Sri Mumpuni Sosiawati
Department of Veterinary Parasitology - Faculty of Veterinary Medicine, Universitas Airlangga

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The Anthelmintic Activity of Ethanol Extract of Basil Leaves (Ocimum americanum Linn.) Againt Haemonchus contortus Death in Vitro Rari Noviana; Chairul Anwar Nidom; Agus Sunarso; Setiawan Koesdarto; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.906 KB) | DOI: 10.20473/jops.v1i2.16289

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui daya anthelmintik dari ekstrak etanol daun kemangi (Ocimum americanum Linn.) terhadap mortalitas cacing Haemonchus contortus secara in vitro. Sediaan ekstrak dibuat dengan konsentrasi (berat/volume) 2,5%, 5%, 7,5%, dan 10%. Untuk kontrol positif digunakan PBS dan DMSO sedangkan untuk kontrol negatif digunakan Albendazole 10 mg/ml. Sebanyak 10 ekor cacing Haemonchus contortus betina direndam dengan berbagai perlakuan dalam cawan petri. Masing-masing perlakuan diulang sebanyak 4 kali. Pengamatan dilakukan setiap 1 jam sekali selama 6 jam untuk melihat apakah cacing masih hidup atau sudah mati. Hasil dari penelitian ini menunjukkan bahwa ekstrak daun kemangi memiliki efek anthelmintik terhadap cacing Haemonchus contortus secara in vitro. Makin tinggi konsentrasi ekstrak, maka daya anthelmintiknya juga semakin tinggi. Pada ekstrak konsentrasi 10% memiliki kemampuan anthelmintik yang hampir sama dengan Albendazole.
Anthelmintic Potential Extract Mango Gadung Seed (Mangifera indica L.) Mecistocirrus digitatus in vitro Ria Nikmatul Jannah; Sri Mumpuni Sosiawati; Sri Chusniati; Kusnoto Kusnoto; Rahmi Sugihartuti; Setiawati Sigit
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.229 KB) | DOI: 10.20473/jops.v1i2.16290

Abstract

This research aim to attest the potential anthelmintic extract  mango seed gadung  (Mangifera indica L.) against worms Mecistosirrus digitatus in vitro, the study also aims to determine the effect of immersion time, variasis concentration and the relationship between time and treatment. this study used 210 M. digitatus worm extracted regardless of gender worms. treatment that is given was  the concentration  extract  mango seed gadung  was 5%, 7.07%, 10%, 14.14%, 20%, negative control using NaCl physiological  and comparison using levamisole Hidrokloride 0.0025 mL. Observations death worm Mecistocirrus digitatus and observations were made at the 6th hour, 12th hour, 18th hour and 24th hour. The results showed that variations in concentration, soaking time and the relationship between soaking time with the treatment affect mortality Mecistocirrus digitatus worms. Test results of the analysis statitistik using factorial Anova and Duncan's Multiple Range Test showed a significant difference (p<0.01) between the time factor, variations in concentration and relations between time and treatment.
The Effect of Calcium Hydroxideon Mortality of Ascaridia galli In Vitro Inggarsetya Syah Audini; Setiawan Koesdarto; Ira Sari Yudaniayanti; Setiawati Sigit; Sri Mumpuni Sosiawati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.427 KB) | DOI: 10.20473/jops.v2i1.16377

Abstract

Ascaridiasis can be happened in either broiler or hens that can caused decrease of meat and egg productions. Ascaridiasis is a parasite that is most often found in poultry and cause high economic losses. This research was conducted to determine the anthelmintic effect of calcium hydroxide [Ca(OH)₂] on mortality of Ascaridia galli in vitro. In this research used Ascaridia galli with length 7-11 cm without differentiating their sex. The concentrations of calcium hydroxide [Ca(OH)₂] for immersion the Ascaridia galli were 0,01%, 0,02%, 0,025% and 0,03% as treatment group, respectively. The negative control used aquadest. Observation death A.galli worm and analysis of the data at the 2 hour, 4 hour, 6 hour, 8 hour, 10 hour and 12 hour. The result showed that the increase of concentration increased mortality of Ascaridia galli. Percentage of death worm A.galli at the 12th hour on concentration ,01%, 0,02%, 0,025% and 0,03% was 20%, 55%, 75% and 100%. ANOVA test showed significant difference between control and treatment group (p<0.05). Duncan multiple range test was seen the best treatment was the biggest mortality in concentration of 0,03%.
Prevalence of Nematodiasis in Omasum and Abomasum of Goats Slaughtered at Pegirian Surabaya Slaughter House Through Gatrointestinal Surgery Method Dewi Mariyam; Sri Mumpuni Sosiawati; Kuncoro Puguh Santoso; Setiawan Koesdarto; Sarmanu Sarmanu; Ira Sari Yudaniayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.67 KB) | DOI: 10.20473/jops.v2i1.16384

Abstract

The purpose of this research is to determine the kind of nematodes and to determine the prevalence result. Thirtysix samples of omasum and abomasum were collected from RPH Pegirian from September-November 2017 then using gastrointestinal surgery to get fresh adult worm, afterwards using Semichen-Acetic Carmine stainning method to makes the object easy to microscopic identify. The result showed that species was found Haemonchus contortus, as an identify result show that adult worm has a character on a anterior part called cervical papilae, female  worm has a barber’s pole on body also posterior part has a vulva flap, posterior part of male worm has a bursa copulatrix, spicula, gubernaculum and dorsal rays looks like Y shape. Seventeen positive samples and the prevalence rate was 47,2%, 273 adult worms and female worms 215 (78,75%) and the male worms 58 (21,25%). Chi-Square statistical test (P <0.05) showed that 13 positive samples of kambing kacang (65%) were higher infected Haemonchosis infection than 4 potitive samples of PE (25%).
Identification of Gastrointestinal Worms Egg on Spotted Deer (Axis axis) and Bawean Deer (Axis kuhlii) at Wonorejo Bibit Park and Surabaya Flora Park Achmad Hasan Sahani; Sri Mulyati; Tri Bhawono Dadi; Sri Mumpuni Sosiawati; Kusnoto Kusnoto; Ratna Damayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.98 KB) | DOI: 10.20473/jops.v2i2.16403

Abstract

The purpose of this study was to identify the type of gastrointestinal worms egg of Spotted Deer (Axis axis) and Bawean Deer (Axis kuhlii) at Wonorejo Bibit Park and Surabaya Floral Park. The research was conducted in October-December 2017 used total 50 specimen of sample faeces, 40 sample faeces from Surabaya Flora Park and 10 sample faeces from Wonorejo Bibit Park, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Airlangga University. The results showed that the degree of helmint gastrointestinal in Bawean Deer and Spoted Deer at Wonorejo Bibit Park showed 0% while at the Surabaya Flora Park is 15%. The type of worm found were Trematoda class it was Fasciola sp. The resuls of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal helminth infection (p>0.05) .