Articles
<![CDATA[The prognostic value of the p53 gene expression and mutation in ovarian cancer ]]>
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Masadah, Rina]]>
<![CDATA[ Medical Journal of Indonesia Vol 18, No 2 (2009): April-June ]]>
Publisher : <![CDATA[Faculty of Medicine Universitas Indonesia ]]>
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DOI: 10.13181/mji.v18i2.345
<![CDATA[Aim This study aimed to observe the role of the p53 gene in ovarian carcinogenesis, and identify the prognostic significance of the expression of the p53 protein expression in ovarian cancer.Methods Fourty one ovarian cancer patients were examined in this study by analyzing the mutations of p53 gene from their blood samples, and by analyzing the p53 protein expression from ovarian cancer tissues.Results Mutational analysis in âhot spotâ exons (exons 5-9) and in ânon hot spot exonâ (exon 11) was performed by PCR-sequencing methods and p53 expression analysis was performed by immunohistochemistry method. Mutations of p53 were found in all samples, and the mutations were mostly found in âhot spotâ exons, though few mutations were also found in ânon hot spotâ exon. The expression levels of p53 protein were found higher in advanced stages of ovarian cancer. Six month to two year post surgery follow-up of patients showed that high expression of p53 protein was in line with high mortality rate.Conclusion This study showed that the p53 gene could be used as a prognostic indicator of ovarian cancer. (Med J Indones 2009; 18:81-90).Keywords: p53, ovarian cancer, prognostic significance   ]]>
<![CDATA[ Phosphatase Regenerating Liver3 and ECadherin Expression in Epithelial Ovarian Cancer ]]>
<![CDATA[Maharani, Risma]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Masadah, Rina]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 4, No.3, July 2016 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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<![CDATA[Objective: To determine the expression of Phosphatase Regenerating
Liver-3 (PRL-3) and E-Cadherin in the epithelial ovarian cancer
on various stages and differentiation grades.
Method: This was a cross-sectional study design conducted at
Obstetrics and Gynecology Department of several teaching hospitals,
Faculty of Medicine Universitas Hasanuddin from January
to June 2015. The expression of PRL-3 and E-cadherin was
assessed immunohistochemically in 40 patients with epithelial
ovarian cancer including 15 patients in early stage and 25 patients
in advanced stage. We used the Fisherâs exact test with the
significance of p0.05). The significant difference was
found in the expression of E-cadherin whereas the high expression
was shown at early stage than advanced stage (p0.05). This study also pointed out no
correlation between the expression of PRL-3 and E-cadherin in
epithelial ovarian cancer (p>0.05).
Conclusion: PRL-3 overexpression does not decrease E-cadherin
expression in epithelial ovarian cancer.
Keywords: E-cadherin, epithelial ovarian cancer, PRL-3]]>
<![CDATA[A1298C Polymorphism of Fetal Methylenetetrahydrofolate Reductase (MTHFR) Gene as a Risk Factor for Spontaneous Abortion ]]>
<![CDATA[Sulfiana, Isah]]>;
<![CDATA[T Chalid, St Maisuri]]>;
<![CDATA[Farid, Retno B]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Hartono, Eddy]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v4i2.77
<![CDATA[Objective: To investigate the role of A1298C polymorphism of fetal
methylenetetrahydrofolate reductase (MTHFR) gene in spontaneous
abortion.
Method: The case control study design recruited 96 subjects in Siti
Fatimah and Pertiwi mother and child hospital, Dr. Wahidin Sudirohusodo,
Pelamonia, Bhayangkara, Syekh Yusuf, Haji and Labuang
Baji hospital from March to September 2014. All subjects fulfilling
the inclusion criteria were taken tissue samples from mothers experiencing
spontaneous abortion and blood samples from normally
born baby. The data were analyzed using Pearson chi-square with
significant rate of 5% (p]]>
<![CDATA[Vascular Endothelial Growth FactorC Serum and Endostatin Serum as Predictors of Lympho Vascular Invasion in Early Stage Cervical Cancer ]]>
<![CDATA[Rusmardiani, Andhinna]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Lukas, Efendi]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 5, No. 2, April 2017 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v5i2.529
<![CDATA[Objective: To determine the relationship of Vascular Endothelial Growth Factors-C (VEGF-C), endostatin and the ratio of VEGF-C/ endostatin with limfo vascular invasion in patient with early stage cervical cancer.
