 Issue 2 Year 2010
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6092.991 KB)

Abstract

ABSTRACT---Plant production development  is the main goals  that do for increase the farming quality to fulfill the man needed  in  food. One of the ways is intensive farming, by using organic or inorganic  fertilizer. Phosphate is the essential  for plants. IAA is the necessary plant regulator for the root. Both phosphate  and IAA need in  plant  growing  and production.  Biological  fertilizer  is  fertilizer  with  microbial  as the main material. Bacillus sp.DUCC-BR-K1.7, Bacillus sp. DUCC-BR-KI3, Bacillus sp.DUCC-BR-KI.2b  and Pseudomonas fluorescens, Azotobacter chroococcum, Azotobqcter vinelandii, Azospirillum sp. and Azotobacter brazilensis are bacteria that can solubilization  the phosphate  and IAA  synthesize.  Those bacteria can be used as  inoculants or biological  fertilizer  that put on carrier. One way to support  the aim is giving  the altemative carrier with  suitable composition. The carrier should be support bacterial life  during the storage.  The aim ofthis  research  is find  ths right  consortia so can be used to optimized  viabilities  of  culture Bacillus  sp. DUCC-BR-KIJ,  Bacillus sp. DUCC-BR-K1.3, Bacillus sp. DUCC-BR-KI.2b, P. flourescens, A. chroococcum, A. vinelandii, Azospirillum  sp., and  A. brazilensis in mix  culture  on peat soil  as carrier. This research done in Microbiology Laboratory of Biology Department Diponegoro University. Subculture and activated culture in Nutrient  Broth medium, make the growth curve to the biomass production, make the inoculums, prepare the peat soil, biomass production and mixed biomass with  the carrier, enumeration bacterial culture viability  test in carrier during the storage  by TPC method.  The  results  show  that  all  consortia  culture  bacteria viabiliry Bacillus  sp. DUCC-BR-KI.3  with Azospirillum (A) and Bacillus  sp. DUCC-BR-K1.7, with P.  flourescens (B)  still viable and increasing number of population during seven weeks storage with  l0 t-n CFU/g at To and up to  l0 r3-ro CFU/g at  the end storage. The  consortium  C  between Bacillus  sp.  DUCC-BR-KI.2  b  and A-  brssilensis  increasing  the  number  of population  and  still viable during eight weeks  storage  (1013  - 1014  CFU/g).Key words: biofertilizer, peat soil, viability,  mix cultare, consortiumPermalink : http://ejournal.undip.ac.id/index.php/sm/article/view/3138
KARAKTERISASI BIODEGRADASI SENYAWA POLIAROMATIK DIBENZOTHIOPHENE OLEH BAKTERI LAUT NOVOSPHINGOBIUM MATHURENSE LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic
Identification of Gamma-Amino Butyric Acid Isolates Lactic Acid Bacteria Results from The Isolation of Rusip Prayitno, S Feta Avila; Suprihadi, Agung; Kusdiyantini, Endang
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24995

Abstract

Gamma-aminobutyric acid (GABA) is a non-protein amino acid produced by glutamate decarboxylation by the enzyme glutamate decarboxylase and it is widely distributed in plants, animals and microorganisms. GABA-producing microorganisms including Lactic Acid Bacteria (LAB) which play a role in the fermentation process of food such as rusip. Rusip is a preserved food of marine fish made from raw anchovy of Bangka-Belitung. The purpose of this study was to obtain LAB isolates through the process of isolation from rusip and identify the presence of GABA. Isolation of LAB was carried out by the pour method on MRS agar and NA medium. The growing colonies were then characterized based on observations of colony morphology, Gram staining, catalase test, motility test, and fermentation type test. LAB isolates obtained were selected to obtain isolates which are capable to produce GABA using the Thin Layer Chromatography (TLC) method. The results of isolation from rusip and inoculation on MRS agar medium obtained, three LAB isolates namely RSP-A1, RSP-A2, and RSP-A3. Their characteristics are Gram positive bacteria, negative catalase test, negative motility test, and belong to homofermentative and heterofermentative bacteria groups. They were also able to produce GABA. Research on GABA from isolates isolated from rusip is a new thing in the world of GABA research. This research provides information that is beneficial to produce GABA easily, cheaply, and efficiently.
Karakterisasi bakteri diazotrof dan pengaruhnya terhadap pertumbuhan tanaman kedelai (Glycine max L. Merril) Astuti, Luluk Aviyani Dwi; Muslichah, Dwi Agustiyani; Suprihadi, Agung; Rukmi, MG Isworo
NICHE Journal of Tropical Biology Vol. 4, No. 1, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.1.40-49

