Martinius Martinius
Universitas Andalas, Indonesia

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Kemampuan Trichoderma viride Isolat PP2 dalam Mengendalikan Colletotrichum gloeosporioides pada Tanaman Cabai (Capsicum annuum Linnaeus) secara In-Planta Gefi Zulmiati Lannur; Yenni Liswarni; Martinius Martinius
Jurnal Proteksi Tanaman Vol 5 No 2 (2021): December 2021
Publisher : Plant Protection Departement, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.69-76.2021

Abstract

The culture filtrate is a secondary metabolite produced from the multiplication of fungi in a liquid medium during the incubation period, separated between the fungal cell and their supernatants. Trichoderma viride culture filtrate testing has been widely carried out and used as a biocontrol agent. This study aimed to determine the ability of Trichoderma viride PP2 culture filtrate concentration in suppressing anthracnose disease caused by C. gloeosporioides on the red chili pepper. The study was conducted with a randomized block design (RBD) consisting of 5 treatments and five replications. The treatments were the concentration of T. viride PP2 culture filtrate, i.e., 0% (control), 25%, 50%, 75% and 100%. Parameters observed were incubation period, percentage of infected, and infected intensity. The results showed that the application of filtrate of T. viride isolate PP2 with different concentrations has not been able to suppress the development of anthracnose disease caused by C. gloeosporioides in-planta. It is necessary to conduct further research on the effect of temperature and time application on suppressing the filtrate of T. viride isolate PP2.
Antagonism of Endophytic Bacteria and the Consortium Against Culvularia oryzae Bugnic Zurai Resti; Martinius Martinius; Yenny Liswarni
Jurnal Proteksi Tanaman Vol 6 No 1 (2022): June 2022
Publisher : Plant Protection Departement, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.6.1.34-42.2022

Abstract

Endophytic bacteria have several advantages, including controlling plant pathogens and increasing plant growth. As a biological control, endophytic bacteria can suppress the development of plant pathogenic fungi. This study aimed to obtain endophytic bacteria and the consortium of endophytic bacteria that can inhibit the growth of C. oryzae. The study used a Completely Randomized Design (CRD) for studying the ability of endophytic bacteria using eight isolates: Bacillus sp HI, Bacillus sp SJI, B. cereus P14, B. cereus Se07, Serratia marcescens strain JB1E2, S. marces-cens strain JB3, S. marcescens strain ULG1E2 and S. marcescens strain ULG1E4, with three replications. For Studying the ability of endophytic bacteria consortium using six endophytic bacteria consortia and control with three replications. Observations were made on the inhibition growth of C. oryzae. The results showed that all endophytic bacteria and the endophytic bacteria consortium could inhibit C. oryzae, with inhibition of 58.50% – 75.00% and 38.00% – 77.00%. Endophytic bacteria which can significantly suppress the growth of C. oryzae were B. cereus Se07, Bacillus sp HI and Bacillus sp SJI with inhibition of 75.00%, 70.50%, and 70.00%. The endophytic bacteria consortium that had a high ability to suppress the growth of C. oryzae was the consortium of B (S. marcescens strain ULG1E4, S. marcescens strain JB1E3), D (Bacillus sp SJI, Bacillus sp HI, S. marcescenns JB1E3), and G (Bacillus sp SJI, S. marcescens strain ULG1E4), with inhibition of 77.00%, 72.33%, and 71.33%, respectively.