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Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria Nia Kurniawaty; Purnama Hidayat; Aunu Rauf
Jurnal Entomologi Indonesia Vol 14 No 1 (2017): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.249 KB) | DOI: 10.5994/jei.14.1.20

Abstract

Thrips is the second largest genus on the order of Thysanoptera, most of them are polyphagous and some species are serious pests on vegetables. The damages caused by thrips can reach 30–50% yield loss. Thrips alliorum (Priesner), T. hawaiiensis (Morgan), and T. parvispinus (Karny) are widely reported as pests on crops, especially on horticulture. This study aimed to identify three trhips species: Thrips alliorum, T. hawaiiensis, and T. parvispinus based on the DNA sequences of mtCOI gene fragment. Thrips samples were collected from Bandung, Bogor, Cianjur, and Kuningan districts. Three steps, they were sample collection and DNA total extraction, amplification by using PCR and DNA sequence analysis. The PCR succesfully amplified DNA of mtCOI gene fragments of T. alliorum, T. hawaiiensis, and T. parvispinus at 678, 690, and 668 bp respectively. The mtCOI DNA sequences were dominated by A and T bases with the nucleotide variation value of  25.18%. Identification of the three thrips species: T. alliorum, T. hawaiiensis, and T. parvispinus based on molecular characters using mtCOI DNA sequences confirmed the identification result based on the morphological caharacters.
Kutudaun (Hemiptera: Aphididae) pada gulma di sekitar lahan pertanian di Jawa Barat beserta kunci identifikasinya Yani Maharani; Purnama Hidayat; Aunu Rauf; Nina Maryana
Jurnal Entomologi Indonesia Vol 15 No 2 (2018): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.2.68

Abstract

Aphids (Hemiptera: Aphididae) are considered as one the most important pest in the world. Some species of aphids were reported as serious pests and plant virus vectors, especially on horticultural crops. Weeds in the agricultural area can serve as an alternative hosts for some aphids which are plant pests or plant virus vectors. The objective of this research was to identify aphid species of weeds in the agricultural production area in West Java and to provide the identification key. Aphids collection was done on the weeds in the agricultural fields in 9 districts of West Java. Aphids were collected from 13 species (5 families) of weeds. The identification of aphid was peformed based on the adult specimens. The research manased to come up with 12 species of aphid on weeds. Three of the 12 collected species were Epameibaphis frigidae (Oestlund), Metopolophium dirhodum (Walker), and Pseudaphis sijui (Eastop) has never been reported in West Java. Six species were known as vectors of plant viruses in agricultural crops. The most commonly found aphid species was Schizaphis graminum (Rondani) in the yard grass (Eleusine indica).
Biologi, neraca hayati, dan pemangsaan Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) pada Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae) Eka Wahyuningsih; Aunu Rauf; Sugeng Santoso
Jurnal Entomologi Indonesia Vol 16 No 1 (2019): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (568.989 KB) | DOI: 10.5994/jei.16.1.18

Abstract

Papaya mealybug, Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae), is an invasive pest that was detected for the first time in Indonesia in 2008. One of the common natural enemies is the predatory beetle Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae). The research was conducted with the objectives to determine the biology, life table parameters, and predation of C. montrouzieri on P. marginatus. Observation of biological and life table parameters and predation was conducted by introducing the predators into Petri dishes containing mealybugs. Eggs of C. montrouzieri hatched in 4.35 days. Mean developmental periods for 1st, 2nd, 3rd, and 4th instar larvae were 4.23; 4.21; 5.84; 5.93 days, respectively. The mean pre-pupal and pupal periods were 1.95 and 7.55 days, respectively. The longevity of male adults was 51.34 days, while that of females was 83.39 days. The number of eggs laid by a single female averaged 198.49. The net reproductive rate (Ro) was 117.11 individu/female/generation, intrinsic rate of increase (rm) was 0.073 individu/female/day, mean generation time (T) was 64.776 days, doubling time (Dt) was 9.426 days, and finite rate of increase (λ) was 1.076 per day.  The 4th instar larvae of C. montrouzieri was the most voracious with numbers of P. marginatus eggs, 1st, 2nd, 3rd instar nymphs or adults consumed were 188.0; 53.4; 44.0; 26.8; or 15.6; respectively. Manly’s preference index suggested that larvae, as well as adults of C. montrouzieri, preferred eggs and 1st instar nymphs of P. marginatus over other mealybug stages. Our study indicates the high potential of C. montrouzieri in the biological control of P. marginatus.
Serangan Arthroschista hilaralis Walk. (Lepidoptera: Pyralidae) pada tegakan jabon (Neolamarckia cadamba [Roxb.] Bosser) di Sumatera Selatan Sri Utami; Hermanu Triwidodo; Pudjianto Pudjianto; Aunu Rauf; Noor Farikhah Haneda
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (744.218 KB) | DOI: 10.5994/jei.15.1.1

