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All Journal KAPAL Jurnal Ilmu Pengetahuan dan Teknologi Kelautan Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik agriTECH BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi NICHE Journal of Tropical Biology

Sri Pujiyanto

Departemen Biologi, Fakultas Sains Dan Matematika, Universitas Diponegoro

Author-ID : 348503

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Mathematics

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Pemurnian Parsial dan Karakterisasi Amilase dari Bakteri Laut Arthrobacter arilaitensis LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and itâs purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50Â°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization

KARAKTERISTIK DAN SIFAT KINETIKA ENZIM KITINASE ASAL JAMUR ENTOMOPATOGEN Beauveria bassiana Elawati, Nunung Eni; Pujiyanto, Sri; Kusdiyantini, Endang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Characteristics and Kinetics of Chitinase Enzyme from Entomopathogenic Fungus Beauveria bassianaBeauveria bassiana is one of the entomopathogenic fungi that produces chitinase when infecting the host. Chitinase is widely used as biocontrol agents because it can degrade chitin into an environmentally friendly product. This study aims to characterize and test the kinetics of chitinase from B. bassiana. This characterization includes determination of pH and optimum temperature, enzyme stability and enzyme kinetics test by determining Km and Vmax value with Lineweaver-Burk equations. The result of experiment showed that the chitinase B. bassiana had pH and optimum temperature of 5 and 40ÂºC respectively. This enzyme was stable until 90 minutes incubation at 40ÂºC. The Km and Vmax values were 0.181 mg/L and 0.022 mg/L.sec respectively. The Km value is higher than Vmax, which means the affinity of the enzyme to the lower substrate requiring high substrate concentration to increase the reaction rate. It can be concluded that the chitinase activity of B. bassiana is still low.Keywords: Beauveria bassiana, characteristics and kinetics, chitinase enzyme, entomopathogenic, Lineweaver-BurkABSTRAKBeauveria bassiana merupakan salah satu jamur entomopatogen yang memproduksi kitinase saat menginfeksi inangnya. Enzim kitinase saat ini banyak digunakan sebagai agen biokontrol karena dapat mendegradasi kitin menjadi produk yang ramah lingkungan. Penelitian ini bertujuan untuk mengkarakterisasi dan menguji kinetika enzim kitinase asal jamur B. bassiana. Metode yang digunakan dalam karakterisasi ini mencakup penentuan pH dan suhu optimum, kestabilan enzim pada suhu optimumnya, dan uji kinetika enzim yang mencakup penentuan nilai Km dan Vmaks dengan persamaan Lineweaver-Burk. Hasil penelitian karakterisasi menunjukkan bahwa enzim kitinase B. bassiana mempunyai pH dan suhu optimum masing-masing 5 dan 40ÂºC. Enzim ini stabil sampai pada 90 menit inkubasi pada suhu 40ÂºC. Nilai Km diperoleh 0,181 mg/L dan Vmaks sebesar 0,022 mg/L.detik. Nilai Km lebih tinggi daripada Vmaks, yang artinya afinitas enzim terhadap substrat rendah sehingga membutuhkan konsentrasi substrat yang tinggi untuk meningkatkan kecepatan reaksi, maka dapat disimpulkan bahwa aktivitas kitinase dari B. bassiana masih tergolong rendah.Kata kunci: Beauveria bassiana, entomopatogen, enzim kitinase, karakteristik dan kinetik, Lineweaver-Burk

Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa) as Antibacterial Agent Moi, Maria Yasinta; Kusdiyantini, Endang; Pujiyanto, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.

