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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
R. Heru Prasetyo
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Biochemistry, Genetics & Molecular Biology Engineering Health Professions Medicine & Pharmacology Neuroscience Other

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Utilization of viable bone marrow derivat stem cells through an adaption in low oxigen tension as an attempt to increase cellular transplantation efficacy for spermatogenesis process Erma Safitri; Suzanita Utama; Candra Bumi; Sri Wigati Mardi; . Mulyani; . Helen; . Purwati; R. Heru Prasetyo; Mas’ud Hariadi; Fedik Abdul Rantam
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.411 KB)

Abstract

Cellular transplantation using stem cells provides very promising solutions in the regeneration and repairment of cells that have experienced degeneration where recovery through medical or surgical intervension is impossible. However, the very low viability of transplanted stem cells limits the transplantation efficacy. The aim of this research was to obtain viable bone marrow derived stem cells by an adaptation treatment in a low oxygen tensioned in vitro culture. Low oxygen tension adaptation was adjusted to the niche of the stem cells in vivo. In this study, in vitro culture of stem cells in 1% oxygen was compared to those of the conventional culture in 21 % oxygen.Results showed that under 1% oxygentension cell proliferation was slower with larger or rounded triangle shaped cells, and senescence or dead cells was low. Meanwhile under 21 % oxygen tension cell proliferation was two fold faster with flattened and slender cells, and senescence or dead cells was higher. In conclusion, conventional in vitro culture under 21% oxygen caused cell aging (senescence) and rapid cell death, therefore the transplanted cells were not viable.
PNEUMOCYSTIS PNEUMONIA (PCP) DI PENDERITA HIV DAN AIDS DENGAN KELAINAN PARU R. Heru Prasetyo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 1 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i1.446

Abstract

PCP is one of the most common opportunistic infection in HIV and AIDS patients. A definitive diagnosis of PCP in HIV and AIDS patients in Dr. Soetomo General Hospital Surabaya has not been previously done. A definitive diagnosis have the role in the therapy and to prevent the illness as well. PCP is diagnosed by using a microscope to indentify Pneumocystis jerovecii in the lung fluid or tisuue. The objective of this study was to know how to detect Pneumocystis jerovecii in the sputum samples and to know the determination of the prevalence of PCP in HIV and AIDS patients suffered with pulmonary symptom who were hospitalized in Dr. Soetomo General Hospital Surabaya. This research was carried out by a cross sectional study utilizing waste sputum samples from HIV and AIDS patients. The detection of Pneumocystis jerovecii used Giemsa stain. Six sputum samples among 18 sputum samples (33.33%) HIV and AIDS patients were Pneumocystis jerovecii positive. Based on this findings HIV and AIDS patients with pulmonary symptoms should be suspect having the possibility of PCP as opportunity infection in HIV and AIDS existed, and there for the detection of the Pneumocystis jerovecii in sputum sample must becoming routinelycarried out in the laboratory examination for HIV and AIDS patients which also suffering pulmonary symptom.
CRYPTOSPORIDIOSIS PARU DI HIV DAN AIDS JS. Hutagalung; R. Heru Prasetyo; Erwin Astha Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.386

Abstract

Although the prevalence of intestinal cryptosporidiosis in Indonesian HIV and AIDS patients were high, however the prevalence of pulmonary cryptosporidiosis have not been previously reported. The objective of this study was to know the determination of the pulmonary cryptosporidiosis prevalence in HIV and AIDS patients with pulmonary symptom that was treated in Dr. Soetomo General Hospital Surabaya. The detection of Cryptosporidium in sputum samples used modified versien acid fast stain of Ziehl Neelsen technique. In this study was found that three (3) of the eight (8) sputum samples (37.5%) of ≥55 years old and CD4≤70 HIV and AIDS patients were Cryptosporidium positive. Based on this study the HIV and AIDS patients with pulmonary symptoms should be suspect having the possibility of pulmonary cryptosporidiosis beside suffered tuberculosis.
CRYPTOSPORIDIOSIS PARU DI PENDERITA TBC R. Heru Prasetyo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.375

Abstract

The pulmonary cryptosporidiosis cases had been reported for immunocompromised persons, most all of whom were secunder infected with HIV and AIDS patients. Tuberculosis is a chronic respiratory disease and tending to cause a weakened immune system (immunocompromised). However, pulmonary cryptosporidiosis has not been previously reported as secunder infection in tuberculosis patients. The objective of this study was to know the prevalence of pulmonary cryptosporidiosis determination in tuberculosis patients. This research was carried out by a cross sectional study utilitzing waste sputum samples from tuberculosis patients. The detection of Cryptosporidium oocyst used modified version acid fast stain of Ziehl Neelsen technique. Three sputum samples among 44 sputum samples (6.8%) had Cryptosporidium oocyst positive. These findings suggest that there was a potential for respiratory transmission of cryptosporidiosis. Although the prevalence of pulmonary cryptosporidiosis in tuberculosis patients are low, the researcher suggest that the possibility of pulmonary cryptosporidiosis as a secondary infection in tuberculosis patients existed, and there for a laboratory examination of pulmonary cryptosporidiosis becoming routinely laboratory for tuberculosis patients.
IMUNOKIMIAWI PGE2 USUS ILEUM YANG KEKURANGAN ENERGI PROTEIN R. Heru Prasetyo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1057

Abstract

Diarrhoeal disease mortality which suffered by children less than five years due to protein energy deficiency is increased significantly.The pathological mechanisms are not known in detail yet. The objective of this research was able to explain the intestine motility inprotein energy deficiency of mice Balb/c. Hormonal mechanism that regulates the intestine motility is roled by prostaglandine E2 hormone(PGE2). The observed variable was the immunity component of PGE2. The measurement was performed using immunohistochemistrymethod with monoclonal antibody against PGE2 to the ileum mice tissue. The result of this study showed that protein energy deficiencymice had much reduced PGE2 (reduction from 1492 to 145, p = 0.000 ). In conclusion, the marked decrease of ileum PGE2 will decreaseintestine motility and it may be one cause of the high diarrhoeal mortality due to protein energy deficiency.
Co-Authors . Helen . Mulyani . Purwati Candra Bumi ERMA SAFITRI Erwin Astha Triyono Fedik Abdul Rantam JS. Hutagalung Mas’ud Hariadi Sri Wigati Mardi Suzanita Utama