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Histology of Liver and Kidney of Mystus nemurus that immersed with Curcuma xanthorrhiza, ROXB extract Lubis, Fauzi Ahmad; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 1, No 2 (2014): Wisuda Oktober Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was carried out in November 2013 until January 2014 in the laboratory of Fish Diseases and Parasites, Faculty of Fisheries and Marine Science Riau University Pekanbaru. The purpose of this research was to understand the histological structure of liver and kidney of Mystus nemurus that is immersed with Curcuma xanthorrhiza ROXB extract. The method used in this study was experimental and the histologycal structure of the fish will be analyzed descriptively. The treated fish were immerse in 3 different concentration of Curcuma xanthorrhiza ROXB extract, they were (P1 (0.2 g/L), P2 (0.4 g/L, P3 (0.6 g/L). for five minutes/ day for 30 days period. The negative control were fishes that were not treated with Curcuma xanthorrhiza ROXB extract. The liver showed hemorhage, fatty degenerative and necrosis. The kidney showed necrosis and hemorhage. It was concluded that immersion of fish in Curcuma xanthorrhiza ROXB extract shown histologycal alterations in the tissue of Mystus nemurus.Key words: Mystus nemurus, Curcuma xanthorrhiza ROXB, kidney, liver

Histopatologi Ginjal Ikan Jambal Siam (Pangasiodon hypophthalmus) yang Diberi Pakan Mengandung Tepung Kunyit Wahyuni, Sri; Riauwaty, Morina; Windarti, Windarti
Jurnal Perikanan dan Kelautan Vol 25, No 3 (2020)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.25.3.232-237

Turmeric is an herbal plant that has antibacterial and antioxidant abilities, and also to improve the fish's immune system. The aim of this research was to look at the histological structure of kidney and survival rate fish which was given feed with turmeric flour before and after infected with A. hydrophila. This research was conducted from February to May 2019 in the Laboratory of Parasites and Fish Diseases of the Faculty of Fisheries and Marine Sciences, University of Riau. The method in this research was the experimental method by one-factor Completely Randomized Design (CRD) with five levels. Kn (feeding without given turmeric flour and without infection with A. hydrophila), Kp (Feeding without given turmeric flour and infected with A. hydrophila), P1 (turmeric flour at a dose of 0.5 g / kg of feed), P2 (dose of 0.7 g / kg), P3 (dose of 0.9 g / kg). The results showed that the histological structure of catfish which was given the addition of turmeric flour in feed by normal conditions and after being challenged with A. hydrophila showed some damage to the gill and kidney structure. The best dose of adding turmeric flour to feed is 0.7 g / kg of feed (P2), seen from the structure of the kidney cell, and the best survival. Histological structure of kidney cell structure in P2 treatment has abnormality such as hemorrhage and survival rate 100%.

LEUKOCYTES DIFFERENTIATION OF Pangasius hypopthalmus THAT WERE FEED WITH CURCUMIN EXTRACT FROM Curcuma domestica V Iman, Khasan Nur; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted on June until August 2016 in Parasites and Fish Diseases Laboratory, Faculty of Fisheries and Marine science, University of Riau, Pekanbaru. The aim of this research was to determine leucocyte differentiation of Pangasius hypopthalmus that were feed with curcumin extract from Curcuma domestica V. This research used five treatment such as; Kn (negative control): that were feed without curcumin extract and were not infected with A. hydrophila, Kp (positive control): that were feed without curcumin extract and infected with A. hydrophila, P1: feed with curcumin extract in 0,3g/kg, P2: feed with curcumin extract in 0,5g/kg and P3: feed with curcumin extract in 0,7 g/kg. Fish reared in an aquarium the size of 40x30x30 cm with stocking density 10 fish / aquarium, feeding as many as 10% of body weight and do 3 times a day. Pisciculture carried out for 30 days and after that fish infected with Aeromonas hydrophila. The results shown that P3 (feed with curcumin extract in 0,7 g/kg) were the best treatment with lymphocytes 83,00%; monocytes 5.33%; neutrophils 3,67%; thrombocyte 8.00%; total leukocytes 12,01x104 cell/mm3; and fagocyte index 58,33.Keyword: Leukocytes, Pangasius hypopthalmus, Curcuma domestica V, Curcumin Extract

EFFECTIVENESS OF TRIPLOIDIZATION ON INGIR-INGIR (Mystus nigriceps) WITH DIFFERENT FERTILIZATION AND HEAT SHOCK Haloho, Mei Dinar Tauriska; Riauwaty, Morina; ', Nuraini
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted in August until November 2015 in the Fish Hatchery and Breeding Laboratory, Faculty of Fisheries and Marine Sciences, University of Riau, Pekanbaru. This research was aimed to know the triploidization effectiveness in fertilized egg of ingir-ingir larvae (Mystus nemurus) with different fertilization and heat shock. The method used in this research was the experimental method; 2 factors, 10 treatments and 3 replications. Fresh eggs and milt were fertilization, then 1, 2 and 3 minutes after fertilization zygotes were shocked by heat shock 400C for 1, 2 and 3 minutes. The treatments were, P0K0 (as control), P1K1, P1K2, P1K3, P2K1, P2K2, P2K3, P3K1, P3K2, P3K3. The result shown that the best results in treatment Fertilization Rate (P3K1=31,06%), Hatching Rate (P2K1=65,74%), Survival Rate of 4 days and Survival Rate of 21 days (P3K3=71,82% and 41,67%), the length and width of a red blood cell were (0.192 mm and 0.123 mm).Key Words: Triploidization, Mystus nemurus, Old Fertilization, Old Surprise, Heat Shock

