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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya Majalah Kesehatan FKUB
Anik Puryatni
Laboratorium Ilmu Kesehatan Anak Rumah Sakit Umum Dr. Saiful Anwar Malang
Agriculture, Biological Sciences & Forestry Environmental Science Medicine & Pharmacology Public Health

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Efek Supresi Curcumin pada Organogenesis dan Morfogenesis Embrio Ayam Umur 48 Jam Kusumaningrum, Estiani; Rahayu, Indriati Dwi; Puryatni, Anik
Majalah Kesehatan FKUB Vol 2, No 4 (2015)
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.979 KB)

Abstract

Perkembangan embrio diiringi dengan peningkatan nutrisi, oksigenasi serta pembuangan zat sisa metabolisme sel. Peningkatan tersebut merangsang sel-sel mesenkimal  untuk menginduksi sel endotel menjadi jaringan pembuluh darah, proses ini disebut vaskulo-angiogenesis. Sistem vaskuler yang terbentuk sempurna akan memfasilitasi proses organogenesis dan morfogenesis embrio berjalan tanpa ada gangguan. Adanya gangguan dapat menyebabkan defek kongenital. Kurkumin sering digunakan sebagai bahan pengawet makanan dan obat–obatan di berbagai lapisan masyarakat. Walau sudah menjadi konsumsi harian, penelitian mengenai keamanan kurkumin terhadap embrio masih terbatas dan belum menjelaskan pengaruhnya terhadap kondisi fetal. Penelitian ini mengamati pengaruh kurkumin pada proses vaskulo-angiogenesis yang berpengaruh pada proses organogenesis secara in ovo pada embrio ayam. Konsentrasi kurkumin yang digunakan adalah 12,5 µM, 25 µM dan 50 µM. Kurkumin diinjeksikan pada telur ayam berusia kurang dari 1 hari dan kemudian diinkubasi selama 48 jam. Hasil penelitian menunjukan bahwa kurkumin dapat menghambat organogenesis pada embrio ayam dengan hasil uji ANOVA yang bermakna (p < 0,05). Pada sirkulasi yolk sac, berdasarkan uji Chi square diketahui hasilnya signifikan ( p < 0,05). Kurkumin juga berpengaruh dalam retardasi proses flexi cranial, pada perkembangan sistem optikus dan penutupan neuropore anterior berdasarkan umur embrio ayam dengan hasil uji Chi square yang bermakna (p < 0,05). Penelitian ini menyimpulkan bahwa kurkumin dapat menghambat proses perkembangan vaskulo-angiogenesis sehingga menyebabkan hambatan pada proses organogenesis embrio. Kata Kunci : Curcumin, Embrio, in ovo, Organogenesis,Vaskulogenesis.
ENCEPHALOPATY AKUT PADA ANAK DENGAN KELAINAN METABOLISME BAWAAN (PENDEKATAN PRAKTIS) Puryatni, Anik; de Koning, Tom J.
Jurnal Kedokteran Brawijaya Vol 24, No 3 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.983 KB) | DOI: 10.21776/ub.jkb.2008.024.03.7

Abstract

ABSTRACT Acute  encephalopathy  is  a  not  a  common,  but  a  potential  emergency  situation  in  newborns  and  youngchildren.  If  the  encephalopathy  is  not  caused  by  asphyxia  or  infections  of  the  brain,  rare  inborn  errors  ofmetabolism need to be considered. An acute encephalopathy due to an inborn error can occur in newborns,young  infants,  or  even  in  childhood  and  is  not  always  easily  recognized  because  more  subtle  presentationscan  occur.  In  this  article  we  will  give  a  practical  guideline  to  the  diagnostic  approach  of  metabolic  diseasespresenting with acute neurological symptoms with an emphasis on treatable disorders and the application indeveloping  countries  with  limited  diagnostic  resources.  The  first  step  in  the  evaluation  of  patient  with  apossible  metabolic  disease  is  to  categorise  the  clinical  appearance  of  the  patient  in  one  of  the  followingclinical categories: Hypoglycemia phenotype; Intoxication phenotype; Neurotransmitter defect phenotype; andCellular energy  metabolism defect  phenotype.  Second, combine this clinical classification with your physicaexamination  and  the  result  of  routine  laboratory  investigations  and  this  can  lead  to  suspected  diagnosis  ofmetabolic  disorders.Prompt  recognition  and  treatment  are  important,  because  an  acute  metabolicencephalopathy can irreversible and interruption of normal neural activity in the developing brain can have along-lasting  effect  on  psychomotor  development.  Delay  in  diagnosis  and  treatment  may  thus  result  in  acutemetabolic decompensation, progressive neurologic injury, or even death.  Key words: acute encephalopathy, inborn error metabolisme
Pengaruh Substitusi Tepung Tempe pada F100 terhadap Saturasi Transferin Puryatni, Anik
Jurnal Kedokteran Brawijaya Vol 26, No 2 (2010)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.17 KB) | DOI: 10.21776/ub.jkb.2010.026.02.7

