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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia The Journal of Experimental Life Sciences (JELS) Media Penelitian dan Pengembangan Kesehatan Biogenesis: Jurnal Ilmiah Biologi Journal of Innovation and Applied Technology Research Journal of Life Science Natural B Jurnal Ilmiah Perikanan dan Kelautan REKAYASA International Journal of Applied Biology JURNAL ENGGANO Jurnal Sumberdaya Akuatik Indopasifik Jurnal Penelitian Pendidikan IPA (JPPIPA) JPM (Jurnal Pemberdayaan Masyarakat) Indonesia Journal of Halal Samakia: Jurnal Ilmu Perikanan Jurnal Kelautan Nasional Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Aquaculture and Fish Health Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Perikanan Pantura Berkala Penelitian Hayati

Asep Awaludin Prihanto

Brawijaya University

Author-ID : 347226

Religion Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Metode Sederhana dan Efektif Untuk Penghitungan dan Visualisasi Tiga Dimensi (3D) Biofilm Vibriio Cholera Prihanto, Asep Awaludin; Sukoso, Sukoso; Fadjar, Mohamad; Kurniawan, Andi
Media Penelitian dan Pengembangan Kesehatan Vol 25, No 3 Sep (2015)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3588.565 KB)

AbstrakMikroorganisme yang mampu menghasilkan biofim menimbulkan masalah yang serius dalam bidang kesehatan dan pangan. Penelitian biofim bagi sebagian peneliti sangat identik dengan kerumitan proses penghitungan dan visualisasi penutupan permukaan substrat penempelan bakteri. Penelitian ini ditujukan untuk mengetahui efiiensi metode alternatif untuk menghitung dan memvisualisasikan biofimVibrio cholera. Pada penelitian ini beberapa faktor lingkungan seperti pH, suhu, dan kondisi kultur diujicobakan untuk mengetahui pengaruhnya terhadap pembentukan biofim V. cholera. Pembentukan biofim dihitung berdasarkan Biofim Coverage Rate (BCR) yang selanjutnya divisualisasikan menjadi bentuk tiga dimensi (3D) dengan memanfaatkan software Image-J. Hasil penelitian menunjukkan bahwa pH, suhu, dan kondisi kultur mampu memberikan pengaruh yang signifian terhadap pembentukan biofim Vibrio cholera. Metode alternatif yang digunakan dalam penelitian ini mampu menghitung BCR serta menggambarkannya dalam bentuk 3D dengan efiien sehingga dapat dijadikan alternatif analisis biofim bakteri.Â AbstractMicroorganism which produces biofim, will causes serious issues in health and safety of food. Researches in biofim are identic with the complexities and relatively laborious tasks especially on assaying and visualizing method of biofim. This study was aimed to calculate and visualize biofims produced by Vibrio cholera. In this study several environmental factors such as pH, temperature, andculture conditions have been tested to determine its inflence on biofim formation of Vibrio cholerae.Biofim formations were calculated and visualized based on Biofim coverage rate (BCR) and threedimensions (3D) structure using Image-J software. The results showed that pH, temperature, and cultureconditions had a signifiant inflence on the formation of V. cholerae biofim. The alternative method thatwas used in this study could effiiently calculate and visualize BCR to 3D structures. Therefore, here wereport an alternative method for calculating bacterial biofim.

PENGGUNAAN SOFTWARE IMAGE-J UNTUK PENGHITUNGAN DAN VISUALISASI 3D TUTUPAN BIOFILM VIBRIO CHOLERAE EL TOR PADA KONDISI TUMBUH BERBEDA Prihanto, Asep Awaludin
Rekayasa Vol 4, No 2: Oktober 2011
Publisher : Universitas Trunojoyo Madura

