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Aplikasi Jamur Antagonis Trichoderma viride Terhadap Pengurangan Intensitas Serangan Penyakit Hawar Daun Serta Hasil Tanaman Kentang Purwantisari, Susiana
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
Publisher : Prosiding KPSDA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.744 KB)

Abstract

The  objective  of  this  research was  to  studi  ability  of Trichoderma sp. fungal antagonist on reduction leaf blight intensity disease and potato yield. The in vivo experiment was carried  out  at  potato  land  area  located  at  BALITSA in Cikole Sub District, Lembang  District and Bandung Regency. Completely  Randomized  Design  was  used  with  six treatment and five replicates. The treatment tested consist of control  (without pathogen fungal and antagonists fungus application too), control (with  pathogen fungal application and without antagonist fungal application), chemical fungicide application, antagonist fungal application 2 weeks before planting, application 1 week after planting and both application 2 weeks before and 1 week after planting. Result of  the  research  showed  that  application of antagonists fungal could decrease disease intensity and tended to improve crop potato yield. Research repeatment with more quantity pouring the antagonist was needed. These antagonist could be used as biological agents initials to control leaf blight disease.
Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1056.931 KB) | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic
Desa Sukodono adalah salah satu wilayah di Kabupaten Demak yang endemik filariasis (angka rata-rata 1,39%). Penemuan kasus baru dan mikrofilaria pada tubuh penderita kronis meningkatkan potensi terjadinya penularan dan infeksi mikrofilaria pada nyamuk. Pe Hestiningsih, Retno; Puspitasari, Elsye Giovanny; Martini, Martini; Mawarni, Atik; Purwantisari, Susiana
Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Vol 9 No 2 (2019): April
Publisher : LPPM STIKES KENDAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.123 KB) | DOI: 10.32583/pskm.9.2.2019.165-174

Abstract

Desa Sukodono adalah salah satu wilayah di Kabupaten Demak yang endemik filariasis (angka rata-rata 1,39%). Penemuan kasus baru dan mikrofilaria pada tubuh penderita kronis meningkatkan potensi terjadinya penularan dan infeksi mikrofilaria pada nyamuk. Penelitian ini bertujuan untuk mengetahui populasi Culex sp sebagai vektor filariasis. Penelitian ini adalah survei deskriptif dengan desain cross sectional. Sampel pada 155 rumah tangga. Nyamuk dikumpulkan menggunakan metode pengumpulan pendaratan dan pengumpulan istirahat pada pukul 6 sore - 6 pagi. Pengamatan lingkungan dilakukan untuk mengidentifikasi kondisi lingkungan (tempat berkembang biak dan tempat peristirahatan) Culex sp. Hasil penelitian menunjukkan bahwa spesies Culex ditangkap di desa Sukodono termasuk Cx. quinquefasciatus (58,5%), Cx. vishnui (35,8%), Cx. tritaeniorhychus (4,2%). Tingkat infeksi 0%. Sedangkan dari parsial Cx quinquefasciatus 12%, Cx. vishnui 13%, dan Cx. tritaeniorhychus 36%. Rata-rata suhu dan kelembaban 33.190C dan 67,93%. Ada tempat berkembang biak dan tempat istirahat berupa semak (69,7%), rawa (83,2%), beras (100%), genangan air (53,3%) dan ternak (unggas (93,5%); kambing (11%)) yang mendukung kepadatan nyamuk. Kepadatan tertinggi dari larva ditemukan di parit (1,4 larva). Proses penularan filariasis di Desa Sukodono masih terjadi, sehingga perlu kewaspadaan dan peningkatan upaya pencegahan filariasis. Bagi masyarakat, kebutuhan untuk meningkatkan upaya pengendalian vektor dan perbaikan lingkungan.   Kata kunci: Filariasis, Culex, Angka Infeksi, Parousitas   POPULATION OF CULEX SP AS VECTORS OF FILARIASIS   ABSTRACT Sukodono village is one of area in Demak district witch was endemic of filariasis (mf rate 1,39%). The discovery of new cases and microfilaria on chronic sufferers? body raises the potential occurrence of transmission and infection microfilaria on mosquitoes. This research aims to know population of Culex sp as vectors of filariasis. This study was an descriptive survey by cross sectional design. The sample was on 155 households. Mosquito was collected using a method of landing collection and resting collection at 6 pm - 6 am. Environmental observations performed to identify environmental conditions (breeding places and resting places) of Culex sp. The results showed that the species of Culex were caught in Sukodono village including Cx. quinquefasciatus (58,5%), Cx. vishnui (35,8%), Cx. tritaeniorhychus (4,2%). Infection rate 0%. While of paroucity of Cx quinquefasciatus 12%, Cx. vishnui 13%, and Cx. tritaeniorhychus 36%. The average of temperature and humidity 33,190C and 67,93%. There are breeding places and resting places in the form of shrubs (69.7%), swamp (83.2%), rice (100%), puddle (53.3%) and livestock (poultry (93.5%); goat (11%)) that support mosquito density.  The highest density of larvae found in ditch (1,4 larvas). The process of transmission of filariasis in Sukodono village was still happened, so that it takes vigilance and improvement of filariasis prevention efforts.  For the community, the need to enhance the efforts of vector control and environmental improvement.   Keywords: Filariasis, Culex, Infection Rate, Paroucity
Interaksi Kapang Patogen Fusarium oxysporum dengan Bakteri Kitinolitik Rizosfer Tanaman Jahe dan Pisang Ferniah, Rejeki Siti; Pujiyanto, Sri; Purwantisari, Susiana; Supriyadi, Supriyadi
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.542 KB) | DOI: 10.31258/jnat.14.1.56-60

