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All Journal Jurnal Informatika Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Pharmaciana: Jurnal Kefarmasian Prosiding Forum Studi Transportasi Antar Perguruan Tinggi International Journal of Natural Science and Engineering PUBLIKASI PENELITIAN TERAPAN DAN KEBIJAKAN
Budi Raharjo
Sekolah Teknik Elektro dan Informatika, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Mathematics Medicine & Pharmacology Public Health Social Sciences Transportation Other

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IDENTIFIKASI POTENSI INTERAKSI OBAT PADA PASIEN RAWAT INAP PENYAKIT DALAM DI RSUD Prof. Dr. MARGONO SOEKARJO PURWOKERTO DENGAN METODE OBSERVASIONAL RETROSPEKTIF PERIODE NOVEMBER 2009 - JANUARI 2010 Sari, Andriana; Wahyono, Djoko; Raharjo, Budi
PHARMACIANA Vol 2, No 2: November 2012
Publisher : PHARMACIANA

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Abstract

Potensi interaksi obat adalah potensi aksi suatu obat diubah atau dipengaruhi oleh obat lain yang diberikan bersamaan. Interaksi obat didefinisikan sebagai fenomena yang terjadi ketika efek farmakodinamik dan farmakokinetik dari suatu obat berubah karena adanya pemberian obat yang lain. Interaksi obat dapat menyebabkan advers drug reaction apabila potensi terjadinya interaksi tersebut tidak diketahui sebelumnya upaya optimalisasi tidak dapat dilakukan. Tujuan penelitian ini adalah mengidentifikasi potensi interaksi obat pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto. Metode penelitian adalah observasional retrospektif (November 2009 - Januari 2010) dengan menggunakan metoda deskriptif untuk analisa data. Hasil penelitian menunjukkan potensi interaksi obat pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto sebesar 56,76% (n = 259). Berdasarkan kategori signifikansi yang dikemukakan Tatro (2006), terdapat potensi interaksi obat kategori signifikansi 1 pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto sebesar 16,60%. Penggunaan obat berpotensi interaksi yang masuk kategori signifikansi 1 sebaiknya disesuaikan dengan kebutuhan pasien akan obat, risk and benefit serta dilakukan upaya optimalisasi.
MODA BUS KE MODA KERETA API JURUSAN SURABAYA – MOJOKERTO Widyastuti, Hera; Buana, Cahya; Istiar, Istiar; Herijanto, Wahju; Kartika, Anak Agung Gde; Arif, Catur; Raharjo, Budi; Solikhah, Ummatus; Prabawati, Aldila Riana
Prosiding Forum Studi Transportasi Antar Perguruan Tinggi Vol 3 No 1 (2015): The 17th FSTPT of International Symposium
Publisher : FSTPT Indonesia

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Abstract

Under the terms of the capacity, the railway mode is more efficient in terms of moving passengers and goods as to accommodate more passengers and goods than bus modes. Moreover, when there are obstacles in the road such as road maintenance progress, and hole on the road, flooding, existence of demonstration and others, bus modes will not be able to serve passenger well. However in terms of mobility and flesibility the bus is more flexible than the train because it hasn’t limited by the schedule The government started to build new tracks to reduce the delay in the arrival of trains and increase the frequency of train departures. The new tracks are expected to make better services for passengers and goods. Under these conditions, they need to do research on the possibility displacement modes of transportation on passengers. Stated Preference technique used to obtain data on the willingness of passengers to switch modes by distributing questionnaires to Surabaya-Mojokerto line passengers. Those questionnaire are distributed in three terminals which are Joyoboyo Terminal (Surabaya), Purabaya Terminal, Bungurasih (Sidoarjo), and Kertajaya Terminal (Mojokerto). The obtained data were analyzed using Binary Logistic Regression. The result shown that respondent who spent 45 minute willing to move from bus to train is 27,13% whilst 60 minute is 48,10%. Considering the money spending of the respondent each time travel using bus in which stated as Rp. 15.000 it’s only 12,02% who are willing to move from bus to train.
APLIKASI WEB GRABBER UNTUK MENGAMBIL HALAMAN WEB SESUAI DENGAN KEYWORD YANG DIINPUTKAN Budhi, Gregorius Satia; Setiabudi, Djoni Haryadi; Raharjo, Budi
Jurnal Informatika Vol 7, No 1 (2006): MAY 2006
Publisher : Institute of Research and Community Outreach - Petra Christian University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.919 KB) | DOI: 10.9744/informatika.7.1.pp. 24-29

