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All Journal International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Althea Medical Journal The Indonesian Biomedical Journal Jurnal Sistem Kesehatan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Sylvia Rachmayati
Departemen Patologi Klinik Fakultas Kedokteran Universitas Padjadjaran Rumah Sakit Dr. Hasan Sadikin Bandung
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health

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Effect of Cucumis sativus L on Glucose Absorption through Intestinal Mucosal Membrane of Wistar Rat Models Amalia, Fitri; Surialaga, Samsudin; Rachmayati, Sylvia
Althea Medical Journa Vol 1, No 1 (2014)
Publisher : Althea Medical Journa

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Abstract

Background: The Cucumis sativus L has been used in traditional medicine for several conditions, with one of them being diabetes mellitus. The purpose of this experiment is to observe the anti-hyperglycemic effect of Cucumis sativus L on glucose absorption through the intestinal mucosal membrane of Wistar rats.Methods: The research was conducted on September–November 2012 at Biochemistry Laboratory, Faculty of Medicine, Universitas Padjadjaran. Using in situ perfusion method, the rat’s epithelial mucosa in the intestine was bathed in a simple glucose solution in control, and bathed in glucose and Cucumis sativus L infusion in treatment. After admission, a sample was taken from the digestive tract and the glucose level was measured through a spectrophotometer.Result: There is no significant difference in the absorption of control and treatment solution.Conclusion: The Cucumis sativus L does not have any significant effect on the glucose absorption through the intestinal mucosal membrane. Further studies are still needed to reveal the antihyperglycemic mechanism of Cucumis sativus L.[AMJ.2014;1(1):30–4]Keywords: Antihyperglycemic effect, Cucumis sativus L (Cucumber), Diabetes mellitus, Glucose absorption Pengaruh Cucumis sativus L pada Penyerapan Glukosa melalui usus mukosa Membran Tikus WistarLatar Belakang: Buah timun (Cucumis sativus Linn.) telah dipercaya sebagai obat tradisional beberapa penyakit, salah satunya adalah diabetes melitus. Penelitian ini ditujukan untuk mengetahui efek antihiperglikemi buah timun terhadap transpor glukosa melalui sel epitel mukosa usus halus tikus wistar.Metode: Dengan menggunakan metode perfusi in situ, usus halus tikus dialiri larutan glukosa selama 60 menit untuk kontrol dan larutan glukosa yang telah diberi infusa timun selama 60 menit untuk perlakuan. Sampel yang diambil berupa cairan yang dialirkan ke usus pengambilan dilakukan pada menit ke 0, 15, 30, 45, 60, kemudian dilakukan pengukuran kadar glukosa dengan menggunakan spektofotometri. Penelitian ini dilakukan pada September–November 2012 di Laboratorium Biokimia, Fakultas Kedokteran, Universitas PadjadjaranHasil: Tidak terdapat perbedaan bermakna antara penyerapan glukosa pada larutan kontrol dengan larutan perlakuan.Simpulan: Cucumis sativus L tidak memiliki pengaruh yang signifikan terhadap penyerapan glukosa melalui membran mukosa usus. Penelitian lebih lanjut masih diperlukan untuk mengetahui mekanisme anti hyperglycemic Cucumis sativus L.Kata kunci: Cucumis sativus L (Cucumber), diabetes mellitus, efek antihyperglycemic, penyerapan glukosa
Effect of Spondias dulcis Leaves Infusion on Glucose Transport Rate through Rat Intestine Epithelium Cell Membrane Destia, Krisna; Martiana, Anna; Rachmayati, Sylvia
Althea Medical Journal Vol 3, No 4 (2016)
Publisher : Althea Medical Journal

