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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Natur Indonesia Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Ilmiah Perikanan dan Kelautan Journal of Aquatropica Asia Jurnal Kelautan Tropis Panrita Abdi - Jurnal Pengabdian pada Masyarakat JFMR (Journal of Fisheries and Marine Research) JRST (Jurnal Riset Sains dan Teknologi) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengelolaan Perairan Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Natural Pigments Aquacultura Indonesiana Journal of Tropical Marine Science Indonesian Journal of Mollusk Journal of Marine Research JURNAL BIOLOGI PAPUA

Delianis Pringgenies

Jurusan Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro Jl. H. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275

Author-ID : 347619

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Other

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Free Radicals Scavenging Activities of Low Molecular Weight Sodium Alginate (LMWSA) from Sargassum polycystum, Produced by Thermal Treatment Yudiati, Ervia; Pringgenies, Delianis; Djunaedi, Ali; Arifin, Zaenal; Sudaryono, Agung
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

In this study, the effects of alginate from Sargassum polycystum molecular reduction by thermal heating on DPPH anti radical scavenging activity were investigated. Raw alginate as the control treatment was heated at 140oC in a laboratory oven for different time courses 1.5, 4.5, and 7.5 hours. The assessment of molecular weight, UV-visible and FT-IR spectroscopic studies were applied. By heat treatment, molecular weight of polymer was decreased in a time-dependent manner, though there is no significant difference between 4.5 h and 7.5 h samples. The UV-visible spectroscopic studies pointed that there was a new absorption band between 250 and 290 nm in alginate heated treatments. The higher antiradical scavenging activity were reached from 1.5 h and 4.5 h treatments (19.83% and 20.07%). Interestingly, the antiradical scavenging activity of the longest heating treatment (7.5 h) was reduced (16.85%), similar to the raw alginate (17.89%). Prolonged heat treatments influenced the antioxidant activity and reduced the ability of donate electrons or hydrogen atoms to inactivate this radical action.

The antioxidant activity of carotenoid pigments in the bacterial symbionts of seagrass Syringodium isoetifolium Pringgenies, Delianis; Idris, Riyada
Indonesian Journal of Natural Pigments Vol 1 No 1 (2019): February 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.1.12

Carotenoids are pigments of red, yellow and orange which are found in plants, animals and bacteria, and are known to have antioxidant activity. This study aims to identify the carotenoid pigments detected in seagrass Syringodium isoetifolium bacterial symbionts. Isolation of bacteria was conducted using dispersive media Zobell 2116E. Bacterial isolates were cultured and then centrifuged at 8000 rpm for 10 minutes and extracted using methanol. Identification of the pigment was done by using High Performance Liquid Chromatography (HPLC) reversed phase ODS / C18. The mobile phase was carried out using a mixture of methanol: acetonitrile (7: 3 v / v). Free radical reduction activities determined by the method of DPPH (diphenylpicrylhydrazil) and its absorbance was measured at a wavelength of 517 nm. Identification of the bacterial symbionts from the seagrass S. isoetifolium performed using 16S rDNA PCR method. The results showed that, of the 12 bacterial isolates obtained, isolate 7A was proven to contain caratenoid pigment. Pigment extracts of the bacterial isolates had free radical DPPH reduction activity of 40.4%. The results showed that the identification of bacteria isolates 7A had 100% level of kinship with the bacteria Bacillus amyloliquifaciens.

FENOMENA BIOLUMINENSENSI CUMI-CUMI (Loligo duvauceli) BERASAL DARI BAKTERI SIMBION Pringgenies, Delianis
Jurnal Harpodon Borneo Vol 5, No 1 (2012): Volume 5 No 1 April 2012
Publisher : Jurnal Harpodon Borneo