Abstract

At this time the use of search engine on the Internet still does not reach maximum result. Users need to browse every web page list as the result of the search that fit with the topic one by one. We need an application that has a capability to look and to save web pages with their links fit with the topic without browsing, so that the result can be accessed offline. This application is called Web Grabber. Web Grabber application uses inputs such as keyword and searching depth. The input will be processed to obtain the appropriate web pages with the keyword and the searching depth, which is automatically saved. Firstly a keyword is needed to be search in the available search engine to get the search lists, which can be picked by the user. Every pick list will be used as the first search page. This application results are web pages that contain the keyword. This application also has a facility to browse the results. This application was succeed to automatically save web pages that appropriate with the input keyword and the depth, that previously search in AltaVista or Google. Web pages that contain with the keyword saved in the computer so that it can be browsed offline. Abstract in Bahasa Indonesia : Saat ini penggunaan search engine pada internet masih belum memperoleh hasil yang maksimal. Pengguna masih harus browsing halaman-halaman web hasil pencarian untuk mencari topik yang diinginkan satu-persatu. Untuk itu perlu dibuatkan aplikasi yang dapat mencari dan menyimpan halaman-halaman web dengan link-link-nya sesuai topik tanpa harus melalui proses browsing, sehingga hasilnya dapat dilihat secara offline. Aplikasi ini biasa disebut Web Grabber. Program aplikasi Web Grabber ini mendapatkan inputan berupa keyword dan kedalaman pencarian. Inputan ini akan diproses untuk memperoleh hasil berupa halaman - halaman web yang disimpan secara otomatis sesuai dengan keyword yang diberikan dan tingkat kedalaman pencariannya. Proses didahului dengan pencarian keyword pada search engine yang tersedia menjadi sebuah list yang dapat dipilih oleh pengguna. Hasil aplikasi ini berupa halaman - halaman web yang mengandung keyword. Aplikasi juga menyediakan fasilitas untuk browsing hasil yang didapat. Program yang dibuat berhasil melakukan penyimpanan halaman-halaman web sesuai dengan keyword yang diberikan dengan tingkat kedalaman tertentu, yang sebelumnya dicari melalui search engine Altavista atau Google. Halaman-halaman web yang mengandung keyword dapat diambil dan disimpan pada komputer sehingga dapat di-browse secara offline. Kata kunci: web grabber, keyword, kedalaman, search engine, internet.
Isolasi Dan Uji Aktivitas Kitinase Isolat Bakteri Dari Kawasan Geotermal Dieng Nafisah, Hidayatun; Pujiyanto, Sri; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.301 KB) | DOI: 10.14710/bioma.19.1.22-29

Abstract

Chitinase (EC.3.2.2.14) is an enzyme which can degradatechitin became N-acetilglucosamin. Chitinase has many benefits made the demand of it increases. High demands spur its availability in large quantities, cheap, fast production, resistant to any physical factor and chemical environment. Rapid and resistant enzyme production to environment factor can be obtained using chitinolitic bacteria of Geothermal Dieng. The utilization of chitin as bacterial growth substrate from waste of shell crab can be done considering high prices of commercial chitin on the market. The purpose of the research is to get the isolate of termoleranchitinolitic of watery mud in Geothermal Dieng and to know the character of the chosen isolate producing highest chinitase activity type of chitin source treatment and pH of media production. The research is done by growing the chitinolitic in the room temperature for 14 days. The experimental design used in this study is a complete randomized design of factorial pattern (two factors). The first factor is the type of chitin source that includes commercial chitin and chitin crab kits. The second factor is the pH of liquid chitin media for the production of enzymes, ie pH 6, 7 and 8.Chitinase activity is tested by measuring the result of sugar reduction. Obtained data is analyzed with Analysis of Variance (ANOVA). Result of isolation and selection is obtained one potential isolate, KSR 121. The isolate produce 1,4 cm of chitinolitic index after 96 hour incubation. Result of statistical test show both citin source type, pH of media production treatment and interaction were not significantly different (P˃0,05). KSR 121 isolate experience the highest growth of crab chitin treatment pH 8 (K2P3) with 6 hour incubation, whereas highest kinitase activity happen on crab chitin treatment pH 7 (K2P2) with 24 incubation, in amount of 0,125 (U/mL). Key words: N-acetil glucosamin, chtinase activity, chitinase, chitin, chitinolitic bacteria, isolation
Aktivitas Inhibitor α-Amilase Ekstrak Etanol Tanaman Brotowali (Tinospora crispa L.) Pujiyanto, Sri; Wijanarka, W; Raharjo, Budi; Anggraeni, Via
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.028 KB) | DOI: 10.14710/bioma.21.2.91-99