Biofilm adalah sekumpulan mikroorganisme yang menempel dalam suatu permukaan dengan perantara matrik eksoplisakarida. Mikroorganisme dalam bentuk biofilm ternyata menjadi sumber kontaminasi sekunder di dalam produk pangan. Kenyataan ini menjadi latar belakang utama bagi peneliti mikrobiologi pangan untuk mempelajari biofilm. Penelitian biofilm bagi sebagian peneliti sangat identik dengan kerumitan proses penghitungan dan visualisasi penutupan permukaan substrat penempelan bakteri. Penelitian ini ditujukan untuk menghitung dan memvisualisasikan biofilm dengan cara sederhana dan mudah melalui software image-J. Pada penelitian ini beberapa faktor lingkungan seperti pH, suhu, dan kondisi kultur telah diujicobakan untuk mengetahui pengaruhnya terhadap pembentukan biofilm Vibrio Cholerae El Tor. Pembentukan biofilm dihitung berdasarkan angka tutupan (Biofilm Coverage Rate) yang kemudian divisualisasikan. Hasil penelitian menunjukkan bahwa pH, suhu, salinitas dan kondisi kultur mampu memberikan pengaruh yang signifikan terhadap pembentukan biofilm Vibrio Cholerae El Tor. Image-J mampu menghitung dan menggambarkan angka tutupan dengan baik dan dapat dijadikan alternatif software analisis biofilm bakteri. Kata kunci: image-J, biofilm, Vibrio Cholera El Tor AbstractBiofilms are microorganisms that attached to a surface with eksoplisakarida matrix. Microorganisms in a biofilm was known to be a secondary source of contamination in food products. This fact trigger many scientist and food microbiologist conducting research biofilms. Research in biofilm for many researchers are identical with the complexity of the assaying and visualizing surface-covered area of biofilm.. This study was aimed to calculate and visualize biofilms in a simple and easy way through software image-J. In this study several environmental factors such as pH, temperature, and culture conditions have been tested to determine its influence on biofilm formation of Vibrio Cholerae El Tor. Biofilm formation was calculated based on Biofilm Coverage Rate (BCR) and then was visualized. The results showed that pH, temperature, salinity and culture conditions had a significant influence on the formation of Vibrio Cholerae El Tor biofilm. This result illustrated that Image-J can be able to calculate and to precisely describe the BCR visualization, therefore it can be used as alternatives software for bacterial biofilms analysis. Key words: image-J, biofilm, Vibrio Cholera El Tor

PERBANDINGAN AKTIVITAS ANTIBAKTERI Penicillium notatum ATCC 28089 DENGAN Penicillium sp. R1M YANG DIISOLASI DARI MANGROVE Sonneratia caseolaris Asep Awaludin Prihanto
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15 No 1 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Mangrove Sonneratia caseolaris is a potential host for endhopytic fungi that commonly produce strong dan unique antibiotic. The objective of the research was to compare the antibacterial activity of Penicillium notatum ATCC 28089 and endophytic fungus Penicillium sp. R1M isolated from Mangrove Sonneratia caseolaris. First step of the research was observation the growth curve of each isolate. Second step was isolation of their metabolites to be used in the antibacterial activity assay against Staphylococcus aureus ATCC 9144 and Escherichia coli ATCC 8739. The results showed that both isolates indicated different growth curves. Penicillium sp. R1M experienced shorter adaptation phase, than P. notatum ATCC 28089. The secondary metabolites of P. notatum ATCC 28089 was isolated on day 8, while those of Penicillium sp. R1M isolated on day 6. The result of antibacterial assay confirmed that the extracts of mycelia Penicillium sp. R1M show inhibition diameter in the growth of S. aureus and E. coli with 11.5 ± 0.6 mm and 10.6 ± 0.4, mm, respectively, while the medium extracts of P. notatum ATCC 28089 were 9.3 ± 0.9 mm and 7.4 ± 0.1 mm, respectively. The inhibitory zone diameter of medium extracts. Penicillium sp. R1M against S. aureus and E. coli were correspondingly 7.5 ± 0.1 mm and 7.1 ± 0.8 mm, while the medium extracts of P. notatum ATCC 28089 were 9.3 ± 0.6 mm and 7.4 ± 0.4 mm, respectively. Mycelial extracts of Penicillium sp. R1M showed better antibacterial activity than those of P. notatum ATCC 28089. On the other hand the medium extracts showed the opposite results.Keywords: antibacterial, Penicillium sp. R1M, Sonneratia caseolaris

