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Pemanfaatan Mangrove Rhizophora mucronata Sebagai Pewarna Alami Kain Katun Dewi, Lutfianna Fatma; Pringgenies, Delianis; Ridlo, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (684.945 KB) | DOI: 10.14710/jmr.v7i2.25896

Abstract

ABSTRAK : Bagian-bagian pohon dari R. mucronata seperti serasah daun, kulit kayu, maupun limbah propagul diketahui memiliki kandungan pewarna yang ramah lingkungan. Potensi pewarna alami dari tumbuhan ini dapat menjadi alternatif bahan produksi bagi industri batik di Indonesia. Pengambilan sampel dilakukan di Teluk Awur, Jepara, Jawa Tengah pada bulan Oktober 2015 kemudian dilakukan ekstraksi pewarna, pewarnaan, dan berbagai analisis. Tujuan penelitan adalah untuk mengetahui kualitas pewarna alami dari bagian-bagian pohon R. mucronata sebagai pewarna alami pada kain katun. Sampel R. mucronata yang diambil yaitu kulit kayu, limbah propagul, dan serasah daun. Ekstraksi pewarna dilakukan menggunakan air panas pada suhu 30°C, 50°C, dan 70°C. Pengikatan warna setelah pencelupan menggunakan kain katun mori primissima dengan mordan tawas. Hasil penelitian menunjukkan bahwa warna cokelat dihasilkan oleh ekstrak R. mucronata. Analisis FTIR dan UV Vis mengindikasikan adanya senyawa tanin terkondensasi. Pengujian Total Phenol Content (TPC) dan Total Flavonoid Content (TFC) menunjukkan hasil positif sebesar 2,4950 mg GAE/g untuk TPC dan 0,6516 mg QE/g untuk TFC. Hasil pindaian dengan Scanning Electron Microscope menunjukkan granula-granula yang pecah pada spesimen. Kualitas pewarnaan pada kain menunjukkan kisaran hasil antara 3 (cukup) hingga 4 (baik) dan telah memenuhi standar SNI. Pewarna dari serasah daun pada ekstraksi 70°C menunjukkan hasil terbaik di antara yang lain. ABSTRACT : The tree parts of R. mucronata such as the leaves litter, bark, and propagule are known for containing dyes that are environmentally friendly. This natural dye potential could be used for the production resource alternative for the batik industry in Indonesia. Sampling was conducted in Teluk Awur, Jepara, Central Java on October 2015 and followed by dye extraction, dying process, and also several analysis. The purpose of this research was to discover the quality of natural dye from R. mucronata on cotton fabric. Samples that were taken from R. mucronata were bark, leaves litter, and propagule litter. Dye extraction used hot water method with variants of temperature: 30°C, 50°C, dan 70°C. The color locking after dyeing used cotton fabric with mori primissima type and alum as the mordant. The obtained result from extract R. mucronata showed brown color. Condensed tannin was indicated from FTIR and UV Vis analysis. Total Phenol Content and Total Flavonoid Content assays showed positive result as follows: 2,4950 mg GAE/g for TPC and 0,6516 mg QE/g for TFC. Scanning result using Scanning Electron Microscope showed that the granules break on the specimen. Color fastness quality showed the range of result from colored fabric from 3 (enough) until 4 (well) and already fulfilled SNI standard. The dye extracted from leaves litter in 70°C showed the best result among the others. 
KAJIAN AKTIVITAS BIOAKTIF EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP JAMUR Candida albicans Pranoto, Eunike Noviana; Ma'ruf, Widodo Farid; Pringgenies, Delianis
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 1, No 2 (2012): Jurnal Pengolahan dan bioteknologi hasil perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.471 KB)

