Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Jurnal Hortikultura Jurnal Pengkajian dan Pengembangan Teknologi Pertanian
Sudarmadi Purnomo
Balai Penelitian Tanaman Buah, Solok
Agriculture, Biological Sciences & Forestry Engineering

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Transformasi cDNA Gen 1-Aminosiklopropan-1-Asam Karboksilat Oksidase untuk Penundaan Kematangan Buah Pepaya Dampit dan Sarirona Makful, -; Purnomo, Sudarmadi; Sunyoto, -; Iswanto, R; Utami, T.I.R.
Jurnal Hortikultura Vol 14, No 2 (2004): Juni 2004
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini dilaksanakan dari bulan Januari 2002 sampai Februari 2003, bertujuan untuk mendapatkan kalustransforman gen 1-aminosiklopropan-1-asam karboksilat oksidase yang mampu hidup dan dapat berdiferensiasi,wahana untuk membuat pepaya transgenik tahan simpan, telah dilakukan di Laboratorium Biologi Molekuler, BalaiBesar Penelitian Bioteknologi dan Sumber Daya Genetik Pertanian, Bogor. Mutu buah pepaya salah satunyaditentukan oleh kesegaran buah saat dikonsumsi. Proses pemasakan buah pepaya berlangsung sangat cepat, hal inimenyulitkan dalam transportasi pepaya, terutama untuk menjangkau tempat yang jauh. Proses pemasakan buahdikontrol oleh meningkatnya konsentrasi hormon etilen yang disintesis dari 1-aminosiklopropan-1-asam karboksilat.Produksi etilen dapat ditekan dengan memblok jalur biosintesis etilen. Mekanismenya adalah membuat antisens genreg u la tor biosintesis etilen. Transformasi pepaya varietas dampit dan sarirona dilakukan menggunakan bombardemenpartikel. Rancangan percobaan adalah deskriptif kuantitatif dengan rancangan acak lengkap. Sumber eksplan pepayaberupa embrio zigotik yang digunakan untuk optimasi taraf kematian terhadap kanamisin, uji gus menggunakanplasmid pRQ6 (gen gus, NPH, promotor 35S, dan ter mi na tor NOS) dan introduksi gen interes dalam plasmid pGA643SM4 (gen antisens 1-aminosiklopropan-1-asam karboksilat oksidase, NPT II, promotor 35S, dan ter mi na tor NOS)pada me dia seleksi. Hasil penelitian menunjukkan bahwa kedua eksplan pepaya op ti mal pada kanamisin 150 mg/l, dimana pada konsentrasi ini eksplan mati seluruhnya. Pengujian gus terbanyak pada varietas sarirona 25% (25 spotbiru) jarak 9 cm, sedangkan varietas dampit 10% (9 spot biru) jarak 5 cm. Spot biru menandakan gen yang disisipitelah terintegrasi pada gen tanaman. Efisiensi gen antisens ACC oksidase pada me dia seleksi kanamisin 150 mg/lmenunjukkan 16% (14 embrio kotiledon) pada varietas dampit, sedangkan varietas sarirona tidak tumbuh.Tumbuhnya transforman pada me dia seleksi menunjukkan eksplan tersebut sudah tersisipi pGA643 SM4 yangmengandung gen tahan terhadap kanamisin (gen NPT II ).Trans for ma tion of cDNAACC ox i dize gene for de lay rip en ing on pa paya dampit and sarirona. This re search was con ducted from Jan u ary2002 to Feb ru ary 2003. The aim of this re search was to find out trans formed cal lus by aminocyclopropane carboxylicacid (ACC) ox i dize gene, there are vi a ble and be able to dif fer en ti a tion, the means to de velop pa paya trans gen ic forde lay rip en ing. The re search was con ducted at the Lab o ra tory of Mo lec u lar Bi ol ogy of Re search In sti tute for Bio tech -nol ogy and Ag ri cul tural Ge netic Re sources, Bogor. One of pa paya qual ity was de ter mined by fruit fresh ness. The pro -cess of pa paya ma tu rity was very fast. This prob lem made dif fi cult for hap pened pa paya trans por ta tion. The pro cess ofma tu rity was con trolled by in creas ing con cen tra tion of eth yl ene hor mone and it was syn the sized from1-aminocyclopropane-1-carboxylic acid. One of ef fort pressed eth yl ene pro duc tion was long dis tance block ingethylene biosynthesis path way. Mech a nism of block ing eth yl ene biosynthesis path way was made antisens of genereg u la tor. The trans for ma tion of pa paya dampit and sarirona va ri ety have been de rived by bom bard ment par ti cle. Theex per i men tal de sign was de scrip tive quan ti ta tive with ran dom ized com plete de sign. The zy gotic em bryo as explantsource were used for op