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All Journal Jurnal Fitopatologi Indonesia beta (Biosistem dan Teknik Pertanian) GEMA TEKNOLOGI Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Vektora : Jurnal Vektor dan Reservoir Penyakit VISIKES Prosiding KPSDA JURNAL BIOLOGI INDONESIA E-Dimas: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Jurnal Surya Masyarakat Jurnal Entomologi Indonesia Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Surya Masyarakat E-Amal: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Vokasi
Susiana Purwantisari
Biotechnology Laboratory, Departement Of Biology, Faculty Of Science And Mathematics, Diponegoro University
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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HUBUNGAN HIGIENE SANITASI DENGAN KUALITAS MIKROBIOLOGIS PADA MAKANAN GADO-GADO DI KECAMATAN TEMBALANG KOTA SEMARANG Yuniatun, Tuti; Martini, Martini; Purwantisari, Susiana; Yuliawati, Sri
Jurnal Kesehatan Masyarakat (Undip) Vol 5, No 4 (2017): JULI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.8 KB) | DOI: 10.14710/jkm.v5i4.18673

Abstract

Gado-gado is one of traditional foods from Indonesia which sold in Tembalang District. Gado-gado is made from various vegetables mixed with peanut sauce, served at temperature 5-60oC which is the "temperature danger zone" for any food. This situation makes gado-gado such a watery yet not-so-hot food when its served, so has a potency to be contaminated by microbes and fungis. The purpose of this research is to understand correlation between hygiene sanitation and microbiological quality of gado-gado in Tembalang District, Semarang City. This research is observational research using cross sectional design and consists of three variables, namely sellers health condition, sellers hygienic practice, and places sanitation. The samples of this research are 36 gado-gado and respondents of this research are 36 gado-gado sellers which is observed their hygienic sanitation while processing gado-gado. Data were analyzed by frequency distribution and bivariate analysis using chi square. The results showed that sellers health condition categorized as good condition (88,9%); sellers hygienic practice categorized as good condition (33,3%); and places sanitation categorized as good condition (22,2%). There was statistically significant correlation between sellers hygienic practice (p=0,008) and places sanitation (p=0,028) with gado-gado microbiological quality. While there is no corrrelation between sellers health condition (p>0,999) with gado-gado microbiological quality.Gado-gado sellers should pay attention to their personal hygiene and the places sanitation when processing gado-gado, to improve gado-gado microbiological quality.
Isolation and Identification of Mold Contaminants on Mushroom Growing Medium (Bag Log) and Their Cellulolytic Performance Test Handayani, Tatik; Purwantisari, Susiana
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 2 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.241 KB)

Abstract

Mushrooms naturally grows on logs that have experienced weathering. Raw materials of mushroom growth media containing plenty of wild microbes, especially wild mold on sawdust media. Sterilization conducted did not prevent the occurrence of contamination. This study aim was to determine the types of contaminant molds in cultivated mushroom growth media (bag logs), as well as determine their cellulolytic performance. Mushroom growth media (bag log) samples were taken from three mushroom cultivation locations. Mold isolates obtained were identified by their macroscopic and microscopic characteristics. Test the mold cellulolytic performance was conducted by measuring the ratio of hydrolysis zone with the diameter of mold on CMC (Carboxy Methyl Cellullose) media. The isolation and identification results of contaminant molds consisted of mold isolates which could be grouped into 7 genus which were Aspergillus, Penicillium, Paecillomyces, Trichoderma, Rhizopus, Fusarium and Syncephalastrum. The cellulolytic performances of mold on CMC media showed that Aspergillus flavus has the greatest cellulolytic ability.
The Potential Test of Fungal Antagonist Trichoderma viride to inhibit the Growth of Pathogenic Fungi Fusarium moniliforme and Alternaria solani In-Vitro Purwantisari, Susiana; Evendi, Agus
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 3 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.695 KB)

Abstract

Fusarium moniliforme and Alternaria solani are two types of mold which often cause the diseases of cultivated plants. Fusarium moniliforme causes the ear rot disease on corn and the wilt disease of Solanaceae family. Whereas the pathogenic fungus Alternaria solani causes an early bright disease on the onion and potato. This aim of this study was to determine the ability of fungal antagonist Trichoderma viride in inhibiting the growth of Fusarium moniliforme and Alternaria solani in vitro. The growth inhibition ability test were conducted on dual cultures by growing the fungal antagonists with pathogenic fungi in Petri dish containing potato dextrose agar media face-to-face in a distance of 3 cm. Percentages of the growth inhibiting were observed every day in 7 days incubation. The results showed that the fungal antagonist T. viride exhibited the highest inhibition on F. moniliforme in 3 days incubation period which was 63.07 %. Yet the highest inhibition against A. solani was in 2 days incubation period which was 57.35 %. T. viride growth continued to increase since the first day until the seventh day incubation period but contrarily the growth of both pathogenic fungi underwent inhibition. This suggested that T. viride was potential as a biological control agent of F. moniliforme and A. solani growth and have a potency as an active bio fungicide ingredient. Keywords: Dual culture; percentage inhibiting; Trichoderma viride; Alternaria solani; Fusarium moniliforme
Uji Bakteriologis Air Sambungan Rumah dengan Metode Most Probable Number (MPN) Quanti-Tray di PDAM Kabupaten Magelang Harwika Ria Rizky Agista; Susiana Purwantisari
Jurnal Akademika Biologi Vol. 9 No. 1 Januari 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.955 KB)