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Background: Nowadays, people consume herbal remedies made from plants which are believed to be able to relieve certain symptoms and restore normal metabolic functions. Golden apple (Spondias dulcis) leaf has the potential to lower blood glucose. The purpose of this experiment was to observe the anti–hyperglycemic effect of S. dulcisleaves infusion on glucose transport rate through intestinal mucosal membrane of Wistar rats.Methods: An experimental study was conducted from October 2013 to November 2014. Nine male Wistar rats were selected aged 12–16 weeks, weighted 250–400 grams, healthy, actively moving, and had never been used in experiments. The rats were anesthetized, cut open and given three kinds of treatments: three kinds of glucose solutions were flowed through the intestinal tract using in situ perfusion method. Samples obtained from the rat’s intestine were measured with a spectrophotometer to obtain glucose rate transported through intestinal membrane from every treatment. The results of condition 1 and 2, and condition 1 and 3 were then compared.Results: The means of glucose transported through rat intestines epithelium cell membrane were 6.43 mg/dl, 9.40 mg/dl and 9.81 mg/dl. Statistic test result showed there was no significant difference on the glucose transported in every treatment.Conclusions: Glucose absorption rate in rat intestine doesnot decrease after they are given S. dulcis leaves infusion. Therefore, this experiment concludes that S. dulcis leaves do not affect the glucose transport rate in rat intestines epithelium cell membrane. [AMJ.2016;3(4):562–5] DOI: 10.15850/amj.v3n4.939
Korelasi Indeks Massa Tubuh dengan Profil Lipid pada Masyarakat di Jatinangor Tahun 2014 Humaera, Zahra; Sukandar, Hadyana; Rachmayati, Sylvia
Jurnal Sistem Kesehatan Vol 3, No 1 (2017): Volume 3 Nomor 1 September 2017
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.744 KB) | DOI: 10.24198/jsk.v3i1.13956

Abstract

Obesitas merupakan masalah epidemik. Setiap tahunnya terjadi peningkatan persentase individu dengan IMT tinggi (overweight atau obese). Beberapa penyakit tidak menular seperti hipertensi, penyakit jantung koroner (PJK) dan stroke mempunyai faktor risiko yang sama yaitu meningkatnya indeks massa tubuh dan meningkatnya konsentrasi profil lipid. Penelitian ini bertujuan untuk mengetahui korelasi antara indeks massa tubuh dengan konsentrasi profil lipid. Penelitian ini menggunakan data sekunder pendekatan studi potong lintang (cross sectional). Jumlah sampel sebanyak 99 orang. Analisis data menggunakan uji korelasi rank Spearman. Karakteristik sampel pada data yang didapatkan menunjukkan sebanyak 55% subjek termasuk dalam golongan yang mempunyai berat badan yang berlebih (overweight dan obese). Rerata Indeks Massa Tubuh dan profil lipid lebih tinggi pada perempuan bila dibandingkan dengan laki-laki, kecuali untuk kadar TG. Koefisien korelasi IMT dengan kolesterol total (rs = 0,244 p = 0,015), dengan HDL-C (rs=-0,222 p= 0,027), dengan LDL-C (rs = 0,223 p = 0,026), dan dengan TG (rs = 0,242 p = 0,016).Terdapat korelasi yang signifikan antara indeks massa tubuh dengan profil lipid.Kata Kunci: HDL-C, IMT, Kolesterol Total, LDL-C, TG
KORELASI KADAR 8-HYDROXY-2-DEOXYGUANOSINE (8-OHDG) SERUM DENGAN DERAJAT DEFISIT NEUROLOGIS PADA STROK ISKEMIK Liza, -; Parwati, Ida; Birawa, Andi Basuki Prima; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 46, No 3 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Peningkatan stres oksidatif, sekresi radikal bebas pada strok iskemik dapat merusak inti sel neuron di otak. Peningkatan kadar 8-hydroxy-2-deoxyguanosine (8-OHdG) serum pada strok iskemik yang merupakan hasil interaksi radikal bebas dengan gugus C8 basa guanin menandakan kerusakan sel neuron otak yang tercermin pada penilaian defisit neurologis menggunakan skor The National Institutes of Health Stroke Scale (NIHSS). Tujuan penelitian ini untuk mengetahui korelasi kadar 8-OHdG serum dengan derajat defisit neurologis menggunakan skor NIHSS. Bentuk penelitian