Identifikasi Molekuler Bakteri Endofit Mangrove Rizhopora mucronata Penghasil Gelatinase (MMP2) Happy Nursyam; Asep Awaludin Prihanto
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 1 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Gelatinase adalah salah satu jenis keberagaman dari kelompok protease. Enzim metallo-endopeptidase ekstraseluler atau metaloproteinase mampu menghidrolisis gelatin. Enzim gelatinase menghidrolisis gelatin menjadi hidrolisat gelatin. Penelitian ini bertujuan mengisolasi bakteri endofit yang menghasilkan enzim gelatinase yang dapat dimanfaatkan untuk keperluan industri. Penelitian ini dilakukan dengan beberapa tahapan yaitu pengambilan dan preparasi sampel, skrinning gelatinase, seleksi strain potensial penghasil enzim gelatinase, dan analisis spesies menggunakan sekuensing 16S rDNA spesies bakteri. Hasil penelitian menunjukkan bahwa isolat dari bagian daun mangrove menunjukkan aktivitas enzim gelatinase yang tinggi dengan kode isolat CL-h4. Analisis sekuen 16S rDNA menunjukkan isolat memiliki kemiripan dengan Enterobacter hormaechei N6. Pohon filogenetik menunjukkan bakteri Enterobacter sp. UB-R** berada pada satu cabang maupun node (genus) yang sama dengan bakteri Enterobacter sp. dengan nilai boostrap sebesar0,6. Mikroorganisme endofit mangrove penghasil enzim gelatinase (MMP2) dari daun mangrove Rizhopora mucronata adalah strain baru Enterobacter sp. UB-R.

Isolation and Molecular Characterization of Gelatinase-Producing Bacteria from Mangrove Sediment Asep Awaludin Prihanto; Hidayatun Muyasyaroh; Abdul Aziz Jaziri; Nada Itorul Umam
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.10826

Protease is an important enzyme widely produced by microorganisms applied in food, health, and industry. Mangrove ecosystem, a rich microorganism habitat, accounted as a new resource for isolating the proteolytic bacteria. The purpose of this study was to identify protease-producing bacteria from mangrove ecosystems in the Tuban area, Indonesia. Three isolates that produced the gelatinase was successfully isolated from mangrove sediments. Bacterial isolates were then tested for extracellular gelatinase. The results showed that isolate T1 had high gelatinase activity. Two isolates (isolates T2 and T3) produced moderately gelatinase enzymes. Molecular identification revealed that isolate T1 is Enterobacter hormaechei.

Increase of Smoked-Fish plant income in Sumurgung, Tuban Regency Through the Introduction of equipment Production, Smoked-Fish with closed system. Asep Awaludin Prihanto, S.Pi., MP.
Journal of Innovation and Applied Technology Vol 5, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2020.005.02.13

Tuban Regency is one of the regencies in East Java that has abundant natural resources, especially in the field of fisheries. Catfish are quite popular among the people in Tuban Regency, but when the stock surplus or harvest is high, the selling price of catfish at the level of the community of farmers has decreased and resulted in decreased profits. Based on these conditions, we are optimizing the processing of catfish in order to increase the value of the benefits obtained by diversifying processed catfish products in the form of smoked catfish. Smoked fish has higher value because it can preserve fish and give a distinctive taste to the product. The purpose of this empowerment study is to compare income between diversifying and selling in fresh form.

Antimalarial Activity (Plasmodium falciparum 3D7) from Sea Sponge Acanthella sp. faidzil Ikram; Asep Awaludin Prihanto; Choiriyatun Hanifah; Muhammad Iqbal
Natural B, Journal of Health and Environmental Sciences Vol 1, No 3 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

The objective of the research was to get the bioactive candidates from marine sponge Acanthella sp. for anti multi resistant malaria. The first Research was conducted with isolating the active compounds of Acanthella sp. In vitro antimalarial test against Plasmodium falciparum 3D7 was done with several concentrations with positive control using chloroquinon the quantification of the Scizon inhibition was performed with blood staining. Afterward, the infected erythrocytes per 1000 erythrocytes was calculated. IC50 was assayed with probit analysis. The result indicated that all four active fractions showed inhibition on growth of P. Falciparum with different value on IC50. The best fraction is confirmed by fraction B with IC50 of 0.013 ppm.