Abstract

ABSTRAKPermasalahan status gizi di Indonesia masih merupakan permasalahan kesehatan serius dengan 28.47% anak berada pada  kondisi  gizi  kurang  dan  gizi  buruk.  Upaya  untuk  mengembangkan  bahan  pangan  bergizi  dan  terjangkau  sangat diperlukan. Penelitian ini dilakukan untuk membuktikan dampak pemberian susu skim dengan substitusi tepung tempe dalam  memperbaiki  status  gizi  pada  anak  dengan  malnutrisi.  Penelitian  dilakukan  dengan  desain  uji  klinis  pada  dua kelompok  yang  dipilih  secara  acak,  yaitu  kelompok  dengan  suplemen  standar  WHO  dan  kelompok  dengan  substitusi tepung  tempe.  Data  menunjukkan  peningkatan  signifikan  berat  badan  pada  kedua  kelompok.  Saturasi  tranferin meningkat sebesar 13.02+7.87 pada kelompok dengan pemberian suplemen standar WHO F100, sedangkan kelompok dengan  substitusi  tepung  tempe  meningkat  sebesar  12.47+5.317.  Tidak  ada  perbedaan  signifikan  peningkatan  berat badan antar dua kelompok. Hal ini menunjukkan bahwa pemberian substitusi dengan tempe kedele dapat memberikan efek yang sama dengan suplemen standar WHO F100 pada anak dengan dengan malnutrisi.Kata Kunci: Anak, berat badan, F100, malnutrisi, saturasi transferin, tepung tempe
KADAR INSULIN-LIKE GROWTH FACTOR-1 (IGF-1) AIR SUSU IBU DAN HUMAN BETA DEFENSIN-2 (HBD-2) FESES TERHADAP LUARAN KLINIS NEONATUS Kurniawan, Bayu; Puryatni, Anik; Sujuti, Hidayat
Majalah Kesehatan FKUB Vol 7, No 1 (2020): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.199 KB) | DOI: 10.21776/ub.majalahkesehatan.2020.007.01.2

Abstract

Prevalensi sepsis neonatal meningkat 40% pada prematuritas, bukti klinis menunjukkan etiologi translokasi bakteri pencernaan. American Academy of Pediatrics merekomendasikan pemberian ASI pada neonatus prematur. ASI berkontribusi pada fungsi pertahanan pencernaan melalui growthfactors, antimicrobial protein and peptides,dan sitokin.Growth factors ASI yang signifikan pada maturitas usus neonatus adalahIGF-1; IGF-1menunjukkan efek protektif selama peradangan pencernaan. Diagnosis definitif infeksi pencernaan pada neonatus prematur sulit ditegakkan. Pemeriksaan dini biomarker feses yang non-invasifmenjadi prioritas, peptida yang teridentifikasi pada feses neonatus dan berhubungan dengan inflamasi adalah HBD-2. Penelitian tentang pola kadar IGF-1ASI dan HBD-2 feses neonatus sebagai biomarker inflamasi terbatas. Tujuan penelitian ini untuk mengetahui pola kadar IGF-1ASItransisi dan HBD-2feses neonatus terhadap luaran klinis neonatus. Sampel penelitian adalah 24 pasangan ibu dan neonatus yang dikumpulkan pada Bulan Juni-Agustus 2019di ruang neonatologi RSSA Malang. Sampel dikelompokkan menjadi 4 kelompok berdasarkan usia kehamilannya. Karakteristik pasangan ibu-neonatus dan luaran klinis neonatus dikumpulkan. Kadar IGF-1ASItransisi dan HBD-2 feses diukur dengan metode ELISA dan kemudian dilakukan analisis statistik. Hasil penelitian menunjukkan perbedaan bermakna meankadar IGF-1ASI transisi dan HBD-2 feses dari tiap kelompok (p<0,05); kadar IGF-1 ASI transisi berbanding lurus dengan usia kehamilan (r=0,611) dan sebaliknya kadar HBD-2 feses (r=-0,725) (p<0,05).Terdapat perbedaan kadar HBD-2 feses yang bermakna pada luaran klinis patologis neonatus (feeding intolerance, enterokolitis nekrotikan, dan penggunaan ventilasi mekanik) (p<0,05).Disimpulkan bahwa prematuritas menunjukkan kadar IGF-1 ASI transisi yang rendah dan peningkatan kadar HBD-2 feses, sehingga meningkatkan risiko infeksi saluran pencernaan.  
Cysteine, Malondyaldehide (MDA) and Glutathione (GSH) Levels in Marasmic Type Malnutrition Cahyani, Dwi Indah; Puryatni, Anik; Permatasari, Nur
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.11