PEMBERDAYAAN MASYARAKAT PESISIR DAN PANTAI DALAM MENINGKATKAN PRODUKTIFITAS DAN EFISIENSI DI SENTRA INDUSTRI KAPAL KAYU DI KABUPATEN BATANG Santosa, Ari Wibawa Budi; Waluyo, Bambang Sri; Pujiyanto, Sri; Astuti, Sri Rahayu Tri
Kapal: Jurnal Ilmu Pengetahuan dan Teknologi Kelautan Vol 13, No 1 (2016): Februari
Publisher : Department of Naval Architecture - Diponegoro University

ProsesÂ pembuatanÂ kapalÂ di Galangan Kapal Kayu CV Laksana Abadi BatangÂ Â Â masih menggunakan cara tradisional, saat ini mengalami kesulitan untuk memenuhiÂ jumlah pesanan yang banyak. Program KKN-PPM ini untuk membantu para pengrajin kapal kayu tradisional di BatangÂ melaluiÂ menerapkan teknologiÂ yangÂ lebih modern melalui penggunaan aplikasi software perkapalan seperti Delfship dan Maxsurf. dengan menggunakan software tersebut diharapkan pengrajin kayu tradisional mampu mempercepat proses produksi dari 120 hari/ kapal menjadi 90 hari/kapal dan juga kapal kayu yang dibuat akan lebih bagus. Dalam program KKN-PPM ini mahasiswa juga harus mengenalkan pada masyarakat pesisir di BatangÂ tentangÂ kebersihanÂ lingkungan.Â ProdusenÂ danÂ PengrajinÂ kapalÂ kayu tradisional diberikan penyuluhan K3LH, menjaga kebersihan lingkungan dari limbah buang kapal yang dapat mencemari laut sekitar. Oleh karena itu, akan diterapkan pada setiap kapal kayu yang akan dibuat teknologi tepat guna OWS (oilÂ waterÂ Separator).Â DenganÂ menerapkanÂ teknologiÂ tepatÂ gunaÂ OWS, penanaman mangroove diharapkan dapat meminimalisir pencemaran laut karena limbah buang kapal kayu.

Interaksi Kapang Patogen Fusarium oxysporum dengan Bakteri Kitinolitik Rizosfer Tanaman Jahe dan Pisang Ferniah, Rejeki Siti; Pujiyanto, Sri; Purwantisari, Susiana; Supriyadi, Supriyadi
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Fusarium oxysporum is a pathogenic fungi for many plants. The fungi have chitin cell wall that can be degraded by chitinase fromchitinolytic bacteria. Aim of this research is determine how the interaction between the bacteria and F.oxysporum. Bacteria were isolatedfrom plant rizosfere. Chitinolytic activity were measured based on the clear zone around the colony in chitin medium. Bacteria and fungiinteraction were determined by an antagonistic test. This research showed that there were 9 chitinolytic bacteria. J4 and P3 had highchitinolytic index, that are 3 and 3.33, respectively. The two isolates antagonist to F.oxysporum, which the bacteria prevent growth of thefungi. The J4 and P3 are alternative biofungicide for F.oxysporum.

Produksi Dan Ekstraksi Inhibitor Alfa Glukosidase Dari Isolat Aktinomiset Jp-3 Pujiyanto, Sri; Ferniah, Rejeki Siti; S, Sunarno
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Alpha-glucosidase inhibitors are compounds that can prevent the digestion of complex carbohydrates into glucose, so potentially used as a diabetes drug. This study aims to examine the production and extraction of alpha-glucosidase inhibitor compound from Isolate Aktinomiset JP-3 from the sea. The supernatant obtained from the culture of the JP-3 isolate was extracted using various solvents to obtain the active compound. The solvents used were chloroform, methanol, and ethyl acetate. An assay of inhibitor activity of the Î±-glucosidase using p-nitrophenyl Î±-D-glucopyranoside substrate. The activity of the enzyme is measured based on the absorbance of p-nitrophenol produced from the breaking reaction of the substrate. The results showed that extraction of alpha-glucosidase inhibitor compound with ethyl acetate yielded extract with highest inhibitor activity.Â Keywords: alpha-glucosidase inhibitors, actinomycetes, diabetes, extraction, fractionation