adalah observasional dengan desain penelitian potong lintang. Penelitian dilakukan di RSUP Dr. Hasan Sadikin Bandung pada bulan Agustus 2013 hingga Januari 2014. Subjek penderita stroke iskemik onset akut berdasarkan CT-scan. Analisis statistik menggunakan Uji Kruskal-Wallis, Uji korelasi rank Spearman?s. Sebanyak 72 subjek penelitian diperoleh median kadar 8-OHdG dalam serum dengan defisit neurologis ringan 3,9 ng/mL (3,3?12,0 ng/mL), sedang 23 ng/mL (8,0?51,0 ng/mL), berat 77,5 ng/mL (54,0-97,0 ng/mL). Korelasi kadar 8-OHdG serum dengan derajat defisit neurologis rs=0,912 (p<0,001). Simpulan, kadar 8-OHdG dalam serum pada stroke iskemik berkorelasi positif sangat kuat, dengan derajat defisit neurologis, hal ini dapat dipertimbangkan untuk digunakan pada keadaan ketidaksesuaian antara gambaran CT-scan dan klinis atau pada fasilitas kesehatan yang tidak mempunyai CT scan.Kata kunci: Defisit neurologis, stroke iskemik, 8-OHdG dalam serum Correlation between Serum 8-Hydroxy-2-Deoxyguanosine (8-OHdG) and Neurological Deficits in Ischemic Stroke Increased oxidative stress and free radicals can cause neuron cell damage. Serum 8-hydroxy-2-deoxyguanosine (8-OHdG) concentration is the result of free radical interactions with cluster C8 guanine bases, which is used to assess the degree of neuron cells damage and the oxidative stress levels. Increased serum 8-OHdG concentration indicating brain cells damage is reflected in neurological deficits based on the The National Institutes of Health Stroke Scale (NIHSS). The aim of this study was to determine the correlation between serum  8-OHdG concentration and the degree of neurological deficit by NIHSS. This was an observational cross-sectional study. Seventy-two patients with acute ischemic stroke who visited Dr. Hasan Sadikin General Hospital Bandung during the period of August 2013 to January 2014 were enrolled. Statistical analysis was performed using Kruskal-Wallis test and rank Spearman?s correlation test. The mild neurological deficit median serum 8-OHdG concentrations was 3.9 ng/mL (3.3?12.0 ng/mL), moderate was 23 ng/mL (8.0?51.0 ng/mL), and severe was 77.5 ng/mL (54.0?97.0 ng/mL). Correlation of serum 8-OHdG concentration with neurological deficits in acute ischemic stroke rs=0.912 (p<0.001). In conclusion, serum 8-OHdG have a strong meaningful positive correlation with neurological deficits. Serum 8-OHdG concentration may be considered to be use in the assessment of discrepancy between CT scan and clinical symptoms and in health facilities with no CT scan facility.Key words: Ischemic stroke, neurological deficits, serum 8-OHdG DOI: 10.15395/mkb.v46n3.313
UJI DIAGNOSTIK PEMERIKSAAN KADAR TRANSFORMING GROWTH FACTOR BETA 1 CAIRAN PLEURA PADA PLEURITIS TUBERKULOSIS Wahyuningrum, Woro Hapsari; Indrati, Agnes Rengga; Rachmayati, Sylvia; Turbawaty, Dewi Kartika
Majalah Kedokteran Bandung Vol 49, No 4 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.438 KB) | DOI: 10.15395/mkb.v49n4.1152

Abstract

Sulitnya penegakan diagnosis pleuritis tuberkulosis (TB) disebabkan oleh gambaran klinis yang tidak spesifik dan rendahnya sensitivitas pemeriksaan bakteriologik, akibat cairan pleura yang bersifat pausibasiler.Transforming growth factor beta 1 (TGF-?1) mampu menekan respons imun seluler terhadap M. tuberculosis, berperan dalam aktivasi TB laten, kerusakan jaringan, dan fibrosis. Penelitian ini bertujuan mengetahui peranan pemeriksaankadarTGF-?1 cairan pleura dalam penegakan diagnosis pleuritis TB. Penelitian ini adalah uji diagnostik, pengambilan data secara potong lintang. Bahan pemeriksaan berupa cairan pleura yang disimpan dalam ultra low freezer dari pasien rawat inap di RSUP Dr. Hasan Sadikin (RSHS) Bandung dan Rumah Sakit Paru Dr. H.A Rotinsulu Bandung periode Oktober 2014 ? Maret 2015. Total subjek penelitian 68, terdiri dari 17 pleuritis TB confirmed, 22 pleuritis TB probable, dan 29 pleuritis non TB.Kadar TGF-?1 cairan pleura pleuritis TB: 41,4 (10,4 ?19.481) pg/mL. Pemeriksaan kadar TGF-?1 menunjukkan sensitivitas 66,7%, spesifisitas 65,5% pada nilai cut-off  >37 pg/mL. Sensitivitas pemeriksaan kadar  TGF-?1 lebih tinggi bila dibanding dengan pemeriksaan bakteriologik. Hasil negatif pada pemeriksaan bakteriologik dari subjek pleuritis TB probable, 77,3% di antaranya memberikan hasil positif pada pemeriksaan kadar TGF-?1.  