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POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO -, Urnemi; Syukur, Sumaryati; Purwati, Endang; Ibrahim, Sanusi; -, Jamsari
Jurnal Riset Kimia Vol 4, No 2 (2011): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i2.141

Abstract

 ABSTRACTLactic acid bacteria (LAB) were isolated from cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii.Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, That was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12. mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, antimicrobial activity, SDS-PAGE, cocoa fermentation  and food biopreservative    
UJI PREKLINIS VIRGIN COCONUT OIL TERHADAP PENINGKATAN KADAR KOLESTEROL BAIK HDL, PENURUNAN KADAR TRIGLISERIDA, PROFIL KIMIA ASAM LEMAK (C6-C18) DAN OMEGA-3 PADA SERUM DARAH TIKUS PUTIH (Mus musculus) Syukur, Sumaryati; Dahlyanti, Rina; Sumanti, Titi; Murni, Yulia; Hidayat, Zoni; Arifin, Helmi; Purwati, Endang; -, Risfaheri
Jurnal Riset Kimia Vol 1, No 1 (2007): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i1.79

Abstract

 ABSTRACT The Virgin Coconut Oil (VCO) products have different qualities and controversy effects of lipids metabolism. This research has been used one of VCO product that was produced by fermentation method using Lactobacillus sp. It has high amount of lauric acid (C12) up to 51 %, caprilic acid (C8) 8.9 %, capric acid (C10) 7 % include Omega-3, 6 and 9, vitamins A, D, E, K and three kinds of phytohormone. The ratio of Omega-3 and 6 was very proportional, therefore it is important to investigate the Pre Clinic Test of animal experiment. Pre Clinic Test of dietary VCO as food supplement has been determined by using 40 mice’s, which divided into 4 groups. Feeding on egg yolk to Group I (negative control), Group II (positive control) increased cholesterol level. The others were Group III (egg yolk and VCO 2 %) and group IV (egg yolk and VCO 4 %). It was determined the total of cholesterol, HDL at 10th, 20th and 30th day treated by using the enzymatic methods. The dietary of VCO 2-4 % resulted in significant increases in HDL levels from 32 % to 69 %. The dietary of VCO 4 % for four weeks did not toxic to mice metabolism. Triglycerides level decreased 50 % from 177 to 85 and similar resulted to cholesterol ratio. Feeding on VCO for 4 weeks, the SCFA and MCFA not detected in serum of mice. The LCFA (C16) palmitate in significant decreased from 0.96 to 0.1%. The significant level of Omega-3 increased more than three times in serum of mice dietary VCO 2-4 %.  Keywords: Coconut Oil, Lactobacillus
Studi Kelayakan Pembangunan Dermaga Pelabuhan Canti Rajabasa Kabupaten Lampung Selatan Andono, Rizky Harsya; Purwati, Endang; Sholichin, Mohammad
Jurnal Mahasiswa Jurusan Teknik Pengairan Vol 1, No 1 (2017)
Publisher : Jurusan Teknik Pengairan, Fakultas Teknik, Universitas Brawijaya

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Abstract

Pelabuhan Canti di Kecamatan Rajabasa, Kabupaten Lampung Selatan ini adalah salah satu pelabuhan rakyat yang dimana fungsi pelabuhannya akan ditingkatkan menjadi Pelabuhan Lokal yang berfungsi sebagai Pelabuhan Pengumpan Lokal sesuai dengan RencanabbIndukbPelabuhan Nasional No. KP 414 Tahun 2013 dan Peraturan Daerah KabupatenbLampung Selatan Nomor 15 Tahun 2012 tentang Rencana Tata Ruang Wilayah KabupatenbLampung Selatan 2011-2031, Pelabuhan Canti ditetapkan sebagai Pelabuhan Lokal yang berfungsi sebagai Pelabuhan Pengumpan Lokal. Sebagai Pelabuhan Pengumpan Lokal maka Pelabuhan Canti dari segi pelayanan ialah pelabuhan yang fungsinya melayani kegiatan angkutan laut dalam negeri, bongkar muat angkutan laut dalam negeri dalam jumlah terbatas, dan merupakan pengumpan bagi pelabuhan utama dan pelabuhan pengumpul, dan sebagai tempat asal tujuan penumpang dan/atau barang, serta angkutan penyeberangan dengan jangkauan pelayanan provinsi. Dengan kenaikan fungsionalitas pelabuhan serta kondisi eksisting Pelabuhan Canti yang tidak sesuai dengan keamanan dan keselamatan pelayaran menjadi dasar dari pekerjaan pembangunan proyek Pelabuhan Canti di Kecamatan Rajabasa, Kabupaten Lampung Selatan yang bertujuan untuk dapat memfasilitasi angkutan kebutuhan pokok masyarakat yaitu sandang, pangan dan papan, hasil bumi seperti pertanian, peternakan, perikanan dan layanan jasa pariwisata menuju alur pelayaran yang telah ditentukan.
SKRINING DAN IDENTIFIKASI BAKTERI TERMOFILIK PENGHASIL SELULASE DAN AMILASE DARI SUMBER AIR PANAS BUKIT KILI SOLOK SUMATERA BARAT DENGAN ANALISIS GEN 16S rRNA Octarya, Zona; Syukur, Sumaryati; RN, Endang Purwati
Sistem Informasi Vol 2 No 1 (2011): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