ti miz ing kanamycin lev els, gus as say with pRQ6 plasmid (gus gene, NPH, 35S pro moter, andNOS ter mi na tor) and in tro duc tion in ter est gene with pGA643 SM4 plasmid (antisens ACC ox i dize gene, NPT II, 35Spro moter, NOS ter mi na tor) on the se lec tive me dium. The re sults in di cated the op ti miz ing of both pa paya on 150 mg/lkanamycin. This con cen tra tion made all explant of results. Gus as say pref er ence of sarirona 25% (25 blue spot) in dis -tance 9 cm and dampit 10% (9 blue spot) in dis tance 5 cm. Ef fi ciency of antisens ACC ox i dize gene on se lec tive me -dium con tain ing 150 mg/l kanamycin in di cated 16% (14 cotyledone em bryo) in dampit but in sarirona was not growth.Transforman growth on se lec tive me dium in di cated pGA643 SM4 have in serted to zy gotic em bryo be cause plasmidcon tain ing se lec tion gene of kanamycin (NPT II gene).
Karakterisasi dan Evaluasi Beberapa Aksesi Nanas Hadiati, Sri; Purnomo, Sudarmadi; Meldia, Y; Sukmayadi, I; -, Kartono
Jurnal Hortikultura Vol 13, No 3 (2003): SEPTEMBER 2003
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan untuk mengarakterisasi dan mengevaluasi karakter-karakter penting beberapa aksesi nanas dalam upaya mendapatkan tetua untuk perakitan varietas unggul. Penelitian dilakukan di Balai Penelitian Tanaman Buah, Solok mulai bulan  Januari 2001 sampai Februari  2002. Rancangan yang digunakan adalah acak kelompok dengan 24 nomor aksesi sebagai perlakuan dan diulang dua kali. Setiap perlakuan terdiri dari empat tanaman. Hasil penelitian menunjukkan bahwa karakter antaraksesi nanas berbeda, kecuali jumlah dan lebar daun. Aksesi dengan tepi daun tidak berduri ditampilkan oleh aksesi nomor 4C, 5C, 8C, 20C, 26C, 27C, dan 34C. Aksesi yang mempunyai karakter unggul pada komponen buah, yaitu mempunyai mahkota tunggal ditampilkan oleh semua nomor aksesi, kecuali nomor 30H dan 32H; tangkai buah pendek oleh nomor 2Q, 4C, 5C, 8C, 20C, 26C, 27C, dan 34C; bobot buah>1.000 g oleh nomor 26C, 30H, 32H, dan 34C; mata dangkal oleh nomor 2Q, 16Q, 4C, 5C, 8C, 20C, 27C, dan 34C; serta aksesi yang mempunyai diameter buah >9,5 cm ditampilkan oleh nomor 3H, 30H, 32H, 4C, 8C, 26C, 27C, dan 34C. Aksesi yang mempunyai karakter unggul pada kualitas buah, yaitu kandungan TSS ³16° Brix adalah nomor 2Q, 16Q, 18Q, 4C, 5C, 8C, 20C, 27C, dan 34C; vitamin C tinggi aksesi nomor 1MP, 45MP, 3H, 30H, 32H, 10M, 33M, dan 4C; kadar serat rendah ditampilkan oleh semua nomor aksesi kecuali nomor 1MP dan 45MP; rasio daging/hati yang besar oleh nomor 3H, 30H, 32H, 10M, dan 33M. Aksesi yang mempunyai jumlah karakter unggul terbanyak, yaitu tepi daun tidak berduri, mahkota tunggal, tangkai buah pendek, mata dangkal, diameter buah >9,5 cm, TSS ³16° Brix, kadar serat rendah ditampilkan oleh nomor 4C, 8C, 27C, dan 34C. Bobot buah ditentukan oleh karakter diameter dan panjang buah. Pada jarak taksonomi 1,95 terbagi menjadi dua kelompok, yaitu kelompok A (klon merah, hijau, queen, dan cayenne) dan kelompok B (klon merah pagar). Informasi karakter ini dapat digunakan sebagai pertimbangan untuk pemilihan tetua dalam program perakitan varietas unggul. Kata kunci: Ananas comosus; Aksesi; Karakterisasi; Evaluasi;  Pertumbuhan dan hasil; Mutu buah. ABSTRACT. The aim of the research was to characterize and evaluate the important characters of some pineapple accessions to obtain the parents for establishing the superior variety. This research was conducted at Indonesian Fruit Research Institute from January 2001 to February 2002. The experiment was arranged in a com- pletely randomized block design with 24 accessions as treatments and two replications. Each experimental unit con- sisted of four plants. The results showed that all characters were significantly different among accessions, except the number and width of leaves. The leaves margin whithout  spine were showed by accessions number of 4C, 5C, 8C, 20C, 26C, 27C, and 34C; superior characters of fruit component, i.e single crown were showed by all accessions, ex- cept accession number 30H and 32H; short peduncle showed by 2Q, 4C, 5C, 8C, 20C, 26C, 27C, and 