Pemeriksaan ini dapat digunakan sebagai tambahan modalitas pemeriksaan laboratorium untuk mendiagnosis pleuritis TB, terutama bila didapatkan hasil negatif pada pemeriksaan bakteriologik, namun kecurigaan pleuritis TB sangat kuat.Kata kunci: Kadar TGF-?1, pemeriksaan bakteriologik, pleuritis TB  Diagnostic Value of Pleural Fluid Transforming Growth Factor Beta 1 Level in Tuberculous PleurisyTuberculous(TB) pleurisy was difficult to be diagnosed as it has nonspecific symptoms and low sensitivity in  bacteriological tests which are caused by paucibacillary of pleural fluid. Transforming Growth Factor Beta 1 (TGF-?1) could suppress  the immune response to M. tuberculosis infection, activating latent TB infection that involves tissue damages and fibrosis. This study aimed to determine the role of pleural fluid TGF-?1 level in diagnosing TB pleurisy. This was a cross-sectional specific descriptive observational study involving a total subjects of 68 that consisted of  17 confirmed TB pleurisy, 22 probable TB pleurisy, and 29 non-TB pleurisy. Pleural fluid collected in October 2014 ? March 2015 from the subjects was stored in an ultra low freezer. The which.level of TGF-?1 in pleural fluid in TB pleurisy was 41,4(10,4 ? 19.481) pg/mL. Pleural  fluid TGF-?1 level showed a sensitivity of 66,7%, specificity of 65,5% and a cut-off value >37pg/mL. The sensitivity level of pleural fluid TGF-?1 was higher than that of the bacteriological test. Pleural fluid TGF-?1 level can be used as an adjunct modality of laboratory test for diagnosing TB pleurisy, especially for patient with high suspiscion of TB pleurisy but yield negative result in the bacteriological test.Key words: Bacteriological tests, TB pleurisy, TGF-?1 levels
KORELASI JUMLAH FOLIKEL ANTRAL DENGAN KADAR 25(OH)D SERUM PADA PENDERITA SINDROM OVARIUM POLIKISTIK Mustari, Asri Dini; Rostini, Tiene; Indrati, Agnes Rengga; Bayuaji, Hartanto; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 50, No 4 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.066 KB) | DOI: 10.15395/mkb.v50n4.1469

Abstract

Sindrom ovarium polikistik (SOPK) ditandai oleh hiperandrogenisme, oligo atau anovulasi, dan morfologi ovarium polikistik. Penanda ovarium polikistik, yaitu terdapat ?12 folikel antral dengan diameter 2?9 mm pada pemeriksaan ultrasonografi. Vitamin D (25-hidroksivitamin D; 25(OH)D) berperan pada proses steroidogenesis di sel teka ovarium dan pengaturan ekspresi reseptor follicle stimulating hormone (FSH) di sel granulosa ovarium. Defisiensi 25(OH)D menyebabkan penurunan aktivitas FSH, sehingga folikel antral di ovarium berukuran kecil dan berjumlah banyak. Tujuan penelitian ini mengetahui korelasi jumlah folikel antral dengan kadar 25(OH)D serum pada penderita SOPK. Uji observasional analitik  dengan rancangan potong lintang di Rumah Sakit Umum Pusat Dr. Hasan Sadikin (RSHS) Bandung dilakukan selama bulan September 2017?Juni 2018. Subjek penelitian adalah 52 penderita SOPK yang telah didiagnosis oleh klinisi obstetri dan ginekologi RSHS Bandung. Pengambilan darah vena dilakukan untuk pemeriksaan kadar 25(OH)D serum dengan metode ELISA. Hasil penelitian ini mendapatkan 56% subjek memiliki kadar 25(OH)D serum ?20 ng/mL. Hasil uji korelasi Spearman didapatkan korelasi negatif kuat yang bermakna (r = -0,867, p<0,001) jumlah folikel antral dengan kadar 25(OH)D serum. Kadar 25(OH)D serum yang rendah pada penderita SOPK berkorelasi dengan jumlah folikel antral yang banyak. Terdapat korelasi negatif antara jumlah folikel antral dan kadar 25(OH)D serum pada penderita SOPK.  