Abstract

Air merupakan kebutuhan penting bagi manusia. Air berasal dari alam yang harus melalui tahapan pengolahan hingga air layak dikonsumsi oleh masyarakat. Syarat air layak konsumsi secara bakteriologis adalah dengan ada tidaknya bakteri Coliform dan E. coli dalam air. Air layak konsumsi didistribusikan oleh PDAM dengan sistem perpipaan atau sambungan rumah. Penelitian ini bertujuan untuk mengetahui kualitas air Sambungan Rumah yang didistribusikan oleh PDAM Kabupaten Magelang melalui uji bakteriologis. Metode penelitian yang digunakan adalah secara eksperimental melalui pengujian bakteri Coliform dan E. coli menggunakan metode Most Probable Number (MPN) Quanti-Tray dengan sampel air Sambungan Rumah pada 5 daerah. Hasil penelitian diperoleh nilai MPN air Sambungan Rumah pada daerah A dan B belum memenuhi syarat air bersih yang siap digunakan masyarakat karena masih terdapat Coliform dan E. coli pada air Sambungan Rumah tersebut, sedangkan pada air Sambungan Rumah daerah C, D, dan E sudah memenuhi syarat air bersih secara bakteriolgis karena tidak terdapat bakteri Coliform dan E. coli dalam air Sambungan Rumah
Aplikasi Jamur Antagonis Trichoderma viride Terhadap Pengurangan Intensitas Serangan Penyakit Hawar Daun Serta Hasil Tanaman Kentang Purwantisari, Susiana
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
Publisher : Prosiding KPSDA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.744 KB)

Abstract

The  objective  of  this  research was  to  studi  ability  of Trichoderma sp. fungal antagonist on reduction leaf blight intensity disease and potato yield. The in vivo experiment was carried  out  at  potato  land  area  located  at  BALITSA in Cikole Sub District, Lembang  District and Bandung Regency. Completely  Randomized  Design  was  used  with  six treatment and five replicates. The treatment tested consist of control  (without pathogen fungal and antagonists fungus application too), control (with  pathogen fungal application and without antagonist fungal application), chemical fungicide application, antagonist fungal application 2 weeks before planting, application 1 week after planting and both application 2 weeks before and 1 week after planting. Result of  the  research  showed  that  application of antagonists fungal could decrease disease intensity and tended to improve crop potato yield. Research repeatment with more quantity pouring the antagonist was needed. These antagonist could be used as biological agents initials to control leaf blight disease.
Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid) Hanafi, Ahmad; Purwantisari, Susiana; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.909 KB) | DOI: 10.14710/bioma.19.1.76-82

Abstract

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different. Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH
Interaksi Kapang Patogen Fusarium oxysporum dengan Bakteri Kitinolitik Rizosfer Tanaman Jahe dan Pisang Ferniah, Rejeki Siti; Pujiyanto, Sri; Purwantisari, Susiana; Supriyadi, Supriyadi
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.542 KB) | DOI: 10.31258/jnat.14.1.56-60

Abstract

Fusarium oxysporum is a pathogenic fungi for many plants. The fungi have chitin cell wall that can be degraded by chitinase fromchitinolytic bacteria. Aim of this research is determine how the interaction between the bacteria and F.oxysporum. Bacteria were isolatedfrom plant rizosfere. Chitinolytic activity were measured based on the clear zone around the colony in chitin medium. Bacteria and fungiinteraction were determined by an antagonistic test. This research showed that there were 9 chitinolytic bacteria. J4 and P3 had highchitinolytic index, that are 3 and 3.33, respectively. The two isolates antagonist to F.oxysporum, which the bacteria prevent growth of thefungi. The J4 and P3 are alternative biofungicide for F.oxysporum.
Viabilitas dan jumlah produk mikoriza Kelompok Tani Ngudi Makmur di Desa Kataan Kecamatan Ngadirejo Temanggung Ahmad Arfi Setiadi; Susiana Purwantisari
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.702 KB) | DOI: 10.14710/jbt.2.2.80-84

