Afriani Afriani
Universitas Andalas

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Correlation between N-Acetyltransferase 2 (NAT2) Polymorphism Genotype with Plasma Isoniazid (INH) Concentration in MDR TB Patients Receiving Short Regimen in West Sumatera Mega Senja; Masrul Basyar; Yessy Susanty Sabri; Afriani Afriani
Jurnal Respirologi Indonesia Vol 42, No 1 (2022)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v42i1.283

Abstract

Background: Isoniazid (INH) is one of the most potent TB drug. High dose INH is used in short regimen MDR TB drugs. The genetic polymorphism of NAT2 affects the acetylation status. Awareness of the patients’ acetylator status is important to determine the risk of toxicity, treatment failure and drug resistance. The aim of this study was to demonstrate NAT2 genotype association with INH plasma concentration after 2 hours of oral INH therapy. Methods: This was a cross sectional study of MDR TB patients who received short term combination therapy at RSUP Dr. M.Djamil Padang, Achmad Muchtar Hospital Bukittinggiand West Sumatra Pulmonary Hospital from September 2019 to February 2020. Patients were examined for NAT2 genotype and plasma INH concentration. The results of the plasma INH concentrations obtained were evaluated based on the NAT2 acetylator phenotype group.Results: The majority of the subjects weremen (62.5%), aged 40-64 years (50%), had the most common comorbid of diabetes mellitus (31.25%), were normoweight (75%) and had negative HIV status (93.8%). A total of 7 alleles consisting of 7 SNPs and 7 variations of the NAT2 genotype were found in MDR TB patients who received short-term therapy. The NAT2*12A alleles (56.25%) was the most common allele and was a fast acetylator. Based on the bimodal distribution, the median concentration of INH in the fast and slow acetylator were 1.25 µg/ml and 5.24 µg/ml, respectively. The median values of INH concentration based on the trimodal distribution for fast, intermediate, and slow acetylators were 1.25 µg/ml, 2.17 µg/ml and 5.24 µg/ml. Conclusion: There were no correlations between the type of NAT2 acetylator phenotype and plasma INH concentrations.
Accuration of Circulating Tumor DNA (ctDNA) in Detection of Epidermal Growth Factor Receptor (EGFR) Mutation in Lung Cancer Patient Type Adenocarcinoma in M Djamil Hospital Padang Yandra Darusman; Sabrina Ermayanti; Sari Nikmawati; Afriani Afriani
Respiratory Science Vol. 1 No. 1 (2020): Respiratory Science
Publisher : Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/respirsci.v1i1.6

Abstract

Background: ctDNA is an alternative test for detecting mutation of EGFR in lung cancer type adenocarcinoma if the tissue speciment can not be carried out. Sensitivity, specificity and accuration of ctDNA test is stil varied. This study is aimed to acknowledge sensitivity, specificity and accuration of ctDNA in detecting EGFR mutation in patient with lung cancer type adenocarcinoma in M Djamil Hospital. Methods: Design this study a diagnostik test comparing ctDNA to tissue speciment in detection EGFR mutation of 42 patients with lung cancer type adenocarcinoma in M Djamil Hospital. Sample was selected through consecutive technique. Results: Incidence of EGFR mutation in patients with lung cancer type adenocarcinoma from tissue speciment was higher than ctDNA ((42,9% vs 28,6%; p=0,031). There was significant diffierence of EGFR mutation detection between sex, smoking status, and TNM staging based on tissue/cytology examination and ctDNA (p=0,031). EGFR mutation in sitologic test and ctDNA was more likely detected in male patient (66.7% and 58.3%), ex-smoker (50% and 41.7%) and stage IV (88.9% and 91.7%). The results of sensitivity, specificity positive prediction value (PPV) and Negative prediction value (NPV) in ctDNA test to detect EGFR mutation were 66,7%, 100%, 100% dan 80% according to sitology test as gold standard. Furthermore, the ctDNA accuration was measured according to AUC score 0.833 (SE 0,072, CI 95%, 0,693-0,974, p=0.0001). Conclusion: ctDNA test have a good accuration with sensitiviy 66.7% and specificity 100% in detecting EGFR mutation in patients with lung cancer type adenocarcinoma.