Abstract

Infestation of pest is a major problem in the seedling and field of Jabon (Neolamarckia cadamba [Roxb.] Bosser) in Indonesia, especially in South Sumatra. Arthroschista hilaralis Walk. (Lepidoptera: Pyralidae) is one defoliator pest attacked jabon stands. Some information about the infestation of A. hilaralis are required for pest control and prevent. The objective was to study the incidence level and intensity level of A. hilaralis on jabon stands at the planting age 2 and 4 years at the 2 seasons, and analyze the effect of weather (temperature and humidity) to foliar damage caused by A. hilaralis. This experiment was conducted in six locations in South Sumatra. The plots were delineated in three area that each plot was 0.03 ha in area and contain 20 jabon trees. Observations on the incidence and intensity level of A. hilaralis were conducted every month for 16 months representing two categories of time point observation of the rainy season and dry season. The results showed that infestation of A. hilaralis on jabon stands at the planting age 2 years and the rainy season was higher than planting age 4 years and another season. The incidence level and intensity level of A. hilaralis were 74.45% and 55.21% respectively. Weather factors (e.g. temperature and humidity) thought affected the incidence level and intensity level of A. hilaralis. Temperature has negative correlation to the incidence level of A. hilaralis (r = -0.629; p = 0.009) and to the intensity level of A. hilaralis (r = -0.546; p = 0.029). Humidity has positive correlation to the incidence level of A. hilaralis (r = 0.900; p < 0.0001) and to the intensity level of A. hilaralis (r = 0.768; p = 0.0005).
Laju enkapsulasi parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) oleh kutu putih singkong Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) Evie Adriani; Aunu Rauf; Pudjianto Pudjianto
Jurnal Entomologi Indonesia Vol 13 No 3 (2016): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (811.478 KB) | DOI: 10.5994/jei.13.3.147

Abstract

Parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced into Indonesia in early 2014 to control the cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). The objective of study was to determine encapsulation rate of parasitoid A. lopezi by various host instars of P. manihoti. Observation of encapsulation rate was made  by exposing a single mated female of parasitoid on mealybug nymph-1, -2, -3, and adult in a plastic cage for 24 h. Mealybugs then were dissected and number of parasitoid eggs laid and those encapsulated were counted. Study revealed that rate of aggregate encapsulation was highest (8.4%) by adults, followed by nymph-3 (5.8%), nymph-2 (3.1%), and nymph-1 (1.1%). Rate of effective encapsulation by adults was 2.0%, whereas by nymphs about  1.0%. The low rate of encapsulation is believed not to reduce the effectiveness of parasitoid A. lopezi in the biological control of cassava mealybug P. manihoti in Indonesia.
Pengujian kekhususan inang parasitoid Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) pada empat spesies kutu putih yang berasosiasi dengan tanaman singkong Rani Dessy Karyani; Nina Maryana; Aunu Rauf
Jurnal Entomologi Indonesia Vol 13 No 1 (2016): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.493 KB) | DOI: 10.5994/jei.13.1.30

Abstract

A parasitoid, Anagyrus lopezi (De Santis) (Hymenoptera: Encyrtidae) was introduced from Thailand into Indonesia  in early 2014 to control the cassava mealybug Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). To determine its potential uses and effect on non-target species, behavioural observation of the parasitoids were made on four species of mealybugs, i.e. P. manihoti, Paracoccus marginatus Williams-Granara de Willink, Pseudococcus jackbeardsleyi Gimpel-Miller, and Ferrisia virgata Cockerell (Hemiptera: Pseudococcidae). For that purposes, a set of tests were conducted which includes host susceptability, preference, and suitability. Tests were conducted by exposing a female parasitoid to 3rd instar nymphs of each mealybug species in a petri dish. For susceptability test, parasitoid A. lopezi encounterend P. manihoti more often (13.70 ± 7.18 visits per 30 minutes) as compared to P. marginatus (985 ± 10.24), P. jackbeardsleyi (6.60 ± 3.62), and F. virgata (5.75 ± 4.09). So did ovipositor probing occurred more on P. manihoti (8.20 ± 5.68 probes per 30 minutes) than on P. marginatus (0.70 ± 1.84), P. jackbeardsleyi (0.35 ± 0.68), and F. virgata (0.10 ± 0.45). For preference test, host encounter and ovipositor probing by the parasitoid were more common on P. manihoti as opposed to other mealybug species. Out of four mealybug species tested, P. manihoti was the only suitable host for parasitoid development, with the number of progenies emerged 7.40 ± 2.17 individuals per 3 female parasitoids exposed in 24 hour. Host specifity exhibited by parasitoid A. lopezi may prevent adverse effect to other mealybug species inhabiting cassava fields.
Trips (Thysanoptera: Thripidae) yang berasosiasi dengan tanaman hortikultura di Jawa Barat dan kunci identifikasi jenis Vani Nur Oktaviany Subagyo; Purnama Hidayat; Aunu Rauf; Dewi Sartiami
Jurnal Entomologi Indonesia Vol 12 No 2 (2015): Juli
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (819.366 KB) | DOI: 10.5994/jei.12.2.59

