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All Journal Journal Of Vocational Health Studies Jurnal Medik Veteriner JPPI (Jurnal Penelitian Pendidikan Indonesia) Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary
Sri Pantja Madyawati
Depatemen Reproduksi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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STEM CELL THERAPY ON ANIMAL MODEL (Rattus norvegicus) END GESTATIONAL TIME EXPOSED TO CARBON BLACK AS OBSERVED FROM CASPASE-3 EXPRESSION Agung Budianto Achmad; Sri Pantja Madyawati; Widjiati Widjiati
Journal of Vocational Health Studies Vol. 1 No. 3 (2018): March 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I3.2018.102-106

Abstract

Background: Air pollution in the form of Diesel Exhaust Particles emerging from motor vehicles are harmful to health that have adverse reproductive health impacts, especially during pregnancy. The use of stem cells in treating white mice (Rattus norvegicus) exposed to carbon black can reveal the potential for treatment of placental impairment during pregnancy. Purpose: to demonstrate the effectivity of Rat Bone Marrow Mesenchymal Stem Cell therapy on rats (Rattus norvegicus) exposed to carbon black as observed from caspase-3 expression. Methods: This research uses a completely randomized design with factorial pattern. Forty-eight gravid female rats were divided into six treatment groups. Result: caspase-3 expression in each treatment showed no significant differences in the groups treated with RBMMSC in each gravid groups treatment (therapy GD 11 and GD 17) were exposed to carbon black and not treated with RBMMSC. The same are indicated by the normal trophoblast cells (cytotrophoblast and syncytiotrophoblast cells) in the RBMMSC treated group showed no significant difference with the group exposed to carbon black only. Conclusion: this research indicate that Rat Bone Marrow Mesenchymal Stem Cell therapy in Rattus norvegicus exposed to carbon black have not been able to reduce expression of caspase-3.
The Incidence Rate of Helminthiasis in The Digestive Tract of Dairy Cattle in The Partnership of PT. Greenfields Indonesia Wenny Nur Azizah; Dony Chrismanto; Sri Pantja Madyawati; Hana Eliyani
Journal of Applied Veterinary Science And Technology Vol. 1 No. 1 (2020): April 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I1.2020.11-15

Abstract

Background: One of the partnerships of PT. Greenfields Indonesia is located on the slopes of Mount Kawi, precisely in Pijiombo, Ngadirenggo Village, Wlingi District, Blitar Regency. The tropical situation in this area is very humid and has high rainfall. It causes dairy cattle to be infected with helminths. Therefore, observations of the incidence rate of gastrointestinal helminthiasis in dairy cattle are needed. Purpose: To determine the prevalence of gastrointestinal helminths in dairy cattle in the partnership of PT. Greenfields Indonesia. Method: Fecal samples from 36 dairy cattle were collected and examined using native, sedimentation, and flotation techniques. Result: Indicated that the prevalence of gastrointestinal helminths in the dairy cattle was about 66,66%. There were 24 dairy cattle positively and 12 cattle negatively. Helminth species found included class Nematoda (Haemonchus contortus, Oesophagostomum radiatum, Bunostomum phlebotomum, Mecistocirrus digitatus, and Gaigeria pachyscelis) and Cestoda (Moniezia expansa). Conclusion: The incidence rate of helminthiasis was quite high dairy cattle in the partnership of PT. Greenfields Indonesia.
Perbandingan Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Terhadap Persentase Motilitas, Viabilitas dan Integritas Membran Plasma Spermatozoa Kambing Sapera pada Penyimpanan Suhu 5ºC Novia Nur Iskandari; Sri Pantja Madyawati; Prima Ayu Wibawati; Tri Wahyu Suprayogi; Ragil Angga Prastiya; Bodhi Agustono
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.196-202

