Roesno Darsono
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HUBUNGAN ANTARA BODY CONDITION SCORE (BCS) DENGAN PRODUKSI SUSU SAPI PERAH FRIESIAN HOLSTEIN (FH) Melsa Netika; Roesno Darsono; Budi Utomo; Imam Mustofa; Ismudiono Ismudiono; Tri Wahyu Suprayogi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.995 KB) | DOI: 10.20473/ovz.v8i2.2019.89-93

Abstract

The aim of this research was to observe the Body Condition Score (BCS) and milk production of Friesian Holstein (FH) dairy cattles. 144 of dairy cattles divided into 3 groups based on BCS 1-9 scale. The results showed that Y = 6.62 + 5.54X - 5.78X2. Coefficient of determination (R2) of 20.6 this mean any increase in milk production by 20.6% is affected by BCS. It can be concluded that there is a correlation between Body Condition Score (BCS) and milk production. The higher BCS (BCS 7-8) the lower milk production, while in ideal BCS production (5-6) the production of milk is optimize.
PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP KETEBALAN ENDOMETRIUM MENCIT (Mus musculus) Dyah Ayu Roro Risna Y; Sri Mulyati; Roesno Darsono; Imam Mustofa; Arimbi Arimbi; Widjiati Widjiati
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.027 KB) | DOI: 10.20473/ovz.v7i2.2018.120-125

Abstract

The purpose of the research was to know  the effect of Insulin-Like Growth Factor-I (IGFI) derived from pregnant crossbreed mare serum (PMS) on endometrium thickness of mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were P0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include histopatological endometrium thickness of mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The data was also be analyzed using General Linear Model Univarieted to see the comparison between IGF-I PMS and recombinant mouse. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on endometrium thickness of mice . It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the endometrium thickness of mice.
UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN Silvia Rani Andriyanti; Mas’ud Hariadi; Roesno Darsono; Pudji Srianto; wurlina wurlina; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.453 KB) | DOI: 10.20473/ovz.v7i2.2018.156-159

Abstract

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.
PROFIL GEN GROWTH HORMONE (GH) SAPI HASIL PERSILANGAN MADURA DAN LIMOUSIN DENGAN METODE PCR-RFLP Ghea Aquatica Puteri; Budi Utomo S; Roesno Darsono
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.32 KB) | DOI: 10.20473/ovz.v8i1.2019.43-48

Abstract

The purpose of this research was to find out the Growth Hormone (GH) gene profile of the cross breeding between Madura cattle and Limousin cattle (Madrasin). Sampl in the form of cattle blood for this research was obtained from 14 Madrasin cattles in the area of Bangkalan, Madura, East Java. DNA extraction was performed then to provide the result for PCR RFLP, which then indicated that Madrasin cattle’s GH gene profile has 432 base pair fragment length and the RFLP result indicated that Madrasin cattle’s GH genetic was cut off into 180 base pair, 250 base pair, 300 base pair, and 400 base pair. Moreover, there was no V genetic to be found in GH genetic of Madura cattle.