Abstract

Teripang dapat ditemukan di perairan Indonesia. Teripang diketahui memiliki zat yangsalah satunya berfungsi sebagai antijamur. Penyakit infeksi pada manusia yangdisebabkan oleh jamur Candida albicans di Indonesia masih relatif tinggi dan obatantijamur relatif lebih sedikit dibandingkan dengan antibakteri. Pengobatan terhadap C.albicans secara kimia dapat menimbulkan resistensi dan efek samping. Penelitian inibertujuan untuk mengetahui pengaruh penggunaan pelarut dan konsentrasi yang berbedadari ekstrak Holothuria scabra terhadap C. albicans serta mengetahui kandungansenyawa bioaktif dalam H. scabra. Hasil penelitian didapatkan bahwa pelarut terbaikuntuk mengisolasi senyawa antijamur H. scabra adalah metanol. Konsentrasi ekstrak 5mg/ml, 6 mg/ml,7 mg/ml menghasilkan zona hambat berturut-turut sebesar 5,1 + 0,56mm; 8,42 + 0,71 mm; 10,1 + 0,59 mm. Hasil uji kandungan bioaktif memperlihatkanbahwa ekstrak H. scabra mengandung senyawa alkaloid, saponin, steroid dantriterpenoid. Ada indikasi potensi antijamur pada ekstrak H. scabra.
Karakterisasi Metabolit Sekunder Bakteri Simbion Gastropoda Conus miles dengan Metode GC-MS Sebagai Antibakteri MDR (Multi Drug Resistant) Hasanah, Nurul Fitrah; Pringgenies, Delianis; Wulandari, Sri Yulina
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.74 KB) | DOI: 10.14710/jmr.v1i2.2038

Abstract

It is known that the bacterial symbionts are symbiotic with gastropods Conus miles has the potential to be able to produce antibacterial compounds against bacterial MDR (Multi Drug Resistant). Information found previously from bacterial symbionts and isolation based on screening results, the size of the zone of inhibition and inhibitory properties against several types of bacteria tests showed that isolates derived from Conus miles Gastropoda symbionts characterized by kinship rate of 98% in bacteria Pseudoalteromonas sp. The research objective was to determine the characteristics of the bacterial symbiont bioactive compounds associated with the type of mollusk Conus miles. The study was conducted three phases namely the isolation and purification of bacteria; characterization of isolates identified through test results on the reaction of physiological and biochemical tests, as well as the identification of secondary metabolites by GC-MS method QP2010S Shimadzu. The results showed that the bacterial symbiont isolates had a number of compounds with a percentage of the highest peak to the lowest compound the compound, Acetic acid (CAS) ethylic acid (35.22%); propanoic acid, 2-methyl-(CAS) Isobutyric acid (11.78%); Iso -valeric acid (9:38%), butanoic acid, 2-methyl-(CAS) 2-methylbutanoic acid (4.74%) and 1,2-Benzenedicarboxylic acid, dioctyl ester (CAS) Dioctyl phthala (4:36%). The results concluded that the bacterial symbiont isolates obtained in this study provide information on the results of potentially new antibiotics.
Uji Fitokimia Dan Toksisitas Ekstrak Kasar Gastropoda (Telescopium telescopium) Terhadap Larva Artemia salina Putri, Mukti K. Diana; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.912 KB) | DOI: 10.14710/jmr.v1i2.2020

Abstract

Gastropod is one of the marine organisms that can be used as a source of natural bioactive compounds, one of which is Telescopium telescopium. T. telescopium until now it has not been yet exploited optimally, some people still use it as feed ingredients that contain high protein nutrition, however it must be supported by scientific information about the efficacy and side effects. This research aims at determining the secondary metabolite by crude extract T. telescopium, and the lethal toxicity value (LC50-24 hours). The research consisted of two steps, namely the phytochemical and toxicity test. The method used exploratif and experimental laboratory with a complete randomized design, which consited of five concentration treatments namely 25.12, 63.1, 158.5, 398.15, and 1000 ppm with three replications. Each treatment used 10 Artemia salina larvae, and observations were made during 24 hours of Artemia salina larvae mortality. LC50 was determined by probit analysis using the EPA Probit Analysis Program Version 1.5 Finney. The results of the phytochemical test showed that crude extract T. telescopium contain alkaloid compounds, steroids, flavonoids. The results BSLT (Brine Shrimp Lethality Test) of crude extract T. telescopium LC50-24 hours showed that the crude extract clorofom (229.562 ppm), crude extract ethyl acetate (244.906 ppm), and crude extract methanol (197.242 ppm). LC50-24 hours < 1000 ppm showed that the crude extract Telescopium telescopium potent antitumor compounds.
KAJIAN AKTIVITAS BIOAKTIF EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP JAMUR Candida albicans Pranoto, Eunike Noviana; Ma'ruf, Widodo Farid; Pringgenies, Delianis
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 1, No 1 (2012): Jurnal Pengolahan dan bioteknologi hasil perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.502 KB)