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan pengencer tris kuning telur dan susu skim kuning telur terhadap persentase motilitas, viabilitas dan integritas membran plasma spermatozoa kambing Sapera. Metode penelitian dengan mengumpulkan semen, pemeriksaan kelayakan semen segar, pengenceran semen, penyimpanan suhu 5˚C dan pemeriksaan setiap 24 jam. Data diolah menggunakan anova faktorial dengan membandingkan antara dua bahan pengencer terhadap waktu pemeriksaan. Data menunjukkan tris kuning telur signifikan (p<0.05) terhadap susu skim kuning telur. Lama penyimpanan menunjukkan hasil signifikan pada hari ke-1 aspek motilitas 84.44% : 80%, viabilitas 89.67% : 86% dan integritas membran plasma 83.67% : 79.33%. Tris kuning telur dan susu skim kuning telur menunjukkan adanya interaksi (p<0.05 ) terhadap waktu pemeriksaan motilitas namun tidak terdapat interaksi (p>0.05) terhadap waktu pemeriksaan viabilitas dan integritas membran plasma. Disimpulkan pengencer tris kuning telur dan susu skim kuning telur berpengaruh pada persentase motilitas, viabilitas dan integritas membran plasma spermatozoa.
Kualitas post-thawing semen domba Merino dalam bahan pengencer berbasis susu skim-kuning telur yang ditambah isolat crude protein Tirosine Kinase Krisna Widiantoro; Sri Pantja Madyawati; Trilas Sardjito; Tatik Hernawati; Indah Norma Triana; Sunaryo Hadi Warsito
Ovozoa : Journal of Animal Reproduction Vol. 10 No. 2 (2021): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i2.2021.39-45

Abstract

This study was conducted to determine the effect of crude protein tyrosine kinase (PTK) isolates supplementation into skim milk-egg yolk based diluent to maintain the quality of Merino ram spermatozoa.  Four ejaculates of two Merino rams were divided into two groups for the control group (P0): Merino ram semen was diluted in skimmed milk-egg yolk based diluent, and the treatment group (P1): Merino ram semen was diluted in skim milk-egg yolk based diluent contained crude PTK 1,597 mg/ml diluent. All diluted semen was equilibrated for 2 hours at 5 °C and filled into 0.25 mL French straws. The filled straws were placed on steel racks (Cooltop, Minitube) held in liquid nitrogen vapour for 10 minutes at –140 °C, immersed immediately in liquid nitrogen at –196 °C, and stored for 48 hours for  later assessment. Post-thawed semen samples were evaluated for spermatozoa motility, viability, and morphological abnormality. The results showed that the spermatozoa motility of fresh semen of Merino ram was 82.5 ± 2.89, which was qualified for freezing. The post-thawing spermatozoa motility, viability, and morphological abnormalities of Merino ram in the P1 group were 34.11 ± 3.26%, 38.00 ± 3.00%, and 12.89 ± 4.54%, respectively. It were higher (p <0.05) than the control group of 24.44 ± 2.9%, 26.67 ± 3.32%, and 21.11 ± 3.02%. It was concluded that the addition of crude PTK isolates of 1.597 mg/ml skim milk-egg yolk diluent improved the quality of post-thawed spermatozoa of Merino ram.
Evaluation of Production Peak of Laying Hens Strain Lohman Brown in CV. Lawu Farm Malang Yolifia Rahma Milenia; Sri Pantja Madyawati; Agung Budianto Achmad; Ratna Damayanti
Journal of Applied Veterinary Science And Technology Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V3.I1.2022.12-17

Abstract

Background: The productivity of laying hens is a key measurement in the laying hens business. One of the targets for laying hens is to achieve peak production. The optimal peak production period is necessary to attain the maximum profit. Purpose: To determine the productivity of laying hens strain Lohman Brown aged 25-49 weeks at CV. Lawu Farm Malang Regency as an evaluation of the peak production period. Case Analysis: The descriptive method with the case study technique was used. Primary data were obtained from observations by participating in direct livestock activities, interviews, and documentation, and secondary data were obtained from literature studies. The variables observed in this study were Hen Day Production, Feed Conversion Ratio, egg weight, and mortality. Results: Observation of laying hens productivity in CV. Lawu Farm showed that 71.42% of Hen Day Production value of the full cages had met the target with HDP of more than 92%, while 28.58% did not reach the peak production target by only getting HDP less than 90%. The FCR value was 2.4 – 2.15, the average egg weight wais 63.03 grams/egg, and the mortality was ≤3%. Conclusion: The productivity of laying hens of the Lohman Brown strain aged 24-59 weeks at CV. Lawu Farm has met production standards in terms of HDP, FCR, egg weight, and mortality.
Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing Herlina Masyitoh; Tri Wahyu Suprayogi; Ratih Novita Praja; Pudji Srianto; Sri Pantja Madyawati; Amung Logam Saputro
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.557 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.105-112

