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TINJAUAN ULANG (REVIEW) TINJAUAN FISIOLOGIS DOMESTIKASI ULAT SUTERA LIAR Attacus atlas (Lepidoptera: Saturniidae) [A Physiological Review on Domestication of Wild Silkworm A. atlas (Lepidoptera: Saturniidae)] Ekastuti, Damiana Rita
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2717.437 KB) | DOI: 10.14203/beritabiologi.v11i2.483

Abstract

Attacus atlas are insects producing silk materials having high economic value. It lives from South East Asia, almost all Indonesia until Australia.The demand of silk from Attacus atlas is quite high (10 tons/month). The A. atlas silk is very exclusive, with permanent in variety colors from light to dark brown. At present, to response the high demand, the people get cocoons from nature. This action could reduce result, and the scarcity of the insect. Beside that, rearing was done by placing the larvae on the trees (outdoor rearing). The outdoor rearing resulted in high mortality because of predators or stress. Better rearing is conducted by indoor rearing, that resulted in high survival rate, and high cocoon or egg production. It is because indoor rearing was supported in better environmental condition than outdoor condition that support physiological process eficiently.
Pertumbuhan Ulat Sutera Bombyx mori (Lepidoptera: Bombycidae) pada Berbagai Sumber Protein dalam Pakannya . Santoso; Damiana Rita Ekastuti
Jurnal Ilmu Pertanian Indonesia Vol. 18 No. 2 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.218 KB)

Abstract

Silkworm rearing has high economical value for producing silk material and other beneficial biological materials such as enzyme, antibacterial agent, and antioxidant. For those purposes, it is important to continuously support high quality feed in the silkworm rearing.This study was aimed to evaluate study the growth of silkworm Bombyx mori fed with artificial diet containing different resources of protein (blood meal or soybean meal). The experiment used completely randomized design 5 five treatments artificial feed formulas and 20 repetitions. Each experiment unit consisted of 1 larvae that was reared individually and given different feed. Formula A: 100% blood meal (BM); B: 75%  (BM) + 25% soybean meal (SM); C: 50% (BM) + 50% (SM); D: 25% (BM)+ 75% (SM); and E: 100% SM. Parameters measured were the feed consumption/larvae, opproximate digestibility, ECI (efficiency of conversion of feed ingested), ECD (efficiency conversion of feed digestibility), and body weight gain in fourth and fifth of the larvae instar. Oxygen consumption were measured on the fifth day of the fifth instar before meal and one hour after meal. The result showed that resource of protein did not significantly affect the parameters on the fourth instar, but significantly affected on the fifth instar larvae. Increment of soybean meal in artificial diet increasing oxygen consumption, and resulted decreassing of ECI and ECD value on the fifth instar larvae.
Preservasi Imago Jantan Ulat Sutera Liar Attacus Atlas (Lepidoptera: Saturniidae) Eko Prasetyo Nugroho; Damiana Rita Ekastuti; Raden Iis Arifiantini
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.27 KB)

Abstract

Wild silkworm (Attacus atlas) has high economic value. There is a problem in rearing wild silkworm Attacus atlas, the emergence of males and females imagoes are not the same. The aim of this research was to study the survival lifetime and the sperm of the male imago. This research used randomized design experiment, with two treatments of storage temperature (room temperature 27 °C and cool storage temperature 5 °C) and eight repetitions. Survival of male imago, sperm survival, and imago body weight had been measured. The results showed that cool temperature (5 °C) could to extend significantly (P<0,01) the survival lifetime of male imago and the sperm of male imago A. atlas up to 11.5 ± 0.7 days. There is a positive correlation between body weight and lifetime of male imago of wild silkworm A. atlas, so the lifetime of male imago is predicable from their body weight.
Pertumbuhan dan Produktivitas Ulat Sutera Bombyx mori L. yang Diberi Pakan Ayam Broiler Aris Kumaidi; Damiana Rita Ekastuti
Acta VETERINARIA Indonesiana Vol. 1 No. 1 (2013): Januari 2013
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.764 KB) | DOI: 10.29244/avi.1.1.1-7

