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All Journal Jurnal Fitopatologi Indonesia beta (Biosistem dan Teknik Pertanian) GEMA TEKNOLOGI Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Natur Indonesia Jurnal Akademika Biologi Jurnal Kesehatan Masyarakat Vektora : Jurnal Vektor dan Reservoir Penyakit VISIKES Prosiding KPSDA JURNAL BIOLOGI INDONESIA E-Dimas: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi Jurnal Biologi Tropika NICHE Journal of Tropical Biology Jurnal Surya Masyarakat Jurnal Entomologi Indonesia Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Surya Masyarakat E-Amal: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Vokasi
Susiana Purwantisari
Biotechnology Laboratory, Departement Of Biology, Faculty Of Science And Mathematics, Diponegoro University
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Analisis Cemaran Kapang dan Khamir pada Jamu Serbuk Instan Jahe Merah dan Temulawak Romario Dion; Susiana Purwantisari
Berkala Bioteknologi Vol 3, No 2 (2020): Volume 3 Nomor 2 November 2020
Publisher : Berkala Bioteknologi

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Abstract

ABSTRACTInstant herbal medicine is a powder or granule form of herbal medicine that is usually made from sugar and spices which are combined with or without the addition of other food ingredients and food additives. Powdered herbal medicine has the advantage that it is practical and fast in serving, and has a relatively long shelf life. However, poor processing of herbal powder can lead to microbial contamination such as mold and yeast. This study aims to determine the presence of mold and yeast contamination contained in the red ginger and ginger powder herbs. The steps taken in this study were sampling, preparation of tools and materials, homogenization and sample dilution, and testing for mold and yeast levels. This research was conducted for 2 months from August to September 2020 at the Biotechnology Laboratory, Department of Biology, Faculty of Science and Mathematics, Diponegoro University. The results showed that the mold and yeast levels found in the red ginger powder herbal sample were 1 x 101 colonies / g, while the mold and yeast levels found in the ginger powder herbal medicine were 21 x 101 colonies / g. of the two samples have met the standards of BPOM RI Regulation Number 32 of 2019 so that it can be concluded that the red ginger and ginger powder herbs are still safe for consumption.Keyword :  Herbal powder, Contaminants, Molds and Yeasts
Viabilitas dan jumlah produk mikoriza Kelompok Tani Ngudi Makmur di Desa Kataan Kecamatan Ngadirejo Temanggung Ahmad Arfi Setiadi; Susiana Purwantisari
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.702 KB) | DOI: 10.14710/jbt.2.2.80-84

Abstract

Mycorrhiza is microbes that provide positive benefits for plants. The purpose of this study was to determine the cell number and viability of mycorrhizae product of Ngudi Makmur Temanggung Farmer Group. The study was conducted from April to May 2018. The parameters of this study were viability and number of mycorrhiza cells in vitro at PDA medium. The method used inthis research is a direct calculation method for Petri dish containing media PDA then incubated for 0,4,8 and 12 days later. The calculation of mycorrhiza using the method indirectly grown on PDA media then calculated with the formula MPN. The microbial viability was calculated by comparing the number of microbes on the x-day incubation with the initial amount of incubation. The results showed that the highest number of colonies on mycorrhiza on the first day of 94 colonies / g and the number decreased constantly with increasing days so that at the last observation the number was 63 colonies / g. The viability of mycorrhiza tended to decrease with increasing incubation time. The viability of mycorrhiza tends to decrease with increasingincubation time.
Isolation and characterization of lactic acid bacteria from Apis mellifera and their potential as antibacterial using in vitro test against growth of Listeria monocytogenes and Escherichia coli Indriana Putri; Siti Nur Jannah; Susiana Purwantisari
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1200.645 KB) | DOI: 10.14710/niche.3.1.26-34