Abstract

Sea cucumbers are found in Indonesian oceans. Sea cucumbers have been known containing substances as antifungal. Infection diseases in human caused by fungi, such as Candida albicans, are still high and antifungal drugs are less than antibacterial drugs in amount. Medical curing of Candidiasis chemically can use chemical agents. But they can caused resistance and side effects. The purpose of this research is to know the influence of different solvent and concentration usage of Holothuria scabra extract towards C. albicans and to know the bioactive substances contained in H. scabra. The results obtained from this research were: the best solvent to extract antifungal agents from H. scabra is methanol. Extract concentration of 5 mg/ml, 6 mg/ml,7 mg/ml showed inhibition zone continuously as high as 5,1 + 0,56 mm; 8,42 + 0,71 mm; 10,1 + 0,59 mm. The results are significantly different (P < 0,01), different (0,01 < P < 0,05), and not different (P > 0,05) for antifungal activity test. Bioactive substance test showed that alkaloid, saponin, steroid, and triterpene were found in H. scabra. H. scabra extract indicates potential activity as antifungal agent.    
IDENTIFIKASI PIGMEN KAROTENOID PADA BAKTERI SIMBION KARANG Pocillopora damicornis Idris, Ryandha; Riniatsih, Ita; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.134 KB) | DOI: 10.14710/jmr.v3i3.5996

Abstract

Karotenoid merupakan pigmen merah, kuning dan orange yang diketahui memiliki aktivitas antioksidan. Karotenoid dapat ditemukan pada tanaman, hewan dan bakteri.  Penelitian bertujuan untuk mengidentifikasi pigmen karotenoid pada bakteri simbion karang Pocillopora damicornis. Identifikasi pigmen menggunakan Spektrofotometer UV-Vis pada panjang gelombang 190-800 nm dan Kromatografi Cair Kinerja Tinggi (KCKT) fase terbalik ODS/C18 dengan fase gerak metanol:asetonitril (7:3 v/v) pada panjang gelombang 190-800 nm. Uji DPPH dilakukan dengan metode diphenylpicrylhydrazil (DPPH) dan pengukuran absorbansi dilakukan pada panjang gelombang 517 nm. Identifikasi bakteri simbion karang Pocillopora damicornis dilakukan menggunakan metode PCR 16S rDNA. Hasil penelitian memperlihatkan bahwa dari 9 isolat bakteri simbion terdapat 1 isolat bakteri simbion karang Pocilllopora damicornis yang positif memiliki pigmen kaotenoid yaitu 5.A.4.Isolat 5.A.4 mengandung pigmen karotenoid Prasinoxanthin, Alloxanthin, Siphonein dan Crocoxanthin yang merupakan kelompok xantofil dan memiliki aktivitas peredaman radikal bebas DPPH sebesar 6,12%. Hasil identifikasi bakteri dengan metode PCR 16S rDNA menujukkan bahwa isolat bakteri 5.A.4. memiliki tingkat kekerabatan sebesar 99% dengan bakteri Bacillius subtilis.Hasil penelitian menunjukkan bahwa bakteri karang P. damicornis mempunyai potensi untuk dikembangkan sebagai sumber pigmen alami yang berasal dari laut dan berkelanjutan.
Uji Fitokimia dan Aktivitas Antijamur Ekstrak Teripang Keling (Holoturia atra) Dari Pantai Bandengan Jepara Terhadap Jamur Candida albicans Septiadi, Tedi; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.857 KB) | DOI: 10.14710/jmr.v2i2.2355

