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PERSENTASE NIRA LONTAR (Borassus flabellifer L) DALAM PENGENCER TRIS - KUNING TELUR TERHADAP KUALITAS SEMEN CAIR KAMBING PERANAKAN ETAWAH YANG DISIMPAN PADA SUHU 3 - 5 °C Aleksander Kaka; W Marlene Nalley; Petrus Kune; Burhanuddin burhanuddin
JURNAL NUKLEUS PETERNAKAN Vol 1 No 1 (2014): Juni 2014
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v1i1.701

Tujuan dari penelitian ini adalah untuk mempelajari pengaruh suplementasi nira lontar dalamkuning telur Tris-(TEY) pengencer pada kualitas blasteran kambing peranakan etawah (ECB)semen disimpan pada 3-5oC. Semen dikumpulkan dari dua ECB jantan, menggunakan vaginabuatan dua kali seminggu. Semen dievaluasi makro dan mikroskopis. Sperma menunjukkan >70%sperma motil dibagi menjadi empat alikuot dan masing-masing diperpanjang dengan TEY (T0),90% TEY + 10% nira lontar (T1), 80% TEY + 20% nira lontar (T2) dan 70% TEY + 30% niralontar (T3) dan masing-masing disimpan di 3-50 oC. Hasil penelitian menunjukkan bahwa motildan sperma hidup pada T0 (50,83 ± 6.65; 63,04 ± 4,24) dan T1 (44,17 ± 9,17; 54,12 ± 7.85) dicapaipada hari ke-5 secara signifikan lebih tinggi (P <0,05) dibandingkan dengan T2 (44,17 ± 9.17 ;44,68 ± 5,61) dan T3 (50,83 ± 6.65; 37,46 ± 10,92). Disimpulkan bahwa T0 dan T1 adalahextender yang lebih baik untuk mempertahankan motilitas dan sperma hidup blasteran kambingperanakan etawah

TAMPILAN KINERJA REPRODUKSI PADA TERNAK BABI BETINA PERANAKAN LANDRACE DAN PERANAKAN DUROC Maria Hermigilda Ohin; Petrus Kune; Johny Nada Kihe
JURNAL NUKLEUS PETERNAKAN Vol 1 No 2 (2014): Desember 2014
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v1i2.756

This research was conducted to know the performance of female parent sow that being petted at the famer’s. This research use The Landrace’s the cross breed and Duroc’s cross breed available twenty females therefore overall 40 experimental units. This research also used one Duroc’s male pig as fresh sement resources for insemination. This observational data analysed by t -Test statistical analysis and the parameters were estrus duration (estrus period), Service per Conception, Conception Rate and Litter Size. The result of this research indicate that estrus duration of Landrace’s cross breed and Duroc’s are 3.25 days and 3.3 days, Service per Conception (S/C) 1.05 times and 1.15 times, Conception Rate 95% and 85% and Litter Size 10.00 tails and 8.88 tails. The results indicated that: there are no differences (P>0.05) on reproduction performance such as service per conception (S/C), conception rate and litter size. ABSTRAK Suatu penelitian telah dilakukan untuk mengetahui Kinerja dari babi - babi induk betina (Peranakan Landrace dan Peranakan Duroc) yang sedang dipelihara di tingkat peternakan rakyat. Penelitian ini menggunakan babi Peranakan Landrace dan Peranakan Duroc masing - masing sebanyak 20 ekor betina sehingga terdapat 40 satuan unit percobaan. Selain itu digunakan pula 1 ekor babi jantan Peranakan Duroc sebagai sumber semen segar untuk inseminasi buatan terhadap babi - babi dalam penelitian ini. Data hasil penelitian ini dianalis dengan analisis statistik Uji-T dan Parameter yang diamati yaitu: lama berahi, jumlah layanan per kebuntingan /Service per Conception (S/C), angka kebuntingan/ Conception Rate (CR) dan jumlah anak yang dihasilkan/Litter Size. Hasil penelitian ini menunjukan bahwa lama berahi babi Peranakan Landrace dan Peranakan Duroc yaitu 3.25 hari dan 3.3 hari, Service per Conception (S/C) 1.05 kali dan 1.15 kali, angka kebuntingan 95 % dan 85 %, serta Litter size 10.00 ekor dan 8.88 ekor. Hasil analisis statistik menunjukan bahwa lama berahi, Servie per Conception (S/C), angka kebuntingan dan litter size berbeda tidak nyata (P > 0.05).