THE SENSITIVITY OF MANGROVE Rhizophora sp. STEM SKIN EXTRACT ON Aeromonas salmonicida Jampil, Tirta Herlis; Syawal, Henni; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The research was conducted in July 2016 to Januari 2017 in the Laboratory of Parasites and Fish Disease of Fisheries and marine science University of Riau, Integrated Chemical Laboratory the Faculty of Mathematics and Natural Science of Health Muhammadyah University of Riau and Fish Quarantine Station, Quality Control and Safety of Fishery Products clas I Pekanbaru. The purpose of this study was to sensitivity determine the ability of Rhizophora sp. stem skin extracted can inhibit the growth of bacteria A. salmonicida and to know the toxicity of Rhizophora sp. stem skin extract against gold fish, the LD50 test was conducted by immersion for 24 hours. Sensitivity test is using the method of Kirby bauer disk blank 6 mm disc with dose used were 10000 ppm, 9000 ppm, 8000 ppm, 7000 ppm, 6000 ppm, 5000 ppm, 4000 ppm, 3000 ppm, 2000 ppm, 1000 ppm and control Ampicilin. The result shown that the Rhizophora sp. stem skin extract can inhibit the growth of bacteria A. salmonicida that in the dose of 1000 ppm with 7,90 mm clear zone . MIC test showed this resueld is 800 ppm has a number of colonies of bacteria aqual 219.66 x 108 CFU/mL. Is able to inhibit the growth of bacteria A. salmonicida. Dose LD50 stem skin extract Rhizophora sp. against gold fish by immersion for 24 hours in 750 ppm.Keyword: Rhizophora sp., Sensitivity, Minimum Inhibitory Concentration, Aeromonas salmonicida

EFFECTS OF FEEDING FEED ENRICHED CURCUMIN TURMERIC (Curcuma domestica Val) OVERVIEW OF THE RED BLOOD CELLSPangasius hypophthalmus Malau, Diana P; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted in June-August 2016. The purpose of this study was todetermine the effect of feeding feed enriched curcumin turmeric (Curcuma domestica Val)to the picture of the red blood cells (total erythrocytes, hematocrit, and hemoglobinconcentration) Pangasiushypophthalmus and infected by Aeromonas hydrophilla. Thedesign used was Completely Randomized Design (CRD) with one factor, the level 5treatments and 3 replications. The treatments were feeding enriched curcuminextract withdifferent dose i.e Kn = negative control (feeding without being given curcumin extract andwere not infected byAeromonashydrophilla); Kp = positive control (feeding without beinggiven curcumin extract and infected byAeromonashydrophilla); P1 = addition of curcuminextract in feed at a dose of 0.3 g/kg; P2 = addition of curcumin extract in feed at a dose of0.5 g/kg; and P3 = addition of curcumin extract in feed at a dose of 0.7 g/kg. The resultsshowed that feeding enriched curcuminextract against of Pangasiushypophthalmus at adose of 0.7 g/kg (P3) is the best dose for Pangasiushypophthalmuswith a total oferythrocyte 1.87x106 cells/mm3, hematocrit 35.33 %, and hemoglobin concentration of 7.66g/dl after infected byAeromonashydrophilla and generate growth in absolute weight of 3.66g and a survival rate of 100 %.Keywords:Curcuminextract, Pangasius hypophthalmus, Aeromonas hydrophila, Totalerythrocytes,

HISTOPATHOLOGY LIVER AND KIDNEY OF Clarias gariepinus THAT ARE FED SIMPLISIA MANGOSTEEN RIND (Garcinia mangostana L) Kahfi, Khaiqal El; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This study has been conducted on Maret to June 2016 in at Parasite and Fish DiseaseLaboratory, Faculty of Fisheries and Marine Sciences, University of Riau. Theaims of this study was to know changes in liver and kidney tissue structures of catfish(Clarias gariepinus) fed simplisia mangosteen rind. This study used an experimentalmethod and histological of the liver and kidney structure of Clarias gariepinus wereanalyzed by descriptive. The treated fishes were fed in three differentconcentrations of fed simplisia mangosteen rind. Kn: (feed control) the fish were notfed simplisia mangosteen rind, P1: The fish were fed simplisia mangosteen rind 2g/kg, P2: The fish were fed simplisia mangosteen rind 4 g/kg, P3: The fish were fedsimplisia mangosteen rind 6 g/kg and fishes were reared for 60 days. Showed that theabnormalities of liver and kidney such us inflammatory cells, haemorrhage,h yp e r t r h o p y and necrosis. Base on data obtain it can be concluded that the bestconcentration of simplisia mangosteen rind 2 g/kg is able to prevent damage of liverand kidney after given fed simplisia mangosteen rind.Key words : Clarias gariepinus, simplisia mangosteen rind, histopathology,kidney, liver

