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PENGARUH PENAMBAHAN SARI WORTEL DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING BLIGON (The effect of carrot juice supplementation in citrate - egg yolk extender on spermatozoa quality of bligon goat) Maria Epifania Barek; kirenius uly; Thomas Mata Hine; Wilmientje Marlene Nalley; Henderiana L.L. Belli
JURNAL NUKLEUS PETERNAKAN Vol 7 No 2 (2020): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v7i2.3152

Abstract

This study aims to determine the effect of adding various doses of carrot juice into citrate-egg yolk diluents (C-EY) on bligon buck semen quality. Semen was collected twice a week using an artificial vaginal method of three buck with normal body condition and reproductive organs. Semen was 70% of motility, konsentration ≥1000x106mL and abnormality ≤ 15 diluted with C-EY added with carrot juice at a concentrations: 0% (CJ-0), 10% (CJ-10), 12.5% (CJ-12,5), 15% (CJ-15), 17.5% (CJ-17,5), and 20% (CJ-20), and stored in a refrigerator at 3-5o C. The quality of spermatozoa was evaluated every 24 hours. The Variables measured were spermatozoa motility, viability, and abnormalities. The results showed that spermatozoa preserved by C-EY supplemented by 17.5% carrot juice (CJ-17.5) had a higher quality (P<0.05) compared to the other four treatments, with motility (49.04 ± 1.0%), viability (55.70 ± 1.22%), dan abnormalities (4.57 ± 0.88 %) on the 4th day of storage. This study concludes that 17.5% carrot juice into citrate eggyolk extender improves the bligon buck semen
PENGARUH PENAMBAHAN FILTRAT ROSELLA (Hibiscus sabdariffa Linn) KE DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING KACANG (The effect of rosella filtrate suplementation in tris-egg yolk extender on sperm..... Reynold Laos; Aloysius Marawali; Petrus Kune; Henderiana L. L. Belli; Kirenius Uly
JURNAL NUKLEUS PETERNAKAN Vol 8 No 2 (2021): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v8i2.4872

Abstract

The purpose of this study was to determinate the effect of adding various levels of roselle filtrate (RF) into tris-egg yolk diluents (T-EY) on the quality of kacang goat spermatozoa. Semen was collected twice a week using an artificial vagina method of three male goats. Fresh semen obtained was evaluated macroscopically and microscopically with a motility value of 80%, viability 86.58%, concentration of 2,099.40 milionspermatozoa cells/ml, and an abnormality of 2.83%. Then the fresh semen was diluted according to the treatment, namely T0 (T-EY 100%), T1 (T-EY 100% + RF 1%), T2 (T-EY 100% + RF 2%), T3 (T-EY 100% + RF 3%), T4 (T-EY 100% + RF 4%), T5 (T-EY 100% + RF 5%). The diluted semen was stored at 3-5C, and evaluation was carried out every 24 hours on the quality of the spermatozoa. The results showed that spermatozoa in T-EY diluent with the addition of 3% RF (T3) had a higher quality (T<0.05) compered to the other five treatments, namely the motility reached (42,99±2,19%), viability (49,30±2,24%), and abnormalities (4,012±0,49%) during 5 days of storage. The conclusion of this study is the addition of 3% roselle filtrate in tris-egg yolk diluent is the best level and is more effective in maintaining the quality of spermatozoa kacang goat.