Tujuan penelitian adalah mengungkap simbiosis antara bakteri dengan organ cahaya cumi-cumi pada proses bioluminesensi dengan asumsi bahwa: kemampuan pencahayaan tidak dimiliki oleh organisme cumi-cumi itu sendiri; terjadi simbiosis antara cumi-cumi dan bakteri; dan proses simbiosis antara bakteri dengan cumi-cumi terjadi secara horizontal, yakni bakteri berasal dari luar dan besimbiosis pada cumi-cumi setelah menetas dari telur. Pemecahan masalah dalam penelitian ini dilakukan melalui aspek cumi-cumi dan bakteri, dan kemudian dianalisis tentang ketergantungan kedua organisme tersebut dengan cara: histologi organ sebagai sumber cahaya dengan metode mikroskop elektron transmisi (TEM ) pada juvenil dan dewasa cumi-cumi. Hasil penelitian menunjukkan bahwa jenis cumi-cumi yang memancarkan cahaya memiliki sepasang organ cahaya menempel pada dorso-lateral kantung tinta. Organ cahaya sudah terbentuk sempurna pada cumi-cumi juvenil umur satu hari dan selanjutnya membesar mengikuti pertumbuhan cumi-cumi. Bakteri di dalam kantung bakteri merupakan bakteri yang hidup secara ektraselular dan kerapatannya di dalam kantung meningkat sejalan dengan umur cumi-cumi juvenil sampai dewasa. Dari hasil analisis histologi kantung organ cahaya, didapat bahwa kantung organ cahaya cumi-cumi juvenil umur satu hari belum mengandung bakteri. Bakteri mulai dijumpai dalam koloni kecil pada cumi-cumi umur dua hari dan pada cumi-cumi yang lebih besar serta dewasa. Sel-sel bakteri terdapat dalam kerapatan yang padat di dalam lumen kantung hewan dewasa. Kehadiran bakteri pada organ cahaya cumi-cumi terjadi secara horizontal yang berarti bahwa bakteri berasal dari luar lalu masuk bersimbiosis ke dalam organ cahaya melalui saluran bersilia kantung setelah cumi-cumi menetas. Bioluminesensi yang terjadi dimanfaatkan cumi-cumi untuk strategi pertahanan melalui penyamaran dan mekanisme komunikasi antar lawan jenis. Key words : Bakteri, simbion, cumi, organ cahaya

Penapisan Bakteri Simbion Gastropoda Stramonita armigera Penghasil Senyawa Antibakteri Multi Drug Resistant dari Perairan Ternate Pringgenies, Delianis; Dananjoyo, Mijil Ciptaning
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Antibiotic resistance is an ability of bacteria to hold the antibiotic effect. It was reported that there is a human-patogen bacteria that resistance to one or more classes of antibiotic. It become a problem on medical world. Tosolve those problems, it is necessary to search the new antibiotic compounds that more effective and efficient tosolve the problem of Multi Drug Resistance (MDR). The secondary metabolite-producing marine invertebrates andsymbiont microorganisms, have prospect as an antibiotic. The symbiont microorganisms may produce thesecondary metabolite similar to their host. The aims of the reseach were to determinate of gastropods symbiontbacteria that capable of producing Antibacterial MDR (Multi Drugs Resistant) Compound. Sample of Molusc werecollected from Ternate (Molucas) islands. Isolation of symbiotic bacteria, screening for bacteria which producingsecondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria (MDR),conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplificationbased on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited usingGENETYX program and followed by 16S rDNA sequence analysis. The result showed that 17 strains were isolatedfrom gastropods Stramonita armigera. Antibacterial assays showed that TSA 8.7 isolate have ability to inhibitPseudomonas sp., Escherichia coli dan Enterobacter sp. the molecular analyses showed that isolate TSA 8.7closed by related to Vibrio sp. Strain JZDN1, with 98% of homology. Based on this experimental result, it could beconcluded that gastropods-symbiont bacterium Stramonita armigera capable of producing antibacterial compoundagainst strain Multi Drug Resistant (MDR). There is 11 isolates of gastropods-symbiont bacteria Stramonita armigerathat have an antibacterial MDR activity.

