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POLYCLONAL ANTIBODY UTILIZATION FOR DETECTION OF JEMBRANA ANTIGEN AT BALI CATTLE IN WEST SUMATRA PROVINCE Helmi Helmi; Endang Purwati; Ibnu Rahmadani; Didik Tulus Subekti
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15014

Abstract

The aims of this study were to detect Jembrana antigen with polyclonal antibody and to describe antigen distribution in the Bali cattle organ that positively infected with Jembrana disease. Spleens, lungs, and livers were harvested from 10 naturally infected Bali cattle whose infection was confirmed through positive Polymerase Chain Reaction (PCR) result for Jembrana virus. Immunohistochemistry test was performed using polyclonal antibodies produced in rabbits. The results showed that cells infected by Jembrana viruses displayed positive reaction with a reddish brown color. Immunohistochemistry methods using polyclonal antibodies can detect Jembrana antigens in the spleen, liver, and lung with the highest average detection score (P0.05) was found in spleen, followed by liver and lungs. There was significant difference in the distribution of Jembrana antigens between the spleen, liver, and lungs with spleen having the highest antigen density.
Evaluasi Kinerja Daerah Irigasi Jragung Kabupaten Demak Putri, Eka Wulandari Srihadi; Harisuseno, Donny; Purwati, Endang
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 6, No 1 (2015)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

Jragung irrigation Area is located in Demak Regency has decreasedin its performance. The result of existing analysis showed that there is an imbalance of irrigation water within it. Based on its performance analysis taken from regulation of the ministry of public works No. 32/PRT/M/2007, Jragung Irrigation area has been classified as poor and requiremore attention with 66.95% working performance only. Efforts to increase its performance is by rehabilitation priority scale with following the AHP (Analytical Hierarcy Process) methods. With its main priority is Jragung Dam and Main Canal of Jragung, subsequently Teluk Secondary Canal, Sugihwaras Secondary Canal, Jragung Secondary Canal, Karangsono Secondary Canal, Ngumpul Secondary Canal, Pamongan Secondary Canal and Panjen Secondary Canal respectively. Efforts to overcome the imbalance of irrigation water is by the alteration of cropping periods from November to December and conventional cropping methods to SRI (System Rice Intensification) methods could save 53.25 % of irrigation water. Keywords : Irrigation Performance, Water Balance, AHP, Priority Scale, SRI Method
Model-Model Pembangkitan Data Sintetis Untuk Curah Hujan Harian Di Wilayah Brantas Tengah Soetopo, Widandi; Limantara, Lily Montarcih; Sayekti, Rini Wahyu; Purwati, Endang; Chandrasasi, Dian; Ilham, Muhammad; Rahmadi, Agung
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 4, No 1 (2013)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

This research is for finding the suitable the synthetic data generating model for daily rainfall in the region of Middle Brantas River Basin in the East Java Province. There are 7 models being considered, 4 models for single-site generation, (1) the two-part, model group, (2) the transition probability matrix model group, (3) the resampling model group, and (4) the time series model group, and 3 models for multisite generation, (5) the conditional, model group, (6) the extension of single site Markov chain model group, and (7) the random cascade model group. All of the time-series produced by the daily rainfall synthetic data generation are then tested statistically. The results show that statistically the differences between the historical time series and the synthetically time series are not too significant. It turn out that the multisite model have produced better synthetic time series compared to those which have been produced by the single-site models.Keywords: generating model, synthetic data, daily rainfall.
UJI PREKLINIS VIRGIN COCONUT OIL TERHADAP PENINGKATAN KADAR KOLESTEROL BAIK HDL, PENURUNAN KADAR TRIGLISERIDA, PROFIL KIMIA ASAM LEMAK (C6-C18) DAN OMEGA-3 PADA SERUM DARAH TIKUS PUTIH (Mus musculus) Syukur, Sumaryati; Dahlyanti, Rina; Sumanti, Titi; Murni, Yulia; Hidayat, Zoni; Arifin, Helmi; Purwati, Endang; -, Risfaheri
Jurnal Riset Kimia Vol 1, No 1 (2007): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i1.79