HISTOPATHOLOGY LIVER AND KIDNEY OF Clarias gariepinus THAT ARE FED SIMPLISIA MANGOSTEEN RIND (Garcinia mangostana L) Kahfi, Khaiqal El; Riauwaty, Morina; Lukistyowati, Iesje
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This study has been conducted on Maret to June 2016 in at Parasite and Fish DiseaseLaboratory, Faculty of Fisheries and Marine Sciences, University of Riau. Theaims of this study was to know changes in liver and kidney tissue structures of catfish(Clarias gariepinus) fed simplisia mangosteen rind. This study used an experimentalmethod and histological of the liver and kidney structure of Clarias gariepinus wereanalyzed by descriptive. The treated fishes were fed in three differentconcentrations of fed simplisia mangosteen rind. Kn: (feed control) the fish were notfed simplisia mangosteen rind, P1: The fish were fed simplisia mangosteen rind 2g/kg, P2: The fish were fed simplisia mangosteen rind 4 g/kg, P3: The fish were fedsimplisia mangosteen rind 6 g/kg and fishes were reared for 60 days. Showed that theabnormalities of liver and kidney such us inflammatory cells, haemorrhage,h yp e r t r h o p y and necrosis. Base on data obtain it can be concluded that the bestconcentration of simplisia mangosteen rind 2 g/kg is able to prevent damage of liverand kidney after given fed simplisia mangosteen rind.Key words : Clarias gariepinus, simplisia mangosteen rind, histopathology,kidney, liver

LEUKOCYTES DIFFERENTIATION OF Pangasius hypophthalmus THAT WERE IMMERSED IN Lawsonia inermis L. Hasugian, Sampang Hotramadi; Riauwaty, Morina; Syawal, Henni
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 1 Januari s/d Juni 2018
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT The research was conducted in June to October 2017 in Parasites and Fish Diseases Laboratory Faculty of Fisheries and Marine Science, University of Riau. The purpose of this research was to determine total leucocyt, leukocytes differentiation, and phagocytosis index of Pangasius hypophthalmus. There were five treatments applied namely negative control (Kn) was the fishes were not infected with Aeromonas hydrophila and were not immersed with Lawsonia inermis L. solution, positive control (Kp) was the fishes were not immersed with Lawsonia inermis L. solution but were infected with Aeromonas hydrophila. The dose of Lawsonia inermis L. solution were 2000 ppm (P1), 3000 ppm (P2), 4000 ppm (P3). The fishes were immersed for five minute in once a week for five times. Based on the data, it can be uncluded that the immersion with Lawsonia inermis is able to increace the immune system of the Pangasius hypophthalmus. The best dose was at 2000 ppm wich was shown by lymphocyte 83,33%, neutrophils 8,33%, monocyte 8.33%, total leukocyte 11,27x104 cell/mm3, fagocyte index 63,33%, the average of weight 6,97 g and survival rate 93,33%. Keyword: Leukocytes, Pangasius hypophthalmus, Lawsonia inermis L, phagocytosis, Aeromonas hydrophila

SENSITIVITY OF THE JUICE OF KAFFIR LIME FRUIT (Citrus hystrix) AGAINST BACTERIA Aeromonas hydrophila Sihombing, Jefry Maju Arytha; Lukistyowati, Iesje; Riauwaty, Morina
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACTÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Disease that often develops in aquaculture is one of them is red spot disease.This disease is often calledMotile Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila. Alternative ingredients that can be used as prevention or treatment of fish diseases arekaffir lime plant (Citrus hystrix) which is antibacterial and antimicrobial.This research was conducted in February - June 2018 in the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, University of Riau. The purpose of this study was to determine the sensitivity of the juice of kaffir lime to inhibit the growth ofAeromonas hydrophila bacteria, dosage of Minimum Inhibitory Concentration (MIC), and continued the LD50test obtained from the MIC dose where the test of LD50solution of kaffir lime on catfish (Pangasius sp.) by immersion. The research method used was the experimental method, for the sensitivity test using the Kirby Bauer disc method with the concentrations used were 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% , 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.9%, 0.8%, 0.7%, 0.6% and controls ( Oxytetracycline). The results showed that the juicce of kaffir lime was able to inhibit the growth of A. hydrophila bacteria starting from doses of 100% - 0.6% with an average diameter of inhibition zones ranging from 23.03 - 6.68 mm. The dose of Minimum Inhibitory Concentration (MIC) juice of kaffir lime fruit is 0.7% with an average number of colonies of 289.33 CFU / mL. The test results of LD50 juice of kaffir lime (C. hystrix) on catfish (Pangasius sp.) By immersion method for 24 hours were at a concentration of 0.791%.Â Keywords:C.hystrix, Motil Aeromonas Septicemia, Kirby Bauer discs, Minimum Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Inhibitory Concentration (MIC), LD50

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