Abstract

Food spoilage can caused by pathogenic bacteria and can cause disease (food-borne pathogens). Foods that are vulnerable to pathogenic bacteria are foods that contain lots of protein, such as meat, milk and eggs. Listeria monocytogenes and Escherichia coli are commonly found in fresh meat. The solution for the problem is microbiological preservation by using Lactic Acid Bacteria (LAB). The purpose of this study is to isolate LAB from Apis mellifera honey, find out its characteristics, and test for antibacterial growth of L. monocytogenes and E. coli. LAB have been isolated with pour plate. Morphological characterization have been done by observing the colonies macroscopically and microscopically. LAB have been enumerated by Total Plate Count (TPC). Antibacterial Test have been done by well diffussion method. Experimental design was using Completely Randomized Factorial Design (CRFD). Statistical analysis have been done by univariate test with SPSS 16.0. 1st factor is LAB, 2nd factor is pH of supernatant and 3rd factor is pathogenic bacteria. Total 9 isolates of LAB were obtained from A. mellifera honey. LAB population is 9.6 x 105 CFU/mL. The result of screening LAB antibacterial assay is 2 potential isolates, they are MA 3 and MA 4. The best supernatant of LAB that could inhibit the growth of L. monocytogenes and E. coli is MA 3 with average diameters 12,05 mm and 12,07 mm. Pathogenic bacteria that is more sensitive is E. coli, because of the antibacterial compound from LAB is composed by organic acid that can break down the outer membrane and lower the pH, so pathogenic bacterial cannot be adapted to acidic environment.
Isolation and characterization of lactic acid bacteria from Apis mellifera stomach and their potential as antibacterial using in vitro test against growth of Staphylococcus aureus and Salmonella typhimurium Ana Bila Nadia; Siti Nur Jannah; Susiana Purwantisari
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (711.05 KB) | DOI: 10.14710/niche.3.1.35-44

Abstract

Food contaminated by pathogenic bacteria is very dangerous to consume especially if the bacteria can produce toxins. Staphylococcus aureus andSalmonella typhimurium are two examples of pathogenic bacteria that cause demage to food because they produce enterotoxins that can cause food poisoning to consumers. The use biological agents as antibacterial agents can be used as a solution to overcome this. Lactic acid bacteria (LAB) can produce compounds thet can inhibit the growth of pathogenic bacteria in food. This study aims to obtain local LAB isolates from the stomach of Apis mellifera in Indonesia and determine their characteristics as potential biopreservatif foods, to test the inhibition of LAB bacteriocin against Staphylococcus aureus and Salmonella typhimurium in vitro. The isolation method was carried out using multilevel dilution and streak plates on MRSA media. Morphological identification was carried out macroscopically on BAL and microscopic colonies with gram staining. Antibacterial test using the well method. LAB isolation result obtained eight isolatates. Antibacterial activitytest results for cells, all isolates showed inhibition of   Staphylococcus aureus and Salmonella typhimurium, for further antibacterial testing using 2 potential isolates namely PAM 3 and PAM 4. The two isolates were further tested for antibacterial with cell free supernatant using two treatments namely pH acidic and neutral, both isolates showed antibacterial activity at acidic ph better than neutral ph, wheares for the bacteriosin analysis test all isolates had no inhibition.  
Strategi Pemasaran Serbuk Jamu Instan UMKM Tiga Dara di Masa Pandemi Covid 19 Susiana Purwantisari; Anindya Ardiansari; Siti Nur Jannah; Rizky Wahyu Saputro
Jurnal Surya Masyarakat Vol 4, No 2 (2022): Mei 2022
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.4.2.2022.154-159

Abstract

Empowerment of Micro, Small and Medium Enterprises (MSME), requires model empowerment, which includes increasing existing MSME to be more advanced in order to be able to compete tightly in free market competition. Since ancient times, empon empon has been proven to have medicinal properties as herbal medicine, which is not only used as a medicine but also as a beauty treatment. One of the MSME that processes empon-empon into instant herbal medicine is Tiga Dara MSME which is located at Jalan Urip Sumoharjo No. 45 East Ungaran Subdistrict, Semarang Regency. Instant herbal powders produced by UD Tiga Dara include instant powders of Ginger, Turmeric, Temulawak, Mahkota Dewa, Kencur etc. The importance of maintaining body immunity during the COVID-19 pandemic is closely related to the availability of instant herbal powder products with empon-empon raw materials, especially ginger. During this pandemic, the demand for instant herbal medicine powder has increased, but Tiga Dara MSME still have limitations in the sales system. The method used in this community service is in the form of training and mentoring MSME in making web and social media as a means of marketing instant herbal powder products. This service aims to assist MSME in marketing their products in order to improve the economy of Tiga Dara MSME. With the existence of the Web and social media as one of the media for product marketing, it is hoped that it can increase sales from MSME and can improve the community's economy so that it can achieve community welfare.
Strategi Pemasaran Serbuk Jamu Instan UMKM Tiga Dara di Masa Pandemi Covid 19 Susiana Purwantisari; Anindya Ardiansari; Siti Nur Jannah; Rizky Wahyu Saputro
Jurnal Surya Masyarakat Vol 4, No 2 (2022): Mei 2022
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.4.2.2022.154-159