Abstract

 ABSTRACT The Virgin Coconut Oil (VCO) products have different qualities and controversy effects of lipids metabolism. This research has been used one of VCO product that was produced by fermentation method using Lactobacillus sp. It has high amount of lauric acid (C12) up to 51 %, caprilic acid (C8) 8.9 %, capric acid (C10) 7 % include Omega-3, 6 and 9, vitamins A, D, E, K and three kinds of phytohormone. The ratio of Omega-3 and 6 was very proportional, therefore it is important to investigate the Pre Clinic Test of animal experiment. Pre Clinic Test of dietary VCO as food supplement has been determined by using 40 mice’s, which divided into 4 groups. Feeding on egg yolk to Group I (negative control), Group II (positive control) increased cholesterol level. The others were Group III (egg yolk and VCO 2 %) and group IV (egg yolk and VCO 4 %). It was determined the total of cholesterol, HDL at 10th, 20th and 30th day treated by using the enzymatic methods. The dietary of VCO 2-4 % resulted in significant increases in HDL levels from 32 % to 69 %. The dietary of VCO 4 % for four weeks did not toxic to mice metabolism. Triglycerides level decreased 50 % from 177 to 85 and similar resulted to cholesterol ratio. Feeding on VCO for 4 weeks, the SCFA and MCFA not detected in serum of mice. The LCFA (C16) palmitate in significant decreased from 0.96 to 0.1%. The significant level of Omega-3 increased more than three times in serum of mice dietary VCO 2-4 %.  Keywords: Coconut Oil, Lactobacillus
Purifikasi Parsial Enzim Ekstraseluler (Anoxybacillus sp.) yang Diisolasi dari Sumber Air Panas Bukit Kili Solok serta Aplikasinya untuk Menghidrolisis Limbah Berserat Octarya, Zona; Syukur, Sumaryati; Purwati, Endang
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.856 KB) | DOI: 10.31258/jnat.15.2.106-114

Abstract

Termostable enzyme from thermophilic bacteria is very potential to improve technical enzyme in industry which used hightemperature. High water temperature exerts selection pressure on microbial species leading to specific flora that survivesand tolerates heat stress. The relative isolation and unique physical properties of Bukit Kili Ketek Hot Springs in Solok,West Sumatera may yield unique thermophiles. The generation of extracellular enzymatic bacterial is highly desirable forproduction of hydrolitic enzymes, which are useful in various industrial application and in animal feeds. This study,conducted to purify extracellular enzymes from thermophilic bacteria (Anoxybacillus sp.). This bacteria was isolated inBukit Kili Ketek Hot Springs, and after identified by analysis of 16S rRNA gene, 97% of similiarty with Anoxybacillus sp.was of obtained. The temperature of the hot waters was 52°C and the pH was 8. Extracellular and hydrolytic enzymeproduction were screened by qualitative SDS-PAGE method. SDS-PAGE analysis gave protein bands at ±110 kDa, ±80 kDa,±60 kDa, 50 kDa, 25 kDa, and ±10 kDa, respectively. Extracellular enzymes were used to degrade cellulose waste. Thecellulose activity for degradation of baggasse and pineapple pulp was 0,451 IU/mL and 0,310 IU/mL at 50°C and pH 6.
Analisis Efektivitas Penambahan Kapasitas Pintu Air Manggarai Untuk Pengendalian Banjir Di Wilayah Sungai Ciliwung Anggraini, Nimas Ayu; Dermawan, Very; Purwati, Endang
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 6, No 1 (2015)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