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan persentase motilitas dan viabilitas semen kambing Sapera terhadap evaluasi semen segar dan before freezing dengan penambahan pengencer tris kuning telur dan susu skim kuning telur. Metode penelitian ini adalah pengambilan semen, evaluasi semen segar (pemeriksaan makroskopis dan mikroskopis), pengenceran semen, gliserolisasi, ekuilibrasi dan evaluasi before freezing. Penelitian ini terdiri dari P0, P1 dan P2, masing-masing perlakuan terdiri dari sembilan ulangan. P0 adalah semen segar kambing Sapera, P1 adalah semen kambing Sapera+pengencer tris kuning telur dan P2 adalah semen kambing Sapera+pengencer susu skim kuning telur. Data yang diperoleh dengan Uji T2 sampel dependent, membandingkan P0 dan P1 serta P0 dan P2. Data menunjukkan perbedaan sangat nyata (p<0,01) pada perlakuan P0 dan P1 persentase motilitas 76,11%; 65,56% dan viabilitas 82,33%;72,22%. P0 dan P2 persentase motilitas 76,11%;61,11% dan viabilitas 82,33%;67,78%. Hasil tersebut menunjukkan bahwa penelitian persentase motilitas dan viabilitas spermatozoa kambing Sapera mengalami penurunan dari semen segar pada tris kuning telur dan susu skim kuning telur before freezing.
Efektifitas Daya Pisah Electric Separating Sperm (ESS) terhadap Spermatozoa Kromosom X dan Y pada Kambing Sapera Fahlevi Lailiyah; Pudji Srianto; Amung Logam Saputro; Sri Pantja Madyawati; Bodhi Agustono; Ragil Angga Prastiya
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.223 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.93-98

Abstract

Penelitian ini bertujuan untuk mengetahui efektifitas pemisahan alat Electric Separating Sperm (ESS) terhadap spermatozoa kromosom X dan Y pada sisi anoda dan katoda dengan variabel meliputi presentase viabilitas, motilitas, abnormalitas dan morfometri spermatozoa. Metode pengambilan semen menggunakan vagina buatan, kemudian dilakukan pemeriksaan makroskopis dan mikroskopis, apabila menunjukkan hasil yang baik dilanjutkan dengan pengenceran semen dengan Tris Kuning Telur dan dilakukan pemisahan. Terdapat tiga perlakuan pada penelitian ini yaitu P1 (3 menit), P2 (7 menit), P3 (10 menit) dengan enam kali pengulangan pada sisi anoda dan katoda. Data pada tiap variabel (viabilitas, motilitas dan abnormalitas) diperiksa menggunakan analisis statistik ANOVA dan untuk melihat adanya pemisahan spermatozoa kromosom X dan Y dilakukan pengukuran terhadap kepala menggunakan morfometri. Hasil pemisahan tertinggi yaitu pada P3 dengan perbandingan pemisahan X : Y sejumlah 65,00:35,00 pada sisi katoda, kemudian pada sisi anoda persentase perbandingan X : Y yaitu 59,00:41,00. Hasil kualitas spermatozoa setelah pemisahahn menunjukkan presentase viabilitas terbaik terdapat pada P1 yaitu 75,00a±2,00 pada sisi anoda dan 76,50a±2,17 pada sisi katoda. Motilitas spermatozoa P1 menunjukkan motilitas progresif spermatozoa dengan rataan 60,83a±2,04 pada anoda dan 61,67a±2,58 pada sisi katoda. Presentase abnormalitas tidak menunjukkan perbedaan yang signifikan antar perlakuan.
Produksi dan Kualitas Telur Itik Alabio di Daerah Sentra Peternakan Desa Sungai Pandan, Kabupaten Hulu Sungai Utara, Kalimantan Selatan Rini Fajarwati; Sarmanu Sarmanu; Chairul Anwar Nidom; Sri Pantja Madyawati; Imam Mustofa; Mirni Lamid; Sri Hidanah; Widya Paramita Lokapirnasari; Tarzan Purnomo; Mohammad Sukmanadi
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.246-250

