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All Journal Jurnal Fitopatologi Indonesia Cropsaver : Journal of Plant Protection JURNAL AGROTEKNOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Journal of Applied Agricultural Science and Technology JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Jurnal Hilirisasi IPTEKS (JHI) Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks
Zurai Resti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Engineering Environmental Science Health Professions Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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AKTIVITAS ENZIM PEROKSIDASE BAWANG MERAH YANG DIINTRODUKSI DENGAN BAKTERI ENDOFIT DAN TAHAN TERHADAP PENYAKIT HAWAR DAUN BAKTERI (XANTHOMONAS AXONOPODIS PV. ALLII) Resti, Zurai; Habazar, Trimurti; Putra, Deddi Prima; ., Nasrum
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.172 KB) | DOI: 10.23960/j.hptt.216131-137

Abstract

Peroxidase enzyme activity of the introduced shallots with endophytic bacteria and resistant to bacterial leaf blight (Xanthomonas axonopodis pv. allii). Bacterial leaf blight caused by Xanthomonas axonopodis pv. allii is an important disease in shallots. We have earned six isolates of endophytic bacteria, which have the ability to induce systemically resistance to shallots. One mechanism in induce resistance in plants is a change in the plant defense enzyme activity such as peroxidase. The purpose of this study was to calculate the peroxidase enzyme activity of shallots crop is being introduced with endophytic bacteria and is able to induce resistance to Xanthomonas axonopodis pv. allii. This research was conducted by introducing six isolates of endophytic bacteria on shallot bulbs and planted in greenhouse. Shallots crop that was 14 days old then inoculated with the bacterium Xanthomonas axonopodis pv. allii and incubated until symptoms appear. Peroxidase enzyme activity was calculated on the roots and leaves of shallots are 0, 1, 2, 4, 6, 8, 10, 15, and 30 days after inoculation (dai). The results showed an increase in the peroxidase enzyme activity of shallots crop is being introduced by endophytic bacteria compared to the control. Isolates ULG1E2 (Serratia marcescens PPM4) was isolate with the highest peroxidase enzyme activity both in the roots and leaves are 0,051 ìm/ ml.
FORMULASI PADAT RHIZOBAKTERIA INDIGENUS BACILLUS THURINGIENSIS TS2 DAN WAKTU PENYIMPANAN UNTUK MENGENDALIKAN PENYAKIT PUSTUL BAKTERI XANTHOMONAS AXONOPODIS PV. GLYCINES Yanti, Yulmira; Habazar, Trimurti; Resti, Zurai
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 1 (2017): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1732.39 KB) | DOI: 10.23960/j.hptt.1179-18

Abstract

Solid formulations of indigenous rhizobacteria Bacillus thuringiensis TS2 and storage time to control bacterial pustule disease Xanthomonas axonopodis pv. glycines. Bacterial pustule disease caused by Xanthomonas axonopodis pv. Glycines is a major constraint in soybean cultivation. Indigenous rhizobacteria Bacillus thuringiensis TS2 from soybean rhizosphere acquired from previous research is the best isolate which can control soybean bacterial pustule disease and increase growth rate of soybean. To increased its stability and interaction with soybean plants, Bacillus thuringiensis TS2 was urged to test furthermore especially its formulation with based formula tapioca powder, peat and bulk. The most effective storage time also need to test. Result showed that all rhizobacterial formula had ability to decrease incidence of bacterial pustule disease compared to control. Moreover, all the three formula could increase plant growth, total of leaves, total of branch and yields. Flowering time was also advanced by 1-8 days compared to control. Decreasing of disease rate and increasing of plant growth rate variated between different formulations.
CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE Resti, Zurai; Habazar, Trimurti; Putra, Deddi Prima; ., Nasrun
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.334 KB) | DOI: 10.23960/j.hptt.11831-38

