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ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI Escherichia coli DH5a Ni Nyoman Tri Puspaningsih; Akhmaloka; Sofyan Hadi; Y. Sri Wulan Manuhara; Bambang Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 4 No 2 (1999): June 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/517

Abstract

Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10% higher than transformant control (Escherichia coli DH5a which content YCp50).
CONTAMINATION OF Pb, Cd, Cu, Zn IN BLACK POMFRET (Formio niger) AND TONGUE SAND (Cynoglossus lingua) FISH CAUGHT FROM THE WESTERN PART OF MADURA STRAIT AND ITS SAFE LIMITS CONSUMPTION Acivrida Mega Charisma; Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.14 KB) | DOI: 10.23869/143

Abstract

Contamination of Pb, Cd, Cu, Zn in the flesh on the two types of fish consisting pelagic fish include Black pomfert (Formio niger) whereas among other demersal fish include Tongue sand (Cynoglossus lingua), was conducted in March-December 2013, which was taken from the western part of Madura Strait (Gresik-Ujung pangkah, Surabaya-Kenjeran, and Sidoarjo-Sedati). Pb, Cd, Cu, and Zn detected using AAS (Atomic Absorption Spectrometry) brand Shimadzu AA-6200. The highest content of heavy metals, namely Pb in Cynoglossus lingua from the Sedati (5.63ppm±0.05), while a low of Cd in Formio niger from the Ujung pangkah (0.20ppm±0.004). The results show that there is a correlation between heavy metal with fish but not significantly. The content of Pb, Cd, Cu, Zn in 2 fish species originating from all locations stated below the exposure limits for consumption. Safe limit fish consumption of Formio niger is based on the WHO PTWI of 347.43 grams / week and Cynoglossus lingua 191.25 grams / week.
KEKAYAAN JENIS PORTUNIDAE DI SISI SHIPPING LINE SELAT MADURA Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/378

Abstract

A monitoring activity for the existence of portunid crabs in the narrowest part of Madura Strait has been done from 2001 to 2004. Crabs have been collected in June or July each year in day time during spring tide. Crab collection was carried out in four sampling station (I to IV) using 0,5 cm trawl net for 10 minutes netting in each time. The results show that during four year monitoring six species of portunid crab has been captured. Among the six species three of them, Charybdis affinis, C. anisodon, and Portunus pelagicus were collected each year even with difference level in local distribution. C. affinis and P. pelagicus have been monitored in the same three stations, and C. anisodon has also been monitored in three stations but differs from other three portunid crab species, C. annulata. P. trituberculatus, and Scylla paramamosain were very rare and have been captured in less then three sampling times collection. We conclude that in this area the portunid crab has been dominated by C. affinis which have less economic value, and for C. anisodon even the number higher than other portunid genus the occurrence is not stabil as in C. affinis.
POLA PENIJAHAN UDANG AIR TAWAR Macrobrachium sintangense De Man: PENGAMATAN SEMUSIM DI RANU KLINDUNGAN PASURUAN Bambang Irawan; Indinah Indinah; Hani Sudarmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 2 (2000): June 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/475

Abstract

The spawning pattern of Macrobrachium sintangense (de man) population in Klindungan pond (Ranu Klindungan) was observed. The number of eggs carrying female was count and high proportion of these female was found in August and February. This result indicate that there was two period of spawning in this population.
TOLERANSI UDANG REGANG Macrobrachium sintangense TERHADAP SALINITAS Bambang Irawan; Indinah; Hani Sudarmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 2 No 2 (1996): December 1996
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/485

Abstract

Experiment on salinity tolerance in Macrobrachium sintangense was conducted. The result showed that Macrobrachium sintangense has a wide salinity tolerance and all individuals could survive in the experimental medium up to 20 ppt salinity level, but no individuals could survive in the 35 ppt salinity more than 24 hours.
KANDUNGAN LOGAM NON ESENSIAL (Pb, Cd dan Hg) DAN LOGAM ESENSIAL (Cu, Cr dan Zn) PADA SEDIMEN DI PERAIRAN TUBAN GRESIK DAN SAMPANG JAWA TIMUR Moch. Syaifullah; Yuniar Ade Candra; Agoes Soegianto; Bambang Irawan
Jurnal Kelautan Vol 11, No 1 (2018)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v11i1.4497