Abstract

Mycorrhiza is microbes that provide positive benefits for plants. The purpose of this study was to determine the cell number and viability of mycorrhizae product of Ngudi Makmur Temanggung Farmer Group. The study was conducted from April to May 2018. The parameters of this study were viability and number of mycorrhiza cells in vitro at PDA medium. The method used inthis research is a direct calculation method for Petri dish containing media PDA then incubated for 0,4,8 and 12 days later. The calculation of mycorrhiza using the method indirectly grown on PDA media then calculated with the formula MPN. The microbial viability was calculated by comparing the number of microbes on the x-day incubation with the initial amount of incubation. The results showed that the highest number of colonies on mycorrhiza on the first day of 94 colonies / g and the number decreased constantly with increasing days so that at the last observation the number was 63 colonies / g. The viability of mycorrhiza tended to decrease with increasing incubation time. The viability of mycorrhiza tends to decrease with increasingincubation time.
Desa Sukodono adalah salah satu wilayah di Kabupaten Demak yang endemik filariasis (angka rata-rata 1,39%). Penemuan kasus baru dan mikrofilaria pada tubuh penderita kronis meningkatkan potensi terjadinya penularan dan infeksi mikrofilaria pada nyamuk. Pe Mawarni, Atik; Purwantisari, Susiana; Hestiningsih, Retno; Martini, Martini; Puspitasari, Elsye Giovanny
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 9 No 2 (2019): April
Publisher : LPPM STIKES KENDAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.123 KB) | DOI: 10.32583/pskm.9.2.2019.165-174

Abstract

Desa Sukodono adalah salah satu wilayah di Kabupaten Demak yang endemik filariasis (angka rata-rata 1,39%). Penemuan kasus baru dan mikrofilaria pada tubuh penderita kronis meningkatkan potensi terjadinya penularan dan infeksi mikrofilaria pada nyamuk. Penelitian ini bertujuan untuk mengetahui populasi Culex sp sebagai vektor filariasis. Penelitian ini adalah survei deskriptif dengan desain cross sectional. Sampel pada 155 rumah tangga. Nyamuk dikumpulkan menggunakan metode pengumpulan pendaratan dan pengumpulan istirahat pada pukul 6 sore - 6 pagi. Pengamatan lingkungan dilakukan untuk mengidentifikasi kondisi lingkungan (tempat berkembang biak dan tempat peristirahatan) Culex sp. Hasil penelitian menunjukkan bahwa spesies Culex ditangkap di desa Sukodono termasuk Cx. quinquefasciatus (58,5%), Cx. vishnui (35,8%), Cx. tritaeniorhychus (4,2%). Tingkat infeksi 0%. Sedangkan dari parsial Cx quinquefasciatus 12%, Cx. vishnui 13%, dan Cx. tritaeniorhychus 36%. Rata-rata suhu dan kelembaban 33.190C dan 67,93%. Ada tempat berkembang biak dan tempat istirahat berupa semak (69,7%), rawa (83,2%), beras (100%), genangan air (53,3%) dan ternak (unggas (93,5%); kambing (11%)) yang mendukung kepadatan nyamuk. Kepadatan tertinggi dari larva ditemukan di parit (1,4 larva). Proses penularan filariasis di Desa Sukodono masih terjadi, sehingga perlu kewaspadaan dan peningkatan upaya pencegahan filariasis. Bagi masyarakat, kebutuhan untuk meningkatkan upaya pengendalian vektor dan perbaikan lingkungan.   Kata kunci: Filariasis, Culex, Angka Infeksi, Parousitas   POPULATION OF CULEX SP AS VECTORS OF FILARIASIS   ABSTRACT Sukodono village is one of area in Demak district witch was endemic of filariasis (mf rate 1,39%). The discovery of new cases and microfilaria on chronic sufferers? body raises the potential occurrence of transmission and infection microfilaria on mosquitoes. This research aims to know population of Culex sp as vectors of filariasis. This study was an descriptive survey by cross sectional design. The sample was on 155 households. Mosquito was collected using a method of landing collection and resting collection at 6 pm - 6 am. Environmental observations performed to identify environmental conditions (breeding places and resting places) of Culex sp. The results showed that the species of Culex were caught in Sukodono village including Cx. quinquefasciatus (58,5%), Cx. vishnui (35,8%), Cx. tritaeniorhychus (4,2%). Infection rate 0%. While of paroucity of Cx quinquefasciatus 12%, Cx. vishnui 13%, and Cx. tritaeniorhychus 36%. The average of temperature and humidity 33,190C and 67,93%. There are breeding places and resting places in the form of shrubs (69.7%), swamp (83.2%), rice (100%), puddle (53.3%) and livestock (poultry (93.5%); goat (11%)) that support mosquito density.  The highest density of larvae found in ditch (1,4 larvas). The process of transmission of filariasis in Sukodono village was still happened, so that it takes vigilance and improvement of filariasis prevention efforts.  For the community, the need to enhance the efforts of vector control and environmental improvement.   Keywords: Filariasis, Culex, Infection Rate, Paroucity
HUBUNGAN SANITASI DENGAN STATUS BAKTERIOLOGI (STATUS Koliform DAN KEBERADAAN Salmonella sp) PADA JAJANAN DI SEKOLAH DASAR WILAYAH KECAMATAN TEMBALANG, SEMARANG Kumalasari, Ririh Citra; Martini, Martini; Purwantisari, Susiana
Jurnal Kesehatan Masyarakat (Undip) Vol 4, No 3 (2016): MEI
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.232 KB) | DOI: 10.14710/jkm.v4i3.12816

