Ratna Stia Dewi
Fakultas Biologi, Universitas Jenderal Soedirman Jl. dr. Suparno, Karangwangkal, Purwokerto 53123

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Decolorization Of Indigosol Blue Dye Using Trametes versicolor F200 and Aspergillus sp Sunu Pertiwi; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.848 KB) | DOI: 10.20884/1.bioe.2020.2.2.1835

Abstract

The dyeing process of batik eventually produces much of wastewater. The difficult degradation and the dangers posed within the synthetic dyes are the main concerns in finding efficient wastewater treatment. Biological treatment has been known to be an effective technique of reducing or eliminating color intensity in wastewater. Fungi is one organism that can decompose many environmental pollutants. The aims of this research were to determine the ability of fungal isolates in decolorizing the synthetic dyes and analyzed which treatment has the highest decolorization percentage. Fungal isolates of Trametes versicolor F200 and Aspergillus sp. were used as a biological agent to decolorize of Indigosol Blue dye. The decolorization percentage was analyzed by spectrophotometer method. The result showed that T. versicolor F200 and Aspergillus sp. able to decolorize Indigosol Blue dye. The decolorization treatment of Indigosol Blue dyes using T. versicolor F200 showed the highest decolorization percentage reaching 97.21%.
OPTIMASI WAKTU INKUBASI DAN pH Ganoderma sp. DARI KEBUN RAYA BATURRADEN UNTUK DEKOLORISASI RBBR Maria Pricilia Gita; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.947 KB) | DOI: 10.20884/1.bioe.2020.2.1.1771

Abstract

Jamur pelapuk putih diketahui memiliki kemampuan untuk mendekolorisasi pewarna, salah satunya jamur Ganoderma sp. dari Kebun Raya Baturraden. Jamur tersebut mampu mendekolorisasi Remazol Brillliant Blue R (RBBR) yang bersifat toksik, mutagenik, karsinogenik, dan stabil terhadap perlakuan fisika maupun kimia. Proses dekolorisasi dapat dipengaruhi oleh faktor lingkungan seperti waktu inkubasi dan pH. Setiap jamur pelapuk putih memiliki waktu inkubasi dan pH optimum yang berbeda dalam mendekolorisasi pewarna. Penelitian ini bertujuan untuk mengetahui kemampuan isolat Ganoderma sp. dari Kebun Raya Baturraden dengan waktu inkubasi dan pH berbeda dalam mendekolorisasi pewarna RBBR, serta mengetahui variasi waktu inkubasi dan pH yang menunjukkan hasil terbaik. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) dengan Perlakuan variasi pada penelitian ini terdiri dari waktu inkubasi yaitu 24, 48, 72, 96, dan 120 jam, serta pH 3, 4, 5, 6, dan 7. Hasil penelitian menunjukkan bahwa Ganoderma sp. dari Kebun Raya Baturraden mampu mendekolorisasi RBBR pada waktu inkubasi dan pH berbeda dengan persentase dekolorisasi 4,10% - 83,04%. Persentase dekolorisasi tertinggi ditunjukkan pada waktu inkubasi 96 jam dan pH 6, yaitu 83,04%. Hal tersebut membuktikan bahwa Ganoderma sp. dari Kebun Raya Baturraden memiliki waktu inkubasi optimum 96 jam, serta pH optimum 6 untuk mendekolorisasi RBBR.
Aspergillus sp. For Indigosol Blue and Remazol Brilliant Blue R Decolorization Fitria Ayudi Ulfimaturahmah; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.1795