Kata kunci: Folikel antral, 25(OH)D serum, sindrom ovarium polikistik  Correlation between Antral Follicles Count and Serum 25(OH)D Levels in Polycystic Ovary Syndrome PatientsPolycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, oligo- or anovulation, and polycystic ovarian morphology. Vitamin D (25-hydroxyvitamin D; 25(OH)D) plays a role in the steroidogenesis process in the ovarian theca cells and regulates follicle stimulating hormone (FSH) receptor expression in granulosa cells. In 25(OH)D deficiency there is a decrease in FSH activity that the follicles become small and the antral follicles count in the ovary increases. The aim of this study was to determine the relationship between antral follicles count and serum 25(OH)D levels in PCOS patients. A cross-sectional analytical observational study was conducted in Dr. Hasan Sadikin General Hospital Bandung in September 2017?June 2018. Subjects of this study were 52 PCOS patients diagnosed by obstetricians and gynecologists of the hospital. Blood vein samples were collected to examine serum 25(OH)D levels using ELISA method. Serum 25(OH)D level of ?20 ng/mL was found in 55.8% of the subjects in this study. Analysis using Spearman?s correlation test showed a significant negative correlation (r = -0.867, p <0.001) between antral follicles count and serum 25(OH)D level in patients with PCOS. Lower levels of 25(OH)D serum in PCOS patients correlates with higher number of antral follicles count. There is a negative correlation between antral follicles count and serum 25(OH) D level in PCOS patients.Key words: Antral follicle count, 25(OH)D serum levels, polycystic ovary syndrome 
Perbedaan Kadar Osteoprotegerin Serum Penderita Diabetes Melitus Tipe 2 tanpa dan dengan Penyakit Jantung Koroner Nestiti, Ratri Dwitiya; Noormartany, -; Dewi, Nina Susana; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Diabetes melitus (DM) tipe 2 merupakan diabetes yang disebabkan oleh resistensi insulin dan atau gangguan sekresi insulin sehingga menyebabkan hiperglikemia yang merupakan penyebab disfungsi endotel. Disfungsi endotel merupakan proses awal aterosklerosis dan merupakan salah satu faktor risiko yang menyebabkan penyakit jantung koroner (PJK). Sampai saat ini angiografi koroner digunakan sebagai baku emas PJK. Pemeriksaan osteoprotegerin (OPG) merupakan pemeriksaan alternatif sebagai penanda disfungsi endotel, kurang invasif, lebih dini, dan murah. Osteoprotegerin berhubungan dengan perkembangan plak arteri pada penderita DM tipe 2. Tujuan penelitian ini mengetahui perbedaan kadar OPG dalam serum penderita DM tipe 2 tanpa dan dengan PJK. Penelitian dilakukan pada September–Desember 2012 di Poliklinik Endokrin dan Instalasi Gawat Darurat RSUP Dr. Hasan Sadikin Bandung. Subjek penelitian sebanyak 102 orang, 51 orang penderita telah didiagnosis oleh klinisi sebagai penderita DM tipe 2 dengan PJK dan 51 orang penderita DM tipe 2 tanpa PJK, berusia 35–75 tahun. Pemeriksaan OPG menggunakan metode enzyme linked immunosorbent assay (ELISA). Bentuk penelitian adalah observasional komparatif dengan rancangan penelitian studi perbandingan potong lintang. Analisis statistik menggunakan uji Shapiro Wilk’s, independent T, dan Mann Whitney. Nilai median kadar OPG serum untuk penderita DM tipe 2 dengan PJK lebih tinggi, yaitu sebesar 6,1 pmol/L dan penderita DM tipe 2 tanpa PJK, yaitu sebesar 2,6 pmol/L (p=0.0001). Simpulan, terdapat perbedaan kadar OPG serum antara kelompok DM tipe 2 tanpa dan dengan PJK. [MKB. 2015;47(4):218–23]Kata kunci: Disfungsi endotel, DM tipe 2, osteoprotegerinDifferences of Osteoprotegerin Levels in Patients with Type 2 Diabetes