Abstract

Logam berat seperti Pb, Cd, Hg, Cu, Cr dan Zn diperairan akan turun kemudian mengendap membentuk sedimen, beberapa organisme yang mencari makan didasar perairan akan mempunyai peluang besar untuk terpapar logam berat ini. Penelitian ini bertujuan untuk mengetahui kandungan logam berat non esensial Pb, Cd, Hg dan logam berat esensial Cu, Cr, Zn pada sedimen di perairan Tuban, Gresik dan Sampang. Kandungan logam berat pada sedimen ini menggunakan Atomic Absorption Spectrometry (AAS). Hasil penelitian menunjukkan bahwa kandungan logam non esensial timbal (Pb) yakni 3,3687 ± 0,5257 mg/kg-1 di Gresik, 2,9443 ± 0,3608 mg/kg-1 di Tuban dan 3,4253 ± 0,3697 mg/kg-1 di Sampang. Kandungan Kadmium (Cd) 2,978 ± 0,224 mg kg-1 di Tuban, 2,955 ± 0,179 mg kg-1 di Gresik, 3,012 ± 0,148 mg kg-1 di Sampang. Kandungan merkuri (Hg) 0,000029 ± 0,000012 mg kg-1 di Tuban, 0,000033 ± 0,000012 mg kg-1 di Gresik, 0,000034 ± 0,000045 mg kg-1 di Sampang. Adapun hasil analisis Kandungan logam esensial. Kandungan Tembaga (Cu) 0,0780 ± 0,0069 mg/kg-1 di Tuban, 0,0740 ± 0,0118 mg/kg-1 di Gresik dan 0,0750 ± 0,0069 mg/kg-1 di Sampang. Kandungan Kromium (Cr) 0,000033 ± 0,0000045 mg/kg-1 di Tuban, 0,000034 ± 0,0000011 mg/kg-1, 0,000024 ± 0,000009 mg/kg-1 di Sampang. Kandungan seng (Zn) 6,983 ± 0,278 mg kg-1 di Tuban, 5,873 ± 0,233 mg kg-1 di Gresik, 5,172 ± 0,233 mg kg-1 di Sampang.Kata kunci: logam non esensial, logam esensial, sedimenNON-ESSENTIAL METAL CONTENT (PB, CD AND HG) AND ESSENTIAL METALS (CU, CR AND ZN) IN SEDIMENTS IN THE WATERS OF TUBAN GRESIK AND SAMPANG, EAST JAVAABSTRACTHeavy metals such as Pb, Cd, Hg, Cu, Cr and Zn in the water will drop then settle to form sediments, some organisms that feed on the basis of water will have a great chance of being exposed to this heavy metal. This study aims to determine the content of non-essential heavy metals Pb, Cd, Hg and essential heavy metals Cu, Cr, Zn in sediments in the waters of Tuban, Gresik and Sampang. The heavy metal content in this sediment uses Atomic Absorption Spectrometry (AAS). The results showed non-essential metal content of lead (Pb) was 3.3687 ± 0.5257 mg / kg-1 in Gresik, 2.9443 ± 0.3608 mg / kg-1 in Tuban and 3.4253 ± 0.3697 mg / kg-1 in Sampang. The content of Cadmium (Cd) is 2.978 ± 0.224 mg kg-1 in Tuban, 2.955 ± 0.179 mg kg-1 in Gresik, 3.012 ± 0.148 mg kg-1 in Sampang. The content of mercury (Hg) is 0.000029 ± 0.000012 mg kg-1 in Tuban, 0.000033 ± 0.000012 mg kg-1 in Gresik, 0,000034 ± 0,000045 mg kg-1 in Sampang. The results of the analysis of the content of essential metals. Copper (Cu) content 0.0780 ± 0.0069 mg / kg-1 in Tuban, 0.0740 ± 0.0118 mg / kg-1 in Gresik and 0.0750 ± 0.0069 mg / kg-1 in Sampang. Chromium content (Cr) 0,000033 ± 0,0000045 mg / kg-1 in Tuban, 0,000034 ± 0,0000011 mg / kg-1, 0,000024 ± 0,000009 mg / kg-1 in Sampang. The zinc content (Zn) is 6.983 ± 0.278 mg kg-1 in Tuban, 5.873 ± 0.233 mg kg-1 in Gresik, 5.172 ± 0.233 mg kg-1 in Sampang.Keywords: non-essential metals, essential metals, sediments
DIVERSITY OF THE UNIONID FRESHWATER MUSSELS (BIVALVIA: UNIONIDAE) IN BRANTAS RIVER, EAST JAVA, INDONESIA Moch. Affandi; Leonard Ady Candra; Astra Budi Priatama; Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (831.966 KB) | DOI: 10.23869/145

Abstract

The aims of this study are to provide data and information about diversity, dominance, and distribution of the Unionidae mussels species in Brantas River, East Java, Indonesia. Mussel samples were collected from 14 sampling stations along the Brantas River, starting from upstream in Tulungagung to downstream in Surabaya (± 170 km) in April-May 2012. The results showed that five species of mussels, namely: Contradens contradens, Elongaria orientalis, Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis were observed in the Brantas River. Contradens contradens was the “dominant” species, followed by Elongaria orientalis as a “common” species, and Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis as “rare” species. Distribution of each species in the Brantas River tends to be concentrated in downstream of the river, mainly located in station 13. According to the IUCN Red List, Rectidens sumatrensis was categorized in “Data Deficient”, while Pseudodon vondembuschianus and Pilsbryoconcha exilis were categorized in “Least Concern”.
STUDY ON THE HABITAT PREFERENCE OF Diadema setosum IN BAMA COAST BALURAN NATIONAL PARK Clara Puspita; Noer Moehammadi; Bambang Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.021 KB) | DOI: 10.23869/150