Abstract

Synthetic dyes are artificial dyes manufactured by Industry and commonly used for the textile industry. These dyes had potentially caused an environmental problem. Many types of dyes are recalcitrant and have toxic properties for living organisms. It can be removed by decolorization method, especially a biological decolorization by fungi. Fungi were chosen due to the ability to degrade toxic components. Aspergillus sp. is the fungi which commonly used for dye decolorization. It might be caused that Aspergillus sp. is one type of fungi lived in the textile waste and expected not to die in the dye decolorization treatment. The purpose of this research was to investigate the ability of the mycelia pellets of Aspergillus sp to decolorized Indigosol Blue dye and Remazol Brilliant Blue R (RBBR) dye. This research showed that mycelial pellets of Aspergillus sp. had high activity of decolorization of Indigosol Blue dye up to 85.37% and RBBR dye up to 80.21% and caused low pH value after 24 hour incubation time compared to the control solution.
Aktivitas Enzimatik Isolat Jamur Auricularia sp., Trametes sp., dan Pholiota sp. pada Pewarna Remazol Brilliant Blue R dengan Variasi pH Agung Wiriat Putra Pratama Hadi; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.325 KB) | DOI: 10.20884/1.bioe.2020.2.1.1772

Abstract

Limbah dari industri tekstil berupa zat warna sintetik seperti Remazol Brilliant Blue R (RBBR) dapat menimbulkan pencemaran lingkungan akibat sifat zat warna RBBR yang toksik dan sulit terurai. Isolat Auricularia sp.,Trametes sp., dan Pholiota sp. memiliki enzim yang mampu mendekolorisasi komponen pewarna RBBR. Aktivitas enzim lignolitik seperti lakase yang dimiliki jamur mampu mengoksidasi pewarna tekstil. Aktivitas enzim lakase dipengaruhi oleh jenis jamur yang digunakan, pH, suhu, dan waktu inkubasi. Penelitian bertujuan untuk mengetahui aktivitas enzim lakase dari isolat jamur Auricularia sp., Trametes sp., dan Pholiota sp. dalam pewarna RBBR pada nilai pH berbeda, serta mengetahui isolat pada pH optimum yang memiliki aktivitas enzim lakase dalam pewarna RBBR. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Variabel bebas yang diamati adalah jenis isolat dan kondisi pH (4, 8, dan 12), sedangkan variabel tergantungnya adalah kemampuan isolat dalam menghasilkan enzim. Parameter utama yang diamati adalah aktivitas enzim Lakase. Parameter pendukung terdiri atas nilai bobot kering miselium. Hasil menunjukan ketiga isolat memiliki aktivitas enzim lakase dalam RBBR dengan pH berbeda. Aktivitas tertinggi terdapat pada Trametes sp. dalam RBBR dengan pH 4 yaitu sebesar 101,9 U/mL. Aktivitas terendah terdapat pada Aricularia sp. dalam RBBR dengan pH 4 sebesar 48,6 U/mL.
Isolasi dan Identifikasi Jamur Endofit Tanaman Manggis (Garcinia mangostana L.) Iva Akmalasari; Endang Sri Purwati; Ratna Stia Dewi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.131

Abstract

Mangosteen (Garcinia mangostana L.) is one of Indonesia's tropical horticultural commodities with high economic value. Mangosteen also has a lot of functions such as a producer of a wide variety of functional compounds such as anticancer, antiviral, antibacterial and antifungal that have some bioactive component i.e., micotoxin, enzyme, and antibiotics. The research was carried out to investigate the endophyte fungi in mangosteen plant tissue. Endophyte fungal isolation from mangosteen plant tissue resultsed 11 isolates which have identified Phoma sp., Acremonium sp., 2 isolates of Penicillium sp., Geotrichum sp., Pestalotiopsis sp., Botryosphaeria sp., Colletotrichum sp., Chrysosporium sp., Aspergillus sp. and Blastomyces sp., and 2 isolates have not been identified.
Dekolorisasi Limbah Cair Batik Menggunakan Miselium Jamur yang Diisolasi dari Limbah Baglog Pleurotus ostreatus Anna Yulita; Sri Lestari; Ratna Stia Dewi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.132

Abstract

Batik industries have negative impacts on the environment from the dye used. An alternative method for decolorization in batik wastewater using the fungi Pleurotus ostreatus was grown in baglog. Spent mushroom (baglog) still contains fungal mycelium of P. ostreatus and contaminant fungus such as Penicillium sp., and Aspergillus sp.). Those fungi have a potential to decoloring dyes in batik wastewater. The aim of this research was to determine the ability of fungal mycelium isolated from spent mushroom P. ostreatus at decoloring batik wastewater, and determine the most effective fungal mycelium to decoloring batik wastewater. The results showed that the mycelium isolated from sewage baglog P. ostreatus was able to decoloring batik wastewater. Penicillium sp. mycelium is most effective at decoloring batik wastewater.
Biodegradasi Bioplastik Berbasis Pati Menggunakan Isolat Fungi Indigenous Asal Tempat Pembuangan Akhir Gunung Tugel, Kabupaten Banyumas Jimmy Al Fa'is; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4605