KAJIAN AKTIVITAS BIOAKTIF EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP JAMUR Candida albicans Pranoto, Eunike Noviana; Ma'ruf, Widodo Farid; Pringgenies, Delianis
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 1, No 2 (2012): Jurnal Pengolahan dan bioteknologi hasil perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.471 KB)

Teripang dapat ditemukan di perairan Indonesia. Teripang diketahui memiliki zat yangsalah satunya berfungsi sebagai antijamur. Penyakit infeksi pada manusia yangdisebabkan oleh jamur Candida albicans di Indonesia masih relatif tinggi dan obatantijamur relatif lebih sedikit dibandingkan dengan antibakteri. Pengobatan terhadap C.albicans secara kimia dapat menimbulkan resistensi dan efek samping. Penelitian inibertujuan untuk mengetahui pengaruh penggunaan pelarut dan konsentrasi yang berbedadari ekstrak Holothuria scabra terhadap C. albicans serta mengetahui kandungansenyawa bioaktif dalam H. scabra. Hasil penelitian didapatkan bahwa pelarut terbaikuntuk mengisolasi senyawa antijamur H. scabra adalah metanol. Konsentrasi ekstrak 5mg/ml, 6 mg/ml,7 mg/ml menghasilkan zona hambat berturut-turut sebesar 5,1 + 0,56mm; 8,42 + 0,71 mm; 10,1 + 0,59 mm. Hasil uji kandungan bioaktif memperlihatkanbahwa ekstrak H. scabra mengandung senyawa alkaloid, saponin, steroid dantriterpenoid. Ada indikasi potensi antijamur pada ekstrak H. scabra.

KAJIAN AKTIVITAS BIOAKTIF EKSTRAK TERIPANG PASIR (Holothuria scabra) TERHADAP JAMUR Candida albicans Pranoto, Eunike Noviana; Ma'ruf, Widodo Farid; Pringgenies, Delianis
Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Vol 1, No 1 (2012): Jurnal Pengolahan dan bioteknologi hasil perikanan
Publisher : Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.502 KB)

Sea cucumbers are found in Indonesian oceans. Sea cucumbers have been known containing substances as antifungal. Infection diseases in human caused by fungi, such as Candida albicans, are still high and antifungal drugs are less than antibacterial drugs in amount. Medical curing of Candidiasis chemically can use chemical agents. But they can caused resistance and side effects. The purpose of this research is to know the influence of different solvent and concentration usage of Holothuria scabra extract towards C. albicans and to know the bioactive substances contained in H. scabra. The results obtained from this research were: the best solvent to extract antifungal agents from H. scabra is methanol. Extract concentration of 5 mg/ml, 6 mg/ml,7 mg/ml showed inhibition zone continuously as high as 5,1 + 0,56 mm; 8,42 + 0,71 mm; 10,1 + 0,59 mm. The results are significantly different (P < 0,01), different (0,01 < P < 0,05), and not different (P > 0,05) for antifungal activity test. Bioactive substance test showed that alkaloid, saponin, steroid, and triterpene were found in H. scabra. H. scabra extract indicates potential activity as antifungal agent. Â Â

IDENTIFIKASI PIGMEN KAROTENOID PADA BAKTERI SIMBION KARANG Pocillopora damicornis Idris, Ryandha; Riniatsih, Ita; Pringgenies, Delianis
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Karotenoid merupakan pigmen merah, kuning dan orange yang diketahui memiliki aktivitas antioksidan. Karotenoid dapat ditemukan pada tanaman, hewan dan bakteri.Â Penelitian bertujuan untuk mengidentifikasi pigmen karotenoid pada bakteri simbion karang Pocillopora damicornis. Identifikasi pigmen menggunakan Spektrofotometer UV-Vis pada panjang gelombang 190-800 nm dan Kromatografi Cair Kinerja Tinggi (KCKT) fase terbalik ODS/C18 dengan fase gerak metanol:asetonitril (7:3 v/v) pada panjang gelombang 190-800 nm. Uji DPPH dilakukan dengan metode diphenylpicrylhydrazil (DPPH) dan pengukuran absorbansi dilakukan pada panjang gelombang 517 nm. Identifikasi bakteri simbion karang Pocillopora damicornis dilakukan menggunakan metode PCR 16S rDNA. Hasil penelitian memperlihatkan bahwa dari 9 isolat bakteri simbion terdapat 1 isolat bakteri simbion karang Pocilllopora damicornis yang positif memiliki pigmen kaotenoid yaitu 5.A.4.Isolat 5.A.4 mengandung pigmen karotenoid Prasinoxanthin, Alloxanthin, Siphonein dan Crocoxanthin yang merupakan kelompok xantofil dan memiliki aktivitas peredaman radikal bebas DPPH sebesar 6,12%. Hasil identifikasi bakteri dengan metode PCR 16S rDNA menujukkan bahwa isolat bakteri 5.A.4. memiliki tingkat kekerabatan sebesar 99% dengan bakteri Bacillius subtilis.Hasil penelitian menunjukkan bahwa bakteri karang P. damicornis mempunyai potensi untuk dikembangkan sebagai sumber pigmen alami yang berasal dari laut dan berkelanjutan.