Abstract

Empowerment of Micro, Small and Medium Enterprises (MSME), requires model empowerment, which includes increasing existing MSME to be more advanced in order to be able to compete tightly in free market competition. Since ancient times, empon empon has been proven to have medicinal properties as herbal medicine, which is not only used as a medicine but also as a beauty treatment. One of the MSME that processes empon-empon into instant herbal medicine is Tiga Dara MSME which is located at Jalan Urip Sumoharjo No. 45 East Ungaran Subdistrict, Semarang Regency. Instant herbal powders produced by UD Tiga Dara include instant powders of Ginger, Turmeric, Temulawak, Mahkota Dewa, Kencur etc. The importance of maintaining body immunity during the COVID-19 pandemic is closely related to the availability of instant herbal powder products with empon-empon raw materials, especially ginger. During this pandemic, the demand for instant herbal medicine powder has increased, but Tiga Dara MSME still have limitations in the sales system. The method used in this community service is in the form of training and mentoring MSME in making web and social media as a means of marketing instant herbal powder products. This service aims to assist MSME in marketing their products in order to improve the economy of Tiga Dara MSME. With the existence of the Web and social media as one of the media for product marketing, it is hoped that it can increase sales from MSME and can improve the community's economy so that it can achieve community welfare.
PENDAMPINGAN PERIZINAN BPOM PRODUK SUSU PASTEURISASI UKM KARYA BUMI BOYOLALI SEBAGAI STRATEGI PERLUASAN PASAR Susiana Purwantisari; Siti Nur Jannah; Anindya Ardiansari; Muhammad Endy Yulianto; Ilyas Teguh Pangestu
E-Amal: Jurnal Pengabdian Kepada Masyarakat Vol 1 No 3: September 2021
Publisher : LP2M STP Mataram

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Abstract

Susu adalah salah satu bahan makanan sumber hewani yang sangat penting bagi manusia dengan kandungan gizi yang tinggi dan lengkap. Sentra peternakan sapi perah terbesar di Provinsi Jawa Tengah terletak di Kabupaten Boyolali sehingga sebagai tempat yang tepat untuk produksi susu sapi segar dan produk diversifikasinya. Karena kandungan gizi yang tinggi dan lengkap produksi susu menjadi perhatian yang sangat penting bagi pemerintah untuk menjamin bahwa susu tersebut aman dikonsumsi seluruh konsumen. Salah satu bentuk jaminan yang dikeluarkan pemerintah adalah NIE (Nomor Izin Edar). Susu dan produk olahannya seperti susu pasteurisasi, es krim, yougurt sebelum diedarkan harus mendapat izin Edar dari BPOM (Badan Pengawasan Obat dan Makanan). Permasalahan yang dialami oleh UKM Karya Bumi saat ini adalah belum memiliki NIE untuk produk susu pasteurisasi dan es krimnya sehingga masih kesulitan dalam memasarkan produknya. Program dari pengabdian masyarakat ini bertujuan untuk membantu usaha UKM Karya Bumi yang berada di Kabupaten Boyolali untuk mendapatkan NIE dari BPOM. Metode yang digunakan untuk mencapai tujuan program yaitu memberikan sosialisasi tentang prosedur mendapatkan NIE dari BPOM dan melakukan pendampingan dalam mengurus pengajuan NIE untuk produk susu pasteurisasi dan es krim. Hasil dari kegiatan ini adalah usaha mitra mendapatkan penyuluhan tentang prosedur pembuatan produk susu pasteurisasi dan es krim yang aman dikonsumsi, higienis, sehat dan bersih sehingga UKM Karya Bumi mendapatkan surat izin produk layak edar yaitu berupa NIE.
Produksi Susu Pasturisasi MJ9 UMKM Karya Bumi, Mojosongo, Boyolali Bersertifikat MD Dwi Handayani; Susiana Purwantisari; Siti Nur Jannah; Anindya Ardiansari
Jurnal Pengabdian Vokasi Vol 1, No 4 (2020): Nopember 2020
Publisher : Sekolah Vokasi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.698 KB) | DOI: 10.14710/jpv.2020.9194