Abstract

Most of thrips from Thripidae family are pests on horticultural crops. To date, there are no records in the species richness of thrips in horticultural crops. The aim of the research is to conduct inventory on thrips spp diversity that are associated with horticultural crops in West Java. Sampling were done in several horticultural crops areas in Bogor, Cianjur, and Lembang (West Bandung). The identification process began with sample slide preparations along with the documentation of the character from each species. The identification was done mainly by using the identification program Oztrips and some other published identification keys. Identification key was built based on diagnosis characters of each species. The results found there were 15 species, that are Ceratothripoides brunneus, C. revelatus, Frankliniella intonsa, Megalurothrips typicus, M. usitatus, Scirtothrips dorsalis, Thrips aspinus, T. coloratus, T. hawaiiensis, T. javanicus, T. malloti, T. palmi, T. parvispinus, T. sumatrensis, and T. unispinus. T. aspinus and C. revelatus were new record for Indonesia.
Analysis of Factors Influencing Adoption of Technology in Cocoa Pod Borer Pest Control: A Case Study in West Sulawesi ., Herman; Hutagaol, Parulina; H Sutjahjo, Suharjono; Rauf, Aunu; Priyarsono, D. S
Pelita Perkebunan (Coffee and Cocoa Research Journal) Vol 22, No 3 (2006)
Publisher : Indonesian Coffee and Cocoa Research Institute

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Abstract

Cocoa pod borer is one of the most difficult pests to be controlled, hence it becomes serious threat for sustainable cocoa plantation. The objective of the research is to investigate several factors influencing adoption of innovation technology in pest control by using regression model analysis. The research was carried out in Polewali Mamasa and Mamuju regency, West Sulawesi during February March 2006 using survey method. Number of respondents interviewed in the research was 80 people which was selected by using purposive sampling method. The result shows that CPB in West Sulawesi decreased cocoa productivity 50% (10—90%), caused by less of farmers coordination in controlling CPB and the slowness of technology adoption. Factors which influence farmers knowledge are provision of field school, farmers income and simplicity of the technology. Factors which influence farmers attitude are farmers income knowledge, cultivated cocoa area, provision farmers group and number of family members. Factors which influence farmer action in PsPSP technology adoption is farmer attitude, farmer income, cultivated cocoa area and availability of extension workers in that area. Government’s involvement in accelerating adoption of innovation technology, especially in preparing capable extension workers and provision of subsidized credit for the farmers is inevitable. Key words: cocoa pod borer, adoption of technology, sustainable cacao plantation, Government’s involvement, West Sulawesi
Keragaman Genetik berbagai Isolat Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) dan Virulensinya terhadap Crocidolomia pavonana Trizelia, Trizelia; Santoso, Teguh; Sosromarsono, Soemartono; Rauf, Aunu; Sudirman, Lisdar I
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.998 KB) | DOI: 10.31258/jnat.14.3.176-183

Abstract

The objectives of the research were to study the genetic variability of various Beauveria bassiana (Bals.) Vuill. isolates andvirulence to Crocidolomia pavonana. Thirteen isolates of B. bassiana were isolated from Nilaparvata lugens (Stål),Leptocorisa oratorius (F.), Thrips sp., Hypothenemus hampei (Ferr.), Crocidolomia pavonana (F.), Spodoptera litura (F.),Chrysodeixis chalcites (Esp.), and Riptortus linearis (L.) pests from different geographical locations in Indonesia. Fourteendifferent random primers were used to amplify DNA. Clustering of isolates were obtained using Unweight Pair GroupMethod Avarage (UPGMA). The results showed that only OPA08 primer generated more polymorphic DNA fragments.Result of clustering analysis showed that B. bassiana isolates forms two groups having genetic similarity equal to 55%.Clustering of isolates does not correlate to host type nor geographic origin. Mortality of second instar C. pavonana larvaewas dependent on the fungal isolates. At a concentration of 108 conidia/ml, isolate Bb-La2 had the highest virulence whichcaused 65% mortality of 2nd instar larvae. Isolates of B. bassiana is virulent against C. pavonana larvae present in the samegenotype group.
Komunitas Parasitoid Lalat Pengorok Daun pada Pertanaman Sayuran Dataran Tinggi Rustam, Rusli; Rauf, Aunu; Maryana, Nina; Pudjianto, Pudjianto; Dadang, Dadang
Jurnal Natur Indonesia Vol 11, No 1 (2008)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.174 KB) | DOI: 10.31258/jnat.11.1.40-47