Abstract

The use of plastic in Indonesia, especially as packaging, is very popular among the public, because it is practical and has many uses. Plastic consumption in 2020 even reached 67.8 million tons per day. Therefore, one way to overcome this problem is to use and produce biodegradable plastics or bioplastics. Biodegradation of bioplastics can be done with fungi. The potential place to find bioplastic degrading fungi is the Gunung Tugel Ex-Final Disposal Site (TPA). Considering the fact that bioplastiks have a fairly good level of degradation, in this study a starch-based biodegradation test will be carried out using isolats of indigenous fungi originating from Ex-TPA Gunung Tugel using the method of calculating the weight loss of bioplastics (weight loss). This study aims to obtain starch-based bioplastic degrading fungi isolats from Ex-TPA Gunung Tugel, Banyumas Regency and determine the highest biodegradability ability of starch-based bioplastics by fungal isolats from Ex-TPA Gunung Tugel, Banyumas Regency. The results showed that isolats of indigenous fungi that had the potential to degrade starch-based bioplastics from Ex-TPA Gunung Tugel, Banyumas Regency were obtained from the genus Aspergillus (GT2 isolate) and Penicillium (GT1, GT3, GT4, GT5, and GT6) isolate. The highest biodegradability of starch-based bioplastics was found by fungi from the genus Aspergillus, namely isolate GT2 with a weight loss of 21.84%. The biodegradation occurs due to the activity of endoamylase, exoamylase, and glucanase enzymes produced by fungi. The observation of the texture of the bioplastic resulted in a significant change in color, namely from the white bioplastic sheet to brownish. These observations also showed the attachment of fungal mycelium on the surface of the bioplastik accompanied by a hollow and uneven surface of the bioplastikc. Keywords : Biodegradation, Starch-based bioplastik, Indigenous Fungi, TPA.
Dekolorisasi Limbah Batik Menggunakan Jamur Aspergillus sp. 3 Teramobil Bentonit pada Waktu Inkubasi Berbeda Kholilatul Kamalia; Ratna Stia Dewi; Mardiyah Kurniasih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 2 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.2.5181

Abstract

Industri batik menghasilkan air limbah dengan kandungan zat warna yang tinggi. Salah satu usaha industri batik yang berkembang baik, terdapat di Sokaraja Banyumas. Metode yang potensial untuk pengolahan limbah batik yaitu dengan memanfaatkan jamur indigenous. Aspergillus sp. 3 dapat digunakan untuk dekolorisasi limbah batik Indigosol Blue. Dekolorisasi limbah batik dapat menggunakan teknik amobilisasi atau perlekatan pada substrat. Bentonit merupakan jenis mineral lempung yang memiliki potensi sebagai support material amobilisasi. Penelitian ini bertujuan untuk mengetahui kemampuan jamur Aspergillus sp. 3 yang teramobil bentonit dengan lama waktu inkubasi berbeda terhadap dekolorisasi limbah batik, dan mengetahui waktu lama inkubasi yang efektif pada penggunaan jamur Aspergillus sp. 3 yang teramobil bentonit terhadap dekolorisasi limbah batik. Penelitian ini menggunakan metode eksperimental Rancangan Acak Lengkap (RAL) dengan perlakuan waktu inkubasi 0, 6, 12, 18, 24, 30, 36 jam. Variabel bebas yaitu waktu inkubasi, sedangkan variabel terikat yaitu kemampuan jamur Aspergillus sp. 3 yang teramobil bentonit dalam dekolorisasi limbah batik. Parameter utama berupa persentase dekolorisasi limbah batik IB, sedangkan parameter pendukung adalah pH, suhu, TDS, dan pemakaian berulang pelet miselium teramobil bentonit. Data persentase dekolorisasi dianalisis menggunakan analisis ragam pada tingkat kesalahan 5% dilanjutkan uji Beda Nyata Lanjut dengan tingkat kesalahan 5%. Waktu inkubasi 6 jam merupakan waktu yang efektif bagi Aspergillus sp. 3 teramobil bentonit untuk mendekolorisasi limbah batik IB sebesar 97,43%, pH berkisar 5,40-7,30, suhu berkisar 30-31 ÂșC, persentase TDS berkisar 14,63-40,31, dan pemakaian berulang pelet miselium teramobil bentonit sampai 3X.
Optimasi Pertumbuhan Miselium Jamur Trametes sp. pada Media Membran Dekolorisasi Dengan Komposisi Bahan Organik yang Berbeda Salma Aulia Rahma; Ratna Stia Dewi; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 1 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4648