Abstract

Holothuria atra is one of the marine lifes that lives at the bottom of the substrate, and able to adapt to its environment. Several previous studies showed that extracts of H. atra from different waters have activity as an antifungal. Fungus Candida albicans is one of human pathogens that attack on the mucosa of the mouth, skin and vagina. The purpose of this study were to identify the compounds contained in the extracts of H.atra and examine the effect of extracts concentration against C. albicans. The process of extraction was done by maceration with solvent n-hexane, ethyl acetate and methanol. Testing of secondary metabolites was carried out using phytochemical screening methods while testing antifungal activity was using agar diffusion test. The results showed that the extracts of H.atra contained saponins, alkaloids, steroids and triterpenoids. Antifungal activity assays showed that the n-hexane extract did not show any inhibition zone, while the ethyl acetate and methanol extracts showed inhibition zone at a concentration of 1 mg / disk with a large zone of inhibition of 8.27 ± 0.06 and 8.07 ± 0 , 12 mm, respectively based on these results it can be concluded that the extract of ethyl acetate solvent H.atra has strong potential as antifungal.
Penapisan Bakteri Simbion Gastropoda Stramonita armigera Penghasil Senyawa Antibakteri Multi Drug Resistant dari Perairan Ternate Pringgenies, Delianis; Dananjoyo, Mijil Ciptaning
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.733 KB) | DOI: 10.31258/jnat.13.3.200-206

Abstract

Antibiotic resistance is an ability of bacteria to hold the antibiotic effect. It was reported that there is a human-patogen bacteria that resistance to one or more classes of antibiotic. It become a problem on medical world. Tosolve those problems, it is necessary to search the new antibiotic compounds that more effective and efficient tosolve the problem of Multi Drug Resistance (MDR). The secondary metabolite-producing marine invertebrates andsymbiont microorganisms, have prospect as an antibiotic. The symbiont microorganisms may produce thesecondary metabolite similar to their host. The aims of the reseach were to determinate of gastropods symbiontbacteria that capable of producing Antibacterial MDR (Multi Drugs Resistant) Compound. Sample of Molusc werecollected from Ternate (Molucas) islands. Isolation of symbiotic bacteria, screening for bacteria which producingsecondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria (MDR),conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplificationbased on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited usingGENETYX program and followed by 16S rDNA sequence analysis. The result showed that 17 strains were isolatedfrom gastropods Stramonita armigera. Antibacterial assays showed that TSA 8.7 isolate have ability to inhibitPseudomonas sp., Escherichia coli dan Enterobacter sp. the molecular analyses showed that isolate TSA 8.7closed by related to Vibrio sp. Strain JZDN1, with 98% of homology. Based on this experimental result, it could beconcluded that gastropods-symbiont bacterium Stramonita armigera capable of producing antibacterial compoundagainst strain Multi Drug Resistant (MDR). There is 11 isolates of gastropods-symbiont bacteria Stramonita armigerathat have an antibacterial MDR activity.
Potensi Antibakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus Siregar, Angelina Ferawaty; Sabdono, Agus; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.97 KB) | DOI: 10.14710/jmr.v1i2.2032

Abstract

Along with the increasing resistance of bacteria in the health world, it is necessary discover a new drug. Sources of new antibacterial bioactive compounds can be obtained from seaweeds. The purposes of the research were to determine the antibacterial activity of crude extract seaweed againts the skin disease bacteria, Pseudomonas aeruginosa, Staphylococcus epidermidis and Micrococcus luteus, and to identify classes of compounds contained in crude extract of seaweed. This research was done from January to Mei 2012. Seaweeds sampling (Caulerpa sp, Eucheuma sp, Gracilaria sp dan Sargassum sp) was conducted in territorial water of Jepara. Extraction process and analysis of bioactive compound of crude extracts was conducted at Laboratory, Marine Science Department, Diponegoro University, Semarang. Test antibacterial activity was conducted at Medicine Laboratory, Ungaran. The method used in this research was experimental laboratories. Extraction process was conducted with solid-liquid extraction method. Extract were tested for antibacterial activity by agar diffusion method according to Kirby-baurer. Based on the antibacterial activity’s assay, 12 extracts which have antibacterial activity against test bacteria P. aeruginosa, S. epidermidis and M. luteus were obtained the 12 seaweed extracts the highest consentration of inhibition zone was 200 µg/disk in ethyl acetate extract of the Sargassum sp was the most active extracts against bacteria P. aeruginosa and M. luteus, while the methanol extract of Sargassum sp was the most active extracts against the bacteria S. epidermidis. Steroids was the most dominant compounds on all of 12 seaweed extracts.
Aktivitas Antioksidan dan Kandungan Total Fenolik Pada Teripang di Perairan Karimunjawa, Jepara Avigail, Yolanda; Yudiati, Ervia; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.442 KB) | DOI: 10.14710/jmr.v8i4.24600