HUBUNGAN SKOR KONDISI TUBUH DAN BERAT BADAN INDUK SAPI BALI DENGAN BERAT LAHIR DAN BERAT BADAN PEDET UMUR SATU BULAN Herpi A. Tainmeta; Petrus Kune; Winfrit A. Lay
JURNAL NUKLEUS PETERNAKAN Vol 3 No 1 (2016): Juni 2016
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v3i1.782

Twenty-four dams Bali cows (8-9 months of pregnancy) paired with each calf were used in this study., to investigate the correlation between body condition score and body weight of Bali cattle along with calf birth weight and calf body weight aged one month. Body condition score was determined by observation and palpation on the spinossus, and the scores were assigned based on the amount of fat reserves in the animals (1 = least fat/too thin; 2 = thin; 3 = medium; 4 = fat; 5 = most amount of fat). Cows samples were taken purposively with manual pregnancy test with palpation technic. Calves samples were taken accidentially ofther the cows giving birth. Data was analised by simple correlation of Karl’s Pearson.There was a negative correlation between body condition score of the dams and their calf birth weight aged one month (r = -0,085) observed in the present study. However, the dams with higher body weight had a positive correlation (r = 0,361) with calf birth weight after born as well as with calf birth weight aged one month (r = 0,320). The results highlight the important role that body condition score has no correlation with calf birth weight. ABSTRAK Tujuan penelitian ini yaitu untuk mengetahui ada tidaknya hubungan antara skor kondisi tubuh dan berat badan induk sapi Bali saat setelah beranak dengan berat lahir dan berat badan pedet umur satu bulan. Materi yang digunakan dalam penelitian ini adalah 24 ekor induk dengan kisaran umur kebuntingan 8-9 bulan dan 24 ekor anak sapi dari induk – induk tersebut. Skor kondisi tubuh (SKT) ditentukan dengan observasi dan palpasi pada spinossus, (SKT 1 = sangat kurus; 2 = kurus; 3 = sedang; 4 = gemuk; 5 = sangat gemuk). Sampel induk diambil secara purposif dengan cara melakukan pemeriksaan kebuntingan (PKB) dengan tehnik palpasi. Sampel pedet diambil secara Accidential sampling yang terlahir dari induk – induk sampel. Data yang diperoleh dalam penelitian ini dianalisis dengan menggunakan rumus korelasi sederhana dari Karl’s Pearson. Hasil penelitian menunjukkan bahwa : 1) antara skor kondisi tubuh induk dengan berat lahir pedet berkorelas inegatif dengan keeratan sangat lemah (r = -0,085); 2) antara skor kondisi tubuh induk dengan berat badan pedet umur satu bulan berkorelasi positif dengankeeratan lemah (r = 0,361); 3) antara berat badan induk dengan berat lahir pedet berkorelasi positif dengan keeratan sedang (r = 0,575);dan 4) antara berat badan induk dengan berat badan pedet umur satu bulan berkorelasi positif dengan keeratan lemah (r = 0,320).

PERFORMA REPRODUKSI SAAT LAHIR DAN SAPIH DARI INDUK KAWIN DENGAN PEJANTAN DUROC DAN LANDRACE (Reproduction performance at birth and weaning age of sows mating with duroc and landrace studs) Yohanes Djego; Petrus Kune; Johny Nada Kihe
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.2993

This research aim was to know reproduction performances at birth and weaning age of the sows which were mated with Duroc and Landrace studs. The research was carried out in two breeding farms namely the Instalation of Pig Breeding, the village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City. There were two breeds of studs namely breed of Landrace and Duroc which each stud was mated to 15 breeding pigs. The method used in this research was a survey and the samples were collected purposively. Data collection were obtained when the piglets were born and weaned. The variables were the litter size and body weight at birth age and the liter size and body weight at weaning age. The data obtained was analysed using the t test (t-test). The results showed that the characters of birth litersize,birth body weight, weaning litter size and weaning weight for Duroc stud were 10.60±2.41 head, 1.95±0.18 kg/head; 10.00±2.02 head and 5.98±0.65kg/head, respectively and those charackters for Landarace stud were 9.06±2.09head, 1.98 ±0.22 kg/head; 8.93± 2.23 head and 6.20±0.28 kg/head, respectively. Results of statistical analysed showed that Duroc and Landrace studs did not effect significantly (P>0.5) for those charackters. Reproduction performances of sows that were mated with Duroc and Landrace studs for characters of birth liter size, birth weight, weaning liter size and weaning weight were relatively similar.