DKI Jakarta Province consists of 5 cities and 1 district. At the north part of Jakarta there are beaches which became the estuary of 13 rivers and 2 canals. Ciliwung is the main river that has an effect on the lives of people in Jakarta. The Ciliwung river flows towards to BKB. Manggarai sluice gate is located at the upstream of BKB to control Ciliwung river flow. This study was conducted by giving two addition of Manggarai sluice gate, each dimension of additional gate is 3.4 m x 8.1 m and 5 m x 5.3 m. Discharge calculations for hydrology analysis used Log Pearson III while hydraulics analysis used software HECRAS 4.1.0. Discharge data at AWLR MT Haryono control point recorded on January 21st,2013 showed 287.876 m 3 /sec. From the data, sluice gate simulations conducted with 8 alternatives opening gates. In this study showed the most effective results for Q2th and Q5th with opening gates 2 m (alternative 4), Q10th with opening gates 4 m (alternative 5), Q20th and Q25th with opening gates 6 m (alternative 6), Q50 and Q100th with maximum gates opening (alternative 8).Keywords: Sluice Gate, Simulation, Gate Operation, Opening Gate
Model-Model Pembangkitan Data Sintetis Untuk Curah Hujan Harian Di Wilayah Brantas Tengah Soetopo, Widandi; Limantara, Lily Montarcih; Sayekti, Rini Wahyu; Purwati, Endang; Chandrasasi, Dian; Ilham, Muhammad; Rahmadi, Agung
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 6, No 1 (2015)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.741 KB)

Abstract

This research is for finding the suitable synthetic daily rainfall generating model in the Middle Brantas River Basin - East Java. There are 7 models being considered, 4 single-site models, (1) the two-part, (2) the transition probability matrix, (3) the resampling, and (4) the time series, and 3 multisite models, (5) the conditional, (6) the extension of single site Markov chain, and (7) the random cascade. All time-series produced by the models are then tested statistically. The results show that the differences between the historial time series and the synthetical time series are not too significant. It turn out that the synthetic time series of multisite models are better than the synthetic time series of single-site models.Keywords: generating model, synthetic data, daily rainfall.
UPAYA KONSERVASI LAHAN BERDASARKAN INDIKATOR EROSI DAN SEDIMEN DI DAS JRAGUNG taufiq, muhamad; Andawayanti, Ussy; Purwati, Endang
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 7, No 2 (2016)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

ABSTRAK: Daerah Aliran Sungai (DAS) Jragung terletak pada Wilayah Kerja Balai Besar Wilayah Sungai Pemali Juana. Pertambahan penduduk di DAS Jragung wilayah hulu menyebabkan perubahan pada fungsi lahan. Studi Upaya Konservasi Lahan Berdasarkan Indikator Erosi dan Sedimen di DAS Jragung sangat diperlukan untuk meminimalkan permasalahan di DAS Jragung. Pendugaan laju erosi dan sedimentasi dihitung dengan model AVSWAT 2000. Hasilnya menunjukkan besarnya limpasan permukaan, erosi dan sedimentasi sebesar 707,519 mm/thn, 168,51 ton/ha/th dan 229.806,089 ton/th. DAS Jragung memiliki indeks bahaya erosi rendah sebesar 40,19%, sedang sebesar 25,66%, tinggi sebesar 14,63%, dan sangat tinggi sebesar 19,52% terhadap luas DAS jragung Upaya konservasi secara vegetatif dapat mereduksi erosi sebesar 38,19% dan secara mekanis dapat mereduksi sedimen sebesar 48,09%. Kata-kata kunci: Limpasan permukaan, erosi, sedimentasi, AVSWAT 2000 ABSTRACT: Jragung Watershed (DAS) located in BBWS Pemali Juana. The population increase that occurred in the upper reaches Jragung watershed cause many changes in land use. The study of land conservation efforts based on indicators of erosion and sediment in Jragung watershed is necessary to minimize the problems that exist in Jragung watershed. The estimation of the rate of erosion and sedimentation is calculated by the model approach AVSWAT 2000. The results showed that the amount of surface runoff, erosion and sedimentation on the current state of each of 707.519 mm / yr, 168.51 tons / ha / year and 229.806,089 tons / year. Jragung watershed have erosion hazard index was lower by 40.19%, currently at 25.66%, higher by 14.63%, and was very high at 19.52% of the area jragung watershed. Conservation efforts for vegetatif erosion can reduce the amount of 38.19% and mechanically reduce sediment of 48.09%. Key words: surface runoff, erosion, sedimentation, AVSWAT 2000
SIMULASI INDEKS PENGGUNAAN AIR (IPA) GUNA PENGHEMATAN AIR IRIGASI DI D.I. SONOSARI DAN D.I. PAKIS KABUPATEN MALANG Sayekti, Rini Wahyu; Purwati, Endang; Ismoyo, M Janu
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 8, No 2 (2017)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.435 KB) | DOI: 10.21776/ub.pengairan.2017.008.02.10