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Abstract

Enzim termostabil dari bakteri termofilik merupakan enzim yang sangat potensial untuk mengatasi kendala teknis industri yang berhubungan dengan proses pada suhu tinggi. Salah satu sumber enzim adalah mikroorganisme termofilik yang banyak terdapat pada sumber air panas. Indonesia merupakan negara kepulauan yang banyak mempunyai sumber air panas, salah satunya adalah Kabupaten Solok. Penelitian ini mencakup skrining dan identifikasi bakteri penghasil enzim termostabil selulase dan amilase dari sumber air panas Bukit Kili Ketek di Kabupaten Solok. Suhu air panas adalah 52°C pada bulan Juni 2009 dan pH air adalah 8. Kultur murni yang diperoleh adalah sebanyak 22 kultur Dari hasil pewarnaan gram, diperoleh sebagian besar bakteri air panas adalah jenis bakteri streptobacilli (basilus) gram positif, hanya dua isolat yang merupakan gram negatif yaitu isolat S1B dan S5B. S2A adalah isolat yang mempunyai aktifitas selulase paling tinggi dibandingkan dari isolat yang lain karena mempunyai indeks zona bening selulase lebih tinggi yaitu 2,6. Isolat S2A juga menghasilkan amilase tinggi dengan indeks zona bening 2,5. Persen identitas bakteri air panas S2A yang diperoleh adalah 97% dengan Anoxybacillus flavithermus strain AE3.
Response of Superovulation by Using FSH (Follicle Stimulating Hormone) and Sex Determination of Embryos Using PCR in Pesisir Cows of West Sumatra Afriani, Tinda; Sayful, Fery Lismanto; Sumedi, Sumedi; Putra, Dino Eka; Purwati, Endang
ANIMAL PRODUCTION Vol 20, No 1 (2018)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.453 KB) | DOI: 10.20884/1.jap.2018.20.1.630

Abstract

This study  was conducted to determine the response of superovulation by giving 16 ml dosage  of FSH hormone to female Pesisir cattle.  The estrus schedule of 15 Pesisir cows was set by inserting CIDR (Controlled Internal Drug Release) into the vagina for 12 days. At day 10, all cattles were injected with FSH for three consequent days but with decreasing dosage. On the 3rd day, FSH injection was accompanied by PGF2α injection and CIDR was removed. The detection of estrus was performed at  day 13. Natural mating was proceeded after the estrus signs visible. Collection of donor embryos was done on the 6th and 8th day after mating. The variables  measured were the response of superovulation, total number of corpus luteum, number of embryos and sex ratio. The results obtained were all  Pesisir cows responded to superovulation. The  average number of of corpus luteum and embryoes per cow were  5.93±3.17 and -----, respectively,  while the total of transferable embryoes were 90, with an average of 6.00 or 61.64%. The sexing of embryoes obtained in this study from 146 embryoes awere 76.03% males (111 embryoes) and 23.97% females (35 embryoes). Based on total of transferable embryoes, there were 51.37% male embryos and 11.28% of females embryos. The result of this study showed that the sex ratio of male embryos was higher than female embryos.
POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO -, Urnemi; Syukur, Sumaryati; Purwati, Endang; Ibrahim, Sanusi; -, Jamsari
Jurnal Riset Kimia Vol 1, No 2 (2008): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i2.149

Abstract

ABSTRACT Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative                                                      
The Effect of Pediococcus pentosaceus on Stool Frequency, TNF-α Level, Gut Microflora Balance in Diarrhea-induced Mice Yuliawati, Yuliawati; Jurnalis, Yusri Dianne; Purwati, Endang; Lubis, Gustina
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 13, NUMBER 2, August 2012
Publisher : The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.24871/132201297-102