Abstract

Web-based Patient Queuing System and Automatic Notification via WhatsApp Framework Codeigniter Arnoldus, Arnoldus; Suprihadi, Suprihadi
International Journal of Natural Science and Engineering Vol 5, No 2 (2021): July
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.244 KB) | DOI: 10.23887/ijnse.v5i2.36766

Abstract

At present, the benefits of website-based information systems have been widely felt, but there are still many agencies that have not properly utilized the existence of technology. One of the systems that need to be developed in the health sector is giving queue numbers to patients. This study aimed to determine the effectiveness of the web-based patient queuing system and automatic notifications through the WhatsApp framework Codeigniter. The subjects involved in this study were patients who would take a queue number at the clinic. Data collection in the study was carried out by observation and interview methods. The research data were then analyzed using descriptive analysis techniques, namely by describing various findings in the field, which were then linked to the results of previous studies. The system development results show that the system’s success rate for giving queue numbers to patients reaches 100%, and the natrian number reminder system also gets a success percentage of 100%. These results indicate that the queue number system has been successfully developed to help patients search for queue numbers.
Isolasi dan Karakterisasi Plant Growth Promoting Rhizobacteria dari Perakaran Kelapa Sawit pada Lahan Gambut Ariyani, Mei Dwi; Dewi, Tirta Kumala; Pujiyanto, Sri; Suprihadi, Agung
Bioma : Berkala Ilmiah Biologi Vol. 23, No 2, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.2.159-171

Abstract

Peatlands have characteristics of low pH and lack nutrients. Oil palm is the main plant commodity in peatland management. Oil palm roots have been known to be a nutrition source for the growth of soil microbes, especially bacteria around their roots or PGPR. PGPR are a group of bacteria that play an important role in supporting plant growth and health. The purpose of this research was to obtain PGPR potential from oil palm roots which can be used as candidates for biofertilizer agents. In this study, the isolation and selection of PGPR isolate from oil palm roots on oil palm plantations in Central Kalimantan were carried out based on their plant growth-promoting traits, including the activity of producing Indole Acetic Acid (IAA), phosphate solubilizing, N-fixing, K solubilizing, siderophore production, ACC deaminase activity, proteolytic activity, cellulolytic activity, and ligninolytic activity. A total of 17 isolates were selected to be tested for their multiple activities ability. The final results of the PGPR characterization showed that of the seventeen isolates, all isolates had PGPR activity at least three different abilities. From the seventeen isolates, it was found that the SW 5.5 PK 3A isolate had the highest IAA production activity (58,50 ppm), SW 4.10 PK 1A isolate had the highest K solubilizing index (3,16), SW 8.5 PK 1A isolate had both the highest P solubilizing index (3,73) and the highest siderophore zone index (5,20), SW 4.11 PK isolate had the highest proteolytic index (4,80), and SW 4.10 PK 1A.P isolates had the highest cellulolytic index (5,11).
ANALISIS PERBANDINGAN KEANDALAN SISTEM JARINGAN DISTRIBUSI BERKONFIGURASI RADIAL DAN LOOP MENGGUNAKAN METODE RIA (RELIABILITY INDEX ASSESSMENT) H.R.B Moch Gozali; Suprihadi Prasetyono; Dimas Oktasya Eka Kumala Putra
Jurnal Arus Elektro Indonesia Vol 6 No 3 (2020)
Publisher : Fakultas Teknik, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jaei.v6i3.19723

Abstract

Electrical energy is a very important requirement for human life. The supply of electrical energy itself consists of electricity generation, power transmission and distribution. In the distribution of electricity, there are several networks that function to distribute electrical energy to customers, the majority of which are network configuration, namely radial and loop. In order to maintain the continuity of the distribution of electrical energy, a good level of reliability is required. In order to determine the reliability of the distribution system, the reliability index is determined, namely SAIFI, SAIDI and CAIDI. Several methods that can be used to find a distribution system reliability index include the RIA (Reliability Index Assessment) method, which is an approach used to predict disturbances in a distribution system based on the system topology and data regarding component reliability. In this study, we compared two configurations using one feeder, namely the Giri feeder which is assumed to have a radial configuration and a loop configuration. By using the RIA (Reliability Index Assessment) method on a Giri feeder with a radial configuration, the SAIFI reliability index is obtained at 1.595 times / year, SAIDI is 12.8092 hours / year, CAIDI is 8.0309 hours / year, while for a feeder with a loop configuration the SAIFI value is equal to 1,651 times / year, SAIDI is 1,7276 hours / year, CAIDI is 1,0416 hours / year. Keywords ­— Reliability Index, Distribution System, Reliability Index Assessment, SAIFI, SAIDI, CAIDI