Abstract

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.
Penapisan Bakteri Endofit Akar Kedelai Secara in Planta untuk Mengendalikan Penyakit Pustul Bakteri Trimurti Habazar; Zurai Resti; Yulmira Yanti; Jumsu Trisno; Afrika Diana
Jurnal Fitopatologi Indonesia Vol 8 No 4 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.273 KB) | DOI: 10.14692/jfi.8.4.103

Abstract

Endophytic bacteria reside within plant hosts without causing disease symptoms. Bacterial endophytes promote plant growth and yield, suppress pathogens, may help to remove contaminants, solubilize phosphate, or contribute assimilable nitrogen to plants. The aim of this study were to obtain indigenous endophytic bacterial isolates, which have the ability to control bacterial pustule cause by Xanthomonas axonopodis pv. glycines and to increase growth and yield of soybean. The bacterial endophyte isolates were introduced as seed treatment (108 cfu/ml) and soil drench on 3 weeks old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated on one month old of soybean by pricking leaf methodes. The effect of bacterial endophyte on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found two selected bacterial endophyte isolates from soybean’s rhizosphere (St4E1.1 and St1E1.1.), with the ability to control bacterial pustule and to increase growth and yield of soybean.Key words: bacterial endophyte, bacterial pustule, in planta technique, soybean
Formulasi Bakteri Endofit Akar Kedelai untuk Pengendalian Pustul Bakteri Trimurti Habazar; Zurai Resti; Yulmira Yanti; Sutoyo Sutoyo; Imelda Imelda
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.147 KB) | DOI: 10.14692/jfi.11.2.51

Abstract

Two isolates of bacterial endophyte from soybean root were found to be effective to control bacterial pustule caused by Xanthomonas axonopodis pv. glycines. Formulation of the bacterial isolates is required to maintain the effectivity of this bacterial isolates during storage, transportation and application. The aim of this research was to obtain the best carrier for formulation to maintain the effectivity of bacterial endophyte in storage to control bacterial pustule on soybean. Three kind of carrier agent was evaluated for formulation of bacterial endophyte i.e. peat soil, tapioca flour and coconut water + 1% palm oil. Each carrier agent was combined with 5 treatment of storage time i.e. 0, 1, 3, 5 and 7 weeks. Soybean plants was inoculated by. X. axonopodis pv. glycines 2 weeks after planting by rubbing bacterial suspension (106 cel mL-1) on lower surface of leaves. The results showed that all formulas of bacterial endophyte were able to suppress the bacterial pustule on soybean. The best formulations were bacterial endophyte in peat soil stored for 1 and 7 weeks (effectivity rate 79.85% and 77.02%) and coconut water + palm oil and storaged for 3 weeks (effectivity rate 77.46%).
PENGARUH INTRODUKSI ISOLAT RIZOBAKTERIA INDIGENUS TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KENTANG (Solanum tuberosum L.) DAN DINAMIKA POPULASI GULMA DI ALAHAN PANJANG, SUMATERA BARAT FRISKIA HANATUL QOLBY; IRAWATI CHANIAGO; INDRA DWIPA; ZURAI RESTI
Jurnal Agroteknologi Vol 11, No 1 (2020): Agustus 2020
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v11i1.9411

