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All Journal Biosaintifika: Journal of Biology & Biology Education Berkala Penelitian Hayati
Endang Semiarti
Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Developmental the pattern of embryogenic microspore of rice (Oryza sativa L.) based on morphological characteristic Siti Nurbaiti; Yekti Asih Purwestri; Budi Setiadi Daryono; Endang Semiarti; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 1 (2019): December 2019
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.554 KB) | DOI: 10.23869/18

Abstract

Microspore culture is a rapid approach to get double haploid plants in shorten time which is important for plant breeding. Microspore culture of pigmented rice cultivar Segreng was used to observe the pattern of embryogenic development based on the characteristics of morphology. Characterization between embryogenic and non-embryogenic microspores was conducted after exposed anther to carbohydrate starvation in B medium culture at 33oC for 4 days. The result showed that enlarged microspore and star-like structure of fragmented cytoplasm become a marker of embryogenic microspore after stresses treatment. More complex of fragmented microspore was found at 4 days in A2 medium and developed further into multicellular structure. These multicellular structures might be originated either by symmetrical or asymmetrical division. The longer incubation, the higher frequency of complex fragmented microspore that developed into multicellular structure release from exine has appeared.
Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum) Arkan Setiaji; RR Rifka Annisa; Rumiyati Rumiyati; Endang Semiarti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Abstract

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Co-Authors Ari Indrianto Arkan Setiaji Budi Setiadi Daryono RR Rifka Annisa Rumiyati Rumiyati Siti Nurbaiti Yekti Asih Purwestri