Tampilan jumlah anak sekelahiran, bobot badan dan ukuran tubuh saat lahir pada anak babi induk peranakan landrace Yohanes Djego; Petrus Kune; Johny Nada Kihe
JURNAL NUKLEUS PETERNAKAN Vol 8 No 1 (2021): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i1.4226

This aim of the research was to know production performances at birth age of piglets of landrace sow crossbreds. The research was carried out in two breeding farms namely the Instalation of Pig Breeding, village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City. There were 15 heads of landrace crossbreed sows. The method used wasa survey and samples were collected purposively that there were all of the landrace sow crossbreds. The variables were litter size, body weights, girth size, and body length. Data were differentiated between males and females. The data obtained was analysed using the t test (t-test). The results showed that males had the average of litter size, body weight, girth size and body length were 5.03±1.81 head; 1.48±0.58 kg/head; 24.82±3.29 cm and 22.21±4.18 cm, respectively while females had the average of liter size, body weight, girth size and body length were 4.80±1.49 head; 1.45±0.49 kg/head; 24.51±3.17cm and 21.78±4.75 cm, respectively. Statistical analysis showed that there was no difference (P>0.05) between males and females from all observation variables. Performances of piglets from ;andrace sow crossbreds for male and female characters such as litter size, birth weight, girth size and body length were relatively similar.

KUALITAS SPERMATOZOA KAMBING BLIGON DALAM PENGENCER TRIS KUNING TELUR DENGAN PENAMBAHAN BERBAGAI LEVEL EKSTRAK KULIT BUAH NAGA Maria F. T. Leyn; Henderiana Belli; Wilmientje Marlene Nalley; Petrus Kune; Thomas Mata Hine
JURNAL NUKLEUS PETERNAKAN Vol 8 No 1 (2021): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i1.4230

The purpose of this study was to examine the effect of adding various concentrations of dragon fruit peel extract (DFPE) in Tris-egg yolk diluent (TEY) on the quality of bligon goat spermatozoa. Semen was collected twice a week using the artificial vaginal method from three year old bligon male goats with normal body conditions and reproductive organs. After the macroscopic and microscopic evaluation, good quality semen with a sperm motility of > 70%, a concentration of > 800 x 106 mL-1, and abnormality of < 15 %, was diluted with TEY + DFPE at a concentration of 0% (P0), 2% (P1), 4% (P2), 6% (P3), 8% (P4), dan 10% (P5). The semen which still had a motility ≥ 70% was then stored at a temperature of 3-5oC. Evaluation of the quality of spermatozoa was carried out every 24 hours until the motility decrease to 40%. The results showed that from day 1 to day 5 of preservation, spermatozoa preserved in TEY + 6% DFPE diluent (P3) had higher motility and viability (P<0,05) than other treatments. The abnormalities of spermatozoa until the 5 day of preservation were below 6%, but showed a significant difference (P<0,05) between treatments that received DFPE suplemention with control (P0). It was concluded that the addition of DFPE in TEY up to 6% could reduce the decrease in sperm quality of bligon goats during in vitro preservation.

EFEK KELAMIN TERHADAP PERFOMA LAHIR ANAK BABI INDUK PERANAKAN DUROC (Effect of sex on birth performace of piglets from duroc crossbred sows) Yohanes Djegho; Petrus Kune; Johny Nada Kihe
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.5389

This aim of the research was to know the influence of sex on production performances at birth age of piglets of duroc sow crossbreds. The research was carried out in two breeding farms namely the Instalation of Pig Breeding, village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City, East Nusa Tenggara (NTT). There were 20 heads of duroc crossbred sows. The method used a survey and samples were collected purposively. Samples were all pigles that born during research period from all of duroc sow crossbreds. The variables were litter size, body weight, girth size, and body length. Data was differentiated between males and females and analysed using the t test. The results showed that males had the average of litter size, body weight, girth size and body length of 4.94±1.21 head; 1.42±0.55kg; 23.94±2.80cm and 21.91±4.06cm, respectively; while females had the average of liter size, body weight, girth size and body length of 4.60±1.32 head; 1.39±0.38kg; 23.20±2.76cm and 20.93±4.20cm, respectively. Statistical analysis showed that there was no difference (P>0.05) between males and females from observation variables. Performances of piglets from duroc sow crossbreds for male and female characters such as litter size, birth weight, girth size and body length were relatively similar.

The Estimate of Repitability of Performances at Birth and Weaning Age of Duroc Crossbred Sows Yohanes Djego; Johny Nada Kihe; Petrus Kune
Journal of Tropical Animal Science and Technology Vol 4 No 1 (2022): Journal of Tropical Animal Science and Technology
Publisher : Program Studi Peternakan, Fakultas Pertanian Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v4i1.2185