Abstract

Diadema setosum is an ecologically important sea urchin which is exists on Bama Coast, but there is no report about the presence of D. setosum in Bama Coast yet. This study was done in order to know the habitat type occurs in Bama Coast, the presence status of D. setosum, distribution pattern, the habitat preference and the character of habitat which is preferred by D. setosum in Bama Coast. Quadrate transect method was used to collect the data. Eight line transect was positioned parallel to the shore line. The transect line was 160 m in length and placed 30 m distance between transects. Quadrate plots of 1 × 1 m2 placed 4 m distance from each plotalong the transect. The habitat type was defi ned by estimated the dominant benthic cover of each plot. Measurement of physical and chemical factor of water includes sea water temperature and salinity. The Distribution pattern was determined by Poisson distribution and Chi-square simultaneously, then continued using Green coeffi cient, the habitat preference was analyzed by Chi-square and the character of habitat which is preferred by D. setosum was analyzed by selection index. The result of this study shows that there are fi ve habitat types in Bama Coast, which are sand, rubble, seagrass, live coral and dead coral. The distribution pattern of D. setosumin Bama Coast is clumped and only presence in three habitat types, which are seagrass, live coral and dead coral. Dadema setosum prefers several habitat type, which are dead coral and live coral and the character of habitat which is preferred by D. setosum is hard and solid substratum with simple architecturally structure.
COMMUNITY STRUCTURE OF WATERBIRDS IN SEVERAL TYPE OF WETLAND UTILIZATION IN EAST COAST OF SURABAYA Ragil Siti Rihadini; Thin Soedarti; Bambang Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.399 KB) | DOI: 10.23869/151

Abstract

Natural wetlands are the natural habitat of waterbirds, but it is very diffi cult to fi nd an unspoiled wetlands without human interventioncurently. East coast of Surabaya is wetlands area in Surabaya that most of the region have change into fi shpond and residental. Theaims of this reasearch are to determine the community structure of waterbirds in each type of wetland utilization and to determine ifthe different type of wetland utilization infl uence the community structure of waterbirds in East Coast of Surabaya. Data were collectedduring August 2012 to January 2013 with point count method. Six sampling plots have been selected. Six sampling plots representingfour types of use of wetlands, which are bozem, the former fi shpond, fi shpond with vegetation, and fi shponds without vegetation (fi shpond1, fi shpond 2 and fi shpond 3). In bozem recorded 19 species of waterbirds (1107 individual), dominated by shorebirds, with a diversityindex (H') 1.86, and evenness index (J') 0.63. In former fi shpond recorded 7 species of water birds (168 individual), dominated by railsand moorhen, with H’ = 1.12 and J’ = 0.57. In fi shpond with vegetation recorded 12 species of water birds (137 individual), dominatedby large wader, with H’ = 1.85 and J’ = 0.74. In fi shpond 1 recorded 18 species of water birds (299 individual), dominated by terns,with H’ = 1.96 and J’ = 0.68. In fi shpond 2 recorded 9 species of water birds (70 individual), dominated by terns, with H’ = 1.73 andJ’ = 0.79. In fi shpond 3 recorded 10 species of water birds (83 individual), dominated by terns, with H’ = 1.84 and J’ = 0.80. Chi-squaretest showed that X2 count is greater than X2 table with α < 0.05, which indicates that there is a signifi cant difference of the numberof individuals of each species and the number of individual of each water birds’ group for each type of use of wetlands, so it can beconcluded that different types of use of wetlands affect water bird community structure in the East Coast Surabaya.
KEANEKARAGAMAN JENIS BURUNG PADA BERBAGAI TIPE PEMANFAATAN LAHAN DI KAWASAN MUARA KALI LAMONG, PERBATASAN SURABAYA – GRESIK Hening Swastikaningrum; Sucipto Hariyanto; Bambang Irawan
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.101 KB) | DOI: 10.23869/204

Abstract

The object of this study was to determined the bird species diversity around the estuary region of Kali Lamong located in between Surabaya and Gresik, East Java. Observation was taken three kilometers far, started from Galang Island in Kali Lamong estuary to upstream side of the river. Twelve stations were made along the river as representative type of five land use. Station I and IV represented settlement area. Station II, III, and VIII represented industrial sites, station V and VI represented lawn, and station VII, IX, and X represented aquaculture. Each stations had 20 meter (bulls eye diameter) range or equivalent with 1.256 m2. Every bird in the area was recorded and analyzed by Shannon- Wiener diverity index. The results of this study shown aquaculture area has the highest diversity index (3,19). Followed by lawn (2,52), industrial site (2,39), mangrove forest (2,06), and settlement area (1,67).