Abstract

Penelitian ini bertujuan mengamati pertumbuhan dan produktivitas ulat sutera Bombyx mori yang diberi pakan buatan dengan formula pakan ayam broiler. Penelitian menggunakan rancangan acak lengkap dengan tujuh kombinasi pakan dan 20 ulangan. Sebagai perlakukan adalah: A, pakan 100% tepung daun murbei (DM); B, 100% DM + 0,5% betasitosterol; C, 75% DM+25% pakan broiler (PB)+ 0,5% betasitosterol; D, 50% DM + 50% PB + 0.5% betasitosterol; E, 25% DM + 75% PB + 0,5% betasitosterol; F, 100% PB + 0,5% betasitosterol; dan G, 100% PB. Satu unit percobaan terdiri dari satu ekor larva yang dipelihara secara individual. Parameter yang diamati adalah: konsumsi bahan kering pakan, kecernaan pakan, bobot badan akhir instar, pertambahan bobot badan, ECI (efficiency conversion of ingested feed), ECD (efficiency conversion of digested feed) periode istar V, bobot pupa, bobot kokon dan persentase kulit kokon. Hasil penelitian menunjukkan bahwa larva yang diberi pakan E, 25% DM + 75% PB + 0,5% betasitosterol, konsumsi pakannya terbanyak, menghasilkan bobot badan akhir instar V, bobot pupa, bobot kokon, dan persentase kulit kokon tertinggi.
Infestasi Cacing Parasitik pada Insang Ikan Mujair (Oreochromis mossambicus) Fitri Dinar Rahayu; Damiana Rita Ekastuti; Risa Tiuria
Acta VETERINARIA Indonesiana Vol. 1 No. 1 (2013): Januari 2013
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.826 KB) | DOI: 10.29244/avi.1.1.8-14

Abstract

Penelitian mengenai infestasi cacing parasitik pada insang ikan mujair (Oreochromis mossambicus) di kolam Kecamatan Dramaga dan kolam Kecamatan Ciomas-Bogor telah dilakukan. Penelitian dilakukan dengan metode deskriptif. Dari 13 sampel ikan mujair yang diperiksa, diperoleh 12 sampel ikan mujair terinfeksi cacing parasitik dan 1 sampel ikan mujair tidak terinfeksi cacing parasitik. Cacing parasitik yang berasal dari kelas Trematoda sub kelas Monogenea yaitu Dactylogyrus sp, Discocotyle sp, dan Gyrodactylus sp serta sub kelas Digenea ditemukan di kolam Kecamatan Dramaga. Cacing parasitik yang berasal dari sub kelas Monogenea yaitu Dactylogyrus sp, Tetraonchus sp dan Gyrodactylus sp ditemukan di kolam Kecamatan Ciomas. Jumlah seluruh cacing parasitik yang diisolasi dari kolam Kecamatan Dramaga berjumlah 50 ekor dengan nilai indeks prevalensi 85,7%. Jumlah seluruh cacing parasitik yang diisolasi dari kolam Kecamatan Ciomas berjumlah 136 ekor dengan nilai indeks prevalensi sebesar 100%. Berat badan ikan akan tergantung pada jenis cacing parasitik yang menginfeksinya dan sifat toleransi individual ikan.
Potensi Ekstrak Kulit Pisang Kepok (Musa paradisiaca forma typica) dan Uli (Musa paradisiaca sapientum) Menaikkan Aktivitas Superoksida Dismutase dan Menurunkan Kadar Malondialdehid Organ Hati Tikus Model Hiperkolesterolemia Azizatul Ulfa; Damiana Rita Ekastuti; Tutik Wresdiyati
Acta VETERINARIA Indonesiana Vol. 8 No. 1 (2020): Januari 2020
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (696.658 KB) | DOI: 10.29244/avi.8.1.40-46