Uji Fitokimia dan Aktivitas Antijamur Ekstrak Teripang Keling (Holoturia atra) Dari Pantai Bandengan Jepara Terhadap Jamur Candida albicans Septiadi, Tedi; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Holothuria atra is one of the marine lifes that lives at the bottom of the substrate, and able to adapt to its environment. Several previous studies showed that extracts of H. atra from different waters have activity as an antifungal. Fungus Candida albicans is one of human pathogens that attack on the mucosa of the mouth, skin and vagina. The purpose of this study were to identify the compounds contained in the extracts of H.atra and examine the effect of extracts concentration against C. albicans. The process of extraction was done by maceration with solvent n-hexane, ethyl acetate and methanol. Testing of secondary metabolites was carried out using phytochemical screening methods while testing antifungal activity was using agar diffusion test. The results showed that the extracts of H.atra contained saponins, alkaloids, steroids and triterpenoids. Antifungal activity assays showed that the n-hexane extract did not show any inhibition zone, while the ethyl acetate and methanol extracts showed inhibition zone at a concentration of 1 mg / disk with a large zone of inhibition of 8.27 Â± 0.06 and 8.07 Â± 0 , 12 mm, respectively based on these results it can be concluded that the extract of ethyl acetate solvent H.atra has strong potential as antifungal.

Uji Toksisitas Ekstrak Kloroform Cangkang dan Duri Landak Laut (Diadema setosum) Terhadap Mortalitas Nauplius Artemia sp Aprilia, Hilda Ayu; Pringgenies, Delianis; Yudiati, Ervia
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Eggshells and spines of sea urchins (Diadema setosum) have toxins that can lead to certain biota surrounding death. Presumably the sea urchin has toxic compounds that can affect the surrounding environment. Because it needed a way to determine the toxic properties of sea urchin. Bioactive compounds contained in a previous study showing shells and spines of certain species of sea urchin pigment is coated by a black liquid that is stable. This fluid can be used as a mesh and skin coloring. It can also be used as fertilizer. This study aims to determine the lethal toxicity (LC50-24 h) of the chloroform extract of sea urchin of mortality Nauplius Artemia sp. The method used in this study is the experimental laboratory. Chloroform extract of the eggshells and sea urchin spines were tested using animal toxicity tests Nauplius Artemia sp.The results showed that the lethal toxicity (LC50-24 h) of the Nauplius Artemia sp in the chloroform extract of sea urchin eggshells at 133.58 ppm. As for the chloroform extract of sea urchin spines obtained results for 168.178 ppm. Chloroform extracts of both eggshells and sea urchin spines have potential activity as anti-microbial bio

