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All Journal Biopropal Industri BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Teknologi Indonesia Makara Journal of Science
NANIK RAHMANI
Research Center for Biotechnology,Indonesia Institute of Sciences, Jl Raya Bogor KM.46 Cibinong, Bogor, 16911, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology

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Journal : BERITA%20BIOLOGI

 1 
PURIFIKASI DAN KARAKTERISASI ENZIM PEKTINASE DARI ASPERGILLUS USTUS BL5 Yopi, Yopi; Rahmani, Nanik; Andriani, Ade; Dewi, Fitria; Meryandini, Anja
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.646

Abstract

Pectinase is an enzyme that could hydrolyze pectin into galacturonic acid. Natural pectinase was produced by microbes such as bacteria, yeast, fungi and Actinomycetes. Application of pectinase in industry were mainly in juice industry, textile, pulp, tea, cocoa and coffee fermentation. In this research, we conducted purification and characterization of pectinase produced by Aspergillus ustus BL5 in submerged fermentation using commercial pectin. The result showed that the optimum of pectinase production was reached at 120 hours fermentation process with specific activity 0.59 U/mg. The crude extract of pectinase was then concentrated using PEG 6000 and purified by Sephadex G-75 gel filtration chromatography. There were 2 fractions contained pectinase which the activity was 4.15 U/mg (pectinase A) and 3.3 U/mg (pectinase B), respectively. Compare to crude extract, the yield product of pectinase A and B increased 6.94 and 5.53 times, respectively. The purified pectinase A have optimum temperature at 50 oC and optimun pH at 5.
Co-Authors - Yopi -, Yopi - ADE ANDRIANI Ahmad Thontowi Anggraini, Yufi Sara Anja Meryandini Ashadi Sasongko Dewi, Fitria Endang Saepudin, Endang Nuryati Nuryati Palupi, Nurheni Sri PUSPITA LISDIYANTI Rahmasari, Dianti Rahmasari, Dianti Rohmattusolihat Rohmattusolihat, Rohmattusolihat Ruth Melliawati Sari, Yana Nurita Sari, Yana Nurita Sri Hartati Sri Pujiyanto Wijanarka Wijanarka YOPI YOPI Yopi,