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Rahmansyah Rahmansyah
Tadulako University
Computer Science & IT

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KETAHANAN BUAH BEBERAPA KLON KAKAO (THEOBROMA CACAO L.) TERHADAP INFEKSI PENYAKIT BUSUK BUAH BERDASARKAN UJI DETACHED POD Aisyah, Nurul; Rahmansyah, Rahmansyah; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 3, No 2 (2014): Volume 3 Number 2 (August 2014)
Publisher : Univ. Tadulako

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Abstract

One target on Cacao breeding program is to develop superior clone which resistance against major pest and diseases. In present time, Pod Rot disease is one of serious problem on the Cacao plantation system, including over the area of Central Sulawesi. The aim of this study was to identifiy the resistancy on five different clones of cacao (ICCRI 1, ICCRI 3, ICCRI 4, Local Sidondo and Green Local Sausu) from local farming in Central Sulawesi against Phytophthora pot rot infection based on detached pod assay. Sample were collected from the farming area of Sigi Biromaru, Palolo, and Sausu, over the harvesting period of February - May 2014. Mature and healthy pods were detached from the plant, then artificially inoculated by using Phytophthora-infected pericarp. Pod rot symptom and spreading area of fungi  were observed dialy, then total area of  infection on day-6 was used as disease rating scale. The outcome data showed that all pod of the clones were classified as a highly susceptible clones against pod rot disease. However, pod resistance level of each clones were statisfically different. Green Local Sausu and Local Sidondo were more resistant than other clones.  This result suggesting that Local clones have been developing resistancy mechanism against Pod Rot disease.
VARIASI GENETIK KLON KAKAO (THEOBROMA CACAO L.) DI DESA SAUSU PEORE KAB. PARIGI MOUTONG Rahmansyah, Rahmansyah; Muslimin, Muslimin; Mutmainah, Mutmainah; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 3, No 3 (2014): Volume 3 Number 3 (December 2014)
Publisher : Univ. Tadulako

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Abstract

Informasi tentang keragaman tanaman kakao sangat diperlukan untuk mendukung program pemuliaan dan pengembangan tanaman dalam meningkatkan produktivitas biji kakao. Untuk itu penting dilakukan identifikasi keragaman varietas pada berbagai daerah sentra produksi biji kakao. Penelitian ini bertujuan untuk mengetahui variasi genetik kakao klon 45, M 05, 06, Lokal Hijau Sausu dan Lokal Merah Sausu di desa Sausu Peore, Kab. Parigi Moutong. Identifikasi variasi genetik didasarkan pada teknik RAPD dengan menggunakan 4 macam primer yaitu TCL05, TCH05, AS9870, dan TCM20. Identifikasi juga dilakukan pada morfologi buah. Buah kakao diambil pada musim berbuah bulan Februari-Mei 2014. Hasil penelitian menunjukan bahwa kelima klon memiliki variasi genetik. Total pita DNA yang teramplifikasi sebanyak 17 pita. Tingkat polimorfisme sebesar 70,59% (12 pita polimorpic). Variasi genetik ini juga didukung oleh perbedaan morfologi buah pada masing-masing klon.
Co-Authors Ali Djamhuri I Nengah Suwastika Muslimin Muslimin Nurul Aisyah