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Evaluating the use of loop-mediated isothermal amplification (LAMP) method for detection of Mycobacterium tuberculosis in Indonesian clinical isolates Sudarmono, Pratiwi; Hotta, Hak; Arief, Holy; Sukarso, Triyani; Adianti, Myrna; Sudiro, Tjahjani M.; Tsuji, Hidetaka; Oshibe, Tomohiro; Lisdawati, Vivi
Medical Journal of Indonesia Vol 21, No 4 (2012): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.167 KB) | DOI: 10.13181/mji.v21i4.502

Abstract

Background: Loop-mediated isothermal amplification (LAMP) is a method already claimed as a simple technique to amplify DNA/ RNA using four to six primers as “a set” from conserved sequence of target gene. In this study we optimize the use of LAMP for detection of Mycobacterium tuberculosis in clinical isolates from Indonesia.Methods: Procedures to perform LAMP were optimized, then the method was applied to 122 archieved samples of DNA’s Mtb from clinical TB patients with Acid Fast Bacilli (AFB) smears positive. The samples were obtained in 2008 from 13 provinces in Indonesia for genotyping study, which then become collections of Center for Biomedical and Basic Technology of Health (CBBTH), NIHRD Indonesia. The optimization tests include sensitivity and specificity tests of several sets primers, which were evaluated using 10-fold serially diluted DNA of Mtb H37Rv and 12 species of Mycobacteria. Three equipments consisted of LAMP turbidimeter, heating block and water bath were compared for its ability in DNA amplification. Detection of M. tuberculosis from clinical isolates used set primers specific for gyrB gene, amplicon was detected with UV fluorescence system.Results: The results showed that the highest sensitivity was obtained using the set primers specific for 16S rRNA and gyrB which could detect 10.0 fg to 1.0 pg genomic DNA of Mtb H37Rv. The set primers specific for gyrB gene was the most specific primers. Application of LAMP using gyrB set primers on Indonesian clinical isolates showed 94.2% (114/121) positivity rate.Conclusion: LAMP method is potentially used in TB diagnosis in Indonesia. (Med J Indones. 2012;21:188-95)Keywords: Loop-mediated isothermal amplification, rim gene, 16S rRNA gene, gyrB gene, Mycobacterium tuberculosis
COMBINATION THERAPY OF MASSAGE AND TEMU IRENG HERBAL (Curcuma aeoruginosa Roxb.) TO INCREASE CHILD APPETITES AND FOOD INTAKE Myrna Adianti; Resti Ella Pramesti; Edith Frederika Puruhito
Journal of Vocational Health Studies Vol. 4 No. 1 (2020): July 2020 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V4.I1.2020.1-4

Abstract

Background: Appetite is a condition where a person has an urge to satisfy activities in eating. Lack of appetite in children is characterized by eating small portions, reluctant to refuse when it comes time to eat, take a long time to finish a meal, playing with the food, etc. Children are at the stage of high curiosity and very active, which mean the energy needed must be sufficient. If the energy and nutrition needs are not sufficient it might lead to a slowdown in physical and psychological growth. Purpose: The purpose of this case study is to observe whether Swedish massage and children’s massage with a combination of Temu Ireng herba (Curcuma aeruginosa Roxb.) could increase appetite in school-age children. Results: The results of massage therapy with Temu Ireng decotion can increase appetite in children based on the increasing number of calories consumed before therapy from 1,267 kcal to 2,050 kcal per day after therapy session, and an increase in body weight of 0.9 Kg after 8 weeks of therapy. Conclusion: Providing Swedish massage therapy with a combination of Temu Ireng herba (Curcuma aeruginosa Roxb.) could increase appetite in schoolage children with the result of increasing the number of daily calories intake and body weight. 
TRAINING AND ASSISTANCE AN NISAA’ QURAN RECITATION GROUP RUNGKUT IN UTILIZING TANAMAN OBAT KELUARGA (TOGA) INTO HERBAL PRODUCTS Rini Hamsidi; Dwi Setiani Sumardiko; Ilkafah Ilkafah; Edith Frederika Puruhito; Ratna Wahyuni; Maya Septriana; Myrna Adianti; Ario Imandiri; Taufiqurrahman Sidqi
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 2 No. 2 (2020): DECEMBER 2020
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.363 KB) | DOI: 10.20473/dc.V2.I2.2020.76-79