Abstract

Fusarium oxysporum is a pathogenic fungi for many plants. The fungi have chitin cell wall that can be degraded by chitinase fromchitinolytic bacteria. Aim of this research is determine how the interaction between the bacteria and F.oxysporum. Bacteria were isolatedfrom plant rizosfere. Chitinolytic activity were measured based on the clear zone around the colony in chitin medium. Bacteria and fungiinteraction were determined by an antagonistic test. This research showed that there were 9 chitinolytic bacteria. J4 and P3 had highchitinolytic index, that are 3 and 3.33, respectively. The two isolates antagonist to F.oxysporum, which the bacteria prevent growth of thefungi. The J4 and P3 are alternative biofungicide for F.oxysporum.
Masa Inkubasi Gejala Penyakit Hawar Daun Tanaman Kentang yang Diinduksi Ketahanannya oleh Jamur Antagonis Trichoderma viride Purwantisari, Susiana; Priyatmojo, Achmadi; Sancayaningsih, Retno Peni; Kasiamdari, Rina Sri
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.469 KB) | DOI: 10.14710/bioma.18.2.41-47

Abstract

Late blight disease caused by Phytophthora infestans, is probably the single most important disease of potatoes worldwide. Infected plants were quickly killed and were difficult for replanting, causing significant losses for the growers. Various control methods were examined including the use of biocontrol agents of Trichoderma spp.  The biocontrol potential of Trichoderma viride against potato late blight pathogen, Phytophthora infestans, were studied  under greenhouse conditions. The research objective was to determine the ability of biocontrol agents Trichoderma viride to delay late blight disease incidence on potato plants in the field. The in vivo experiment was carried out at potato land area located at BALITSA in Cikole Sub District, Lembang District and Bandung Regency. Completely Randomized Design was used with six treatment and five replicates. The treatment tested consist of control (without pathogen fungal and antagonists fungus application too), control (with pathogen fungal application and without antagonist fungal application), chemical fungicide application, antagonist fungal application 2 weeks before planting, application 1 week after planting and both application 2 weeks before and 1 week after planting. Result of the research showed that application of antagonists fungal could delay disease intensity until 14 days. These antagonist could be used as biological agents initials to control leaf blight disease. Key words: Biological control, Potato late blight, Phytophthora infestans, Trichoderma viride
Ketahanan Sistemik Tanaman Kentang Oleh Aplikasi PGPR Purwantisari, Susiana; Parman, Sarjana; handayani, Dwi; Karnoto, K
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.529 KB) | DOI: 10.14710/bioma.21.2.126-131

Abstract

Late blight disease caused by Phytophthora infestans, is probably the single most important disease of potatoes worldwide. Infected plants were quickly killed and were difficult for replanting, causing significant losses for the growers. Various control methods were examined including the use of biocontrol agents of PGPR.  The research objective was to determine the ability of PGPR product from Ngudi Makmur farmer group local to delay late blight disease incidence on potato plants in the field. The in vivo experiment was carried out at potato land area located at Kledung  Sub District, Kledung District and Temanggung Regency. Randomized Block Design (RBD) with five treatments was applied with five treatments each of which was placed in a different plot and each plot was filled with 40 plant treatments. On the first plot, no treatment was given (P1); the second plot was given chemical fertilizer (P2), the third plot was supplemented with PGPR one dose (P3), the fourth plot was PGPR two dose (P4), and the last was the fifth plot treated with GA hormone (P5). Result of the research showed that application of PGPR could delay disease intensity until 14 days. These antagonist could be used as biological agents initials to control leaf blight disease. There was an improvement in the quality of potato tubers harvested with the PGPR application compared to controls too. 
Optimasi Produksi Inulinase oleh Khamir Pichia manshurica DUCC Y-015 pada Tepung Umbi Dahlia (Dahlia variabilis Willd.) dengan Variasi Konsentrasi K2HPO4 dan Waktu Inkubasi Amedia, Inggrit; Wijanarka, Wijanarka; Purwantisari, Susiana
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (152.252 KB) | DOI: 10.14710/bioma.18.2.48-55