34C; fruit weight >1000 g showed by 30H, 32H, 26C, and 34C; flat eyes showed by 2Q, 16Q, 4C, 5C, 8C, 20C, 27C, and 34C of accessions number. Superior characters of fruit quality, i.e. TSS ³16°Brix were showed by accessions number of 2Q, 16Q, 18Q, 4C, 5C, 8C, 20C, 27C, and 34; high vitamin C content showed by 1MP, 45MP, 3H, 30H, 32H, 10M, 33M, and 4C; low fibre content showed by all accessions except accessions number 1MP and 45MP; high flesh/core thickness ratio showed by 3H, 30H, 32H, 10M, and 33M. It’s looked that accessions number 4C, 8C, 27C, and 34C had more su- perior characters than the others, i..e leave margin whithout spine, single crown, short peduncle, fruit diameter >9.5 cm, TSS ³16 °Brix, and low fibre content. Fruit weight was determined by diameter and length of fruit characters. There were two clone groups at 1.95 taxonomic distance, i.e group A consisted of merah, hijau, queen, and cayenne clones, and group B consisted of merah pagar clone. The information about the characters observed could be used to obtain the parents for establishing the superior variety.
Perlakuan Biji Pepaya dalam Larutan 3-Indolebutyric Acid, Absicic Acid dan Ukuran Polibag terhadap Perkecambahan dan Pertumbuhannya Triatminingsih, Rahayu; Handayani, Sri; Subakti, Hary; Purnomo, Sudarmadi
Jurnal Hortikultura Vol 12, No 4 (2002): Desember 2002
Publisher : Indonesian Center for Horticultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hasil persilangan antartanaman berdasarkan tipe seks menghasilkan segregasi dengan proporsi yang berbeda-beda.Teknik identifikasi seks sejak fase benih sangat dibutuhkan dalam upaya mempercepat siklus generasi dan efisiensiruang uji dalam uji persilangan. Penelitian ini merupakan tahap awal untuk mengidentifikasi bibit sejak dini yaitudengan memperlakukan biji dalam larutan auksin dan ABA.Tujuan dari penelitian ini adalah untuk memperoleh datadaya pertumbuhan bibit pepaya yang diperlakukan dengan indole bu tyric acid (IBA) dan absicic acid (ABA).Penelitian ini dilaksanakan di Sumatera Barat mulai bulan Agustus 1999 sampai dengan Maret 2000. Penelitianmenggunakan Rancangan Acak Kelompok sebanyak tiga ulangan yang disusun secara faktorial. Untuk mendapatkanteknik identifikasi tersebut dapat didekati dengan mendiskripsikan benih semaian sampai dengan tanaman di polibag.Hasil penelitian menunjukkan bahwa perlakuan IBA dan ABA berpengaruh terhadap pertumbuhan daun dan tinggitanaman. IBA dan ABA tidak berpengaruh nyata terhadap di am e ter batang. Pada awal pertumbuhan, di am e ter batangdipengaruhi oleh tempat. Pengaruh IBA dan ABA nyata pada perkecambahan biji terutama untuk dampit, lokal kuningdan lokal jingga.Kata kunci : Carica pa paya; Biji pepaya; IBA; ABAAB STRACT. Triatminingsih. R., S. Handayani., H. Subakti., and S. Purnomo. 2002. The ef fect of IBA, ABAand con tainer size for seed ger mi na tion and seed grow ing of pa paya. This ex per i ment has been done at Solok Re -search In sti tute for Fruit Crops. Outpolynation the plant ac cord ing to sex type pro duced highly seg re ga tion. The pro -por tion of seg re gate was not the same. Iden tity of va ri ety is the main key in pa paya cross ing. Sex iden ti fi ca tiontech nique at seed ling was de manded. This tech nique can be used to accelarate re gen er a tion cy cle and ef fi ciency ontest ing the new clones. The aims of this ex per i ment was to find out the seed ger mi na tion and seed ling growth. The re -search was con ducted at West Sumatera from Au gust 1999 un til March 2000. The ex per i ment was us ing a Ran dom -ized Block De sign with three rep li ca tion. The tech nique can be worked out by mor phol ogy de scrip tion from seed lingup to the plant that was grown in a polybag. The re sults in di cated that ABA and IBA was sig nif i cant by af fect the leafgrowth and hight of stem. There was no sig nif i cantly dif fer ent on stem di am e ters. At early growth polybag sizes wassig nif i cantly af fect the stem di am e ter. IBA and ABA was af fected to the ger mi na tion of pa paya seed va ri ety, dampit,lo cal kuning and lo cal jingga.