Abstract

Background: The corona virus pandemic (COVID-19) is spreading rapidly in a number of countries, including Indonesia. In order to deal with the increasingly widespread COVID-19, the government has established health and safety protocols by staying at home and maintaining distance, washing hands frequently with soap with running water or using hand sanitizers and increasing the body's immune system. One way to increase the immune system is through the use of Family Medicinal Plants (TOGA). Purpose: Transfer of knowledge and technology to An Nisaa 'study group partners through counseling on the use of TOGA by using appropriate technology to produce natural Hand Sanitizer products and instant herbal empon-empon in powder form which makes it easier to use daily. Methods: Methods of extension (lectures and discussions), training and mentoring using the Good Traditional Medicine Manufacturing Method (CPOTB) in producing natural Hand Sanitizer products and instant herbal medicine. Results: The expected result of this program is the use of TOGA by the community, especially partners to produce immune system-enhancing herbal products that are safe and can be stored for a long time to maintain health, especially families during the COVID 19 pandemic. Conclusion: This program increased knowledge and the skills of the An Nisaa 'Rungkut recitation group seen from the enthusiasm and ability of the participants in following and practicing the making of herbal medicine and hand sanitizers.
Low Back Pain Therapy with Back Massage, Acupressure and Ginger Compress Aura, Jihan; Purwitasari, Neny; Adianti, Myrna
Nusantara Science and Technology Proceedings The 3rd International Conference on Vocational Innovation and Applied Sciences (ICVIAS) 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Low back pain (LBP) is defined as a sensation of pain and discomfort localized below the 12th rib and above inferior gluteal folds. In this case study, based on traditional medicine the client's LBP is due to kidney deficiency syndrome especially the kidney Qi and the damp-cold pathogen attack. Which is caused by bad habits, trauma, the living environment tends to be humid that can injure the kidneys, bladder which makes the kidney Qi weak and cause damp-cold pathogens to settle easily. The purpose is to know the effect of combination therapy for a back massage, acupressure at Shenshu (BL23), Weizhong (BL 40), Taixi (KI 3), and Sanyinjiao (SP 6) and ginger (Zingiber officinale Rosc.) compress on low back pain sufferers. For the methods, the clients with LBP were given massage therapy in the back and acupressure points at Shenshu (BL 23), Weizhong (BL 40), Taixi (KI 3), and Sanyinjiao (SP 6) as well as given ginger herbal compresses (Zingiber officinale Rosc.) 12 times within 1 week 2 times of therapy. The ginger used is red ginger as much as 20 grams each time therapy. The results of the treatment of LBP were that massage combination therapy on the back area and acupressure at the points Shenshu (BL 23), Weizhong (BL 40), Taixi (KI 3), and Sanyinjiao (SP 6) and presented a ginger herbal compress (Zingiber officinale Rosc.) can help with low back pain. Conclusion: Back Massage therapy, acupressure, and ginger compress could reduce low back pain.
ANALYSIS OF THE INCIDENT RATE OF PEOPLE WITH A POTENTIAL RISK FOR HYPERTENTION AND DIABETES IN KENJERAN SUB-DISTRICT, SURABAYA Maya Septriana; Aliyah Siti Sundari; Diyantoro Diyantoro; Dwi Wahyu Indriati; Anita Kurniati; Ni Nyoman Purwani; Tofan Agung Eka Prasetya; Fadilatus Sukma Ika Noviarni; Shelly Wulandari; Myrna Adianti
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 1 No. 1 (2019): June 2019
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.389 KB) | DOI: 10.20473/dc.V1.I1.2019.11-15

