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All Journal JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI
Rita Dwi Rahayu
Puslitbang Biologi LIPI, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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PERUBAHAN KADAR KOLESTEROL SERUM PADA TIKUS SETELAH MENGKONSUMSI MALTOOLIGOSAKARIDA YANG DISINTESIS SECARA ENZIMATIK MENGGUNAKAN AMILASE Bacillus licheniformis BLl [The Change of Serum Cholesterol Level in Rats after Consuming Maltooligosaccharide Synthesized by Enzimatic Reaction of Bacillus licheniformis BLl Amylase] Dinoto, Achmad; Rahayu, Rita Dwi; Satyaningtijas, Aryani S
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1007.167 KB) | DOI: 10.14203/beritabiologi.v10i1.2046

Abstract

To investigate the impact of consuming enzymatically synthesized maltooligosaccharide using Bacillus licheniformis BLl amylase on reduction of serum cholesterol level, in preclinical study groups of Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (BD), high serum cholesterol-stimulating basal diet (CD), high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) commercial maltooligosaccharide (CI), and high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) maltooligosaccharide synthesized by enzimatic reaction of B. licheniformis BLl amylase (CB).Our result shows a reduction of serum cholesterol level up to 39.1% in rats CB after three weeks consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase. In addition, significantly increased blood glucose level was not found in this study as an impact of consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase.
KOMUNITAS MIKROBA PENGAKUMULASI GLIKOGEN Supriyati, Dyah; Rahayu, Rita Dwi; Imamuddin, Hartati
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i5.1896

Abstract

Activated sludge originated from anaerobic-aerobic process waste water treatment plan was acclimated with glucose and acetate.The experiment was conducted in 1 L working volume of sequential batch reactor (SBR) that was adjusted to 0, 25 kg m day.Glucose was effectively utilized by microbial community in anaerobic condition, and glycogen synthesis was occurred in aerobic condition.Suppression of polyphosphate accumulating organism was caused by the domination of glycogen accumulating organism and the high of nitrate production. In order to accelerate the community polyphosphate accumulating organisms then to the competitor of these communities must be pressured.
EFEKTIVITAS ALKOHOL DAN CAMPURANNYA DENGAN FORMALIN SEBAGAI BAHAN PENGAWET SPESIMEN BINATANG UNTUK UDANG Suharna, Nandang; Rahayu, Rita Dwi
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.382 KB) | DOI: 10.14203/beritabiologi.v5i1.1099

Abstract

This study was aimed at studying the effectiveness of alcohol, formaldehyde and their mixture in inhibiting fungal growth.This study contained two step. The first step was fungal isolation from specimen.One isolate was then selected to be inoculated into alcoholat various concentration (15%, 30%, 50% and 70%) with or without formaldehyde mixture.The second was fungal inoculation intoalcohol at various concentration (15%, 30%, 50%, dan 70%) with or without formaldehyde.The effectiveness of these subtances as specimen preservatives for shrimp were studied after six months incubation time.From the fisrt step, only genus group Monascus was isolated from seven specimens(Mabuia multifasciata, Myotis muricola,Chironax melanocephalus,Naya naya sputratrix, Miniopterus schreibersi blepotis,unidentified shrimp, unidentified Coelenterata).From the second step, the best growth of isolate Monascus sp.MM which isolated from the fisrt step was achieved in 70% alcohol.This fungus was not able to grow in both formaldehyde and its mixture with alcohol (1:1, 1:2, 1:3 and 1:4). The results also showed that Monascus growth was observed in alcohol with not sterilized shrimps without inoculation with Monascus sp. MM. It is recommended to apply mixture alcohol 70% with formaldehyde 1% at ratio 4:1 as animal specimen preservative to inhibit fungal growth.
PURIFIKASI SENYAWA POLIFENOL GLUKOSIDA HASIL REAKSI TRANSGLIKOSILASI ENZIMATIK DARI BIAKAN DAN UJI AKTIVITASNYA SEBAGAI ANTIMIKROBA Handayani, Rini; Rahayu, Rita Dwi; Sulistyo, Joko
Jurnal Teknologi Lingkungan Vol. 13 No. 2 (2012)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jtl.v13i2.1420

Abstract

Polifenol-glukosida disintesis menggunakan CGTase yang berasal dari Nocardia sp. Sintesis polifenol-glukosida dilakukan dengan menggunakan resorsinol sebagai akseptor dan tepung sagu sebagai donor. Penelitian ini bertujuan untuk mensintesis senyawa polifenol-glukosida secara enzimatik menggunakan CGTase dari biakan Nocardia sp,menguji aktivitasnya sebagai senyawa antimikroba dan untuk mengetahui pengaruh senyawa polifenol-glukosida terhadap kerusakan morfologi sel dari biakan Bacillus subtilis. Polifenol–glukosida hasil reaksi transfer dimurnikan menggunakan kolom kromatografi yang berisi matriks oktadesil silica dan menggunakan eluen asam formatdalam methanol (40-90%). Produk yang sudah terpisah ditunjukkan sebagai noda tunggal pada plat kromatografi lapis tipis dengan nilai Rf mendekati nilai Rf standar arbutin. Nilai Rf dari produk transfer tersebut adalah sebesar 0,84 dan nilai Rf standar arbutin adalah sebesar 0.85. Polifenol-glukosida hasil sintesis menunjukkan aktivitasantimikroba terhadap biakan Bacillus subtilis dan Escherichia coli. Kata kunci : polifenol-glukosida, CGT-ase, Nocardia sp., Bacillus subtilis dan Escherichia coli AbstractPolyphenol-glucoside was synthesized by using CGTase derived from Nocardia sp. Synthesis polyphenol-glucoside of was done by using resorcinol as the acceptor and starch sago as the donor. This study aims to synthesized polyphenol-glucoside enzymatically using CGTase derived from Nocardia sp and to assay it’s activity as antimicrobial compound and to determine effect of polyphenol-glucoside on morphological damaging of Bacillus subtilis cells. The synthesized polyphenols-glucoside by transfer reaction was purified through column chromatography containing octadecyl silica matrix that was eluted with formic acid in methanol (40-90%). The separated product was demonstrated by single spot appearance on thin-layer chromatography plate with Rf value that was closed to standard of arbutin Rf. The Rf value of this transfer product was 0.84 while Rf value of arbutin as authentic standard was 0.85. The synthesized polyphenol-glucosie exhibited antimicrobial activity against Bacillus  subtilis and Escherichia coli. Key words : Polyphenol-glucoside, CGT-ase, Nocardia sp., Bacillus subtilis and  Escherichia coli.
Co-Authors A.S. Satyaningtijas Achmad Dinoto Hartati Imamuddin Joko Sulistyo Nandang Suharna, Nandang Rini Handayani Supriyati, Dyah