Mellitus with and without Coronary Arterial DiseaseAbstractType 2 diabetes mellitus (DM) is caused by insulin resistance and/or impaired insulin secretion, thus causing hyperglycemia leading to endothelial dysfunction. Endothelial dysfunction is an early process of atherosclerosis and is one of the risk factors for coronary arterial disease (CAD). Currently, coronary angiography is used as the gold standard for diagnosing CAD. Examination of osteoprotegerin (OPG) is an alternative assay for endothelial dysfunction marker which is less invasive, has the ability to detect earlier, and less expensive. Osteoprotegerin is associated with the development of arterial plaque in patients with type 2 DM. The aim of this study was to determine the differences in serum OPG levels in patients with type 2 DM with and without CAD. The study was conducted in September–December 2012 in the Endocrine Clinic and Emergency Unit of Dr. Hasan Sadikin General Hospital Bandung on 102 patients aged between 35–75 years, with 51 subjects type 2 DM patients with CAD and 51 subjects with type 2 DM without CAD, both diagnosed by clinician. The method used was, OPG enzyme linked immunosorbent assay (ELISA) examination method. The study was an observational cross-sectional comparative study. Statistical analysis was performed using normality test with Shapiro Wilk’s, independent T and Mann Whitney test. The median value for patients with type 2 DM with CAD equaled to 6.1 pmol/L and the value for type 2 diabetic patients without CAD was 2.6 pmol/L. In this study, the OPG levels obtained in patients type 2 DM with CAD were higher than those without CAD. In conclusion, there is a significant difference in OPG levels between type 2 DM with and without CAD groups. [MKB. 2015;47(4):218–23]Key words: Endothelial dysfunction, type 2 diabetes, osteoprotegerin DOI: 10.15395/mkb.v47n4.616
Perbedaan Kadar Liver Fatty Acid Binding Protein (L-FABP) Urine Penderita Diabetes Melitus Tipe 2 dengan Normoalbuminuria dan Mikroalbuminuria Wiharjo, Kristina; Permana, Hikmat; Noormartany, -; Rachmayati, Sylvia
Majalah Kedokteran Bandung Vol 46, No 2 (2014)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Diabetes melitus (DM) merupakan salah satu penyebab end stage renal disease (ESRD), 20−40% akan mengalami nefropati diabetik yang berkembang menjadi ESRD. Prevalensi nefropati diabetik meningkat pada DM tipe 2. Akumulasi stres oksidatif dapat menyebabkan peningkatan permeabilitas glomerulus sehingga menyebabkan peningkatan ekskresi albumin pada urine yang terbagi menjadi normoalbuminuria, mikroalbuminuria, dan makroalbuminuria. Kerusakan glomerulus terjadi setelah kerusakan tubulointerstisial ginjal yang menyebabkan penurunan aliran kapiler peritubular dan menyebabkan hipoksia. Liver fatty acid binding protein (L-FABP) berfungsi mengurangi hipoksia dengan mengikat stres oksidatif dan mengeluarkannya ke dalam urine. Penelitian ini bertujuan untuk menganalisis apakah terdapat perbedaan kadar L-FABP antara penderita DM tipe 2 normoalbuminuria dan mikroalbuminuria. Metode penelitian adalah observasional analitik dengan rancangan potong lintang. Subjek penelitian sebanyak 70 orang penderita DM tipe 2 dengan normoalbuminuria (38 orang) dan mikroalbuminuria (32 orang) yang diambil secara acak pada pasien yang datang ke Laboratorium Rawat Jalan Rumah Sakit Dr. Hasan Sadikin pada periode Juli−September 2012. Uji statistik menggunakan Uji Mann-Whitney. Hasil penelitian menunjukkan perbedaan bermakna kadar L-FABP urine pada normoalbuminuria dengan mikroalbuminuria dengan nilai ZM-W=3.513; p<0,001, median pada normoalbuminuria adalah 5, dan mikroalbuminuria adalah 7. Dari penelitian ini didapatkan kadar L-FABP urine meningkat karena kompensasi pembentukan stres oksidatif dan hipoksia yang terjadi sebelum kerusakan glomerulus. Simpulan, kadar L-FABP urine