Abstract

Background: The number of people with diabetes mellitus in Indonesia is increasing every year. The data in 2016 showed that 1 of 11 adults could have diabetes mellitus. Uncontrolled and untreated diabetes mellitus can cause complications. Objectives: This activity aimed to determine the group of age that have a potential risk of hypertension and diabetes so that it could minimize the prevalence of disease and optimize human productivity. Methods: (1) Counseling about hypertension and diabetes, (2) Screening the people with a potential risk of hypertension using tensimeter and diabetes prediction using point-of-care testing (POCT) and urine dipstick. Results: The data were collected from 75 respondents with characteristics about 85,33% female and 14,67% male. About 25 respondents (33,33%) were suspected of hypertension. Based on age group, it showed that the highest incident rate of hypertension was found in the age group of 41-60 years (41,86%), followed by the age group of 61-80 years (20%), 21-40 years (13,33%), and age under 20 years and above 80 years (0%). The majority of respondents had a normal glucose level(90,67%). Based on gender, there was 1 male respondent (9,09%)and5female respondents  (7,81%) having a glucose level higher than normal value (hyperglycemia). Conclusion:  The age group with the highest potential risk for hypertension was 41-60 years, while for diabetes was 61-80 years. This activity provided information to prevent the potential risk leading to hypertension and diabetes.
INCREASING PUBLIC PARTICIPATION TO PREVENT THE SPREAD OF COVID-19 IN DUKUH KUPANG SURABAYA Winarno; Deny Arifianto; Myrna Adianti; Eva Inaiyah Agustin; Sisca Dina Nur Nahdliyah; Elsyea Adia Tunggadewi; Ali Suryaperdana Agoes
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 6 No. 1 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i1.2022.235-243

Abstract

Novel Coronavirus 2019 (covid-19) is a generation of the coronavirus that humans have never identified. This virus was first reported to cause an outbreak in Wuhan, China, in December 2019. Until now, covid-19 infection cases have been reported in many countries, such as Thailand, Japan, South Korea, Iran, Italy, Germany, and more than 90 countries worldwide. Until November 1, 2020, a total of 412.784 positive cases of covid-19 were recorded, with 341.942 recovered patients and 13.943 deaths in Indonesia. The purpose of this event is to anticipate the spread of Covid-19 by reminding the public to maintain health protocols and other alternatives in preserving health, also increasing body immunity through massage and traditional Indonesian herbal medicine. This event received a good response from the public. It was evidenced by the number of participants exceeding the predetermined quota and enthusiastic in following the speaker's material. The event was a success and was well received by the participants as well as the local government. After following this event, participants can practise the knowledge obtained from the presenters to maintain and protect their health and immunity from Covid-19. Keywords: Covid-19, Community service, body immunity DAFTAR PUSTAKA Amri, Sofan. Iif Khoiru Ahmadi. 2010. Proses Pembelajaran Kreatif dan Inovatif Dalam Kelas: Metode, Landasan Teoritis-Praktis dan Penerapannya. Jakarta: PT. Prestasi Pustakaraya. Badan Pusat Statistik Kota Surabaya. “Jumlah RT RW Kecamatan Dukuh Pakis Menurut Kelurahan Tahun 2017”. surabayakota.bps.go.id. https://surabayakota.bps.go.id/statictable/2018/04/19/594/jumlah-rt-rw-kecamatan-dukuh-pakis-menurut-kelurahan-tahun-2017.html. (diakses 15 Desember 2020). Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, Zhang L, Fan G, Xu J, Gu X, Cheng Z. 2020. “Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China”. Lancet 2020: 395: 497-506. doi: https://doi.org/10.1016/S0140-6736(20)30183-5. Kementerian Kesehatan Republik Indonesia. 2020. Pedoman Kesiapsiagaan Menghadapi Coronavirus Disease (covid-19). Jakarta: Direktorat Jenderal Pencegahan dan Pengendalian Penyakit (P2P). Komite Penanganan Covid-19 dan Pemulihan Ekonomi Nasional. “Pasien Sembuh Harian Mencapai 4.141 Orang”. covid19.go.id. https://covid19.go.id/berita/pasien-sembuh-harian-mencapai-4141-orang. (diakses 15 Desember 2020). Kumar, M., & Dwivedi, S. 2020. “Impact of Coronavirus Imposed Lockdown on Indian Population and their Habits”. International Journal of Science and Healthcare Research Vol.5 Issue: 2: April-June 2020. ISSN: 2455-7587. Li, S., Wang, Y., Xue, J., Zhao, N., & Zhu, T. 2020. “The impact of covid-19 epidemic declaration on psychological consequences: A study on active weibo users”. International Journal of Environmental Research and Public Health, 17(6), 1–9. doi: https://doi.org/10.3390/ijerph17062032. Pemerintah Kota Surabaya, “Statistik”. lawancovid-19.surabaya.go.id. https://lawancovid-19.surabaya.go.id/visualisasi/graph. (diakses 15 Desember 2020). Rizma Riyandi. “Data Kecamatan Surabaya: Dukuh Pakis, Penduduk dan Wilayah”. ayosurabaya.com. https://www.ayosurabaya.com/read/2020/09/28/3230/data-kecamatan-surabaya-dukuh-pakis-penduduk-dan-wilayah. (diakses 15 Desember 2020). Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, Zhao X, Huang B, Shi W, Lu R, Niu P. 2020. “A novel coronavirus from patients with pneumonia in China, 2019”. New England Journal of Medicine 2020: 382: 727-733. doi: 10.1056/NEJMoa2001017
HYPERTENSION THERAPY USING ACUPUNCTURE AND HERBALS OF LEDS AND CARROTS Ulfa Nur Khasanah; Ario Imandiri; Myrna Adianti
Journal of Vocational Health Studies Vol. 2 No. 2 (2018): November 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.164 KB) | DOI: 10.20473/jvhs.V2.I2.2018.67-73