The aims of this study were to determine the average and standard deviation and to estimate repitability coefficients of production at birth and weaning characters of duroc crossbred sows. The study was carried out in two breeding farms namely the Instalation of Pig Breeding, village of Tarus, Kupang Regency and the Manise Pig Farm, Village of Oetete, Kupang City, East Nusa Tenggara (NTT). Materials used duroc crossbred sows ( 15 heads ) which born and weaned piglets during the study periode. All sows was mated artivicialy with boars of duroc crossbred. Variables were liter size, weaning rate, birth weight and weaning weight. Analysis of statistical description was used to determine the average and deviation standard of liter size, weaning rate, birth and weaning weights. Effects between paritas was analysed using t-test and the interclass correlation was used to calculate the coefficient of repitability. Results showed that duroc crossbred sows had the average and deviation standard for the first and second liter size 9.87±1.80 head and 10.67±2.58 head; for the first and second birth weight 1.30±0.26 kg and 1.47±0.47 kg, for the first and second weaning weight 6.01±0.49 kg and 6.47±0.61 kg .and for the first and second weaning rate 8.20±1.78 head and 8.53±2.23 head; respectively. The average of litter size, birth weight, weaning weight and weaning rate between the first and second parity was no difference (P > 0,05). The coefficients of repeatabitily for characters of liter size, birth weight, weaning weight and weaning rate were 0.48; 0.30; 0.46 and 0,52; respectively. In conclution, the coefficients of repeatabily of litter size, birth weight and weaning weight from duroc crossbred sows were medium and that for weaning rate was high value and the performance of characters between the first and second parity were relatively same.

EFFECT OF PROGESTERONE AND HCG AFTER ARTIFICIAL INSEMINATION ON PREGNANCY RATES AND PROGESTERONE LEVELS IN BALI TIMOR COWS Henderiana L.L. Belli; Filemon Y. Lanata; Petrus Kune; Imanuel Benu
JURNAL NUKLEUS PETERNAKAN Vol 9 No 1 (2022): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v9i1.7282

This study determined whether the administration of progesterone and hCG after insemination increased pregnancy rate in Bali Timor cows. A total of 30 Bali Timor cows (BW ± 200-225 kg; aged 3-7 years old) with different parity were used in this study. Cows were inseminated with progesterone and hCG. Cows were grouped based on body weight and number of parities following a Complete Randomized Design with 3 treatments and 10 replications. The treatments were: P1 = did not receive any treatment (control); P2 = estrus induced by PGF2α 125 mg on day 5, 7 and 9 (n = 10); and P3 = estrus induced by hCG 1000 IU on day 5 after artificial insemination (AI). Blood samples were collected from the jugular vein of the animals on day 0 and continued for a period of 33 days after AI using 5 mL heparinised pre-set syringes Blood samples were then centrifuged at 1500 rpm for 10 minutes. Serum was harvested and stored at -20oC until serum concentrations of progesterone and hCG were determined using ELISA procedures. Pregnancy was determined by rectal palpation. The results showed that the AI pregnancy rates was greater in group treated with hCG (70%) than cows induced with progesterone (50%) or the control group. The concentrations of both progesterone and hCG were increased simultaneously on day 5 to 11 post AI. In conclusion, progesterone and hCG application in Bali Timor cows after artificial insemination could increase pregnancy rates.

KUALITAS SPERMA SAPI ANGUS DALAM PENGENCER CITRATE-KUNING TELUR YANG DITAMBAHKAN SARI BUAH TOMAT (Sperm quality of angus bulls in citrate - egg yolk extender added tomato juice) Valerianus L. Janggur; Thomas Mata Hine; Petrus Kune
JURNAL NUKLEUS PETERNAKAN Vol 10 No 1 (2023): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v10i1.8347

Preservation is a technology created to keep sperm quality over a specific time period, which is subsequently used for artificial insemination. Attempts have been made to reduce the loss of sperm quality during storage by diluting the sperm with materials containing various nutritional components required by sperm to preserve motility and vitality during storage. This study aimed to analyze the impact of adding tomato juice (TJ) to citrate–egg yolk (CEG) diluent on the sperm quality of Angus bulls. One three-year-old angus bull was collected using an artificial vagina, and good quality sperm was preserved in CEG diluent with various levels of TJ added: 0, 1, 2, and 3% for treatments P0, P1, P2, and P3 sequentially. After dilution, the sperm were stored in the refrigerator with a temperature range of 3-5oC. Sperm quality was evaluated every 24 hours until motility reaches 40%. The results of the study up to the fifth day of storage showed significant differences (P<0.05) in the variables of sperm motility, viability, and survival, but there were no significant differences in the variable of sperm abnormalities (P>0.05) between the P2 and P3 with control. The following sperm quality comparisons were made in the P3 vs. control treatment: motility (50.00 vs. 36.50%), viability (54.84 vs. 47.35%), abnormality (7.35 vs. 7.07%), and survival (5.83 vs. 4.50 days). The study concluded that adding 2-3% TJ in the CEG diluent could sustain the sperm quality of Angus bulls.

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