Abstract

Leafminer fly, Liriomyza spp. are widely known as new important pests attacking many vegetable species inhigh land in Indonesia. The objective of the research was to study community of parasitoid leafminer in highaltitude, and parasitization level. This research also looked at more specific on parasitization level caused byOpius chromatomyiae. There were four species of leafminer recorded from the survey, Liriomyza huidobrensis,Liriomyza sativae, Liriomyza chinensis and Chromatomyia horticola. The observation also found eight species ofparasitioid associated with collected leafminers. Six species of recorded parasitoid belonged to family of Eulopidae,and two rest species belonged to Braconidae and Eucoilidae respectively. Those eight species of parasitoids wereHemiptarsenus varicornis, Asecodes deluchii, Neochrysocharis okazakii, Neochrysocharis formosa ,Neocrhysocharis sp. Quadrastichus liriomyzae, Opius chromatomyaie, Gronotoma micromorpha. ParasitoidOpius chromatomyiae and Hemiptarsenus varicornis were found to be dominant in the range altitude between1001-1300 and 1301-1600 asl. From 16 spesies of hosts attacked by leaf miners, only Opius chromatomyiae wasrecorded to have ability to parasitize the leafminer in 13 host species with parasitization level reached 1,84–62,26%.
Co-Authors . Supramana A.A. Ketut Agung Cahyawan W Adriani, Evie AGUSMAN JAYA Ahmad Sifa Anwar, Ruli Budi Abduchalek, Budi Dadan Hidayana DADANG DADANG Dewi Sartiami DJOKO PRIJONO Dominicus Savio Priyarsono Eka Wahyuningsih ELNA KARMAWATI Evie Adriani GILLIAN W. WATSON GREGORY ALLYN EVANS Harwanto Harwanto Hendri Hermawan Herawani, Febrina Herman . Hermanu Triwidodo I Nyoman Widiarta I WAYAN LABA I WAYAN LABA Idham Sakti Harahap IM TRISAWA Indah Putri Januar Yustia Juwita Suri Maharani Kusumah, Yayi Munara Ladja, Fausiah T. LISDAR I. SUDIRMAN Lufthi Rusniarsyah M. SOEHARDJAN M. SOEHARDJAN M. SOEHARDJAN Mahindra Dewi Nur Aisyah MARIETJE PESIRERON Marietje Pesireron Mega wati, Mega NATALIA VON ELLENRIEDER Nelly Mastina Gultom Nfn Pudjianto Nia Kurniawaty Nia Kurniawaty, Nia Nila Wardani Nina Maryana Noor Farikhah Haneda NURARIATY AGUS OLUFEMI JOSEPH ALABI Parulina Hutagaol PUDJIANTO PUDJIANTO Pudjianto Pudjianto, Pudjianto Pudjianto, Nfn Purnama Hidayat PURNAMA HIDAYAT Purnomo Purnomo Qurrotu A’yunin RANGASWAMY MUNIAPPAN Rani Dessy Karyani RAYAPATI ADIKESAVULU NAIDU Rein Estefanus Senewe RIKA RAFFIUDIN Ruly Anwar Rusli Rustam Rusli Rustam Ruth Martha Winnie Samsudin . Samsudin Samsudin Samsudin Samsudin Senewe, Rein Estefanus Senewe, Rein Estefanus Sih Kahono Siswandi Siswandi SITI HERLINDA Siti Masyitah Soemartono Sosromarsono Soemartono Sosromarsono SRI HENDRASTUTI HIDAYAT Sri Utami Sugeng Santoso Sugeng Santoso Sugeng Santoso Suharjono H Sutjahjo Tamrin Abdullah Teguh Santoso Teguh Santoso TRI ASMIRA DAMAYANTI Trizelia . UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO UTOMO KARTOSUWONDO Utomo Kartosuwondo Vani Nur Oktaviany Subagyo Wayan Winasa Widi Rumini Yani Maharani Yani Maharani Yani Maharani, Yani YULIA PUJIASTUTI Yuni Maharani