Abstract

Kegiatan Pengabdian Kepada Masyarakat Program PPPUD ini bertujuan untuk merintis dan membina UMKM Karya Bumi dalam mengatasi pemasaran susu hasil peternak di Mojosongo Boyolali. Mengingat banyaknya susu yang tidak terjual, maka sebagai alternative dengan dibuat susu pasturisasi dengan berbagai rasa yang mempunyai daya simpan lebih lama. Dengan diproduksinya sebagai susu pasturisasi ini akan meningkatkan nilai jual, daya simpan dan memperluas pemasarannya.Program juga bertujuan untuk mempercepat difusi teknologi dan manajemen UKM, serta mengembangkan proses link and match antara Perguruan Tinggi, UKM, Pemerintah dan masyarakat.Program ini diawali dengan perancangan dan pabrikasi alat produksi, utamanya adalah pasturizer dengan kapasitas 50 liter yang dilengkapi sinar UV agar produk yang dihasilkan steril. Adanya alat tersebut perlu diberikan bimbingan teknis kepada pegawai yang terlibat pada proses produksi. Disamping itu juga diberikan penyuluhan tentang produksi bersih dan halal. Guna menjamin kualitas produksinya maka diupayakan untuk mendapatkan sertifikat MD dari Balai Pengawasan Obat dan Makanan Provinsi Jawa Tengah. Untuk memenuhi persyaratan agar diperoleh sertifikat MD tersebut diperlukan pembinaan dan penataan ruang produksi dan perlengkapannya serta laboratorium yang diperlukan. Ruang produksi harus selalu steril dengan perlengkapan yang memadahi untuk keperluan produksi, pengepakan hingga siap untuk dipasarkan.Adanya program ini diharapkan dapat memberikan solusi dalam mengatasi masalah penjualan susu hasil ternak petani dan akan meningkatkan nilai jualnya yang berarti akan meningkatkan pendapatan peternak sapi perah yang ada. 
Co-Authors Achmadi Priyatmojo Agus Evendi, Agus Ahmad Arfi Setiadi Ahmad Hanafi Ahmad Thontowi Ana Bila Nadia Anindya Ardiansari Arina Tri Lunggani Atik Mawarni Azizah, Hana Fitria Budi Raharjo Budi Raharjo Budihardjo, Kadarwati Dewi Fajar Kharisma Dwi Handayani Dwi Handayani Elvi Yetti, Elvi Fiva Andriyani Govinda Restu Syahputra Harum Sitepu Harwika Ria Rizky Agista Herry Wirianata Ilyas Teguh Pangestu Indriana Putri Inggrit Amedia, Inggrit Kadarwati Budiharjo Karnoto, K Lintang Dian Saraswati M. Arie Wuryanto Martini Martini MG Isworo Rukmi Mohamad Endy Y Yulianto Muhammad Bismar Iskandar putra Naufal, Mochammad Fa’iq Qoys Novi Astriana Nurul Halwiyah Pangestu, Ilyas Teguh Puspitasari, Elsye Giovanny Rejeki Siti fe Rejeki Siti Ferniah Retno Hestiningsih RETNO PENI SANCAYANINGSIH Retno Peni Sancayaningsih Rina Sri Kasiamdari Ririh Citra Kumalasari, Ririh Citra Rizka Amalia Rizky Wahyu Saputro Romario Dion Sarjana Parman Septa Primananda Sitepu, Harum Siti Nur Jannah Sofatun Misrofah Sri Pujiyanto Sri Yuliawati Sri Yuliawati Sri Yuliawati Sukarman Sukarman Suprihadi Suprihadi Supriyadi Supriyadi Sutra Diyana Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Tatik Handayani, Tatik Theresia Damayanti Tuti Yuniatun, Tuti Wijanarka Wijanarka YOPI YOPI Yudanto, Yusuf Arya