Abstract

Micronutrien deficiency in severe malnutrition will reduce antioxidant capacity that needed for oxidative stress defense. Cysteine, a non-essential amino acid, is one of an important component for reduced glutha-tione (GSH). This study aims to prove the difference between the levels of cysteine, MDA and GSH levels in children with marasmic malnutrition and well-nourished children and prove whether there is a relationship between those parameters. Fiftysix patients participated in this study were grouped into two groups of sam-ples that were marasmic type malnutrition group (28 patients) and control groups that were well nourished group (28 patients). Examination begins with a complete laboratory screening, followed by examination of cysteine, MDA and GSH level. Of the 28 patients included in marasmic type malnutrition group consisting of 15 male patients (53.6%) and 13 female patients (46.4%), while the well-nourished group consisted of 13 male patients (46.4%) and 15 female patients (53.6%). The average age is 54.61±56.35 months in the group of ma-rasmic type malnutrition and 48.25±45.34 months in the well-nourished group. By using the Mann Whitney test, there were significant difference between the levels of cysteine and GSH in marasmic malnutrition and control group (p = 0.000 and p = 0.000 respectively). Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels in marasmic malnutrition children shows no significant correlation (R = -0.206, p = 0.294; R = -0.036, p = 0.856; R = 0.210, p = 0.284 respectively). In well-nourished group Spearman correlation test between cysteine and GSH levels, cysteine and MDA levels, MDA and GSH levels also shows no significant correlation (R = -0.053, p=0.789; R = -0.146, p = 0.458; R = -0.079, p = 0.688 re-spectively). From this study it can be concluded that there are significant differences of the levels of cysteine and GSH between severe malnutrition groups compared with the control one. But this study shows no signif-icant correlation between the levels of cysteine and GSH levels in marasmic type malnutrition and well-nourished children.
Relationship between Cysteine, Interleukin (IL)-2, And Interleukin (IL)-10 in Children with Marasmus Type Malnutrition Wahyudi, Teguh; Puryatni, Anik; Hernowati, Tinny Endang
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.10

Abstract

 Malnutrition is one of the health issues in developing countries. The most commonly found malnutrition is the marasmus type. Infection in marasmus patient is the main cause of morbidity and mortality in developing countries. In marasmus, there is a decrease in protein level such as cysteine which is one of the glutathione forming components that plays a significant role in immune system. In malnutrition, there is a disturbance of lymphocyte in the form of down-regulation of type 1 cytokine (IL-2 and IFN-γ) and up- regulation of type 2 cytokine (IL-4 and IL-10). IL-2 is needed for the development of regulatory T produced by thymus and for NK cell cytotoxicity which plays a role in infection process, while IL-10 inhibits activation of lymphocyte T so the cellular immunity reaction ends. Several studies about the relationship between cysteine, IL-2, and IL-10 have been done in malnutrition-patients, but there is no study focusing on patients with marasmus. This study is to find out the relationship between cysteine, IL-2, and IL-10 in patients with marasmus type malnutrition. This study was an observational analytic study using cross-sectional design consisting of 20 children with marasmus type malnutrition and 20 well-nourished children treated in Saiful Anwar Hospital Malang. The cysteine, IL-2, and IL-10 level then measured using Elisa method. Normality and the various test were done. The Pearson correlation test was done to find out the relationship between cysteine and IL-2 level, cysteine and IL-10 level, and IL-2 and IL-10. The standard of cysteine and IL-2 level in children with marasmus is significantly lower than the control group, which was 1.616 ± 1.039 vs 3.298 ± 0.519 pg/mL; p = 0.000 dan 12.38±4.94 vs. 16.58 ± 4.80 pg/mL;p = 0.010, respectively. IL-10 in children with marasmus was significantly higher than control group (19.08± 5.93 vs 10.46 ± 3.90 pg/mL; p = 0.000). The cysteine level was positively correlated to the IL-2 level (p = 0.000; r = 0.71), while the cysteine and IL-10, IL-2 and IL-10 was negatively correlated with p = 0.014; r= -0.53 and p=0.037; r= -0.46 in marasmus. There was a positive correlation between the cysteine and IL-2 level and negative correlation between IL-10 and IL-2 also IL-2 and IL-10 in children with marasmus. 
Low Levels of Vitamin D correlate with Hemoglobin A1c and Interleukin-10 Levels in Pediatric Type 1 Diabetes Mellitus Patients Wulandari, Desy; Cahyono, Haryudi A.; Widjajanto, Edi; Puryatni, Anik
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Co-Authors Bayu Kurniawan Cahyani, Dwi Indah Cahyono, Haryudi A. desy wulandari Edi Widjajanto Estiani Kusumaningrum, Estiani Hidayat Sujuti Indriati Dwi Rahayu Nur Permatasari Teguh Wahyudi Tinny Endang Hernowati Tom J. de Koning