Claim Missing Document
Check
Articles

Found 3 Documents
Search

PROPORTION OF ISOMORPHIC ERYTROCYTE URINE IN DIABETIC KIDNEY DISEASE WITH FLOW CYTOMETRY METHODS Erica Catarina; Coriejati Rita; Basti Andriyoko; Ida Parwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1480

Abstract

Hematuria can be found in diabetic kidney disease. Urinary erythrocytes morphology can differentiate hematuria in diabetic kidney disease from other glomerular disorders. Different etiologies need different management. Urinalysis with flowcytometry method can directly give information about urine erythrocyte morphology which is not obtained by the conventional method. The aim of this study was to determine the proportion of urinary isomorphic erythrocytes in diabetic kidney disease. This was a descriptive cross-sectional study in the Dr. Hasan Sadikin Hospital Bandung from July 2016 to July 2017. Subjects were 38 patients who have been diagnosed as diabetic kidney disease by clinicians and had hematuria. Random urine samples were collected for erythrocytes morphology assay by using flowcytometry method and u-ACR values by using spectrophotometry method. The result of this study was 57.9% male, with the most frequent age were 55-64 years old group (34.2%) and 63.2% from all subject were included in the macroalbuminuria category. In erythrocyte morphology assay, 84.2% was isomorphic erythrocyte which 83.3% was macroalbuminuria group. The proportion of hematuria in diabetic kidney disease with automated integrated urine flowcytometry method was dominated by isomorphic erythrocyte morphology. Isomorphic erythrocytes in DM did not mean absence of glomerular abnormalities.
HoSPITAL ACQuIReD PneuMonIA onSeT DAN BAKTEREMIA Bellya Affan Roes; Dewi Kartika; Basti Andriyoko
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.466

Abstract

Hospital acquired pneumonia (HAP) is significantly impact the patient morbidity and mortality that cause etiologic identification becomes a microbiological emergency in HAP. The etiologic identification based on the onset of pneumonia is important to determine the specific pathogens, that impact patient prognosis and prevent bacteremia. The aim of the study is to know the bacterial profile and antibiotic susceptibility pattern of early onset HAP by determination, late onset HAP and HAP with bacteriaemia in the intensive care setting. The design of this study was retrospective descriptive. The data was collected from the Clinical Microbiology Laboratory, Department of Clinical Pathology and the medical record from Dr. Hasan Sadikin hospital Bandung, from April 2013–March 2013. From the 61 episodes of HAP, 18 were early onset HAP and 43 were late onset HAP, including five (5) related to (8.2%) HAP with bacteriaemia. Klebsiella pneumoniae is the most common etiology of the early onset HAP (22.2%), with the highest susceptibility (75%) to amikacin, meropenem, and tigecyclin. Acinetobacter baumannii is the most common etiology of the late onset HAP (27.9%), with the highest susceptibility (75%) to amikacin and cotrymoxazole. The most common etiology of HAP with bacteriaemia is Klebsiella pneumoniae confirmed ESBL (40%) with the highest susceptibility to amikacin and meropenem (100%). Based on this study, it can be concluded that the most common bacterial profile of the early onset HAP is K. pneumoniae, while for the late onset HAP is A. baumannii, and HAP with bacteriaemia is K. pneumoniae confirmed ESBL. All of them have the highest susceptibility to amikacin.
PERBANDINGAN KADAR HEMOGLOBIN ANTARA METODE SPECTROPHOTOMETER DENGAN METODE HEMOCUE PADA SAMPEL LEUKOSITOSIS Basti Andriyoko; Leni Lismayanti; Delita Prihatni
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.975

Abstract

Background and objective: The measurement of haemoglobin was carried out by using hematology analyzer with specthrophotometermethod which measured light absorbance at 540 nm. However, this measurement affected by increase turbidity as a result of leucocytosis.The turbidity can be eliminated by using HemoCue method that detect absorbance at 570 nm and 880 nm. The aim of this study wasto compare the measurement of haemoglobin obtained between specthrophotometer method and HemoCue method. Materials andmethod: Blood EDTA sample that have been measured with MEK-6318K Nihon Kohden hematology analyzer for haemoglobin levelswith spectrophotometer methods with leucocyte > 100.000/mm3 were included in this study. Blood sample are measured again forthe haemoglobin level with HemoCue B-Haemoglobin Analyzer. This study was conducted at Clinical Pathology Laboratory, Dr. HasanSadikin Hospital Bandung from August–October 2008. Result: Seventeen leucocytosis sample were enroled in this study. The meanhaemoglobin level from specthrophotometer method is higher than HemoCue method, however there was no statistically significantdifference between haemoglobin result from specthrophotometer method and HemoCue method (p = 0.742 > a = 0.05). Conclusion:There was no significant difference beetween specthrophotometer method and HemoCue method for haemoglobin measurement ofleucocytosis sample.