Abstract

Brotowali plant (Tinospora crispa L.) is a traditional Indonesian medicinal plant which has many benefits including for diabetes drugs. Diabetes mellitus (DM) is a metabolic abnormality caused by an increase in blood sugar levels. The purpose of this study was to determine the activity of α-amylase inhibitors of Brotowali (T. crispa L.) ethanol extract of plants. Extraction is done by maceration followed by evaporation. The extract obtained was tested for α-amylase inhibitor activity. The α-amylase inhibition test is based on the breakdown of starch substrates into maltose and glucose which is then determined by spectrophotometer after administration of DNS. Tests are carried out on controls and samples. As a substrate is 0.5% starch solution in 100 ml of sterile aquades. The reaction mixture was incubated 25 ° C for 10 minutes. The reaction is stopped by adding 2 ml of 3,5-dinitrosalicylic acid (DNS). All the mixed solutions are then heated to 100 ° C for 5 minutes and allowed to cool. The change in color of the solution is then measured for its absorbance at a wavelength of 540 nm. As a comparison used a control test that did not use extract samples. The results of α-amylase inhibitor activity test showed that ethanol extract with a concentration of 1000 μg / mL had the highest inhibitory activity value of 95.06% compared to extract concentration of 500 μg / mL, 250 μg / mL, 125 μg / mL and 62.5 μg / mL. The results of testing the effect of substrate concentration showed that 0.5% starch concentration had the highest inhibitory value of 9.52% compared to 2%, 1% and 0.25% concentrations
Uji Aktivitas Inhibisi α-Glukosidase Isolat Bakteri Endofit Tanaman Duwet (Syzygium cumini L. Skeels) Sebagai Sumber Alternatif Antidiabetes Fatin, Nuhaul; Pujiyanto, Sri; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.289 KB) | DOI: 10.14710/bioma.20.2.165-169

Abstract

Diabetes mellitus (DM) is a metabolic disease contributes to the health problem in Indonesia. The inhibition of α-glucosidase is one of the mechanisms of antidiabetic treatment. α-glucosidase inhibitor can be found in the duwet plants (Syzygium cumini L. Skeels)  that have been used traditionally in Indonesia as antidiabetic drug. However, due to the insufficient quantity and the long harvesting time, the forthcoming appliclation of duwet is consideraly not promising. The natural tendencies of endophytic microorganisms are identicl to the host plant. In this case the endophytic bacteria of the duwet plant is studied to determine its potential as an alternative to producing α-glucosidase inhibitors. A total of 14 isolates of endophytic duwet bacteria isolated tested the ability of α-glucosidase inhibitors using p-nitrophenyl-D-α-glucopyranoside. The result of  the absorbance based on breakdown of substrate that produce colored product and analyzed by spectrophotometric technique. All isolates had α-glucosidase inhibitor activity, the highest activity produced by isolates A21 and A22 with value of 69,18% and 69,22%. Keywords : inhibitor α-glucosidase, endophytic bacteria, Syzygium cumini
Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid) Hanafi, Ahmad; Purwantisari, Susiana; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (77.909 KB) | DOI: 10.14710/bioma.19.1.76-82

Abstract

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different. Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH
Identifikasi Jenis Pigmen Dan Uji Potensi Antioksidan Ekstrak Pigmen Bakteri Serratia marcescens Hasil Isolasi Dari Sedimen Sumber Air Panas Gedong Songo Naufal, Adhitya; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 2, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.736 KB) | DOI: 10.14710/bioma.19.2.95-103