Methods: This study used a cross sectional method. Samples were all patients with early stage cervical cancer who came to the several teaching hospitals of Obstetrics and Gynecology Department Universitas Hasanuddin Medical Faculty that meet the criteria,
then we measured the levels of VEGF-C and endostatin.
Results: We get 30 women with cervical carcinoma. The results showed that the serum levels of VEGF-C was higher in limfo vascular invasion positive (p = 0.017); the ratio of VEGF-C/ endostatin higher in limfo vascular invasion positive (p = 0.004); whereas serum levels of endostatin did not differ significantly in limfo vascular invasion positive or negative (p = 0.522).
Conclusion: The level of VEGF-C and VEGF-C/ endostatin ratio was higher in patients with early stage cervical cancer with positive LVSI than negative LVSI.
[Indones J Obstet Gynecol 2017; 5-2: 105-109]
Keywords: cervix uteri cancer, endostatin, limfo vascular invasion, VEGF-C]]>
<![CDATA[Sensitivity and Specificity of the SelfAdministered HPV Testing in Detecting Precancerous Conditions of the Cervix and Cervical Cancer ]]>
<![CDATA[Nyngsi, Erny M]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Moeljono, Eddy]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 5, No. 3, July 2017 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v5i3.547
<![CDATA[Objective: To determine the sensitivity and specificity of selfadministered Human Papilloma virus (HPV) test in detecting pre-cancerous lesions and high degree cervical cancer.
Methods: A case control study design was used in this study. This study was conducted at Dr. Wahidin Sudirohusodo Hospital and its affiliate hospitals during the period of October 2014 to May 2015. Measurement data using HPV self test grant from the Dutch School Netherlands, Evalyn Brush. Collecting data used questionnaires before and after doing HPV self test. Laboratory tests are carried out in Kalbe Genomic using HPV Genotyping capable detect 35 types of the HPV virus.
Results: A total of 101 subjects were involved in this study. The subjects were divided into two groups, the case and the controls (n = 50/51). Level of sensitivity and specificity were obtained for HPV self test (56% vs 98%). The level of acceptance of new tests by 62.37% (63 out of 101 respondents) of them admitted that the new test is simple / easy enough to do. The acuracy rate of diagnostic test of this examination is 79%.
Conclusion: Self-administered HPV test could be used as an alternative or primary screening for cervical cancer.
[Indones J Obstet Gynecol 2017; 5-3: 173-179]
Keywords: HPV, self, specificity, the sensitivity]]>
<![CDATA[Conformity of Human Papillomavirus between Self-examination of Vaginal Fluid and Cervical Specimen with Fluid-Based Cytology in Precancerous Lesions ]]>
<![CDATA[Anwar, Rahmawaty]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Moeljono, Eddy R.]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume 6 No. 4 October 2018 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v6i4.851
<![CDATA[Abstract
Objective: To determine the conformity of human papillomavirus between self-examination of vaginal specimen and cervical specimen with fluid-based cytology in precancerous lesions.
Methods: A cross-sectional study performed on cervical and vaginal fluid from 90 pre-cancerous lesions patients from April to September 2016. Cytological examination performed with self-examination and liquid-based cytology technique. HPV genotyping performed with PCR technique. Data were analyzed with SPSS.
Results: Most of the women aged >35 years (89%), 78% (71/90) multiparity and 74.4% (67/90) do not know about HPV screening. High risk type found in both vaginal and cervical fluid was type 16, 18, 33 and 45 whereas type 35 found only in vaginal fluid. The most prevalent high-risk HPV for both specimens were type 16 and 18. HPV type 42 and 53 were the low risk HPV found in the vaginal and cervical specimens (table 2). Cohen’s kappa for inter-test agreement shows a strong correlation (r=0.864).