Abstract

ABSTRAK : Daerah Irigasi Sonosari memiliki 12 petak tersier dengan luas total baku sawah total 801 ha serta saluran sekunder dengan panjang 12 km. Sedangkan Daerah Irigasi Pakis memiliki total luas baku sawah sebesar 726 ha. Dengan total luas baku sawah yang cukup besar serta tidak sesuainya antara kebutuhan air irigasi dengan pemberian air irigasi menyebabkan sering terjadi kekurangan pada musim kemarau. Berdasarkan permasalahan diatas, perlu adanya evaluasi sistem pemberian air irigasi di daerah tersebut dan salah satu caranya adalah dengan metode indeks penggunaan air (IPA) guna mendapatkan nilai debit yang efisien serta nilai FPR yang sesuai dengan jenis tanah.Dari hasil simulasi IPA didapatkan bahwa dengan mencoba-coba nilai IPA = 0,7 (kategori sedang) memiliki hasil terbesar yaitu 194,389 lt/dtk (Musim Hujan), 213,956 lt/dtk (Musim Kemarau I) dan 116,606 lt/dtk (Musim Kemarau II) pada intake. Untuk penghematan pemberian air irigasi dengan mencoba-coba nilai FPR didapatkan bahwa FPR = 0,12 memiliki hasil terbesar yaitu 363,009 lt/dtk (Musim Hujan), 500,620 lt/dtk (Musim Kemarau I) dan 275,346 lt/dtk (Musim Kemarau II) pada intake. Sementara di D.I. Pakis untuk penghematan pemberian air irigasi yang paling besar, yaitu pada efisiensi 55% di musim kemarau I sebesar 540,45 lt/dt dengan nilai IPA = 0,55 yang termasuk kategori sedang.  Sementara untuk penghematan pemberian air irigasi dengan nilai IPA dan efisiensi yang sama pada musim hujan didapatkan debit sebesar 486,45 lt/dt dan musim kemarau II sebesar 412,20 lt/dt. Kata Kunci: Indeks Penggunaan Air, Kebutuhan Air, Penghematan Air ABSTRACT : Sonosari Irrigation Region had 12 tertiary patches of rice field at 801 ha total width supported by secondary channel at 12 km length. Meanwhile Pakis irrigation area have a total of 726 ha. However, the width of the total area of rice fields as well as mismatches between system of water management with demand for water irrigation brought the consequences of water scarcity in the dry season. When water was needed, it overwhelmed the capacity of irrigation region. Water could not afford the remote patch of the rice field. By taking account the problem above, irrigation water system at Sonosari Irrigation Region must be re-evaluated. One way to do this re-evaluation was through water usage index (IPA) can be use for water saving and FPR score also indicated the compatibility of water usage with soil type.Result of IPA simulation indicated that the obtained IPA score was 0.7 (moderate category) with the biggest water production of 194.389 liters/second (Rain Season), 213.956 liters/second (Dry Season I) and 116.606 liters/second (Dry Season II) on intake. The simulation of irrigation water retrenchment by experimenting FPR scores had given some results. At FPR score of 0.12, there were the biggest water production rates derived at 363.009 liters/second (Rain Season), 500.620 liters/second (Dry Season I) and 275.346 liters/second (Dry Season II) on intake. Meanwhile in Pakis irrigation area found that for the provision of irrigation water savings are greatest, at the efficiency of 55% in the dry season I at 540.45 L/ sec to the value of the IPA = 0.55 in the medium category.  As for the provision of irrigation water savings to the value of IPA and the same efficiency in the wet season discharge obtained at 486.45 L/sec and dry season II amounted to 412.20 L/sec. Keywords: Water Usage Index, Water Demand, Water Retrenchment
POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO -, Urnemi; Syukur, Sumaryati; Purwati, Endang; Ibrahim, Sanusi; -, Jamsari
Jurnal Riset Kimia Vol 4, No 2 (2011): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i2.141

Abstract

 ABSTRACTLactic acid bacteria (LAB) were isolated from cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii.Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, That was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12. mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, antimicrobial activity, SDS-PAGE, cocoa fermentation  and food biopreservative