pada pasien DM tipe 2 dengan mikroalbuminuria lebih tinggi daripada pasien DM tipe 2 dengan normoalbuminuria. [MKB. 2014;46(2):83–7]Kata kunci: Diabetes melitus tipe 2, kadar L-FABP urine, mikroalbuminuria, nefropati diabetik, normoalbuminuria Comparison of Urinary Liver-Fatty Acid Binding Protein (L-FABP) in Normoalbuminuria and Microalbuminuria in Type 2 Diabetes Mellitus PatientsDiabetes mellitus (DM) is the leading cause of the end stage renal disease (ESRD). Around 20−40% patients with DM develop diabetic nephropathy and eventually progress into ESRD. Type 2 DM has a greater prevalence to develop diabetic nephropathy. Oxidative stress accumulation can increase permeability of the glomerulus which results in increased urine albumin excretion, which is divided into three groups: normoalbuminuria, microalbuminuria and macroalbuminuria. Glomerulus dysfunction occurs after tubulointerstisial renal dysfunction which decreases peritubular capillary flow that leads to tubulointerstisial hypoxia. Liver fatty acid binding protein function is to reduce hypoxia by binding oxidative stress and excretes it into urine. The aim of this study was to analyze the differences in the urine L-FABP level between normoalbuminuria and microalbuminuria type 2 DM patients. The study design was observational analytic using cross-sectional method. Subjects were 70 DM type 2 patients with normoalbuminuria (38 patients) and microalbuminuria (32 patients). Statistical analysis was performed using the Mann Whitney test The results found that there were significant differences in levels of urine L-FABP between normoalbuminuria and microalbuminuria type 2 DM patients (ZM-W=3.513, p<0.001) with medians of 5 and 7 in normoalbuminuria and microalbuminuria, respectively. The urine L-FABP level increased because of the oxidative stress and hypoxia that happened before the glomerulus dysfunction. In conclusion, urine L-FABP level in patients DM type 2 with microalbuminuria is higher than that of the normoalbuminuria. [MKB. 2014;46(2):83–7]Key words: diabetic nephropathy, L-FABP urine level, microalbuminuria, normoalbuminuria, type 2 DM DOI: 10.15395/mkb.v46n2.278
Effect of Spondias dulcis Leaves Infusion on Glucose Transport Rate through Rat Intestine Epithelium Cell Membrane Krisna Destia; Anna Martiana; Sylvia Rachmayati
Althea Medical Journal Vol 3, No 4 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Abstract

Background: Nowadays, people consume herbal remedies made from plants which are believed to be able to relieve certain symptoms and restore normal metabolic functions. Golden apple (Spondias dulcis) leaf has the potential to lower blood glucose. The purpose of this experiment was to observe the anti–hyperglycemic effect of S. dulcisleaves infusion on glucose transport rate through intestinal mucosal membrane of Wistar rats.Methods: An experimental study was conducted from October 2013 to November 2014. Nine male Wistar rats were selected aged 12–16 weeks, weighted 250–400 grams, healthy, actively moving, and had never been used in experiments. The rats were anesthetized, cut open and given three kinds of treatments: three kinds of glucose solutions were flowed through the intestinal tract using in situ perfusion method. Samples obtained from the rat’s intestine were measured with a spectrophotometer to obtain glucose rate transported through intestinal membrane from every treatment. The results of condition 1 and 2, and condition 1 and 3 were then compared.Results: The means of glucose transported through rat intestines epithelium cell membrane were 6.43 mg/dl, 9.40 mg/dl and 9.81 mg/dl. Statistic test result showed there was no significant difference on the glucose transported in every treatment.Conclusions: Glucose absorption rate in rat intestine doesnot decrease after they are given S. dulcis leaves infusion. Therefore, this experiment concludes that S. dulcis leaves do not affect the glucose transport rate in rat intestines epithelium cell membrane. [AMJ.2016;3(4):562–5] DOI: 10.15850/amj.v3n4.939