Conclusion: The HPV self-examination method can be used as a primary examination of cervical cancer lesions detection in addition to fluid-based cytology with the similar results.
Keywords: Human papillomavirus, self-examination, fluid-based cytology, cervical cancer
Abstrak
Tujuan: Untuk mengetahui tingkat kesesuaian antara pemeriksaan HPV mandiri dari spesimen vagina dan hasil pemeriksaan sitologi berbasis cairan dari spesimen serviks.
Metode: Penelitian cross-sectional dilakukan pada cairan serviks dan vagina dari 90 pasien lesi pra-kanker pada April sampai September 2016. Pemeriksaan sitologi dilakukan dengan pemeriksaan diri dan teknik sitologi berbasis cairan. Pemeriksaan genotip HPV dilakukan dengan teknik PCR. Data dianalisis dengan SPSS.
Hasil: Sebagian besar wanita dalam penelitian iniaberusia >35 tahun (89%), 78% (71/90) multiparitas dan 74,4% (67/90) tidak mengetahui tentang skrining HPV. Tipe HPV risiko tinggi yang ditemukan pada cairan vagina dan serviks adalah tipe 16, 18, 33 dan 45 sedangkan tipe 35 hanya ditemukan pada cairan vagina. Tipe HPV risiko tinggi yang dominan untuk kedua spesimen adalah tipe 16 dan 18. HPV tipe 42 dan 53 adalah HPV risiko rendah yang ditemukan pada baik spesimen vagina maupun serviks. Kappa Cohen untuk tingkat kesesuaian antara pemeriksaan mandiri dan sitologi berbasis cairan menunjukkan korelasi kuat (r = 0,864).
Kesimpulan: Metode pemeriksaan HPV secara mandiri sendiri dapat digunakan sebagai pemeriksaan primer deteksi lesi kanker serviks selain sitologi berbasis cairan dengan hasil yang sama.]]>
<![CDATA[Human Papilloma Virus Selftesting as an Alternative Method for Cervical Cancer Screening ]]>
<![CDATA[Chandra, Richard]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Malinta, Umar]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 5, No. 2, April 2017 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v5i2.531
<![CDATA[Objective: To determine the level of acceptance of self Human Papilloma Virus (HPV) examination and to compare the level of sensitivity and specificity of self HPV examination with Fluid Based Cytology on precancerous cervical lesion and cervical cancer.
Methods: The analytical method used in this research was cross sectional with purposive sampling. This study is conducted at Wahidin Sudirohusodo Hospital and its affiliation from October 2014 to May 2015 with 101 subjects. The data measurement used self HPV examination tool and fluid based cytology kit. The data collected through questionnaires before and after the examination. The laboratory examination was carried out using the way of HPV Genotyping primary system (MY09/11) method Wl LTS-06, which is able to
detect 35 types of HPV viruses.
Results: A total of 101 subjects were involved in this study. The subjects were divided into two groups: the abnormal and normal group (n - 50/51). The acceptance level of self HPV examination was 62.37% (n = 63/101). Majority of subjects (88.21%) were ready to repeat the same examination in the future. The level of sensitivity and specificity for self HPV were (56% (95%CI (41.25 - 70.01%)) vs 98% (95%CI (89.55 - 99.95%)), p=<0.000001, while the level of sensitivity and specificity for fluid based cytology were (40% (95%CI (26.41 - 54.82%)) vs 98% (95%CI (89.35 - 99.95%)), p=0.000002.
Conclusion: HPV self-testing is very potential to be used as an alternative method for cervical cancer screening.
[Indones J Obstet Gynecol 2017; 5-2: 114-119]
Keywords: cervical cancer, cervical cancer screening, human papilloma virus, sensitivity, specificity]]>
<![CDATA[A1298C Polymorphism of Fetal Methylenetetrahydrofolate Reductase (MTHFR) Gene as a Risk Factor for Spontaneous Abortion ]]>
<![CDATA[Sulfiana, Isah]]>;
<![CDATA[T Chalid, St Maisuri]]>;
<![CDATA[Farid, Retno B]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Hartono, Eddy]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v4i2.77
<![CDATA[Objective: To investigate the role of A1298C polymorphism of fetal
methylenetetrahydrofolate reductase (MTHFR) gene in spontaneous
abortion.