Abstract

Pigments can be produced by plants, animals, and microbes, including the bacteria Serratia marcescens. Pigments are believed to be used as antibacterial, anticancer, antibiotic and antioxidant. The quality of the pigment is greatly determined by its type. The aim of this study was to identify the type of pigment produced by S. marcescens resulting from the sediment of Gedong Songo hot spring, and testing its antioxidant activity. S. marcescens was grown on Nutrient Broth (NB) medium for 96 hours at room temperature and sampled once every 6 hours for growth measurement and pigment measurement. Cell dry weight was used to measure the growth of S. marcescens, while the measurement of pigment production was done using spectrophotometer with 535 nm wavelength for red pigment and identification of pigment with Thin Layer Chromatography and UV-Vis and testing of antioxidant activity using carotene bleaching method. The results showed the measurement of pigment production has optimal point at 48 hours at 1,319 mg/L. Identification of pigment type S. marcescens using TLC obtained value of  0,8 with spectrophotometer wavelength at 536 nm. The value of antioxidant activity Pigment S. marcescens obtained at 13%. Keywords: Serratia marcescens, prodigiosin, KLT, antioxidant
Eksplorasi Jamur Alkalotoleran dari Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura, Jawa Timur Lusiana, Helga; Rukmi, MG Isworo; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3031.181 KB) | DOI: 10.14710/bioma.16.1.10-17

Abstract

Mold exploration from limestone hills West Sukolilo  village, District Labang, Bangkalan, Madura, East Java,  conducted to found the  alkalotolerant mold isolates and investigate their  amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method  on  PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of  four genus  i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test  showed Trichoderma longibrachiatum (IMD-26) have the  highest activity at pH 8, while  Fusarium sp 2 (IMD-24) showed the highest activity at pH 9. Aspergillus flavus (IMD-7) showed highest proteolytic activity at pH 8, while Hyphomycete (IMD-27) showed the highest activity at pH 9. The highest cellulolytic activity at pH 8 showed by Trichoderma harzianum (IMD-25) and pH 9 by Fusarium sporotrichoides (IMD-20). Keyword : alkalotoleran mold, amylolytic, proteolytic, cellulolytic.
Aktivitas Antibakteri Ekstrak Etanol dan Etil Asetat Batang Tanaman Brotowali (Tinospora crispa, L. Miers) terhadap Bakteri Escherichia coli Enteropatogenik (EPEC) Penyebab Penyakit Diare Fathmah, Ema Nuzula; Pujiyanto, Sri; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.044 KB) | DOI: 10.14710/bioma.21.1.1-8

Abstract

Diarrheal diseases is an endemic desease in Indonesia and potential desease of Kejadian Luar Biasa (KLB) which often leads to death. Enteropatogenic Escherichia coli (EPEC) is one of pathogenic bacteria which can cause diarrhea. Brotowali plant (Tinospora crispa, L. Miers) is one of traditional medicinal plants which is widely used to treat various diseases and and relatively have no side effects than chemical drugs. he purpose of this research is to know the antibacterial activity of Brotowali (Tinospora crispa, L. Miers) stems ethanol and ethyl acetate extract in inhibiting the growth of EPEC bacteria causing diarrheal disease. Brotowali extract was obtained by maceration method using ethanol and ethyl acetate solvent. The condensed extract obtained was dissolved in DMSO 100% until the concentration of the extract to 20%; 40% and 60%. Test of antibacterial activity used diffusion method using paperdisk. The ethanol and ethyl acetate extract of brotowali stems were each tested on EPEC bacteria. The results in this study are the inhibition zone on ethanol and ethyl acetate extract stem of brotowali plant to EPEC bacteria. The antibacterial activity of brotowali stem ethanol extract at 60% concentration showed the best effect compared to the concentration of 20% and 40%. The antibacterial activity of ethyl acetate extract of brotowali stem was as good at concentration of 20%; 40% and 60%. The antibacterial activity of brotowali stem ethyl acetate extract showed better results than brotowali stem ethanol extract
Co-Authors Achmadi Priyatmojo Ahmad Hanafi Aldila Riana Prabawati, Aldila Riana Andriana Sari Anggraeni, Via Cahya Buana Catur Arif, Catur Djoko Wahyono Djoni Haryadi Setiabudi Endang Kusdiyantini Fajar Agustini, Fajar Fathmah, Ema Nuzula Fatin, Nuhaul Febriani Tri Pamungkas Gregorius Satia Budhi Helga Lusiana, Helga Hera Widyastuti Hermin Pancasakti Kusumaningrum Herusugondo Herusugondo Herwenita, Herwenita Istiar Istiar, Istiar Kurniawati, Marina Lukman Hakim MG Isworo Rukmi Muhammad Amal Nurhakim, Muhammad Amal Muhammad Zainuri Nafisah, Hidayatun Naufal, Adhitya Sri Damranti Sri Pujiyanto Suprihadi Suprihadi Susiana Purwantisari Sutrisno, Sutrisno Ugung Dwi Ario Wibowo Ummatus Solikhah, Ummatus Wahju Herijanto Wahyu Utaminingrum Wijanarka, W