Isolasi Dan Uji Aktivitas Kitinase Isolat Bakteri Dari Kawasan Geotermal Dieng Nafisah, Hidayatun; Pujiyanto, Sri; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Chitinase (EC.3.2.2.14) is an enzyme which can degradatechitin became N-acetilglucosamin. Chitinase has many benefits made the demand of it increases. High demands spur its availability in large quantities, cheap, fast production, resistant to any physical factor and chemical environment. Rapid and resistant enzyme production to environment factor can be obtained using chitinolitic bacteria of Geothermal Dieng. The utilization of chitin as bacterial growth substrate from waste of shell crab can be done considering high prices of commercial chitin on the market. The purpose of the research is to get the isolate of termoleranchitinolitic of watery mud in Geothermal Dieng and to know the character of the chosen isolate producing highest chinitase activity type of chitin source treatment and pH of media production. The research is done by growing the chitinolitic in the room temperature for 14 days. The experimental design used in this study is a complete randomized design of factorial pattern (two factors). The first factor is the type of chitin source that includes commercial chitin and chitin crab kits. The second factor is the pH of liquid chitin media for the production of enzymes, ie pH 6, 7 and 8.Chitinase activity is tested by measuring the result of sugar reduction. Obtained data is analyzed with Analysis of Variance (ANOVA). Result of isolation and selection is obtained one potential isolate, KSR 121. The isolate produce 1,4 cm of chitinolitic index after 96 hour incubation. Result of statistical test show both citin source type, pH of media production treatment and interaction were not significantly different (PËƒ0,05). KSR 121 isolate experience the highest growth of crab chitin treatment pH 8 (K2P3) with 6 hour incubation, whereas highest kinitase activity happen on crab chitin treatment pH 7 (K2P2) with 24 incubation, in amount of 0,125 (U/mL).Â Key words: N-acetil glucosamin, chtinase activity, chitinase, chitin, chitinolitic bacteria, isolation

Produksi Protease Alkalis Termostabil Dari Aspergillus flavus DUCC- K225 Dengan Ammonium Sulfat Sebagai Sumber Nitrogen Putra, Mohammad Affan Dwica; Rukmi, MG Isworo; Pujiyanto, Sri; Mulyani, Nies S
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

ProteaseÂ is a protein hydrolytic enzyme whichÂ can be generated by a variety of microorganisms, includingÂ mold. Aspergillus flavus K225, DUCC is indigenous mold isolated from lime soil of Madura which isÂ have potential as a alkaline proteaseÂ producer. This research aims was to know the effectÂ of ammonium sulfate as nitrogen source for the production of protease enzymes by Aspergillus flavus DUCC-K225. The production of alkalineÂ protease were conducted inÂ submerged culture medium with agitation. Fermentation medium used was modification Czapeks Dox Broth containing 2% casein. Incubation is carried out for 7 days.Â The results showed that ammonium sulfate is a good source of nitrogen for growth and production of aklaine protease enzyme, which is demonstrated byÂ higher dry weight, the protease activity, the protein content and the specific activities, comparing thoseÂ on standard medium using sodium nitrate as N source.Â

Aktivitas Inhibitor Î±-Amilase Ekstrak Etanol Tanaman Brotowali (Tinospora crispa L.) Pujiyanto, Sri; Wijanarka, W; Raharjo, Budi; Anggraeni, Via
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Brotowali plant (Tinospora crispa L.) is a traditional Indonesian medicinal plant which has many benefits including for diabetes drugs. Diabetes mellitus (DM) is a metabolic abnormality caused by an increase in blood sugar levels. The purpose of this study was to determine the activity of Î±-amylase inhibitors of Brotowali (T. crispa L.) ethanol extract of plants. Extraction is done by maceration followed by evaporation. The extract obtained was tested for Î±-amylase inhibitor activity. The Î±-amylase inhibition test is based on the breakdown of starch substrates into maltose and glucose which is then determined by spectrophotometer after administration of DNS. Tests are carried out on controls and samples. As a substrate is 0.5% starch solution in 100 ml of sterile aquades. The reaction mixture was incubated 25 Â° C for 10 minutes. The reaction is stopped by adding 2 ml of 3,5-dinitrosalicylic acid (DNS). All the mixed solutions are then heated to 100 Â° C for 5 minutes and allowed to cool. The change in color of the solution is then measured for its absorbance at a wavelength of 540 nm. As a comparison used a control test that did not use extract samples. The results of Î±-amylase inhibitor activity test showed that ethanol extract with a concentration of 1000 Î¼g / mL had the highest inhibitory activity value of 95.06% compared to extract concentration of 500 Î¼g / mL, 250 Î¼g / mL, 125 Î¼g / mL and 62.5 Î¼g / mL. The results of testing the effect of substrate concentration showed that 0.5% starch concentration had the highest inhibitory value of 9.52% compared to 2%, 1% and 0.25% concentrations