BAKTERI SELULOLITIK SERASAH DAUN MANGROVEDI PULAU BANGKA Ardiansyah Kurniawan; Asep Awaludin Prihanto; Suci Puspitasari; Andi Kurniawan; Euis Asriani; Abu Bakar Sambah
Samakia : Jurnal Ilmu Perikanan Vol 9 No 1 (2018): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

The study aimed to obtain isolate of cellulolytic bacteria from leaf litter on mangrove in Bangka Island. Sampling was conducted on mangroves in Sungailiat, Bangka and Tukak Sadai, South Bangka district. The isolation was carried out using 1% enriched agarmedia of Carboxymetyl Cellulose (CMC). The bacterial isolates were tested with cellulolytic growth on 1% enriched CMC agar medium and lugol added at 72 hours. The clear zone resultingindicates cellulose degradation ability. The positive isolate of cellulolyticwas identified by biochemical tests. 1 of 5 isolates in Sungailiat positive cellulolytic mangroves and identified as Bacillus subtilis. 2 of 5 isolates in mangrove Tukak Sadai is positive as a cellulolytic bacteria and identified as Staphylococcus saproviticus and Bacillus cereus.

BAKTERI SELULOLITIK SERASAH DAUN MANGROVEDI PULAU BANGKA Ardiansyah Kurniawan; Asep Awaludin Prihanto; Suci Puspitasari; Andi Kurniawan; Euis Asriani; Abu Bakar Sambah
Samakia : Jurnal Ilmu Perikanan Vol 8 No 2 (2017): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Molecular Identification of Cellulolytic Bacteria From Mangrove Sediment at Tin Minning Region In West Bangka Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; andi kurniawan; abu bakar sambah; asep awaludin prihanto
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5848

Cellulose as an abundant source of glucose in Indonesia requires acceleration of decomposition utilizing cellulolytic bacteria. Cellulolytic bacteria can be obtained from the isolation of mangrove organic matter, such as sediments. Muntok Sub-district is one of the regions with the most tin mining in West Bangka Regency also has mangroves in the coastal area. Exploration of cellulolytic bacteria in mangroves with different environmental characteristics encourages researchers to find new bacterial strains that produce cellulase enzymes with new properties. Thirteen isolates were successfully isolated from three locations. Tembelok mangrove sediments produced Seven bacterial isolates, Peltim Mangrove samples produced three isolates and from Sukal Mangrove three isolates were obtained. Seven isolates showed clear zones in the Lugol test and three isolates including were gram-positive bacteria. Molecular test with 16S rRNA analysis showed TBL1 isolate has 85% similar identity of Vibrio parahaemolyticus strain HY3 and TBL2 isolate has 98% similar identity of Bacillus amyloliquefaciens strain HS8. Bacillus amyloliquefaciens potential to further study as cellulose degrading bacteria for feed ingredients.

Co-Authors Abdul Aziz Jaziri Abu Bakar Sambah Abu Bakar Sambah Affan Gaffar Alifatul Fatchiyah Andi Kurniawan Andi Kurniawan Andi Kurniawan Angga Wira Perdana Anies Chamidah Anif Fatwa Chawa Anisa Millah Taqiyyah Anisa Millah Taqiyyah Ardiansyah Kurniawan Choiriyatun Hanifah Dwi Febrianti Euis Asriani Euis Asriani Euis Asriani faidzil Ikram Farikh Assafri Gilang Drajat Maulana Guntur Guntur Hanan Dwi Laksono Timur Happy Nursyam Hardoko Hardoko Hartati Kartikaningsih Hidayatun Muyasharoh Hidayatun Muyasyaroh Hidayatun Muyasyaroh Hidayatun Muyasyaroh Jauharotul Afifah Karimah Karimah Kartika D. Aninta Ken Audia Pradarameswari Ken Audia Pradarameswari Ken Audia Pradarameswari M. Aris Munandar Maftuch Maftuch Marlinda Elvina Susanti MOHAMAD FADJAR Mohammad Fadjar Muhamad Firdaus Muhammad Firdaus Muhammad Iqbal Nada Itorul Umam Rahmi Nurdiani Randy Fahrudin Ardiansyah Soffi Trisnaningrum Suci Puspita Sari Suci Puspita Sari Suci Puspita Sari Suci Puspitasari Sukoso Sukoso Titis Sari Kusuma Uun Yanuhar Wenny Bekti Sunarharum Yenny Risjani Yoga Dwi Jatmiko

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