Abstract

Penelitian ini merupakan penelitian deskriptif dengan menggunakan 20 itik Alabio betina siap bertelur dengan rentang usia 6 bulan dan untuk pemeriksaan kualitas telur untuk masing-masing kelompok 10 sampel. Pengamatan tingkat produksi telur dilakukan selama tiga bulan dengan 10 pengulangan, kualitas telur (ketebalan kulit telur, persentase kulit telur, persentase kulit telur, persentase albumin, persentase kuning telur, huagh unit, warna kuning telur, kadar protein dan lemak kandungan). Data produksi telur dan kualitas telur diuji menggunakan uji Anova. Hasil pengamatan rata-rata produksi harian telur 15.45 ± 0.12, 14.72 ± 0.10, 14.91 ± 0.09; produksi berat harian (kg/hari) 925.27 ± 7.50, 875.40 ± 6.32, 884 ± 5.03; hasil dari ketebalan kulit telur kualitas 0.33 ± 0.01, 0.33 ± 0.12, 0.33 ± 0.12; persentase kulit telur 11.32 ± 0.93, 11.28 ± 0.10, 11.36 ± 0.05; persentase albumin 55.70 ± 1.08, 56.66 ± 1.22, 56.12 ± 1.00; persen kuning telur 32.07 ± 1.24, 31.55 ± 1.48, 31.64 ± 1.20; Haugh unit 88.70 ± 6.21, 91.41 ± 6.70, 94.51 ± 5.06. Warna kuning masing-masing memiliki skor 15. Dapat disimpulkan bahwa produksi dan kualitas telur itik Alabio baik.
The Steroid Hormone Profile in Etawah Crossbreed Goat While Ovulation Induced using The Selectsynch Method Muhammad Aftabuddin Rz; Pudji Srianto; Chairul Anwar Nidom; Sri Pantja Madyawati; Trilas Sardjito; Ismudiono Ismudiono
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.62-68

Abstract

This study aimed to provide alternative information and solutions in an effort to increase reproductive productivity in etawah crossbreed goats (PE). The sample used in this study was 10 female PE with an average age of 2.5 - 3 years and primiparous at least . Ovulation induction was performed using 0.1 mg intra-muscular Gonadotropin-Releasing Hormone (GnRH), after seven days injection of Prostaglandin F2α (PGF2α) was given as much as 2.5 mg submucosa of the vulva, followed by a second injection of GnRH as much as 0,2 mg intramuscular in samples that have really experienced heat and selected selectively according to the signs of natural heat shown by the sample and followed by insemination as much as 2 doses or 0.50 ml of frozen semen. Blood sampling was performed at H0, H7th, H14th and H21th after insemination. All blood samples were collected and progesterone and estrogen hormone profiles were examined using the ELISA method. From the results of the ELISA test, the mean progesterone hormone profile H0 = 4.798 ng / ml, H7th = 4.887 ng / ml, H14th = 4.824 ng / ml, H21th = 5.148 ng / ml. The profile of the hormone estrogen at H0 = 19,461 pg / ml, H7th = 17,457 pg / ml, H14th = 18,248 pg / ml, H21th = 17,515 pg / ml. This study showed an increase in the levels of the progesterone hormone at H0 to H7th, then slightly decreased in H14th and a significant increase in H21th. For the estrogen hormone, there is a decrease from H0 to H7th then there is a slight increase in H14th and decreases again in H21th.
Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction Ivo Febrina Prasetyo; Budi Utomo; Sri Pantja Madyawati
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.125-130

Abstract

The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR.
Co-Authors Adinda Riezki Irawan Agung Budianto Achmad Amung Logam Saputro Benjamin Christoffel Tehupuring Bodhi Agustono Budi Utomo Chairul Anwar Nidom Chusniati, Sri Dito Herrys Dika Putra Dony Chrismanto Emy Koestanti Sabdoningrum Fahlevi Lailiyah Fendy Suhariadi Hana Eliyani Herlina Masyitoh I. Ismudiono Imam Mustofa Indah Norma Triana Ivo Febrina Prasetyo Jihan Pangestuningrum Krisna Widiantoro Mirni Lamid Mohammad Sukmanadi Muhammad Aftabuddin Rz Novia Nur Iskandari Prima Ayu Wibawati Pudji Srianto Ragil Angga Prastiya Ratih Novita Praja Ratna Damayanti Rini Fajarwati Rochmi, Siti Eliana Sardjito, Trilas Sarmanu Sarmanu Sri Hidanah Sri Hidanah TARZAN PURNOMO Tatik Hernawati Tri Wahyu Suprayogi Warsito, Sunaryo Hadi Wenny Nur Azizah Widjiati Widjiati Widya Paramita Lokapirnasari Yolifia Rahma Milenia