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All Journal Ovozoa: Journal of Animal Reproduction

wurlina wurlina

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Author-ID : 3572250

Agriculture, Biological Sciences & Forestry Veterinary

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PENAMBAHAN L – ARGININ DALAM PENGENCER TRIS KUNING TELUR SETELAH EKUILIBRASI 2 JAM TERHADAP MOTILITAS DAN MEMBRAN PLASMA UTUH SPERMATOZOA DOMBA SAPUDI Hastini Suryaningsih; Emy Koestanty Sabdoningrum; Suherni Susilowati; Trilas Sardjito; wurlina wurlina; Tita Damayanti
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

This research was aimed to determine the addition of L-arginine on motility and sperm plasma membrane of Sapudi sheep after two hour equilibration time in egg yolk tris diluent. This research used fresh samples of sheep Sapudi semen collected by artificial vagina, then were devided into 4 treatments and 6 replications. Analysis of the data using Analysis of Variance (ANOVA) then proceed to the Duncan Test to determine significant differences between treatments. The first treatment P0 was no L – Arginine added as control. P1 was treated with 0,004 M L – Arginine. P2 was treated with 0,005 M L – Arginine, P3 was treated with 0,006 M L – Arginine. Result of two hour equilibration time for the sperm motility showed: (P0) 46,67a 5,16, (P1) 49,16ab 5,84, (P2) 55,00bc 7,74, (P3) 58,33c 6,83. Result of two hour equilibration time for the sperm plasm membrane were: (P0) 39,33a 4,92, (P1) 43,33ab 4,22, (P2) 46,50 bc 3,44, (P3) 50,83 c 3,48. The addition of L-arginine with a concentration as much as 0.006 M, shows the highest result in sperm motility and sperm plasm membrane intact of Sapudi semen.

PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP FOLIKULOGENESIS MENCIT (Mus musculus) Abdullah Abdullah; Tjuk Imam Restiadi; Nunuk Dyah Retno Lastuti; Tita Damayanti; wurlina wurlina; Erma Safitri
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbreed mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subject of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatment were K0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are number of primary, secondary, tertiary and de Graff follicles. During the treatment the estrus cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affect (p<0,05) on increasing of the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicle significantly (p<0,05).

UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN Silvia Rani Andriyanti; Mas’ud Hariadi; Roesno Darsono; Pudji Srianto; wurlina wurlina; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a 73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.

PENGARUH JARAK DAN LAMA WAKTU PROSES PENGHITUNGAN STRAW SEBELUM DISTRIBUSI TERHADAP KUALITAS SEMEN BEKU SAPI SIMENTAL POST THAWING Siti Nur Fatimah; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Aims of this research was to determine the effect of distance and the duration of straw count above liquid nitrogen to the Quality of Frozen Simental Cattle. This study used a sample derived from simmental cattle from BBIB singosari as much as 48 straw divided into 8 treatments. P1(0,5cm 15 sec), P2(0,5cm 30 sec), P3( 0,5cm 45 sec), P4(0,5cm 60 sec), P5(1,5cm 15 sec), P6(1,5cm 30 sec), P7(1,5cm 45 sec), P8(1,5cm 60 sec) and repeated as much as 6 times then observed for motility and viability of post thawing spermatozoa. The data obtained were analyzed using SPSS 16.0. The data obtained were analyzed using SPSS 16.0. The results showed that the duration of straw count and the position of straw from liquid nitrogen not significant (P>0,05) on post thawing motility. The position of straw from liquid nitrogen not signifant (P>0,05) on post thawing viability. The conclusion of this research showed that 0,5 cm 15 sec give the higher post thawing motility.

EFISIENSI REPRODUKSI SAPI PERANAKAN LIMOUSIN AKSEPTOR INSEMINASI BUATAN DI KECAMATAN TIKUNG, KABUPATEN LAMONGAN TAHUN 2016 Vina Ari Prastyorini; Koesnoto Supranianondo; Erma Safitri; Tjuk Imam Restiadi; wurlina wurlina; Tatik Hernawati
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was known the difference of reproductive performans Limousine Cross Breed that haved artificial insemination from diffrence straw from BBIB Singosari and BBIB Lembang covering Conception Rate, Service per Conception, and Calving Rate in period 2016th. This study uses survey research which data from primary and secondary. Primary data were obtained from interview with Limousine Cross Breed breeders and secondary data were obtained from artificial insemination officer’s record. The observed variables are Conception rate (CR), Service per conception (S/C) and Calving rate (CvR). Data were analyzed descriptive that average and Chi Square.Result of research showed no significant difference (p>0.05) on CR, S/C and CvR between Limousin cross breed cows that haved artificial insemination from BBIB Singosari and BIB Lembang.

PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii Paraswita Eindah Fitri; Wurlina Wurlina; Sri Chusniati; Lucia Tri Suwanti; Mufasirin Mufasirin; Hani Plumeriastuti
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.