Abstract

Penelitian ini bertujuan untuk menganalisis potensi antioksidan kulit pisang kepok dan kulit pisang uli pada hati tikus hiperkolesterolemia. Kulit pisang kepok dan uli diekstraksi dengan metode maserasi menggunakan pelarut air, etanol 70% dan etanol 96%. Ekstrak diuji fitokimia secara kualitatif. Ekstrak diuji aktivitas antioksidanya menggunakan metode DPPH. Ekstrak dengan nilai IC50 terendah dipilih untuk uji in vivo. Desain penelitian pada uji in vivo meliputi pencegahan dan pengobatan. Uji in vivo dilakukan dengan memberikan varian ekstrak kepada kelompok tikus percobaan. Parameter yang diamati adalah aktivitas SOD dan kadar MDA organ hati. Ekstrak kulit pisang kepok memiliki rendemen 17,97% (pelarut air), 17,18% (pelarut etanol 70%) dan 15,02% (pelarut etanol 96%). Ekstrak kulit pisang uli memiliki rendemen 33,04% (pelarut air), 27,2% (pelarut etanol 70%) dan 34,42% (pelarut etanol 96%). Ekstrak kulit pisang kepok dan uli pada berbagai pelarut memiliki karakteristik warna coklat. Semua ekstrak mengandung senyawa flavonoid. Saponin terdapat pada semua ekstrak kecuali ekstrak kulit pisang uli dengan pelarut etanol 96%. Triterpenoid hanya terkandung pada ekstrak kulit pisang kepok dengan pelarut etanol. Ekstrak kulit pisang kepok dengan pelarut etanol 70% memiliki nilai IC50 terendah (P<0.01) dibandingkan dengan jenis ekstrak lainnya. Kelompok pencegahan dan kelompok pengobatan yang dicekok ekstrak etanol 70% kulit pisang kepok memiliki aktivitas SOD secara nyata lebih tinggi dan kadar MDA lebih rendah dibandingkan kelompok yang tidak dicekok ekstrak (P<0,05). Ekstrak kulit pisang kepok dengan pelarut etanol 70% menunjukkan aktivitas antioksidan yang paling baik. Ekstrak etanol 70% kulit pisang kepok menaikkan aktivitas superoksida dismutase dan menurunkan kadar malondialdehid organ hati tikus percobaan model hiperkolesterolemia baik sebagai pencegahan maupun pengobatan.
Reproductive Performances and Egg Qualities in African Catfish (Clarias gariepinus) Broodstocks Supplemented with Curcumin and Thyroxine Hormone Livana Dethris Rawung; Damiana Rita Ekastuti; Muhammad Zairin Junior; Min Rahminiwati; Ade Sunarma; Wasmen Manalu
Journal Omni-Akuatika Vol 16, No 1 (2020): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.1.696

Abstract

An experiment was designed to study the effects of curcumin and thyroxine hormone supplementation in the diet to improve the reproductive performance of catfish broodstock by improving egg quality that eventually increases the production of seedlings. Catfish used in this experiment were supplemented with curcumin and thyroxine hormone through their feeds for 12 weeks. The results showed that there was no significant difference (p > 0.05) in the hepatosomatic index (HSI), gonadosomatic index (GSI), percentage of gonad maturity, total cholesterol concentration in the spawned eggs, high density lipoprotein (HDL) concentration in the spawned eggs, fertilization rate of spawned eggs, and hatching rate of fertilized eggs.  However, there were significant differences (p < 0.05) in the concentration of vitellogenin in the spawned eggs, egg diameter of the spawned eggs, and the triglycerides contents of spawned eggs. It was concluded that curcumin and thyroxine supplementations of African catfish increased vitellogenin concentrations and diameters of spawned eggs that have great potential to improve the reproductive performance.
The Role of Turmeric Powder Supplementation in Improving Liver Performances to Support Production of Siam Catfish (Pangasianodon hypophthalmus) Cut Dara Dewi; Damiana Rita Ekastuti; Agus Oman Sudrajat; Wasmen Manalu
Journal Omni-Akuatika Vol 14, No 1 (2018): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.597 KB) | DOI: 10.20884/1.oa.2018.14.1.484

