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All Journal Jurnal Kesehatan Masyarakat Biopendix: Jurnal Biologi, Pendidikan dan Terapan BIOEDUPAT: Pattimura Journal of Biology and Learning
Eifan Boyke Pattiasina
UNIVERSITAS PATTIMURA
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health

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Decreasing SGPT level and macrophage activity through CD68 expression in the Balb/c mice (Mus musculus) liver infected with Salmonella typhi after treating with atung seeds (Parinarium glaberimmun Hassk) Eifan Boyke Pattiasina; Pieter Kakisina; Ferymon Mahulette
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 1 No 1 (2021): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.827 KB) | DOI: 10.30598/bioedupat.v1.i1.pp17-23

Abstract

Normally macrophages are always in the body and spread in various body tissues such as liver tissue (Kupffer cells). Macrophages in tissue can be identified by the expression of several markers, in humans the marker is CD68. The increase and decrease in macrophage activity in the liver can also be indicated by an increase in SGPT levels so that atung seeds have the ability to inhibit the growth of S. tyhpi bacteria which contain tannin compounds which can damage microbial cell walls and form bonds with microbial functional proteins so that bacterial growth is inhibited. The purpose of this study was to determine the SGPT levels and macrophage activity. The method used is laboratory experiment. The results showed an increase in SGPT levels in the positive control (87.00 ± 2,915) and a concentration of 25% (84.20 ± 3,962) and a decrease in SGPT levels in the negative control (50.80 ± 2.168 *), 50% concentration (78.20 ± 3.114 *) and concentrations. 75% (58.20 ± 3,834), decreased macrophage activity in the liver also occurred at a concentration of 50% and at a concentration of 75%, the liver was normal, which was indicated by the resulting brown expression
ALIGNMENT DAN ANALISIS KEKERABATAN RAYAP COPTOTERMES FORMOSANUS (Isoptera: Rhinotermitidae) PADA HABITAT YANG BERBEDA DENGAN SOFTWARE BIOEDIT DAN MEGA 6 (STUDI PUSTAKA DNA SEKUENSING DARI NCBI) Muhammad Tarmizi Kubangun; Ali Awan; Fredy Leiwakabessy; Eifan Boyke Pattiasina
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 1 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue1page01-10

Abstract

Background: Bioinformatics is a science that can relate information which includes molecular biology, biochemical structure, enzymatic, cell biology, physiology and pathology by using a computerized system based on data that has been collected. Methods: sequence alignment and phylogenetic tree construction of DNA sequences registered at NCBI in the subterranean termite Coptotermes formosanus Shiraki that lives in different habitats, namely in Mississippi, Southeast Asia, and China using Bioedit and MEGA software. Results: Sequence alignment showed that termite C. formosanus isolated from MS12 gene COII (FJ870577.1) in the Mississippi area had a base pair of 790 bp, termite C. formosanus isolated from Dinghu Mountain gene COII (KU257993.1) in China has a base pair of 779 bp, and the termite C. formosanus isolated from CF003JP gene COII (EF379943.1) in Southeast Asia has a base pair of 676 bp. The construction of the phylogenetic tree shows that the termite C. formosanus isolated from Dinghu Mountain gene COII (KU257993.1) in the China region has a close kinship with the termite C. formosanus isolated from CF003JP gene COII (EF379943.1) in the Southeast Asia region, while The termite C. formosanus isolated from MS12 gene COII (FJ870577.1) in the Mississippi area is distantly related to C. formosanus (KU257993.1) from China and C. formosanus (EF379943.1) from Southeast Asia. Conclusion: Mississippi has a base pair of 790 bp, termite C. formosanus isolated from Dinghu Mountain gene COII (KU257993.1), China has a base pair of 779 bp, and termite C. formosanus isolated from CF003JP gene COII (EF379943.1), Southeast Asia has a base pair of 676 bp. China is closely related to the termite C. formosanus isolated from COII gene CF003JP (EF379943.1), Southeast Asia, while the termite C. formosanus isolated from MS12 gene COII (FJ870577.1), Mississippi is distantly related to C. formosanus (KU257993.1) from China and C. formosanus (EF379943.1) from Southeast Asia.
PROFIL STRUKTUR KOMUNITAS LAMUN DI PERAIRAN PANTAI KELAPA INDAH DESA MAHU KECAMATAN SAPARUA TIMUR SEBAGAI SUMBER PEMBELAJARAN BIOLOGI Prelly Tuapattinaya; Eifan Boyke Pattiasina; Johana Pattipeilohy; Marcelino Sahetapy; Chilf Wattimena
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 10 No 1 (2023): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol10issue1page148-155

Abstract

Background: Seagrass is a higher plant (Anthophyta) that lives and grows immersed in the marine environment; have vessels, have rhizomes, have roots, and reproduce generatively (seeds) and vegetatively. Seagrass is one of the natural resource ecosystem plants that lives in shallow waters and has many benefits for biota associated with the surrounding environment. Several studies related to seagrass ecosystems have been carried out in Maluku waters, but there are no records regarding the structural profile of seagrass communities on Saparua Island. Methods: This research is descriptive research. Descriptive research is used to reveal information about hydrological conditions, species composition, species diversity. This research was conducted in the waters of Kelapa Indah Beach, Mahu Village, Saparua District Results: The conditions of environmental physical and chemical factors with a temperature of 280C, salinity 31 ‰, pH 7.3, and Dissolved Oxygen 6 mg/L in the waters of Mahu village are sufficient to support the growth and spread of seagrass. Composition of the types of seagrass found in the coastal waters of Mahu village are 5 types and Conclusion: The level of seagrass diversity in Mahu village waters is classified as moderate.
The Potential of Chinese Ketepeng (Cassia alata L) Cook Water On Streptozotocin Induced Blood Sugar Decrease of Mice (Mus Musculus) Balb/C Eifan Boyke Pattiasina
Jurnal Kesehatan Masyarakat Vol 19, No 2 (2023)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v19i2.42825

Abstract

Blood sugar is a metabolic disorder disease that occurs due to insufficiency of insulin hormone production or due to insulin insensitivity resulting in typical clinical manifestations in the form of increased glucose levels in the blood (hyperglycemia). This study aims to determine the effect of Chinese ketepeng leaf decoction on blood glucose levels in hyperglycemic mice. This research was carried out in the Zoology laboratory, Faculty of Mathematics and Natural Sciences, Pattimura University, Ambon. This research is an experimental laboratory with a pre and posttest research design with a control group design. This study wanted to prove whether there was an effect of giving Chinese ketepeng leaf decoction to decreasing blood sugar levels in male Balb/C mice using Chinese ketepeng leaf decoction at a dose of 3.64 mg/kg, 7.28 mg/kg, 10.92 mg/kg. The results showed that the administration of Chinese ketepeng leaves (Cassia alata L) can reduce blood sugar levels in mice (Mus musculus). The most effective dose to lower blood sugar levels is a dose of 10.92 mg/kg BB.
Co-Authors Ali Awan Chilf Wattimena Ferymon Mahulette Fredy Leiwakabessy Johana Pattipeilohy Marcelino Sahetapy Muhammad Tarmizi Kubangun Pieter Kakisina Prelly Tuapattinaya