Abstract

Potato (Solanum tuberosum L.) is of important major crops for its carbohydrate and plays important roles to support food sovereignty along with rice, wheat, corn, and sorghum. Potato growth and yield at West Sumatra has not reached its maximum potential due to some factors including cultivation. One major important constraint in potato growing in West Sumatra is the fact that farmers use the 8th to 12th generation of potato seedlings which have a very low agronomic potential. Yet, weeds have caused another problem in the field and reduce potato yield. An experiment has been conducted at the Laboratory of Microbiology, Faculty of Agriculture, Universitas Andalas and Nagari Alahan Panjang, Subdistrict Lembah Gumanti, Solok Regency from November 2018 to March 2019. The experiment was aimed at determining the best indigenous rhizobactria isolates to increase the growth and yield of potato plants as well as weed population dynamics associated with potato plants. Six isolates of indigenous rhizobacteria and a control treatment group were tested in a completely randomised design with three replicates. The isolates were L1 S3.1, L1 S3.2, L1 S4.4, L2 S1.2, L2 S2.3, L2 S3. 2. Data were analyzed with analysis of variance and mean separation with Duncan's New Multiple Range Test (DNMRT) at 5%. Results showed that isolate L1 S4.4 was best in increasing the number of branches, number of tubers, and weight of potato yield. The isolates tested affected weed populations dynamics, prior to treatments there were 8 species of weeds found at the field i.e Bidens pilosa L., Leptochloa chinensis, Eleusine indica L., Agerathum conyzoides, Richardia sp, Galinsoga parviflora, and Sonchus arvensis. During potato growing at the field, weed species of Eleusine indica L. and Gynura divaricata dissappeared from the soil. In contrast,  Amaranthus spinosus emerged demonstrating that weed population dynamic has occurred.
SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH Zurai Resti; Trimurti Habazar; Deddi Prima Putra; . Nasrun
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.607 KB) | DOI: 10.23960/j.hptt.213167-178

Abstract

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot. The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.
Penapisan Isolat Rizobakteri dari Perakaran Tanaman Kedelai yang Sehat untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis pv. glycines) Yulmira Yanti; Trimurti Habazar; Zurai Resti; Dewi Suhalita
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.092 KB) | DOI: 10.23960/j.hptt.11324-34

Abstract

Screening of indigenous rhizobacteria from healthy soybean root to control bacterial pustule (Xanthomonas axonopodis pv. glycines) using in planta technique. Plant growth promoting rhizobacteria are a group of bacteria that actively colonize plant roots, increase plant growth and control plant pathogens. The aim of this study was to obtain rhizobacteri isolates which have the ability to control bacterial pustule and increase growth and yield of soybean. This method based on in planta selection of enhanced competitive soil root-colonizing bacteria from soil samples of healthy soybean root at endemic area of bacterial pustule in Darmasraya District and Sijunjung District, West Sumatera. We characterized only the best rhizobacteri isolates which have ability to control bacterial pustule and to increase growth and yield of soybean. This type of characterization has possibility to find new, easy and cheap biocontrol organisms. Ten Rhizobacteri isolates were introduced via seed treatment (108 cfu/ml) and soil drench to 3 week old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated to one month old of soybean seedling. The effect of rhizobacteria on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found that two selected rhizobacteri isolates from soybean (P12Rz2.1 and P14Rz1.1) were the best isolates in promoting growth and the of the soybean plants with the effectiveness 20.62 % and 20.47 %.
APLIKASI INSEKTISIDA BERBAHAN AKTIF BUPROFEZIN TERHADAP WERENG BATANG COKLAT (WBC) DI KELTAN RAMBUTAN DAN KELTAN SAKATO KOTA PADANG Nurfitri Sari; My Syahrawati; Arneti Arneti; Zurai Resti; Martinius Martinius; Haliatur Rahma; Eri Sulyanti; Elfitri Syahdia
Jurnal Hilirisasi IPTEKS Vol 2 No 3.b (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.706 KB) | DOI: 10.25077/jhi.v2i3.b.326