Abstract

Background: Hypertension or high blood pressure is an increase in systolic blood pressure of more than 140 mmHg and diastolic blood pressure of more than 90 mmHg in two measurements with an interval of five minutes in rest or quiet state. Hypertension in Traditional Chinese Medicine (TCM) is categorized in Xuan Yuan (vertigo) and Tou Tong (headache). Purpose: To determine the effect of acupuncture therapy using Fengchi (GB20), Taichong (LR3), Hegu (LI4) points and 2 additional points, namely Xuehai (SP10) and Chize (LU5) and herbs therapy for celery (Apium graveolens L.) and carrots (Daucus carota L.) for people with hypertension. Methods: Treatment of hypertension with acupuncture and herbs therapy. Acupuncture therapy was carried out at Fengchi (GB20), Taichong (LR3), Hegu (LI4) points with the principle of liver fire sedation and Xuehai (SP10) and Chize (LU5) with the tonification principle. In herbs therapy, patients were given fresh celery (Apium graveolens L.) and carrots (Daucus carota L.) which had a hypotensive effect. Results: Hypertension treatment with acupuncture therapy was given 12 times, 3 times a week. Herbs therapy was given for 4 weeks, taken once a day in the afternoon with a dose of 200 ml. This therapy could reduce the patient's blood pressure. Conclusion: Acupuncture therapy in Fengchi (GB20), Taichong (LR3), Hegu (LI4) Xuehai (SP10), Chize (LU5) plus fresh celery herbs (Apium graveolens L.) and carrots (Daucus carota L.) could reduce blood pressure and additional complaints in hypertensive patients
THERAPY FOR IRREGULAR MENSTRUATION WITH ACUPUNTURE AND HERBAL PEGAGAN (CENTELLA ASIATICA (L.)) Evin Dwi Prayuni; Ario Imandiri; Myrna Adianti
Journal of Vocational Health Studies Vol. 2 No. 2 (2018): November 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.632 KB) | DOI: 10.20473/jvhs.V2.I2.2018.86-91