Effect of Cucumis sativus L on Glucose Absorption through Intestinal Mucosal Membrane of Wistar Rat Models Fitri Amalia; Samsudin Surialaga; Sylvia Rachmayati
Althea Medical Journal Vol 1, No 1 (2014)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.91 KB)

Abstract

Background: The Cucumis sativus L has been used in traditional medicine for several conditions, with one of them being diabetes mellitus. The purpose of this experiment is to observe the anti-hyperglycemic effect of Cucumis sativus L on glucose absorption through the intestinal mucosal membrane of Wistar rats.Methods: The research was conducted on September–November 2012 at Biochemistry Laboratory, Faculty of Medicine, Universitas Padjadjaran. Using in situ perfusion method, the rat’s epithelial mucosa in the intestine was bathed in a simple glucose solution in control, and bathed in glucose and Cucumis sativus L infusion in treatment. After admission, a sample was taken from the digestive tract and the glucose level was measured through a spectrophotometer.Result: There is no significant difference in the absorption of control and treatment solution.Conclusion: The Cucumis sativus L does not have any significant effect on the glucose absorption through the intestinal mucosal membrane. Further studies are still needed to reveal the antihyperglycemic mechanism of Cucumis sativus L.[AMJ.2014;1(1):30–4]Keywords: Antihyperglycemic effect, Cucumis sativus L (Cucumber), Diabetes mellitus, Glucose absorption Pengaruh Cucumis sativus L pada Penyerapan Glukosa melalui usus mukosa Membran Tikus WistarLatar Belakang: Buah timun (Cucumis sativus Linn.) telah dipercaya sebagai obat tradisional beberapa penyakit, salah satunya adalah diabetes melitus. Penelitian ini ditujukan untuk mengetahui efek antihiperglikemi buah timun terhadap transpor glukosa melalui sel epitel mukosa usus halus tikus wistar.Metode: Dengan menggunakan metode perfusi in situ, usus halus tikus dialiri larutan glukosa selama 60 menit untuk kontrol dan larutan glukosa yang telah diberi infusa timun selama 60 menit untuk perlakuan. Sampel yang diambil berupa cairan yang dialirkan ke usus pengambilan dilakukan pada menit ke 0, 15, 30, 45, 60, kemudian dilakukan pengukuran kadar glukosa dengan menggunakan spektofotometri. Penelitian ini dilakukan pada September–November 2012 di Laboratorium Biokimia, Fakultas Kedokteran, Universitas PadjadjaranHasil: Tidak terdapat perbedaan bermakna antara penyerapan glukosa pada larutan kontrol dengan larutan perlakuan.Simpulan: Cucumis sativus L tidak memiliki pengaruh yang signifikan terhadap penyerapan glukosa melalui membran mukosa usus. Penelitian lebih lanjut masih diperlukan untuk mengetahui mekanisme anti hyperglycemic Cucumis sativus L.Kata kunci: Cucumis sativus L (Cucumber), diabetes mellitus, efek antihyperglycemic, penyerapan glukosa DOI: 10.15850/amj.v1n1.294
Co-Authors - Liza Abdul Hadi Martakusumah Agnes Rengga Indrati Andi Basuki Prima Birawa Anita Liliana Susanti Anna Martiana Anna Martiana Anna Tjandrawati Basti Andriyoko Budi Setiabudiawan Destia, Krisna Dewi Kartika Turbawaty Erna Jupri Farisa Raudina Fitri Amalia Fitri Amalia Hadyana Sukandar Hadyana Sukandar Hartanto Bayuaji Hikmat Permana Humaera, Zahra Ida Parwati Jusuf Sulaeman Effendi Krisna Destia Kristina Wiharjo Leni Lismayanti Mustari, Asri Dini Nanan Sekarwana Nida Suraya Nina Susana Dewi Noormartany . Rachmat Soelaeman Raden Tina Dewi Judistiani Ratri Dwitiya Nestiti, Ratri Dwitiya Rita Rachmayanti Ryan Bayusantika Ristandi Samsudin Surialaga Setyorini Irianti Tiene Rostini Tiene Rostini, Tiene Wahyuningrum, Woro Hapsari Yudi Mulyana Hidayat Zahra Humaera