Abstract

Sugar national supply more and more decreases and can not meet the market needs. The research has been done to find alternative natural sweeteners including inulin from dahlia tubers (D. variabilis Willd). Dahlia tuber can produce 95% of yield of fructose syrup in an enzimatic reaction by inulinase (E.C.3.2.1.7). Inulinase is inductive enzyme that can be produce by P. manshurica. The production of fructose needs to be optimized to get optimum product. The optimization can be done by modifying the nutrient content in the medium such as K2HPO4 and variation of incubation time. The purpose of this study is to determine the concentration of K2HPO4 and optimum incubation time for P. manshurica. This research was conducted in Microbiology Laboratory, Biology Department, Faculty of Science and Mathematics Undip. The examined variable is the growth of yeast cell, inulinase activity, invertase, and the I/S ratio. This research was conducted experimentally using Randomized Complete Block Design (RCBD) factorial pattern with 2 factors, the first factor was the concentration of K2HPO4 (P), with concentration  level (g/L) of 0.5 (P1), 1.0 (P2), and 1.5 (P3). The second factor was incubation time (W) with 12 hours (W12), 18 hours (W18), and 24 hours (W24). Every treatment was repeated three times. The collected data were analyzed using ANOVA. If there was a treatment effect, it will be continued with Duncan test on 5% significance level. The result of analysis show that the highest cell growth and the maximum production of inulinase enzyme was in P3W24 occurs in P3W24 (K2HPO4 1.5 g/L and 24 hours incubation time) treatment at 0.428 IU, but efficient in P1W12 treatment as much as 0.365 IU. Keywords: Dahlia variabilis Willd., Inulinase, K2HPO4, Pichia manshurica DUCC Y-015, incubation time
Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid) Hanafi, Ahmad; Purwantisari, Susiana; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.909 KB) | DOI: 10.14710/bioma.19.1.76-82

Abstract

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different. Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH
The Potential Test of Fungal Antagonist Trichoderma viride to inhibit the Growth of Pathogenic Fungi Fusarium moniliforme and Alternaria solani In-Vitro Purwantisari, Susiana; Evendi, Agus
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 3 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Fusarium moniliforme and Alternaria solani are two types of mold which often cause the diseases of cultivated plants. Fusarium moniliforme causes the ear rot disease on corn and the wilt disease of Solanaceae family. Whereas the pathogenic fungus Alternaria solani causes an early bright disease on the onion and potato. This aim of this study was to determine the ability of fungal antagonist Trichoderma viride in inhibiting the growth of Fusarium moniliforme and Alternaria solani in vitro. The growth inhibition ability test were conducted on dual cultures by growing the fungal antagonists with pathogenic fungi in Petri dish containing potato dextrose agar media face-to-face in a distance of 3 cm. Percentages of the growth inhibiting were observed every day in 7 days incubation. The results showed that the fungal antagonist T. viride exhibited the highest inhibition on F. moniliforme in 3 days incubation period which was 63.07 %. Yet the highest inhibition against A. solani was in 2 days incubation period which was 57.35 %. T. viride growth continued to increase since the first day until the seventh day incubation period but contrarily the growth of both pathogenic fungi underwent inhibition. This suggested that T. viride was potential as a biological control agent of F. moniliforme and A. solani growth and have a potency as an active bio fungicide ingredient. Keywords: Dual culture; percentage inhibiting; Trichoderma viride; Alternaria solani; Fusarium moniliforme
Isolation and Identification of Mold Contaminants on Mushroom Growing Medium (Bag Log) and Their Cellulolytic Performance Test Handayani, Tatik; Purwantisari, Susiana
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 2 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.241 KB)

Abstract

Mushrooms naturally grows on logs that have experienced weathering. Raw materials of mushroom growth media containing plenty of wild microbes, especially wild mold on sawdust media. Sterilization conducted did not prevent the occurrence of contamination. This study aim was to determine the types of contaminant molds in cultivated mushroom growth media (bag logs), as well as determine their cellulolytic performance. Mushroom growth media (bag log) samples were taken from three mushroom cultivation locations. Mold isolates obtained were identified by their macroscopic and microscopic characteristics. Test the mold cellulolytic performance was conducted by measuring the ratio of hydrolysis zone with the diameter of mold on CMC (Carboxy Methyl Cellullose) media. The isolation and identification results of contaminant molds consisted of mold isolates which could be grouped into 7 genus which were Aspergillus, Penicillium, Paecillomyces, Trichoderma, Rhizopus, Fusarium and Syncephalastrum. The cellulolytic performances of mold on CMC media showed that Aspergillus flavus has the greatest cellulolytic ability.