Abstract

Background : Menstruation is bleeding periodic scale and cyclical from uterus extrication (deskuamasi) endometrium. Menstruation bleeding is complex interaction result wich involve hormone system and body system, that are hypothalamus, hypofisis, ovarium and uterus. Every woman has different menstruation cycle. The causes of irregular menstruation are genetic and bad habit life. While according to TCM, irregular menstruation is Yue Jing Bu Tiao. Purpose : to knowing the effect of acupuncture therapy and point of Guanyuan (CV 4), Shenshu (BL 23), Sanyinjao (SP 6) and Shenmen (HT 7). Therapy was given 12 times, 3 times every week. Herbal therapy wich given was pegagan (Centella asiatica (L)) with dose 0,3 and consumed everyday after eat. Result : therapy that used acupuncture and herbal in irregular menstruation case showing menstruation cycle change, that was menstruation cycle became shorter from 76 days to 41 days. Conclusion : acupuncture therapy on point of Guanyuan (CV 4), Shenshu (BL 23), Sanyinjao (SP 6), Shenmen (HT 7), and pegagan herbal (Centella asiatica (L)) could shorten menstruation cycle from 76 days to 41 days.
THERAPY FOR HYPERTENSION WITH ACUPUNTURE AND HERBAL CUCUMBER (Cucumis sativus (L.)) Yuninda Asyroful Kamaliyah; Ario Imandiri; Myrna Adianti
Journal of Vocational Health Studies Vol. 4 No. 1 (2020): July 2020 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V4.I1.2020.41-49

Abstract

Background : Hypertension or high blood pressure is an increase in systolic blood presure ≥ 140 mmHg and diastolic blood pressure ≥ 90 mmHg in a person's body, when two measurements are taken with an interval of five minutes so that the body is resting or quite calm. In TCM, hypertension is categorized into two, is called Xuan Yun (vertigo) and Tou Feng (headache). Cases of hypertension experienced by patients is the patient's blood pressure has increased 188/100 mmHg because stopped taking medicine for 2 years. The patient is classified as hypertensive with differentiated liver fire hyperactivity syndrome. Purpose: To determine the effect of acupuncture combination therapy at Shenmen (HT 7), Fuliu (KI 7), Taichong (LR 3), Xingjian (LR 2), and Yinlingquan (SP 9) herbal fruit and herbal cucumber (Cucumis sativus) in diseases hypertension. Methods : Treatment of Hypertension with acupuncture and herbal therapy methods. Acupuncture therapy was carried out at Shenmen (HT 7), Fuliu (KI 7), Taichong (LR 3), points for tonification, the Point of Xingjian (LR 2) and Yinlingquan (SP 9) points for sedation. Result : The water content 0f 95% in cucumber which is a diuretic can reduce blood pressure in hypertension patient by giving cucumber herbs (Cucumis sativus) once a day, for 27 days with a dose of 150 grams of cucumber. And acupuncture theraphy 12 times, once every 2 days. Conclusion : Acupuncture and herbal therapy can be used to treat diseases in hypertension patients.
Free Radical Scavenging and Analgesic Activities of 70% Ethanol Extract of Luvunga sarmentosa (BI.) Kurz from Central Kalimantan Utami Islamiati; Hanifah Khairun Nisa; Hilkatul Ilmi; Lidya Tumewu; Myrna Adianti; Tutik Sri Wahyuni; Aty Widyawaruyanti; Achmad Fuad Hafid
Borneo Journal of Pharmacy Vol 5 No 1 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i1.2983

Abstract

Luvunga sarmentosa, commonly known as saluang belum, is widely used in Kalimantan to relieve pains, rheumatism, boost the immune system, and fever. The research on the free radical scavenging and analgesic effect of the L. sarmentosa stem extract has not been reported. This study aimed to evaluate the free radical scavenging and analgesic activity of the ethanol extract of L. sarmentosa. The L. sarmentosa stem was extracted using 70% ethanol and tested for free radical scavenging using the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and analgesic activity, acetic acid-induced writhing test, and hot plate test in an animal model. The results showed that the 70% ethanol extract of the L. sarmentosa had an anti-free radical scavenging and analgesic activity. The extract has weak free radical scavenging with an IC50 value of 293.45 µg/mL. Analgesic activity using the writhing test indicated that the extract significantly reduced the writhes count after oral administration in a dose-dependent manner compared to the negative control. Extract at a dose of 550 mg/kg BW can reduce the writhing test by 67.60% compared to others. In contrast, the diclofenac sodium reduced the number of writhes by 74.74%. While in a hot plate, the extract at a dose of 550 mg/kg BW produced a maximum possible analgesia (MPA) of 17.64%, lower than the MPA of diclofenac sodium (51.01%). Analgesic activity of the extract has higher inhibition on the writhing test than on the hot plate. The extract could be responsible for the peripheral mechanism by inhibiting the prostaglandin biosynthesis.