Abstract

Wereng batang coklat atau WBC (Nilaparvata lugens Stal.) merupakan salah satu hama utama pada tanaman padi yang dapat menyebabkan kerusakan dalam waktu relatif singkat. Serangan hama ini mulai mengemuka di Sumatera Barat sejak tahun 2009-2017. Insektisida berbahan aktif buprofezin adalah salah satu insektisida yang banyak digunakan untuk mengendalikan populasi WBC. Pelatihan cara menakar dan aplikasi buprofezin ini telah dilaksanakan di Keltan Rambutan Kelurahan Bungus Barat dan Keltan Sakato Kelurahan Lambung Bukit, Kota Padang. Tujuannya adalah untuk memperlihatkan kepada petani kemampuan insektisida berbahan aktif buprofezin selama 1 jam terhadap 10 ekor WBC yang disediakan. Perlakuannya berupa aplikasi buprofezin pada dosis tertentu (½ dosis anjuran, dosis anjuran, 1½ dosis anjuran), masing-masing dalam 5 ulangan. WBC yang digunakan merupakan populasi lapangan dari lahan persawahan Kecamatan Pauh. Hasil pengujian di Keltan Rambutan menunjukkan bahwa insektisida sesuai dosis anjuran mematikan 20% WBC, dan di Keltan Sakato mematikan 24% WBC, dosis anjuran mematikan 26% WBC dalam satu jam. Dosis anjuran Buprofezin memang tidak ditujukan untuk mematikan 100 % wereng uji, namun lebih kepada dampaknya terhadap penghambatan ganti kulit 2-3 hari kemudian.
Endophytic Bacterial Consortia as Biological Control of Bacterial Leaf Blight and Plant Growth Promoter of Rice (Oryza sativa L.) Zurai Resti; Yenny Liswarni; Martinius Martinius
Jurnal Ilmu dan Teknologi Terapan Pertanian Vol 4 No 2 (2020): August 2020
Publisher : Politeknik Pertanian Negeri Payakumbuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32530/jaast.v4i2.146

Abstract

The consortia of endophytic bacteria with various mechanisms (competition, antibiotics, induction of resistance, and others), simultaneously, are more effective in controlling pathogens and increasing plant growth. The purpose of this study was to obtain endophytic bacterial consortia capable of suppressing of bacterial leaf blight and promoting the growth of rice plants. The study consisted of three experimental stages. The first stage was the test on the antibiosis ability of the endophytic bacterial consortia to suppress of pathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) using the Kirby Bauer method. The second stage was the test on the ability of endophytic bacterial consortia to promote the growth of rice seedlings. The experiment was arranged in a Completely Randomized Design (CRD) which consisted of six combinations of endophytic bacterial consortia and 15 replications within each combination. The endophytic bacterial consortia were introduced by soaking the seeds, and the observations were made on the plant height, number of leaves, root length, fresh weight, and dry weight of rice seedlings. Meanwhile, the third stage was the test on the ability of endophytic bacterial consortia to suppress of bacterial leaf blight (BLB) diseases and to promote the rice plant growth. The experiment was arranged in a Completely Randomized Design (CRD) with six combinations of endophytic bacterial consortia and five replications within each combination. The endophytic bacterial consortia were introduced by soaking the roots of the seedlings. The observations were made on the incidence of disease, disease severity, number of leaves, plant height, and number of tillers. The results showed that all endophytic bacterial consortia had antibiosis abilities. The best endophytic bacterial consortia for controlling bacterial leaf blight and promoting the growth of rice seedlings and plants were the C (Bacillus sp SJI; Bacillus sp HI) and D (Bacillus sp SJI; S.marcescens isolate JB1E3) consortia.
Co-Authors . Nasrun Afdil Rahman Afrika Diana Arneti Arneti Aziz Fauzi Deddi Prima Putra Dewi Suhalita Elfitri Syahdia Eri Sulyanti FRISKIA HANATUL QOLBY Haliatur Rahma, Haliatur Hasmiandi Hamid Indra Dwipa Irawati Chaniago Jumsu Trisno Martinius Martinius Martinius Martinius Martinius Martinius Murdiman, Imelda MY SYAHRAWATI Nasrum ., Nasrum Nasrun . Nurfitri Sari Ryan Hidayat Sandra Desiska Sutoyo Sutoyo Tre Julia Nasral TRIMURTI HABAZAR Yenny Liswarni Yenny Liswarni Yulmira Yanti