Kualitas Simplisia Tanaman Biofarmaka Curcuma domestica Setelah Proses Pemanasan Pada Suhu Dan Waktu Bervariasi Kusumaningrum, Hermin Pancasakti; Kusdiyantini, Endang; Pujiyanto, Sri
Bioma : Berkala Ilmiah Biologi Vol. 17, No.1, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Curcuma domestica is one of traditional medicinal plants that is found in Gunungpati Semarang. However the dried product do not achieve optimal standard quality for simpliciaÂ in terms of microbial contaminant and in an industrial scale household. Knowledge on how to useÂ sterilizationÂ Â to produce better simplicia and reducing contaminant has not been known by farmers, yet. The purpose of this activity was to obtain the best heating treatment on sterilization of Curcuma simplicia using several temperature under sunlight and oven device. It was also want toÂ show microbial growth after heating at several times and theirÂ influence on the quality of simplicia after treatment. The method was conducted byÂ simplicia sterilization of C. domestica using sunlight sterilization for a week and using oven at a temperature of 300C, 400C, 500C and 600C for 1, 3, 6, 9, 12, 24, and 48 hours. The results showed that heating at temperature of 500C for 48 hours obtained the best simplicia, followed by heating at a temperature of 600C for 16-48 hours without contaminants after storing period for 3 months. Â Key words :Curcuma, sterilization, heat, microbia

Co-Authors Achirul Nditasari Agung Suprihadi Agung Suprihadi Ahmad Qi Sahlan Anang S. Achmadi Anggraeni, Via Annisa Widyasari Anto Budiharjo Ari Wibawa Budi Santosa Ariyani, Mei Dwi Bambang Sri Waluyo Budi Raharjo Budi Raharjo Bunga Fajriani Debby Widiyanti Dewi, Tirta Kumala Diani Ajeng Prahesti Dinda Khairunnisa Elawati, Nunung Eni Elawati, Nunung Eni Endang Kusdiyantini Endang Kusdiyantini Fahrurrozi Fahrurrozi Fathmah, Ema Nuzula Fatin, Nuhaul Fitri Ulfana Risky Fitria Fitria Gabriela Christy Sabbathini Genoveva Preta Angelika Ghaida Afra Akhsani Hermin Pancasakti Kusumaningrum Hessy Novita Hesti Tri Rahayu Jepri Agung Priyanto, Jepri Agung Luthfian Nur Afifi M Muslikha Maerani Sumarno Mamik Setyowati Maulida Aglinia MG Isworo Rukmi Moi, Maria Yasinta Mulyani, Nies S Nafisah, Hidayatun Nanik Rahmani NANIK RAHMANI Novi Alvita Pratama Nunung Eni Elawati Nurmalisa Rizko Nurrizqi Oktavia Nurul Inayah Pramita Dian Pramitasari Putra, Mohammad Affan Dwica Putri Ramadhani R. Rahardian Rahmah Qisti Nadina Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi RATIH DEWI HASTUTI Rejeki Siti Ferniah Ridho Mathori Ikhwan Rifka A. N. Safitri Ristia Rachmatunnisa Riza Laksita Devi Mutiaratri Roseliana Fitri Roslenawati Roslenawati RS Ferniah Sarsa A. Nisa Shelina Nurunnisa Mawarni Sigit Hananto Siti Nur Jannah Sri Rahayu Tri Astuti Sugiyono Saputra Sunarno s Sunarno Sunarno Suprihadi Suprihadi Supriyadi Supriyadi Susiana Purwantisari T. A. Lestari Taufiq P. Nugraha Tia Erfianti Tsania Dyna Falasifa w wijanarka Wahyu Aji Mahardhika Wandita, Ryan Hilda Wijanarka Wijanarka Wijanarka, W Yopi Yopi YOPI YOPI Yudi Yunanto

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