PENGKAJIAN RAKITAN TEKNOLOGI BUDIDAYA KENTANG DAN STRATEGI PENGEMBANGANNYA : Studi Kasus di Wilayah Prima Tani Lahan Kering Dataran Tinggi Kabupaten Magetan Santoso, Pudji; ., Yuniarti; Purnomo, Sudarmadi; ., Subandi
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 12, No 2 (2009): Juli 2009
Publisher : Jurnal Pengkajian dan Pengembangan Teknologi Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pengkajian Rakitan Teknologi Budidaya Kentang dan Strategi Pengembangannya : Studi Kasus di Wilayah Prima Tani Lahan Kering Dataran Tinggi Kabupaten Magetan. The assessment on improving the cultivation technology of potato as demonstration plot in farmer’s land was done in Prima Tani area of Magetan regency in Genilangit village, during dry season on June – September 2007. Collecting data was done using farm record keeping method on the application of improving cultivation technology of potato which was done by cooperator farmers, while non-cooperator farmers done their potato farming using their own cultivation technology as the comparison. Improving cultivation technologies which were applied by the farmers including 1) Seed quality and seed treatment before planting, 2) The use of rational fertilization, 3) Enlargement of planting space and planting-row space, and 4) Improving pests and diseases control method. The aim of the assessment were 1) To increase productivity, yield quality and farmer’s income of potato farming using the improved cultivation technology, and 2) To find development strategy of potato farming in the assessment area. The result showed, that application of the improved cultivation technology of potato farming can increased productivity, yield quality and farmer’s income. Development strategy for potato farming in this area should be based on the principle of maximize their potency and use their opportunity in facing threats and minimize their weaknesses. Increasing the farming efficiency, productivity and yield quality should be done by application of potato farming using cooperated farming model. The use of qualified seed such as certified potato seed needed to be socialized.
Transformasi cDNA Gen 1-Aminosiklopropan-1-Asam Karboksilat Oksidase untuk Penundaan Kematangan Buah Pepaya Dampit dan Sarirona Makful, -; Purnomo, Sudarmadi; Sunyoto, -; Iswanto, R; Utami, T.I.R.