Uji Aktivitas Antioksidan Ekstrak Kasar Keong Bakau (Telescopium telescopium) dengan Pelarut yang Berbeda terhadap Metode DPPH (Diphenyl Picril Hidrazil) Rahmayani, Ulfah; Pringgenies, Delianis; Djunaedi, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Mangrove snail (Telescopium telescopium) is one of the marine gastropod and some communities use as foodstuff. The purpose of study was to find out the antioxidant activity by crude extract of mangrove snails (T. telescopium) using DPPH method in different solvents. The method consists of extraction using gradient solvent (chloroform, ethyl acetate and methanol), phytochemical test and antioxidant activity test using DPPH method. DPPH test using a series of concentration of 100 ppm, 200 ppm, 400 ppm and 800 ppm with triplicate repetition. IC50 values were determined by calculating the regression analysis % inhibition against the concentration of crude extract. The crude extract of mangrove snails is contained three bioactive components in the form of alkaloids, steroids and flavonoids.The results showed that the IC50 value of chloroform, ethyl acetate and methanol extract were 47274.00 ppm, 4143.58 ppm and 2329.81 ppm, respectively. The IC50 values of all crude extract have a very weak antioxidant activity (IC50 > 200 ppm), with IC50 of BHT as positive control was 4.91 ppm.

Co-Authors Adityo Harmawan Adityo Harmawan Agung Sudaryono Agus Sabdono Agus Trianto Aiyen Tjoa Alfi Satriadi Alfin Anggraeni Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridho Ali Ridho Ali Ridho Ali Ridlo Alin Setiyorini Ambariyanto Ambariyanto Amelia C. Anggelina Amelia Cahya Anggelina Angelina Ferawaty Siregar Angelina Ferawaty Siregar Angelina, Amelia Cahya Anggi Setiabudi Aninditia Sabdaningsih Aninditia Sabdaningsih Anita Dwi Kartika Annisa Rhamadany Anuwat Nateewathana Arief Dwi Kurniawan Avigail, Yolanda Bunjamin Dharma Chrisna Adhi Suryono Dafit Ariyanto Dewi, Lutfianna Fatma Diah Ayu Mustofa Diah Tri OktaviyantI Dinda Richa Kumarahaqi Dinny Anjang Sari Dony Bayu Putra Pamungkas Dwicahyo Setiyo Wibowo Edi Setiyono Endah Sari Endang Sri Susilo Endang Sri Susilo Ervia Yudiati Ervia Yudiati Esti Rudiana Eunike Noviana Pranoto Evi Lutfiyani Fadhliyah Idris Gunawan Widi Santosa Gunawan Widi Santosa Hadi Endrawati Haeruddin Haeruddin Haeruddin Haeruddin Hendrayana Hendrayana Hidayati, Jelita Rahma Hilda Ayu Aprilia Hilda Ayu Aprilia Idris, Riyada Iin Putriyani Irwani Irwani Ita Riniatsih Kartika Dewi Koichiro Awai Lutfianna Fatma Dewi Ma'ruf, Widodo Farid Masnah Jumiati Mijil Ciptaning Dananjoyo Monika, Rika Muhammad Fatoni Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Muhammad Zainuddin Mujiyanto Mujiyanto Mukti K. Diana Putri Mukti K. Diana Putri Mustofa, Diah Ayu Nerva Sembiring Ni'mah, Ulin Nur Taufiq Nur Taufiq S.P.J. Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Nurul Fitrah Hasanah Nurul Fitrah Hasanah Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Person Pesona Renta Putri Hutari Girsang Retno Hartati Rhamadany, Annisa Ria Azizah Ria Azizah T.N. Ria Azizah Tri Nuraeni Ria Azizah Tri Nuraini Richardus Mahatmada Indrajati Rignolda Djamaludin Rika Monika Rini Pramesti Rini Widiyadmi Rini Widyadmi Rudhi Pribadi Ryandha Idris Sakti Imam Muchlissin Sari Budi Moria Siti Rudiyanti Sri Lintang Artono Sri Redjeki Sri Redjeki Sri Sejati Sri Yulina Wulandari Subagiyo Subagiyo Tatas H.P. Brotosudarmo Tedi Septiadi Tri Adi Nugroho Ulfah Rahmayani Ulin Ni'mah Vicencius Hendra Adhari Vincent Theodorus Siringoringo Vivi Endar Herawati Widianingsih Widianingsih Wilis Ari Setyati Wilis Ari Setyati Yolanda Avigail Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur

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