Abstract

Background: Enteropathogenic Escherichia coli (EPEC) are pathogenic microorganisms causing inflammation and imbalanced gut microflora that may result in diarrhea. Pediococcus pentosaceus (P. pentosaceus) isolated from “dadih” (milk curd) are used as probiotics containing lactic acid bacteria (LAB), which are useful to improve the balance of intestinal microflora and inhibit the growth of pathogenic microorganisms. This study was aimed to recognize the effect of P. pentosaceus supplementation on stool frequency, tumor necrosis factor-α (TNF-α) and gut microflora balance in experimental mice with EPEC-induced diarrhea. Method: The study was conducted in 60 white mice (Mus muscullus) at Biomedical Laboratory, Biotechnology/Production and Animal Husbandry Technology Institute, University of Andalas, Padang in April 2012. The frequency of stool, TNF-α level and microflora balance of the mice were measured before and after the EPEC-induced diarrhea and following the administration of antibiotics. Statistical analysis was performed using ANOVA and Duncan test. Results: The highest mean stool frequency was found in positive control group, i.e. 55 times, which was reduced significantly after 12-hour P. pentosaceus supplementation in a dose of 2 x 108 cfu/g into 18 times. The mean TNF-α level in positive control group was 128.17 pg/mL that lowered significantly to 48.0 pg/mL. The highest mean total number of LAB was 97.0 x 107 cfu/g, which was significantly different from positive control group of 7 x 107 cfu/g. Conclusion: P. pentosaceus supplementation in a dose of 2 x 108 cfu/g may reduce the stool frequency, lower TNF-α and improve the gut microflora balance following 12-hour supplementation in diarrhea-induced mice. Keywords: Pediococcus pentosaceus, TNF-α, diarrhea, EPEC, gut microflora
Model-Model Pembangkitan Data Sintetis Untuk Curah Hujan Harian Di Wilayah Brantas Tengah Soetopo, Widandi; Limantara, Lily Montarcih; Sayekti, Rini Wahyu; Purwati, Endang; Chandrasasi, Dian; Ilham, Muhammad; Rahmadi, Agung
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 4, No 1 (2013)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

This research is for finding the suitable the synthetic data generating model for daily rainfall in the region of Middle Brantas River Basin in the East Java Province. There are 7 models being considered, 4 models for single-site generation, (1) the two-part, model group, (2) the transition probability matrix model group, (3) the resampling model group, and (4) the time series model group, and 3 models for multisite generation, (5) the conditional, model group, (6) the extension of single site Markov chain model group, and (7) the random cascade model group. All of the time-series produced by the daily rainfall synthetic data generation are then tested statistically. The results show that statistically the differences between the historical time series and the synthetically time series are not too significant. It turn out that the multisite model have produced better synthetic time series compared to those which have been produced by the single-site models.Keywords: generating model, synthetic data, daily rainfall.
Analisis Efektivitas Penambahan Kapasitas Pintu Air Manggarai Untuk Pengendalian Banjir Di Wilayah Sungai Ciliwung Anggraini, Nimas Ayu; Dermawan, Very; Purwati, Endang
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 6, No 1 (2015)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

DKI Jakarta Province consists of 5 cities and 1 district. At the north part of Jakarta there are beaches which became the estuary of 13 rivers and 2 canals. Ciliwung is the main river that has an effect on the lives of people in Jakarta. The Ciliwung river flows towards to BKB. Manggarai sluice gate is located at the upstream of BKB to control Ciliwung river flow. This study was conducted by giving two addition of Manggarai sluice gate, each dimension of additional gate is 3.4 m x 8.1 m and 5 m x 5.3 m. Discharge calculations for hydrology analysis used Log Pearson III while hydraulics analysis used software HECRAS 4.1.0. Discharge data at AWLR MT Haryono control point recorded on January 21st,2013 showed 287.876 m 3 /sec. From the data, sluice gate simulations conducted with 8 alternatives opening gates. In this study showed the most effective results for Q2th and Q5th with opening gates 2 m (alternative 4), Q10th with opening gates 4 m (alternative 5), Q20th and Q25th with opening gates 6 m (alternative 6), Q50 and Q100th with maximum gates opening (alternative 8).Keywords: Sluice Gate, Simulation, Gate Operation, Opening Gate
Evaluasi Kinerja Daerah Irigasi Jragung Kabupaten Demak Putri, Eka Wulandari Srihadi; Harisuseno, Donny; Purwati, Endang
Jurnal Teknik Pengairan: Journal of Water Resources Engineering Vol 6, No 1 (2015)
Publisher : Fakultas Teknik, Universitas Brawijaya

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Abstract

Jragung irrigation Area is located in Demak Regency has decreasedin its performance. The result of existing analysis showed that there is an imbalance of irrigation water within it. Based on its performance analysis taken from regulation of the ministry of public works No. 32/PRT/M/2007, Jragung Irrigation area has been classified as poor and requiremore attention with 66.95% working performance only. Efforts to increase its performance is by rehabilitation priority scale with following the AHP (Analytical Hierarcy Process) methods. With its main priority is Jragung Dam and Main Canal of Jragung, subsequently Teluk Secondary Canal, Sugihwaras Secondary Canal, Jragung Secondary Canal, Karangsono Secondary Canal, Ngumpul Secondary Canal, Pamongan Secondary Canal and Panjen Secondary Canal respectively. Efforts to overcome the imbalance of irrigation water is by the alteration of cropping periods from November to December and conventional cropping methods to SRI (System Rice Intensification) methods could save 53.25 % of irrigation water. Keywords : Irrigation Performance, Water Balance, AHP, Priority Scale, SRI Method