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All Journal Agrica: Journal of Sustainable Dryland Agriculture
Dionisius Tungga
Fakultas Pertanian Universitas Flores
Agriculture, Biological Sciences & Forestry Chemistry

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ISOLASI DAN IDENTIFIKASI SENYAWA YANG BERSIFAT ANTIBAKTERI DAN TOKSIK DALAM TUMBUHAN ILER {Coleiis scutellarioides [L.] Benth) Dionisius Tungga; I Made Dira Swantara; Yenni Ciawi
AGRICA Vol. 1 No. 2 (2008): December
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v1i2.484

Abstract

The isolation and identification of antibacterial and toxic compound on Coleiis scutellarioides [L.] Benth. has been conducted. The extraction applied was maceration method using ethanol 85 %. The ethanol extract then partitioned using three kinds of solvents namely n-hexane, chloroform, ethyl acetate respectively. The most toxic and active antibacterial extraction was separated by chromatography column with n-hexane: chloroform as the mobile phase. Identification of the active isolate was conducted by gas chromatography-mass spectroscopy with the aim of'Wiley 229.L database. Toxic test and antibacterial activity test showed that FB2 was proved to be the most toxic and active antibacterial one. The most toxic and active antibacterial fraction of FB2 showed a retardation area of 66,98 mm2 to Micrococcus luteus and 54,43 mm2 to Escherichia coli, and LC50 at 90,72 ppm. Gas chromatography-mass spectroscopy data show that the FB2 fraction contains 4 compounds namely palmitate acid (24,41%); stearate acid (19,53%); 9-oktadekenamida (22,95%); and Esther dioctyl hexadioat (33,11 %»)•
PRODUKSI KARBONDIOKSIDA (CO,) SEBAGAI INDIKATOR RESPIRASI PADA BERBAGAI SAYURAN Josina Irene Brigetha Hutubessy; Dionisius Tungga
AGRICA Vol. 2 No. 1 (2009): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v2i1.546

Abstract

The objective of the research was to determine the respiration rate of vegetable through calculating CO, content resulted from the respiration process. The nature of the research was experimental research which held in a laboratory at the Faculty of Agriculture, Flores University. The result showed that respiration process released C02 and energy. Titration result in yam showed higher C02 content in comparison to cabbage and mustard green. However, the respiration rate of mustard green was higher than other vegetables. The logic behind the results was that the position and the number of stomata per area of the leaf determine respiration rate. In this case, the number of stomata on the mustard green was higher than other observed vegetables. In fact, the respiration rate of vegetables is determined by several factors. Specifically, some internal and external factors affect it, such as soluble matters availability, oxygen, temperature, type and development stage of plants. Morphologically, certain vegetables produce more moisture content than others, which is, in turn, produce low respiration rate.
Co-Authors Hutubessy, Josina Irene Brigetha I Made Dira Swantara Jamhari Jamhari