Method: The case control study design recruited 96 subjects in Siti
Fatimah and Pertiwi mother and child hospital, Dr. Wahidin Sudirohusodo,
Pelamonia, Bhayangkara, Syekh Yusuf, Haji and Labuang
Baji hospital from March to September 2014. All subjects fulfilling
the inclusion criteria were taken tissue samples from mothers experiencing
spontaneous abortion and blood samples from normally
born baby. The data were analyzed using Pearson chi-square with
significant rate of 5% (p]]>
<![CDATA[Phosphatase Regenerating Liver3 and ECadherin Expression in Epithelial Ovarian Cancer ]]>
<![CDATA[Maharani, Risma]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Masadah, Rina]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 4, No.3, July 2016 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
Show Abstract
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DOI: 10.32771/inajog.v4i3.442
<![CDATA[Objective: To determine the expression of Phosphatase Regenerating
Liver-3 (PRL-3) and E-Cadherin in the epithelial ovarian cancer
on various stages and differentiation grades.
Method: This was a cross-sectional study design conducted at
Obstetrics and Gynecology Department of several teaching hospitals,
Faculty of Medicine Universitas Hasanuddin from January
to June 2015. The expression of PRL-3 and E-cadherin was
assessed immunohistochemically in 40 patients with epithelial
ovarian cancer including 15 patients in early stage and 25 patients
in advanced stage. We used the Fisher’s exact test with the
significance of p0.05). The significant difference was
found in the expression of E-cadherin whereas the high expression
was shown at early stage than advanced stage (p0.05). This study also pointed out no
correlation between the expression of PRL-3 and E-cadherin in
epithelial ovarian cancer (p>0.05).
Conclusion: PRL-3 overexpression does not decrease E-cadherin
expression in epithelial ovarian cancer.
Keywords: E-cadherin, epithelial ovarian cancer, PRL-3]]>
<![CDATA[Vascular Endothelial Growth FactorC Serum and Endostatin Serum as Predictors of Lympho Vascular Invasion in Early Stage Cervical Cancer ]]>
<![CDATA[Rusmardiani, Andhinna]]>;
<![CDATA[Rauf, Syahrul]]>;
<![CDATA[Lukas, Efendi]]>
<![CDATA[ Indonesian Journal of Obstetrics and Gynecology Volume. 5, No. 2, April 2017 ]]>
Publisher : <![CDATA[Indonesian Socety of Obstetrics and Gynecology ]]>
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DOI: 10.32771/inajog.v5i2.529
<![CDATA[Objective: To determine the relationship of Vascular Endothelial Growth Factors-C (VEGF-C), endostatin and the ratio of VEGF-C/ endostatin with limfo vascular invasion in patient with early stage cervical cancer.
Methods: This study used a cross sectional method. Samples were all patients with early stage cervical cancer who came to the several teaching hospitals of Obstetrics and Gynecology Department Universitas Hasanuddin Medical Faculty that meet the criteria,
then we measured the levels of VEGF-C and endostatin.
Results: We get 30 women with cervical carcinoma. The results showed that the serum levels of VEGF-C was higher in limfo vascular invasion positive (p = 0.017); the ratio of VEGF-C/ endostatin higher in limfo vascular invasion positive (p = 0.004); whereas serum levels of endostatin did not differ significantly in limfo vascular invasion positive or negative (p = 0.522).
Conclusion: The level of VEGF-C and VEGF-C/ endostatin ratio was higher in patients with early stage cervical cancer with positive LVSI than negative LVSI.
[Indones J Obstet Gynecol 2017; 5-2: 105-109]
Keywords: cervix uteri cancer, endostatin, limfo vascular invasion, VEGF-C]]>
