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All Journal Jurnal Natur Indonesia BIOMA : Jurnal Biologi Makassar Jurnal EBONI
Muh Restu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science

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Optimalisasi Teknik Ekstraksi dan Isolasi DNA Tanaman Suren (Toona Sureni Merr.) untuk Analisis Keragaman Genetik berdasarkan Random Amplified Polymorphic DNA (RAPD) Restu, Muh; Mukrimin, Mukrimin; Gusmiaty, Gusmiaty
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.65 KB) | DOI: 10.31258/jnat.14.1.138-142

Abstract

The species of trees have different secondary compounds that need optimum extraction techniques. Appropriate extraction techniquesdetermine the quality and quantity of DNA produced. This research aims to found optimal of extraction methods and DNA isolation, thento created genome DNA in high quality and quantity, so that it can be using for genetic variation analyses in Suren (Toona sureni Merr.) byRandom Amplified Polymorphic DNA (RAPD). This study shows that DNA concentrates were 763.3 μg/ml, 180.0 μg/ml, 383.3 μg/ml, and436.7 μg/ml. While based on the results of PCR amplification using the primers OPD 03 shows that the four extraction methods used, the extraction method of number 3 has been able to produce genomic DNA with better quality and more number of bands, although the quantityis lower.
Eksplorasi Cendawan Rhizosfer Pada Tegakan Hutan Rakyat suren Untuk Meningkatkan Pertumbuhan Tanaman Payangan Yanti Rina; Gusmiaty Gusmiaty; Muh Restu
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 4 No. 2 (2019)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v4i2.8397

Abstract

Pertumbuhan tanaman dapat dipengaruhi oleh adanya keragaman mikroba yang yang terdapat di rhizosfer, salah satunya yaitu cendawan. Cendawan rhizosfer yang terdapat pada akar tanaman dapat meningkatkan pertumbuhan tanaman dan perlindungan terhadap mikroba tertentu. Penelitian ini bertujuan untuk mengidentifikasi cendawan rhizosfer. Metode penelitian yang digunakan yaitu isolasi cendawan menggunakan teknik pengenceran 10-2 dan 10-3 , serta identifikasi cendawan berdasarkan karakter morfologi. Hasil isolasi cendawan rhizosfer diperoleh sebanyak 33 isolat yang terdiri atas 5 genus yaitu Aspergillus, Trichoderma, Rhizopus, Penicillium dan Fusarium.Kata Kunci : Suren, Rhizosfer, Cendawan
APLIKASI BERBAGAI ZAT ANTIOKSIDAN SEBAGAI PENGHAMBAT BROWNING MEDIA TANAM EKSPLAN JATI PUTIH (Gmelina arborea Roxb) SECARA IN-VITRO Gusmiaty Guntur; Muh Restu; Mutiah Ummusyahidah AR
Jurnal Eboni Vol 1 No 1 (2019): November
Publisher : Program Studi Kehutanan Universitas Muslim Maros

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.2 KB)

Abstract

Browning is the most problem in plant tissue culture, both in growing media and explants. Browning is formed by oxidation process of phenolic compounds thatcan caused tissue death. The objective of this study were to analyze the effect of antioxidants addition against browning and to determine the best antioxidant toinhibited browning of Gmelina arborea explant growing media. The research was conducted by two steps, namely preparation of Murashige and Skoog (MS) media which are added 2,4-D growth regulators and addition of antioxidant PVP, ascorbic acid and activated charcoal. The results indicated that the addition ofantioxidants in the media were able to inhibit browning process of the media as well as in the explant. Moreover, the ascorbic acid performed better ability to inhibit browning process compared to the other antioxidants.
Co-Authors Gusmiaty Guntur Gusmiaty Gusmiaty Gusmiaty Gusmiaty, Gusmiaty MUKRIMIN MUKRIMIN Payangan Yanti Rina Ummu Syahidah