Jurnal Hortikultura Vol 14, No 2 (2004): Juni 2004
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v14n2.2004.p76-83

Abstract

Penelitian ini dilaksanakan dari bulan Januari 2002 sampai Februari 2003, bertujuan untuk mendapatkan kalustransforman gen 1-aminosiklopropan-1-asam karboksilat oksidase yang mampu hidup dan dapat berdiferensiasi,wahana untuk membuat pepaya transgenik tahan simpan, telah dilakukan di Laboratorium Biologi Molekuler, BalaiBesar Penelitian Bioteknologi dan Sumber Daya Genetik Pertanian, Bogor. Mutu buah pepaya salah satunyaditentukan oleh kesegaran buah saat dikonsumsi. Proses pemasakan buah pepaya berlangsung sangat cepat, hal inimenyulitkan dalam transportasi pepaya, terutama untuk menjangkau tempat yang jauh. Proses pemasakan buahdikontrol oleh meningkatnya konsentrasi hormon etilen yang disintesis dari 1-aminosiklopropan-1-asam karboksilat.Produksi etilen dapat ditekan dengan memblok jalur biosintesis etilen. Mekanismenya adalah membuat antisens genreg u la tor biosintesis etilen. Transformasi pepaya varietas dampit dan sarirona dilakukan menggunakan bombardemenpartikel. Rancangan percobaan adalah deskriptif kuantitatif dengan rancangan acak lengkap. Sumber eksplan pepayaberupa embrio zigotik yang digunakan untuk optimasi taraf kematian terhadap kanamisin, uji gus menggunakanplasmid pRQ6 (gen gus, NPH, promotor 35S, dan ter mi na tor NOS) dan introduksi gen interes dalam plasmid pGA643SM4 (gen antisens 1-aminosiklopropan-1-asam karboksilat oksidase, NPT II, promotor 35S, dan ter mi na tor NOS)pada me dia seleksi. Hasil penelitian menunjukkan bahwa kedua eksplan pepaya op ti mal pada kanamisin 150 mg/l, dimana pada konsentrasi ini eksplan mati seluruhnya. Pengujian gus terbanyak pada varietas sarirona 25% (25 spotbiru) jarak 9 cm, sedangkan varietas dampit 10% (9 spot biru) jarak 5 cm. Spot biru menandakan gen yang disisipitelah terintegrasi pada gen tanaman. Efisiensi gen antisens ACC oksidase pada me dia seleksi kanamisin 150 mg/lmenunjukkan 16% (14 embrio kotiledon) pada varietas dampit, sedangkan varietas sarirona tidak tumbuh.Tumbuhnya transforman pada me dia seleksi menunjukkan eksplan tersebut sudah tersisipi pGA643 SM4 yangmengandung gen tahan terhadap kanamisin (gen NPT II ).Trans for ma tion of cDNAACC ox i dize gene for de lay rip en ing on pa paya dampit and sarirona. This re search was con ducted from Jan u ary2002 to Feb ru ary 2003. The aim of this re search was to find out trans formed cal lus by aminocyclopropane carboxylicacid (ACC) ox i dize gene, there are vi a ble and be able to dif fer en ti a tion, the means to de velop pa paya trans gen ic forde lay rip en ing. The re search was con ducted at the Lab o ra tory of Mo lec u lar Bi ol ogy of Re search In sti tute for Bio tech -nol ogy and Ag ri cul tural Ge netic Re sources, Bogor. One of pa paya qual ity was de ter mined by fruit fresh ness. The pro -cess of pa paya ma tu rity was very fast. This prob lem made dif fi cult for hap pened pa paya trans por ta tion. The pro cess ofma tu rity was con trolled by in creas ing con cen tra tion of eth yl ene hor mone and it was syn the sized from1-aminocyclopropane-1-carboxylic acid. One of ef fort pressed eth yl ene pro duc tion was long dis tance block ingethylene biosynthesis path way. Mech a nism of block ing eth yl ene biosynthesis path way was made antisens of genereg u la tor. The trans for ma tion of pa paya dampit and sarirona va ri ety have been de rived by bom bard ment par ti cle. Theex per i men tal de sign was de scrip tive quan ti ta tive with ran dom ized com plete de sign. The zy gotic em bryo as explantsource were used for op ti miz ing kanamycin lev els, gus as say with pRQ6 plasmid (gus gene, NPH, 35S pro moter, andNOS ter mi na tor) and in tro duc tion in ter est gene with pGA643 SM4 plasmid (antisens ACC ox i dize gene, NPT II, 35Spro moter, NOS ter mi na tor) on the se lec tive me dium. The re sults in di cated the op ti miz ing of both pa paya on 150 mg/lkanamycin. This con cen tra tion made all explant of results. Gus as say pref er ence of sarirona 25% (25 blue spot) in dis -tance 9 cm and dampit 10% (9 blue spot) in dis tance 5 cm. Ef fi ciency of antisens ACC ox i dize gene on se lec tive me -dium con tain ing 150 mg/l kanamycin in di cated 16% (14 cotyledone em bryo) in dampit but in sarirona was not growth.Transforman growth on se lec tive me dium in di cated pGA643 SM4 have in serted to zy gotic em bryo be cause plasmidcon tain ing se lec tion gene of kanamycin (NPT II gene).
Co-Authors - Makful - Sunyoto Hary Subakti I Sukmayadi Kartono - Pudji Santoso R Iswanto Rahayu Triatminingsih Sri Hadiati Sri Handayani Subandi . T.I.R. Utami Y Meldia Yuniarti .