Abstract

In oviparous animals, the qualities of offspring are determined by the materials deposited in the developing oocytes that are synthesized and produced by the hepatocytes under the control of estrogen.  This study was designed to evaluate the effect of turmeric powder supplementation on liver performance to support the reproduction performances of catfish (Pangasianodon hypophthalmus). The study used 60 catfish with average body weight of ± 2 kg. The experimental catfish were assigned into a completely randomized design with 4 treatments and each treatment consisted of 15 replications. The treatment was the dose of turmeric powder supplementation in the commercial feed i.e., 0, 240, 480, and 960 mg/100 g feed.  The experimental catfish were fed two times a day in the morning and in the evening as much as 3% of body weight. The results showed that turmeric powder supplementation at a dose of 240 mg/100 g feed could inhibit liver cell destruction in the catfish as was indicated by the increase in body weight, liver weight, and DNA concentrations of liver, as well as the decrease in the liver malondialdehyde, and serum glutamic piruvic transaminase, serum glutamic oxaloacetic transaminase concentrations. Turmeric powder supplementation can be used in oviparous animals and fish to increase liver function and capacity to synthesize vitellogenin to be deposited in the developing follicles during reproduction phase.
6. Erythrocyte Profile of Male Piglet Aged 31-Day Old (Sus scrofa domestica) in the Laboratory Animal Management Unit IPB University Gunanti Gunanti; Damiana Rita Ekastuti; Dwi Utari Rahmiati; Muhammad Fajrul Falah; Erwin Erwin
Jurnal Medika Veterinaria Vol 15, No 1 (2021): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v15i1.22130

Abstract

Domestic pig (Sus scrofa domestica) is well known as animal that used frequently as a research animal due to their similarity to human both in  terms of anatomy and physiology. Information regarding  standard value of domestic piglet’s hematology in Indonesia are still limited. The aim of this study was to obtain erythrocyte profiles of domestic piglets in Animal Laboratory and Research Unit, Bogor Agricultural University. The results of this study are expected to be used as an erythrocyte profiles reference of domestic piglets in Indonesia. Blood samples were collected from six male piglets in Animal Laboratory and Research Unit, Bogor Agricultural University through auricular vein, then the samples were put into a vaccuum tube containing anticoagulant (K3EDTA) for further test using hematology analyzer. The parameters used in this study consist of erythrocyte count, hemoglobin concentration, hematocrit, and erythrocyte indices. Data were analyzed descriptively in the form of mean ± standard deviation (minimum–maximum). The results of this study showed that the average of erythrocyte counts were 6,42 ± 1,03 millions/µL with 8,30 ± 1,51 g/dl hemoglobin concentration, hematocrite 29,80 ± 5,10%, MCV 46,47 ± 1,62 fL, MCH 12,83 ± 0, 65 pg, MCHC 27,75 ± 0,71 g/dL, and RDW 28,87 ± 3,86%.
Keanekaragaman Kapang Patogen dan Non Patogen pada Imago, Kokon, dan Larva Instar Keenam Ulat Sutera Liar Attacus atlas (Lepidoptera: Saturniidae) Agustin Indrawati; Damiana Rita Ekastuti; Erdina Pangestika; Reinilda Alwina
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.546 KB) | DOI: 10.22146/jsv.48022

Abstract

Attacus atlas is one of several mould species in Indonesia known as kupu-kupu gajah. Information about variety of mould is rarely known. The purpose of this research was to obtain data about variety of pathogenic or non pathogenic mould at imago,cocoon, and sixth larvae phase of wild silkworm A. atlas. Mould was isolated from cocoon, integument, alimentary duct and reproduction duct of imago, trachea, midgut and hindgut, also haemolymph of larvae. Isolated mould was cultured on potato dextrose agar. Isolated mould from cocoon and imago was identified by macroscopic and microscopic observation. The results showed that there were two kind of moulds from cocoon which were Fusarium oxysporum  and Aspergillus flavus. There were four kind of moulds from imago A. atlas which were Aspergillus flavus, Aspergillus versicolor, Aspergillus fumigatus, Fusarium dimerum, and Aspergillus sp.There were three kind of moulds from sixth larvae which were Aspergillus fumigatus, Aspergillus flavus, and Fusarium dimerum. The mould which has opportunistic pathogenic for Attacus atlas were Fusarium oxysporum, Aspergillus  flavus, Aspergillus fumigatus and Fusarium dimerum.