POTENSI EKSTRAK BUAH PARE (Momordica charantia L.) TERHADAP JUMLAH SEL LEYDIG DAN HORMON TESTOSTERON MENCIT (Mus musculus) JANTAN Faradillah Hapsari Dwi Putri; Wurlina Wurlina; Benjamin Christoffel Tehupuring
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The active compound in bitter melon, i.e., flavonoid, is allegedly inhibiting the formation of testoterone by Leydig cell. The aim of the research was investigating the Leydig cell and testoterone level due to the addition of bitter melon extract (Momordica charantia L.). This research was conducted on December 2016 at the Animal Testing Lab of Faculty of Veterinary Medicine Universitas Airlangga, while the production of histopathology preparations was at the pathology laboratory of Universitas Airlangga Surabaya, and the execution of testosterone level test was at Balai Besar Laboratorium Kesehatan Surabaya. The utilized research design was Completely Randomized Design, exploiting 20 male house mice which are divided into four experimental groups of 5 male house mice each. Each group had given the treatments of bitter melon extract of 23mg/kgBB, 35mg/kgBB, 45,5mg/kgBB, while the control group had given the drug solvent of CMC Na 1%, which is conducted by utilizing feeding needles. In the 41st day, the male house mice were being euthanized by cervical dislocation. The Leydig cells could be observed by utilizing 400x microscope zoom, while the testosterone was examined utilizing Enzym Linken Immunosorbent Assay (ELISA). The research result demonstrated that the bitter melon extract (Momordica charantia L.) addition could decrease the Leydig cells and the testosterone level.

HUBUNGAN ANTARA UMUR DENGAN CALVING INTERVAL, DAYS OPEN, DAN SERVICE PER CONSEPTION SAPIFRIESIAN HOLSTEIN (FH) Hanifah Muslimah Ananda; Wurlina Wurlina; Nove Hidajati; Mas’ud Hariadi; Abdul Samik; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research was to know the relationship between age with calving inteval (CI), days open (DO), and service per conception (S/C) in Friesian Holstein dairy cattle (FH). The research was started on Desember 2017 to January 2018 in PT. Greenfields Indonesia partnerships, KecamatanWagir Kabupaten Malang. The materials of the search used were 100 heads of dairy cattle which had at least two times of parturition. The method used in this research was a survey and data collection. The data were obtained from the records of reproduction. Data analysis was multiple linear regression using SPSS software 21.0 version. The results showed that the values of CI (434,9±58,9 days, 449,4±66,2 days, and 431,8±59,2 days), DO (218,9±58,7 days, 218,9±58,7 days, dan 217,6±54,1 days), dan S/C (3,2±1,8 times, 4,3±1,9 times, 2,6±1,1 times) for the ages of 4, 5, 6. The relationship between age with CI and DO were not significant (P>0,05), but the relationship between age with S/C was significant (P<0,05). The value of S/C increased on dairy cattle at age of 4 to 5 years and started to decreased at the age of 6 years.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Wahyu Retno Swari; Emy Koestanti Sabdoningrum; Wurlina Wurlina; Suherni Susilowati; Rochmah Kurnijasanti; Erma Safitri
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.

DAMPAK PEMBERIAN ROTI AFKIR TERHADAP EFISIENSI REPRODUKSI SAPI PERAH Friesian Holstein DI WILAYAH KERJA KPSP SETIA KAWAN NONGKOJAJAR, PASURUAN Ricko Nurdianto S.; Sri Hidanah; Sri Pantja Madyawati; Abdul Samik; wurlina wurlina; Tri Wahyu Suprayogi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this research is to know the impact of bread waste to efficiency reproduction of Friesian Holstein cow in regional working KPSP Setia Kawan Nongkojajar, Pasuruan by value Service per Conception (S/C), Conception Rate (CR), Days Open (DO). Method in this research is the dependent variable observed is the reproductive efficiency, the dependent variable as a supporter is the cow has given birth, the healthy dairy cow, the feed volume given. The data obtained is processed by using descriptive analysis to get the reproduction efficiency figure. The result of the research indicate that the value of Service per Conception (S/C) = 1.5 ± 0.69 times, Days Open (DO) = 95.6 ± 11.32 days and Conception Rate (CR) = 65% and Fertility Status (FS) = 72,4%. Results of this study can be concluded that bread waste provided by the breeder as an additional feed for dairy cow has no significant effect.

Co-Authors Abdul Samik Abdullah Abdullah Arda Wahyu Setyadi Benjamin Christoffel Tehupuring Budiarto Budiarto Emy Koestanti Sabdoningrum Emy Koestanty Sabdoningrum ERMA SAFITRI Faradillah Hapsari Dwi Putri Hani Plumeriastuti Hanifah Muslimah Ananda Hastini Suryaningsih Herry Agoes Hermadi Koesnoto Supranianondo Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Meles, Dewa Ketut Mufasirin Nenny Harijani Nove Hidajati Nunuk Dyah Retno Lastuti Nur Zaini Nanda Mardian Oky Setyo Widodo Paraswita Eindah Fitri Pudji Srianto Retno Sri Wahjuni Ricko Nurdianto S. Rimayanti Rimayanti Rochmah Kurnijasanti Roesno Darsono Silvia Rani Andriyanti Siti Nur Fatimah Soeharsono Soeharsono Sri Chusniati Sri Hidanah Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Suzanita Utama Tatik Hernawati Tita Damayanti Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Vina Ari Prastyorini Wahyu Retno Swari Winadya Reika Ummaisyah

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