Abstract

Teripang merupakan hewan jenis Echinodermata. Beberapa species teripang, misalnya H. scabra, H. fuscogilva dan T. ananas memiliki nilai jual yang tinggi. Meskipun tidak semua spesies memiliki nilai jual yang tinggi, teripang berpotensi untuk dikembangkan dalam bidang kesehatan, terutama dalam kemampuannya untuk meredam radikal bebas dan mencegah berbagai penyakit degeneratif. Tujuan dari penelitian ini yaitu untuk mengidentifikasi, menentukan aktivitas antioksidan, kandungan total fenol, serta kadar karotenoid pada ekstrak beberapa teripang yang berasal dari Perairan Karimunjawa Jepara. Hasil identifikasi dari ke empat spesies adalah Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, Bohadschia vitiensis, dan Holothuria atra. Ekstraksi dilakukan dengan teknik maserasi dinding tubuh teripang menggunakan pelarut metanol. Uji aktivitas antioksidan dilakukan dengan menggunakan metode DPPH (1,1-difenil-pikrilhidrazil) sebagai agen radikal bebas. Penentuan total fenolik dilakukan menggunakan metode folin-ciocalteu, dan penentuan kadar karotenoid dilakukan dengan menggunakan spektrofotometer. Hasil menunjukkan bahwa aktivitas antioksidan Bohadschia vitiensis, Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, dan Holothuria atra tergolong sangat lemah dengan nilai IC50 secara berturut-turut adalah sebesar 454,28 ± 14,4; 713,51 ± 9,1; 801,57 ± 8,4 dan 1535,47 ppm. Kandungan total fenol sebesar 21,08 ± 0,49; 14,325 ± 0,21; 14,033 ± 0,33 dan 10,67 ± 0,12 mg GAE/g sampel, dan kadar karotenoidnya sebesar 23,28 ± 0,07; 25,78 ± 0,56; 11,85 ± 0,04; dan 41,44 ± 0,008 µmol/g sampel.Kata kunci: Teripang, Antioksidan, DPPH, Fenolik, Karotenoid Sea cucumbers are sea benthos from phylum Echinoderms phylum. Some sea cucumbers such as H. scabra, H. fuscogilva, T. Ananas have a high economical price. Eventhough, some sea cucumbers have the potency of being a healthy food. It it scientifically proven that some sea cucumbers have the ability to reduce free radicals and prevent various degenerative diseases caused by the excessive free radicals. The aims of this study were to identified, determine antioxidant activity, total phenol content, and carotenoid levels in sea cucumber extracts originally from Karimunjawa waters. Some of the sea cucumbers obtained are Stichopus cf. quadrifasciatus, Pearsonothuria graeffei, Bohadschia vitiensis, and Holothuria atra. The extraction was done by macerating the body walls with methanol. The antioxidant activity test were tested using the DPPH method as free radicals, the total phenolic content tested using the folin-ciocalteu method, and determined the carotenoid levels spectrophotometrically. Based on the results, antioxidant activity of  B. vitiensis, S. cf. quadrifasciatus, P. graeffei, and H. atra were  classified as very weak with IC50 454.28 ± 14.4; 713.51 ± 9.1; 801.57 ± 8.4 dan 1535.47 ppm. Total phenolic content were 21.08 ± 0.49; 14.325 ± 0.21; 14.033 ± 0.33 dan 10.67 ± 0.12 mg GAE / g samples, while carotenoid levels were 23.28 ± 0.07; 25.78 ± 0.56; 11.85 ± 0.04; dan 41.44 ± 0.008 µmol / g sample.Keyword : Sea cucumber, Antioxidant, DPPH, Phenolic, Carotenoid