Abstract

Foodborne disease adalah suatu penyakit yang disebabkan oleh adanya mikroorganisme patogen yang masuk bersama makanan. Produk pangan yang tidak memenuhi persyaratan mutu, keamanan dan cemaran bakteri patogen dapat mengakibatkan terjadinya foodborne disease. Anak sekolah merupakan usia yang rentan terhadap infeksi bakteri dan membutuhkan makanan yang cukup secara kuantitas serta kualitas sehingga memiliki keadaan atau status gizi yang baik dan dapat memperkuat sistem imun dalam tubuhnya. Penelitian ini bertujuan untuk mengetahui hubungan sanitasi dengan kontaminasi bakteri pada makanan jajanan  di kantin sekolah dasar yang berada Kecamatan Tembalang Kota Semarang. Jenis penelitian analitik observasional dan pendekatan cross sectional. Sampel penelitian sejumlah 48 jajanan yang dijual di sekolah dasar. Pemeriksaan kontaminasi bakteri didasarkan angka koliform dan Salmonella sp. Data dianalisis dengan Chi-squre test. Hasil penelitian menunjukkan jajanan di kantin sekolah dasar yang tidak memenuhi syarat kesehatan sebesar 82,2% dengan status koliform tidak memenuhi syarat sebanyak 67,8% dan terkontaminasi Salmonella sp sebanyak 37,8%. Faktor yang berhubungan dengan kontaminasi bakteri dan status bakteriologi jajanan di sekolah dasar diantaranya adalah pengetahuan penjual, praktik sanitasi penjual, serta sanitasi tempat berjualan. Pendidikan kesehatan tentang pengelolaan makanan perlu diberikan pada penjual makanan di sekolah dasar.
Co-Authors Achmadi Priyatmojo Agus Evendi, Agus Ahmad Arfi Setiadi Ahmad Hanafi Ahmad Thontowi Ana Bila Nadia Anindya Ardiansari Arina Tri Lunggani Atik Mawarni Azizah, Hana Fitria Budi Raharjo Budi Raharjo Budihardjo, Kadarwati Dewi Fajar Kharisma Dwi Handayani Dwi Handayani Elvi Yetti, Elvi Fiva Andriyani Govinda Restu Syahputra Harum Sitepu Harwika Ria Rizky Agista Herry Wirianata Ilyas Teguh Pangestu Indriana Putri Inggrit Amedia, Inggrit Kadarwati Budiharjo Karnoto, K Lintang Dian Saraswati M. Arie Wuryanto Martini Martini MG Isworo Rukmi Mohamad Endy Y Yulianto Muhammad Bismar Iskandar putra Naufal, Mochammad Fa’iq Qoys Novi Astriana Nurul Halwiyah Pangestu, Ilyas Teguh Puspitasari, Elsye Giovanny Rejeki Siti fe Rejeki Siti Ferniah Retno Hestiningsih RETNO PENI SANCAYANINGSIH Retno Peni Sancayaningsih Rina Sri Kasiamdari Ririh Citra Kumalasari, Ririh Citra Rizka Amalia Rizky Wahyu Saputro Romario Dion Sarjana Parman Septa Primananda Sitepu, Harum Siti Nur Jannah Sofatun Misrofah Sri Pujiyanto Sri Yuliawati Sri Yuliawati Sri Yuliawati Sukarman Sukarman Suprihadi Suprihadi Supriyadi Supriyadi Sutra Diyana Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Tatik Handayani, Tatik Theresia Damayanti Tuti Yuniatun, Tuti Wijanarka Wijanarka YOPI YOPI Yudanto, Yusuf Arya