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Sumarnie Hasto Priyono
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Environmental Science

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KONSERVASI PIPER MINIATUM Bl. DENGAN PERBANYAKAN CARA PERUNDUKAN;EKSTRAKSI DAN UJI AKTIFITAS ANTIRADIKAL BEBAS DPPH, DAN ANTIDIABETIK Priyono, Sumarnie Hasto
Jurnal Teknologi Lingkungan Vol. 9 No. 3 (2008): JURNAL TEKNOLOGI LINGKUNGAN
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.765 KB) | DOI: 10.29122/jtl.v9i3.470

Abstract

The aim of this study was to determine the potency of Piper miniatum as medicinal plant and its propagation method. Extraction and bioassays test (antioxidant activity by DPPH method and antidiabetes by á- glucocidase test) were done. Propagationwas done by bending the internodes at different maturity (R1, R2, R3) and growth medium (compost artificial, mixed husk, forest compost and soil as control treatment).The experimental design in this study was Complete Block Design, with 10 replication. The result showed that forest compost had positive respons to numberof shoot, number of new internodes, and number of leaves on R1, R2 and R3. Old internode showed positive effect at mixed husk growth medium to number of shoots (19), emergence of new internodes (21). New seedling/plantlet were obtained 4 weeksearlier at bending treatment than that of stem cutting. Bioassays test showed that methanol extract of P. miniatum had no- free radical scavenger activity but very active on á-glukocidase inhibition with LC50 for leaves and mixed of leaves and stem were 0.82 and10.75 respectively.
KAJIAN KONSERVASI BUAH MERAH MELALUI KULTUR JARINGAN TANAMAN; EKSTRASI , FRAKSINASI BUAH, UJI ANTIOKSIDAN, DAN UJI ANTIDIABETIK Priyono, Sumarnie Hasto
Jurnal Teknologi Lingkungan Vol. 9 No. 3 (2008): JURNAL TEKNOLOGI LINGKUNGAN
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.598 KB) | DOI: 10.29122/jtl.v9i3.466

Abstract

Buah merah (Pandanus conoideus Lam.) Pandanaceae has several local name in Kelila, Wamena – Papua based on its size, fruit colo, leaf color and taste. It has wide distribution and can be found low land to high land. Buah merah contains valuable nutrients and bioactive compounds in abundant/high concentration, such as: â-caroten, tocopherol, fatty acids (oleic acid, linoleic acid, linolenic acid and decanoic acid). Its cultivation had been done ocal people but not extensively. The easiest way of buah merah propagation was by separating buah merah seedling and stem cutting or byplanting its.The aim of the study were to compile the data that support plant identification of buah merah from Kelila, Wamena-Papua, to study is potency for antioxidant and antidiabetic properties and determine the best media in tissue culture for producing buah merah planlet. The result showed that shoot bud from stem explant cultured in media combination of MS + IBA (2 mg/l) + BA(5 mg/l) produce 10 plantlets, while callus came from leaf tip explant cultured in media combination of MS + BA (5mg/l)+TDZ (0.02 mg/l) The antioxidant test showed that ethyl acetate fraction had good antioxidant activity LC 50 (0.253) while petroleum ether fraction showed good antidiabetic properties with 0.5 % (0.560), 0.25 % (0.593) and control is 0.633.
PERBANYAKAN VEGETATIF SECARA CANGKOK PIPER MINIATUM BL. Priyono, Sumarnie Hasto
Jurnal Teknologi Lingkungan Vol. 11 No. 1 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (846.561 KB) | DOI: 10.29122/jtl.v11i1.1222

Abstract

Ninety percent from approximately 1200 plant species belonged to Piper genus(Piperaceae) have not been explorated their phytochemical potency and biological activities. Piper miniatum has beeing used traditionally in Papua as spices and tonic. However, the propagation effort has never been done yet so its population in nature is decresed gradually. Therefore, simple propagation is conducted that can be adopted by the local people in order to cultivate as well as to explore its potency as antibacterial agent. The propagation experiment was carried out using air layering method with variable treatment of internode maturity ( R1, R2, R3 ), growth medium (Selaginela sp.,Asplenium nidus root, compost) with completely randomized design with 10 replications.Air layering media using Asplenium nidus root of 18 weeks gave the best respon.Key words: Piper miniatum, Air layering.
KAJIAN KONSERVASI BUAH MERAH MELALUI KULTUR JARINGAN TANAMAN; EKSTRASI , FRAKSINASI BUAH, UJI ANTIOKSIDAN, DAN UJI ANTIDIABETIK Sumarnie Hasto Priyono
Jurnal Teknologi Lingkungan Vol. 9 No. 3 (2008): JURNAL TEKNOLOGI LINGKUNGAN
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.598 KB) | DOI: 10.29122/jtl.v9i3.466

Abstract

Buah merah (Pandanus conoideus Lam.) Pandanaceae has several local name in Kelila, Wamena – Papua based on its size, fruit colo, leaf color and taste. It has wide distribution and can be found low land to high land. Buah merah contains valuable nutrients and bioactive compounds in abundant/high concentration, such as: â-caroten, tocopherol, fatty acids (oleic acid, linoleic acid, linolenic acid and decanoic acid). Its cultivation had been done ocal people but not extensively. The easiest way of buah merah propagation was by separating buah merah seedling and stem cutting or byplanting its.The aim of the study were to compile the data that support plant identification of buah merah from Kelila, Wamena-Papua, to study is potency for antioxidant and antidiabetic properties and determine the best media in tissue culture for producing buah merah planlet. The result showed that shoot bud from stem explant cultured in media combination of MS + IBA (2 mg/l) + BA(5 mg/l) produce 10 plantlets, while callus came from leaf tip explant cultured in media combination of MS + BA (5mg/l)+TDZ (0.02 mg/l) The antioxidant test showed that ethyl acetate fraction had good antioxidant activity LC 50 (0.253) while petroleum ether fraction showed good antidiabetic properties with 0.5 % (0.560), 0.25 % (0.593) and control is 0.633.
KONSERVASI PIPER MINIATUM Bl. DENGAN PERBANYAKAN CARA PERUNDUKAN;EKSTRAKSI DAN UJI AKTIFITAS ANTIRADIKAL BEBAS DPPH, DAN ANTIDIABETIK Sumarnie Hasto Priyono
Jurnal Teknologi Lingkungan Vol. 9 No. 3 (2008): JURNAL TEKNOLOGI LINGKUNGAN
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.765 KB) | DOI: 10.29122/jtl.v9i3.470

Abstract

The aim of this study was to determine the potency of Piper miniatum as medicinal plant and its propagation method. Extraction and bioassays test (antioxidant activity by DPPH method and antidiabetes by á- glucocidase test) were done. Propagationwas done by bending the internodes at different maturity (R1, R2, R3) and growth medium (compost artificial, mixed husk, forest compost and soil as control treatment).The experimental design in this study was Complete Block Design, with 10 replication. The result showed that forest compost had positive respons to numberof shoot, number of new internodes, and number of leaves on R1, R2 and R3. Old internode showed positive effect at mixed husk growth medium to number of shoots (19), emergence of new internodes (21). New seedling/plantlet were obtained 4 weeksearlier at bending treatment than that of stem cutting. Bioassays test showed that methanol extract of P. miniatum had no- free radical scavenger activity but very active on á-glukocidase inhibition with LC50 for leaves and mixed of leaves and stem were 0.82 and10.75 respectively.
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