Abstract

White rot fungus species such as Trametes sp. have been known to able to decolorize azo dyes, heterocyclics, and reactive or polymeric compounds through the degradation of ligninolytic enzymes. Decolorization membrane is a medium that used as a means to reduce waste, one of which is batik waste. The decolorization membrane is made in a certain form by functioning as a growth medium for fungal mycelium which is used as an alternative in bioreactors. The main ingredient for making decolorizing membranes is sawdust. Other materials needed are supplements such as lime (CaCO3), gypsum (CaSO4), and water. This study used an experimental method with a completely randomized design (CRD), with 5 treatments and each with 5 replications. The results showed that differences in the composition of the organic matter media affected the growth of the mycelium of the fungus Trametes sp. Differences in the composition of each treatment showed significant results. Further test data showed that the composition of P3 was the optimum membrane medium for the mycelium growth of the fungus Trametes sp. with a composition of 65% sawdust, 15% rice bran, 5% lime, 5% gypsum, and the addition of 10% milled corn. The results also show that the nutrients contained in the media can affect the growth of mycelium. The addition of the composition has a good effect on the growth of the mycelium as long as the composition is calculated correctly.
Optimasi Dosis Fungi Mikoriza Arbuskula (FMA) pada Tanah Lahan Bekas Tambang Batubara terhadap Tanaman Jagung (Zea mays) Dewi Yunita; Ratna Stia Dewi; Riza Zainuddin Ahmad
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 2 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.2.5455

Abstract

Pertambangan batubara di Indonesia umumnya dilakukan dengan sistem tambang terbuka sehingga menyebabkan pertumbuhan tanaman kurang optimal. Salah satu strategi untuk mengatasi hal tersebut adalah dengan penggunaan Fungi Mikoriza Arbuskula (FMA). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian inokulum FMA terhadap pertumbuhan tanaman jagung dan mengetahui dosis optimum FMA terhadap pertumbuhan tanaman jagung dengan media tanam tanah bekas lahan tambang. Penelitian ini menggunakan metode eksperimental dengan Rancangan Acak Lengkap (RAL) yaitu 4 dosis FMA (0, 100, 150, 200 g) pada medium tanah bekas tambang batubara dengan 3 kali ulangan. Variabel bebas yang digunakan yaitu dosis FMA dan variabel terikat yaitu kemampuan FMA dalam menginfeksi akar. Parameter utama yang diamati yaitu derajat infeksi akar, parameter pendukung yaitu bobot kering tanaman, jumlah daun dan tinggi tanaman jagung. Data dianalisa menggunakan uji Analisis Ragam (ANOVA) dengan standar kesalahan 5% dan dilanjutkan Beda Nyata Jujur (BNJ). Hasil penelitian menunjukkan bahwa perlakuan dosis FMA pada tanah lahan bekas tambang batubara memberikan pengaruh terhadap pertumbuhan tanaman jagung umur 42 hari setelah tanam (HST). FMA memberikan pengaruh positif terhadap pertumbuhan tanaman jagung. Dosis 100 g/polybag merupakan dosis efektif meningkatkan pertumbuhan tanaman jagung pada tanah bekas tambang batubara. Dosis 200 g/polybag memberikan hasil terbaik terhadap tinggi tanaman, jumlah daun dan bobot kering tanaman jagung.